Oiejuq4qtpen0pwrbwkj logo pure biologics

Pure Biologics S.A.

No Reviews Yet
No Completed Orders
Wrocław, PL

About Pure Biologics S.A.

Pure Biologics S.A. is one of the largest Polish biotech companies. Founded in 2010, it is listed on the NewConnect market of the Warsaw Stock Exchange.

 

Pure Biologics’ major R&D activity focuses on bispecific and fusion antibodies, as well...

Show more »

Pure Biologics S.A. is one of the largest Polish biotech companies. Founded in 2010, it is listed on the NewConnect market of the Warsaw Stock Exchange.

 

Pure Biologics’ major R&D activity focuses on bispecific and fusion antibodies, as well as aptamers to be developed into viable candidates for therapeutics in immunooncology (colorectal, breast and lung cancers), and in the treatment of neurological diseases (neuromyelitis optica, myasthenia gravis).

 

To this end, Pure Biologics has created highly efficient platforms to generate specific antibodies and modified aptamers, supported by state-of-the-art assays and analytics.

 

Moreover, Pure Biologics utilizes its broad experience in:

  • antibodies,
  • aptamers,
  • cell and molecular biology,
  • protein and antigen expression and purification,
  • custom assay development,
  • ligand-binding analysis,

offering complex solutions not only as a contract research organization and service provider, but primarily as a professional consultant and R&D partner. Its customers represent a wide variety of environments, ranging from academic collaborations to pharma and biotech companies.

 

Pure Biologics - harnessing the power of antibodies and aptamers!

Show less

Diversity Certificates

Small business

Our Services (44)


ic

Drug Development

Price on request

Show more »

Drug candidate discovery process – antibodies

 

The antibody-based drug candidate discovery process is an extensive research and development undertaking requiring broad expertise, technology and tools to deliver an end result in the form of a potential therapeutic antibody molecule.

 

Relying on its experience in contractual R&D on antibodies, the PureSelect2 platform for in vitro monoclonal antibody selection, numerous proprietary projects on therapeutic antibodies, plus combining the power and tools provided within its other services:

  • antibodies – selection, generation, validation 
  • proteins – expression, purification, production,
  • biophysical analysis – binding, interactions, characterization,

Pure Biologics is positioned to offer complete support in the antibody drug discovery process – from steps of target identification to lead optimization. 

This total R&D service includes among others:

  • antigen production,
  • cell line development,
  • phage display library generation,
  • antibody selection,
  • antibody reformatting and modification,
  • biophysical and cell-based assays,

giving rise to antibody drug lead candidates with pre-defined functions and characteristics.

 

Pure Biologics – providing you complete biotechnological solutions.

Show less

ic

Aptamers

Price on request

Show more »

Aptamers – selection, modification, validation

 

Aptamers are small, single-stranded DNA or RNA molecules, folding into unique, sequence-driven secondary structures, which allows them to bind various targets with high specificity and affinity. Aptamers can be modified to further enhance their binding properties or to add new functions.

 

Pure Biologics offers a complete DNA aptamer selection service – from the raw target to the ready-to-produce aptamer molecule.

 

This complex service is characterized by a fully customizable approach to perfectly fulfill your needs and includes:

  • A full selection campaign against targets such as natural and synthetic molecules, peptides, proteins, bacteria or eukaryotic cells, including stringent counter-selection steps and highly customizable conditions.
  • Next Generation Sequencing of the entire selected pool from every selection round, plus additional background samples.
  • Finding the best candidates in the pool via proprietary bioinformatic tools.
  • Evaluating recommended and custom modifications.
  • Selecting lead aptamers with the best binding characteristics.
  • Biophysical analysis of binding using several orthogonal methods.

Utilizing our proprietary, patented PureApta platform, we can also propose a wide variety of chemical modifications to enhance aptamer stability, resistance, binding properties (via nucleobase modifications), or to introduce novel customer-tailored functions.

The end results are aptamer sequences with defined properties, ready for various assays or other applications.

 

This service can be complemented with Pure Biologics’ other services, including:

  • drug candidate discovery process,
  • proteins (targets) – expression, purification, production,
  • biophysical analysis – binding, interactions, characterization,
  • antibodies – selection, generation, validation,
  • assays/immunoassays – design, development, validation,

delivering you the complete biotechnological solution.

Show less

ic

Monoclonal Antibody (mAb) Development

Price on request

Show more »

Biophysical analysis – binding, interactions, characterization

 

Characterizing molecular interactions is a key analysis in any ligand-receptor based application. Antibodies, aptamers, and their kin – if the goal is to utilize these binding molecules in downstream applications, including assays, drug candidates, effectors or detectors,  reliable and precise data on their affinity, binding coefficients, and kinetics is a must.

 

Relying on its experience in contractual R&D of binding molecules, numerous proprietary projects on therapeutic antibodies and aptamers, Pure Biologics offers comprehensive support in characterizing macromolecules and molecular interactions of binding molecules and their targets.

 

This fully customizable R&D service is based on:

  • SPR – Surface Plasmon Resonance,
  • BLI (Octet) – Bio-layer Interferometry,
  • MST – Microscale Thermophoresis,
  • Macromolecule analysis techniques – DLS (Dynamic Light Scattering), SEC (Size Exclusion Chromatography), DSF (Differential Scanning Fluorometry),
  • Epitope binning – characterizing monoclonal antibody epitopes.
  • Binding kinetics and parameters – kon, koff, KD.
  • Stability of formulations – including folding/unfolding characteristics and Tm,

providing a state-of-the-art, detailed description of molecular binding characteristics and parameters.

 

These data can be directly used to fuel your own research, to satisfy any regulatory proceedings or serve as the basis to launch a co-operation with Pure Biologics in the linked areas of:

  • antibodies – selection, generation, validation,
  • aptamers – selection, modification, validation,
  • proteins – expression, purification, production,
  • assays-immunoassays – design, development, validation,
  • drug candidate discovery process.

Pure Biologics – providing you complete biotechnological solutions.

Show less

ic

Monoclonal Antibody (mAb) Production

Price on request

Show more »

Biophysical analysis – binding, interactions, characterization

 

Characterizing molecular interactions is a key analysis in any ligand-receptor based application. Antibodies, aptamers, and their kin – if the goal is to utilize these binding molecules in downstream applications, including assays, drug candidates, effectors or detectors,  reliable and precise data on their affinity, binding coefficients, and kinetics is a must.

 

Relying on its experience in contractual R&D of binding molecules, numerous proprietary projects on therapeutic antibodies and aptamers, Pure Biologics offers comprehensive support in characterizing macromolecules and molecular interactions of binding molecules and their targets.

 

This fully customizable R&D service is based on:

  • SPR – Surface Plasmon Resonance,
  • BLI (Octet) – Bio-layer Interferometry,
  • MST – Microscale Thermophoresis,
  • Macromolecule analysis techniques – DLS (Dynamic Light Scattering), SEC (Size Exclusion Chromatography), DSF (Differential Scanning Fluorometry),
  • Epitope binning – characterizing monoclonal antibody epitopes.
  • Binding kinetics and parameters – kon, koff, KD.
  • Stability of formulations – including folding/unfolding characteristics and Tm,

providing a state-of-the-art, detailed description of molecular binding characteristics and parameters.

 

These data can be directly used to fuel your own research, to satisfy any regulatory proceedings or serve as the basis to launch a co-operation with Pure Biologics in the linked areas of:

  • antibodies – selection, generation, validation,
  • aptamers – selection, modification, validation,
  • proteins – expression, purification, production,
  • assays-immunoassays – design, development, validation,
  • drug candidate discovery process.

Pure Biologics – providing you complete biotechnological solutions.

Show less

ic

Gene-to-Protein Services

Price on request

Show more »

Proteins – expression, purification, production


Enzymes, proteins and peptides
 – workhorses of cells. Additionally, the components of many products, pharmaceuticals or research and diagnostic tools. Proteins are obvious research, investigation and development targets. However, considering a plethora of proteins and their sources, producing them often proves to be extremely challenging.

 

Pure Biologics offers a comprehensive protein expression, purification and production service – from gene, through expression, optimization and purification, to the fully characterized protein.

 

This service is based on a readily customizable approach to perfectly fulfill your needs and encompasses:

  • Recombinant protein expression and purification in bacteria,
  • Small-scale expression and purification in mammalian cell cultures,
  • Bioreactor-based production,
  • Protein purification from natural sources – bodily fluids, plants, tissues, media, etc.
  • Construct design, optimization and validation,
  • Expression screening and optimization,
  • Protein modifications, tagging, labelling,
  • Protein optimization – solubility, folding, stability,
  • Protein analysis and confirmation – circular dichroism (CD), size exclusion chromatography (SEC), dynamic light scattering (DLS), mass spectrometry (MS) and others.

The end result is a fully characterized and tested protein, ready for various assays or other applications, including therapeutic candidates.

 

This service can be complemented with Pure Biologics’ other services, including:

  • biophysical analysis – binding, interactions, characterization,
  • antibodies – selection, generation, validation,
  • aptamers – selection, modification, validation,
  • assays/immunoassays – design, development, validation,

delivering you the complete biotechnological solution

Show less

ic

Antibody Development

Price on request

Show more »

Biophysical analysis – binding, interactions, characterization

 

Characterizing molecular interactions is a key analysis in any ligand-receptor based application. Antibodies, aptamers, and their kin – if the goal is to utilize these binding molecules in downstream applications, including assays, drug candidates, effectors or detectors,  reliable and precise data on their affinity, binding coefficients, and kinetics is a must.

 

Relying on its experience in contractual R&D of binding molecules, numerous proprietary projects on therapeutic antibodies and aptamers, Pure Biologics offers comprehensive support in characterizing macromolecules and molecular interactions of binding molecules and their targets.

 

This fully customizable R&D service is based on:

  • SPR – Surface Plasmon Resonance,
  • BLI (Octet) – Bio-layer Interferometry,
  • MST – Microscale Thermophoresis,
  • Macromolecule analysis techniques – DLS (Dynamic Light Scattering), SEC (Size Exclusion Chromatography), DSF (Differential Scanning Fluorometry),
  • Epitope binning – characterizing monoclonal antibody epitopes.
  • Binding kinetics and parameters – kon, koff, KD.
  • Stability of formulations – including folding/unfolding characteristics and Tm,

providing a state-of-the-art, detailed description of molecular binding characteristics and parameters.

 

These data can be directly used to fuel your own research, to satisfy any regulatory proceedings or serve as the basis to launch a co-operation with Pure Biologics in the linked areas of:

  • antibodies – selection, generation, validation,
  • aptamers – selection, modification, validation,
  • proteins – expression, purification, production,
  • assays-immunoassays – design, development, validation,
  • drug candidate discovery process.

Pure Biologics – providing you complete biotechnological solutions.

Show less

ic

Immunoassay Development

Price on request

Show more »

Assays-immunoassays – design, development, validation

 


Assays or immunoassays
, like ELISA, found their way to almost every laboratory due to their simplicity, low price and usually instant read-out. Different companies offer thousands of assays capable of detecting and measuring all kinds of molecules. But what if there are none available for your molecule?

 

Relying on its experience in contractual R&D on antibodies and aptamersnumerous proprietary projects and combining the power and tools provided within its other services:

  • antibodies – selection, generation, validation,
  • aptamers – selection, modification, validation,
  • proteins – expression, purification, production,
  • biophysical analysis – binding, interactions, characterization,

Pure Biologics is positioned to offer complete support in aptamer- or antibody-based assay development – from target to a validated assay design. 

This total R&D service includes among others:

  • target (protein) production and/or purification,
  • antibody or aptamer selection, evaluation and validation,
  • custom modifications to meet exact requirements,
  • assay optimization,
  • establishment and validation of assay parameters,
  • complete biophysical analysis,
  • prototype production and testing,

resulting in an immunoassay design meeting pre-defined requirements and characteristics.

 

Pure Biologics – providing you complete biotechnological solutions.

Show less

ic

Antibody Reformatting

Price on request

Show more »

Biophysical analysis – binding, interactions, characterization

 

Characterizing molecular interactions is a key analysis in any ligand-receptor based application. Antibodies, aptamers, and their kin – if the goal is to utilize these binding molecules in downstream applications, including assays, drug candidates, effectors or detectors,  reliable and precise data on their affinity, binding coefficients, and kinetics is a must.

 

Relying on its experience in contractual R&D of binding molecules, numerous proprietary projects on therapeutic antibodies and aptamers, Pure Biologics offers comprehensive support in characterizing macromolecules and molecular interactions of binding molecules and their targets.

 

This fully customizable R&D service is based on:

  • SPR – Surface Plasmon Resonance,
  • BLI (Octet) – Bio-layer Interferometry,
  • MST – Microscale Thermophoresis,
  • Macromolecule analysis techniques – DLS (Dynamic Light Scattering), SEC (Size Exclusion Chromatography), DSF (Differential Scanning Fluorometry),
  • Epitope binning – characterizing monoclonal antibody epitopes.
  • Binding kinetics and parameters – kon, koff, KD.
  • Stability of formulations – including folding/unfolding characteristics and Tm,

providing a state-of-the-art, detailed description of molecular binding characteristics and parameters.

 

These data can be directly used to fuel your own research, to satisfy any regulatory proceedings or serve as the basis to launch a co-operation with Pure Biologics in the linked areas of:

  • antibodies – selection, generation, validation,
  • aptamers – selection, modification, validation,
  • proteins – expression, purification, production,
  • assays-immunoassays – design, development, validation,
  • drug candidate discovery process.

Pure Biologics – providing you complete biotechnological solutions.

Show less

ic

Bispecific Antibody Production

Price on request

Show more »

Biophysical analysis – binding, interactions, characterization

 

Characterizing molecular interactions is a key analysis in any ligand-receptor based application. Antibodies, aptamers, and their kin – if the goal is to utilize these binding molecules in downstream applications, including assays, drug candidates, effectors or detectors,  reliable and precise data on their affinity, binding coefficients, and kinetics is a must.

 

Relying on its experience in contractual R&D of binding molecules, numerous proprietary projects on therapeutic antibodies and aptamers, Pure Biologics offers comprehensive support in characterizing macromolecules and molecular interactions of binding molecules and their targets.

 

This fully customizable R&D service is based on:

  • SPR – Surface Plasmon Resonance,
  • BLI (Octet) – Bio-layer Interferometry,
  • MST – Microscale Thermophoresis,
  • Macromolecule analysis techniques – DLS (Dynamic Light Scattering), SEC (Size Exclusion Chromatography), DSF (Differential Scanning Fluorometry),
  • Epitope binning – characterizing monoclonal antibody epitopes.
  • Binding kinetics and parameters – kon, koff, KD.
  • Stability of formulations – including folding/unfolding characteristics and Tm,

providing a state-of-the-art, detailed description of molecular binding characteristics and parameters.

 

These data can be directly used to fuel your own research, to satisfy any regulatory proceedings or serve as the basis to launch a co-operation with Pure Biologics in the linked areas of:

  • antibodies – selection, generation, validation,
  • aptamers – selection, modification, validation,
  • proteins – expression, purification, production,
  • assays-immunoassays – design, development, validation,
  • drug candidate discovery process.

Pure Biologics – providing you complete biotechnological solutions.

Show less

ic

Ligand Binding Assay (LBA) Bioanalysis

Price on request

Show more »

Biophysical analysis – binding, interactions, characterization

 

Characterizing molecular interactions is a key analysis in any ligand-receptor based application. Antibodies, aptamers, and their kin – if the goal is to utilize these binding molecules in downstream applications, including assays, drug candidates, effectors or detectors,  reliable and precise data on their affinity, binding coefficients, and kinetics is a must.

 

Relying on its experience in contractual R&D of binding molecules, numerous proprietary projects on therapeutic antibodies and aptamers, Pure Biologics offers comprehensive support in characterizing macromolecules and molecular interactions of binding molecules and their targets.

 

This fully customizable R&D service is based on:

  • SPR – Surface Plasmon Resonance,
  • BLI (Octet) – Bio-layer Interferometry,
  • MST – Microscale Thermophoresis,
  • Macromolecule analysis techniques – DLS (Dynamic Light Scattering), SEC (Size Exclusion Chromatography), DSF (Differential Scanning Fluorometry),
  • Epitope binning – characterizing monoclonal antibody epitopes.
  • Binding kinetics and parameters – kon, koff, KD.
  • Stability of formulations – including folding/unfolding characteristics and Tm,

providing a state-of-the-art, detailed description of molecular binding characteristics and parameters.

 

These data can be directly used to fuel your own research, to satisfy any regulatory proceedings or serve as the basis to launch a co-operation with Pure Biologics in the linked areas of:

  • antibodies – selection, generation, validation,
  • aptamers – selection, modification, validation,
  • proteins – expression, purification, production,
  • assays-immunoassays – design, development, validation,
  • drug candidate discovery process.

Pure Biologics – providing you complete biotechnological solutions. 

Show less

ic

Differential Scanning Fluorimetry (DSF)

Differential Scanning Fluorimetry
Price on request

Show more »

Biophysical analysis – binding, interactions, characterization

 

Characterizing molecular interactions is a key analysis in any ligand-receptor based application. Antibodies, aptamers, and their kin – if the goal is to utilize these binding molecules in downstream applications, including assays, drug candidates, effectors or detectors,  reliable and precise data on their affinity, binding coefficients, and kinetics is a must.

 

Relying on its experience in contractual R&D of binding molecules, numerous proprietary projects on therapeutic antibodies and aptamers, Pure Biologics offers comprehensive support in characterizing macromolecules and molecular interactions of binding molecules and their targets.

 

This fully customizable R&D service is based on:

  • SPR – Surface Plasmon Resonance,
  • BLI (Octet) – Bio-layer Interferometry,
  • MST – Microscale Thermophoresis,
  • Macromolecule analysis techniques – DLS (Dynamic Light Scattering), SEC (Size Exclusion Chromatography), DSF (Differential Scanning Fluorometry),
  • Epitope binning – characterizing monoclonal antibody epitopes.
  • Binding kinetics and parameters – kon, koff, KD.
  • Stability of formulations – including folding/unfolding characteristics and Tm,

providing a state-of-the-art, detailed description of molecular binding characteristics and parameters.

 

These data can be directly used to fuel your own research, to satisfy any regulatory proceedings or serve as the basis to launch a co-operation with Pure Biologics in the linked areas of:

  • antibodies – selection, generation, validation,
  • aptamers – selection, modification, validation,
  • proteins – expression, purification, production,
  • assays-immunoassays – design, development, validation,
  • drug candidate discovery process.

Pure Biologics – providing you complete biotechnological solutions. 

Show less

ic

Microscale Thermophoresis

Price on request

Show more »

Biophysical analysis – binding, interactions, characterization

 

Characterizing molecular interactions is a key analysis in any ligand-receptor based application. Antibodies, aptamers, and their kin – if the goal is to utilize these binding molecules in downstream applications, including assays, drug candidates, effectors or detectors,  reliable and precise data on their affinity, binding coefficients, and kinetics is a must.

 

Relying on its experience in contractual R&D of binding molecules, numerous proprietary projects on therapeutic antibodies and aptamers, Pure Biologics offers comprehensive support in characterizing macromolecules and molecular interactions of binding molecules and their targets.

 

This fully customizable R&D service is based on:

  • SPR – Surface Plasmon Resonance,
  • BLI (Octet) – Bio-layer Interferometry,
  • MST – Microscale Thermophoresis,
  • Macromolecule analysis techniques – DLS (Dynamic Light Scattering), SEC (Size Exclusion Chromatography), DSF (Differential Scanning Fluorometry),
  • Epitope binning – characterizing monoclonal antibody epitopes.
  • Binding kinetics and parameters – kon, koff, KD.
  • Stability of formulations – including folding/unfolding characteristics and Tm,

providing a state-of-the-art, detailed description of molecular binding characteristics and parameters.

 

These data can be directly used to fuel your own research, to satisfy any regulatory proceedings or serve as the basis to launch a co-operation with Pure Biologics in the linked areas of:

  • antibodies – selection, generation, validation,
  • aptamers – selection, modification, validation,
  • proteins – expression, purification, production,
  • assays-immunoassays – design, development, validation,
  • drug candidate discovery process.

Pure Biologics – providing you complete biotechnological solutions.

Show less

ic

Biolayer Interferometry

Price on request

Show more »

Biophysical analysis – binding, interactions, characterization

 

Characterizing molecular interactions is a key analysis in any ligand-receptor based application. Antibodies, aptamers, and their kin – if the goal is to utilize these binding molecules in downstream applications, including assays, drug candidates, effectors or detectors,  reliable and precise data on their affinity, binding coefficients, and kinetics is a must.

 

Relying on its experience in contractual R&D of binding molecules, numerous proprietary projects on therapeutic antibodies and aptamers, Pure Biologics offers comprehensive support in characterizing macromolecules and molecular interactions of binding molecules and their targets.

 

This fully customizable R&D service is based on:

  • SPR – Surface Plasmon Resonance,
  • BLI (Octet) – Bio-layer Interferometry,
  • MST – Microscale Thermophoresis,
  • Macromolecule analysis techniques – DLS (Dynamic Light Scattering), SEC (Size Exclusion Chromatography), DSF (Differential Scanning Fluorometry),
  • Epitope binning – characterizing monoclonal antibody epitopes.
  • Binding kinetics and parameters – kon, koff, KD.
  • Stability of formulations – including folding/unfolding characteristics and Tm,

providing a state-of-the-art, detailed description of molecular binding characteristics and parameters.

 

These data can be directly used to fuel your own research, to satisfy any regulatory proceedings or serve as the basis to launch a co-operation with Pure Biologics in the linked areas of:

  • antibodies – selection, generation, validation,
  • aptamers – selection, modification, validation,
  • proteins – expression, purification, production,
  • assays-immunoassays – design, development, validation,
  • drug candidate discovery process.

Pure Biologics – providing you complete biotechnological solutions.

Show less

ic

Aptamer Modification

Price on request

Show more »

Aptamers – selection, modification, validation

 

Aptamers are small, single-stranded DNA or RNA molecules, folding into unique, sequence-driven secondary structures, which allows them to bind various targets with high specificity and affinity. Aptamers can be modified to further enhance their binding properties or to add new functions.

 

Pure Biologics offers a complete DNA aptamer selection service – from the raw target to the ready-to-produce aptamer molecule.

 

This complex service is characterized by a fully customizable approach to perfectly fulfill your needs and includes:

  • A full selection campaign against targets such as natural and synthetic molecules, peptides, proteins, bacteria or eukaryotic cells, including stringent counter-selection steps and highly customizable conditions.
  • Next Generation Sequencing of the entire selected pool from every selection round, plus additional background samples.
  • Finding the best candidates in the pool via proprietary bioinformatic tools.
  • Evaluating recommended and custom modifications.
  • Selecting lead aptamers with the best binding characteristics.
  • Biophysical analysis of binding using several orthogonal methods.

Utilizing our proprietary, patented PureApta platform, we can also propose a wide variety of chemical modifications to enhance aptamer stability, resistance, binding properties (via nucleobase modifications), or to introduce novel customer-tailored functions.

The end results are aptamer sequences with defined properties, ready for various assays or other applications.

 

This service can be complemented with Pure Biologics’ other services, including:

  • drug candidate discovery process,
  • proteins (targets) – expression, purification, production,
  • biophysical analysis – binding, interactions, characterization,
  • antibodies – selection, generation, validation,
  • assays/immunoassays – design, development, validation,

delivering you the complete biotechnological solution.

Show less

ic

Aptamer Screening

Price on request

Show more »

Aptamers – selection, modification, validation

 

Aptamers are small, single-stranded DNA or RNA molecules, folding into unique, sequence-driven secondary structures, which allows them to bind various targets with high specificity and affinity. Aptamers can be modified to further enhance their binding properties or to add new functions.

 

Pure Biologics offers a complete DNA aptamer selection service – from the raw target to the ready-to-produce aptamer molecule.

 

This complex service is characterized by a fully customizable approach to perfectly fulfill your needs and includes:

  • A full selection campaign against targets such as natural and synthetic molecules, peptides, proteins, bacteria or eukaryotic cells, including stringent counter-selection steps and highly customizable conditions.
  • Next Generation Sequencing of the entire selected pool from every selection round, plus additional background samples.
  • Finding the best candidates in the pool via proprietary bioinformatic tools.
  • Evaluating recommended and custom modifications.
  • Selecting lead aptamers with the best binding characteristics.
  • Biophysical analysis of binding using several orthogonal methods.

Utilizing our proprietary, patented PureApta platform, we can also propose a wide variety of chemical modifications to enhance aptamer stability, resistance, binding properties (via nucleobase modifications), or to introduce novel customer-tailored functions.

The end results are aptamer sequences with defined properties, ready for various assays or other applications.

 

This service can be complemented with Pure Biologics’ other services, including:

  • drug candidate discovery process,
  • proteins (targets) – expression, purification, production,
  • biophysical analysis – binding, interactions, characterization,
  • antibodies – selection, generation, validation,
  • assays/immunoassays – design, development, validation,

delivering you the complete biotechnological solution.

Show less

ic

Aptamer Selection Service

Price on request

Show more »

Aptamers – selection, modification, validation

 

Aptamers are small, single-stranded DNA or RNA molecules, folding into unique, sequence-driven secondary structures, which allows them to bind various targets with high specificity and affinity. Aptamers can be modified to further enhance their binding properties or to add new functions.

 

Pure Biologics offers a complete DNA aptamer selection service – from the raw target to the ready-to-produce aptamer molecule.

 

This complex service is characterized by a fully customizable approach to perfectly fulfill your needs and includes:

  • A full selection campaign against targets such as natural and synthetic molecules, peptides, proteins, bacteria or eukaryotic cells, including stringent counter-selection steps and highly customizable conditions.
  • Next Generation Sequencing of the entire selected pool from every selection round, plus additional background samples.
  • Finding the best candidates in the pool via proprietary bioinformatic tools.
  • Evaluating recommended and custom modifications.
  • Selecting lead aptamers with the best binding characteristics.
  • Biophysical analysis of binding using several orthogonal methods.

Utilizing our proprietary, patented PureApta platform, we can also propose a wide variety of chemical modifications to enhance aptamer stability, resistance, binding properties (via nucleobase modifications), or to introduce novel customer-tailored functions.

The end results are aptamer sequences with defined properties, ready for various assays or other applications.

 

This service can be complemented with Pure Biologics’ other services, including:

  • drug candidate discovery process,
  • proteins (targets) – expression, purification, production,
  • biophysical analysis – binding, interactions, characterization,
  • antibodies – selection, generation, validation,
  • assays/immunoassays – design, development, validation,

delivering you the complete biotechnological solution.

Show less

ic

Antibody Discovery

Price on request

Show more »

Antibodies – selection, generation, validation

 

 

Antibodies used in research and as therapeutics are raised mostly in vivo by immunization of animals and subsequent selection by use of hybridoma technology. Antibodies obtained using these strategies must be then “humanized” in order to be immunologically compatible with humans.

 

Pure Biologics offers a complete monoclonal antibody selection service – from the raw target to the fully characterized antibody – from an in vitro selection strategy using phage display technology with no further need to “humanize”.

 

This complex service utilizes our proprietary PureSelect2 platform for selection of high-affinity human recombinant antibodies against various molecular targets, including small molecules, peptides, proteins or whole cells expressing the antigen of interest.

The service is based on a readily customizable approach to perfectly fulfill your needs and it relies on:

  • Selecting interacting clones in a high-throughput format via phage display – combining extensive depletion steps with a positive selection on solid phase or in solution.
  • Constructing a secondary library for re-selection (maturation) – further enhancing the affinity, stability or compatibility with humans.
  • Transforming antibody fragments into other formats – full IgG, Fab, scFv-dimer and others.
  • Biophysical analysis of binding using several orthogonal methods.
  • Chemically modifying and improving antibodies – creating conjugates or tagged antibodies, removing N-glycosylation sites.
  • Validating antibodies – via ELISA, in a cellular context, via flow cytometry or with dedicated functional tests (e.g. immunoassays).

The end results are fully characterized and validated antibodies, ready for various assays or other applications, including therapeutic candidates.

 

This service can be complemented with Pure Biologics’ other services, including:

  • drug candidate discovery process,
  • proteins (e.g. targets) – expression, purification, production,
  • biophysical analysis – binding, interactions, characterization,
  • aptamers – selection, modification, validation,
  • assays/immunoassays – design, development, validation,

delivering you the complete biotechnological solution

Show less

ic

Recombinant Protein Expression

Price on request

Show more »

Proteins – expression, purification, production


Enzymes, proteins and peptides
 – workhorses of cells. Additionally, the components of many products, pharmaceuticals or research and diagnostic tools. Proteins are obvious research, investigation and development targets. However, considering a plethora of proteins and their sources, producing them often proves to be extremely challenging.

 

Pure Biologics offers a comprehensive protein expression, purification and production service – from gene, through expression, optimization and purification, to the fully characterized protein.

 

This service is based on a readily customizable approach to perfectly fulfill your needs and encompasses:

  • Recombinant protein expression and purification in bacteria,
  • Small-scale expression and purification in mammalian cell cultures,
  • Bioreactor-based production,
  • Protein purification from natural sources – bodily fluids, plants, tissues, media, etc.
  • Construct design, optimization and validation,
  • Expression screening and optimization,
  • Protein modifications, tagging, labelling,
  • Protein optimization – solubility, folding, stability,
  • Protein analysis and confirmation – circular dichroism (CD), size exclusion chromatography (SEC), dynamic light scattering (DLS), mass spectrometry (MS) and others.

The end result is a fully characterized and tested protein, ready for various assays or other applications, including therapeutic candidates.

 

This service can be complemented with Pure Biologics’ other services, including:

  • biophysical analysis – binding, interactions, characterization,
  • antibodies – selection, generation, validation,
  • aptamers – selection, modification, validation,
  • assays/immunoassays – design, development, validation,

delivering you the complete biotechnological solution.

Show less

ic

Antigen Expression and Purification

Price on request

Show more »

Proteins – expression, purification, production


Enzymes, proteins and peptides
 – workhorses of cells. Additionally, the components of many products, pharmaceuticals or research and diagnostic tools. Proteins are obvious research, investigation and development targets. However, considering a plethora of proteins and their sources, producing them often proves to be extremely challenging.

 

Pure Biologics offers a comprehensive protein expression, purification and production service – from gene, through expression, optimization and purification, to the fully characterized protein.

 

This service is based on a readily customizable approach to perfectly fulfill your needs and encompasses:

  • Recombinant protein expression and purification in bacteria,
  • Small-scale expression and purification in mammalian cell cultures,
  • Bioreactor-based production,
  • Protein purification from natural sources – bodily fluids, plants, tissues, media, etc.
  • Construct design, optimization and validation,
  • Expression screening and optimization,
  • Protein modifications, tagging, labelling,
  • Protein optimization – solubility, folding, stability,
  • Protein analysis and confirmation – circular dichroism (CD), size exclusion chromatography (SEC), dynamic light scattering (DLS), mass spectrometry (MS) and others.

The end result is a fully characterized and tested protein, ready for various assays or other applications, including therapeutic candidates.

 

This service can be complemented with Pure Biologics’ other services, including:

  • biophysical analysis – binding, interactions, characterization,
  • antibodies – selection, generation, validation,
  • aptamers – selection, modification, validation,
  • assays/immunoassays – design, development, validation,

delivering you the complete biotechnological solution.

Show less

ic

Antibody Screening

Price on request

Show more »

Antibodies – selection, generation, validation

 

 

Antibodies used in research and as therapeutics are raised mostly in vivo by immunization of animals and subsequent selection by use of hybridoma technology. Antibodies obtained using these strategies must be then “humanized” in order to be immunologically compatible with humans.

 

Pure Biologics offers a complete monoclonal antibody selection service – from the raw target to the fully characterized antibody – from an in vitro selection strategy using phage display technology with no further need to “humanize”.

 

This complex service utilizes our proprietary PureSelect2 platform for selection of high-affinity human recombinant antibodies against various molecular targets, including small molecules, peptides, proteins or whole cells expressing the antigen of interest.

The service is based on a readily customizable approach to perfectly fulfill your needs and it relies on:

  • Selecting interacting clones in a high-throughput format via phage display – combining extensive depletion steps with a positive selection on solid phase or in solution.
  • Constructing a secondary library for re-selection (maturation) – further enhancing the affinity, stability or compatibility with humans.
  • Transforming antibody fragments into other formats – full IgG, Fab, scFv-dimer and others.
  • Biophysical analysis of binding using several orthogonal methods.
  • Chemically modifying and improving antibodies – creating conjugates or tagged antibodies, removing N-glycosylation sites.
  • Validating antibodies – via ELISA, in a cellular context, via flow cytometry or with dedicated functional tests (e.g. immunoassays).

The end results are fully characterized and validated antibodies, ready for various assays or other applications, including therapeutic candidates.

 

This service can be complemented with Pure Biologics’ other services, including:

  • drug candidate discovery process,
  • proteins (e.g. targets) – expression, purification, production,
  • biophysical analysis – binding, interactions, characterization,
  • aptamers – selection, modification, validation,
  • assays/immunoassays – design, development, validation,

delivering you the complete biotechnological solution.

Show less

ic

Phage Display Library Construction and Screening

Price on request

Show more »

Antibodies – selection, generation, validation

 

 

Antibodies used in research and as therapeutics are raised mostly in vivo by immunization of animals and subsequent selection by use of hybridoma technology. Antibodies obtained using these strategies must be then “humanized” in order to be immunologically compatible with humans.

 

Pure Biologics offers a complete monoclonal antibody selection service – from the raw target to the fully characterized antibody – from an in vitro selection strategy using phage display technology with no further need to “humanize”.

 

This complex service utilizes our proprietary PureSelect2 platform for selection of high-affinity human recombinant antibodies against various molecular targets, including small molecules, peptides, proteins or whole cells expressing the antigen of interest.

The service is based on a readily customizable approach to perfectly fulfill your needs and it relies on:

  • Selecting interacting clones in a high-throughput format via phage display – combining extensive depletion steps with a positive selection on solid phase or in solution.
  • Constructing a secondary library for re-selection (maturation) – further enhancing the affinity, stability or compatibility with humans.
  • Transforming antibody fragments into other formats – full IgG, Fab, scFv-dimer and others.
  • Biophysical analysis of binding using several orthogonal methods.
  • Chemically modifying and improving antibodies – creating conjugates or tagged antibodies, removing N-glycosylation sites.
  • Validating antibodies – via ELISA, in a cellular context, via flow cytometry or with dedicated functional tests (e.g. immunoassays).

The end results are fully characterized and validated antibodies, ready for various assays or other applications, including therapeutic candidates.

 

This service can be complemented with Pure Biologics’ other services, including:

  • drug candidate discovery process,
  • proteins (e.g. targets) – expression, purification, production,
  • biophysical analysis – binding, interactions, characterization,
  • aptamers – selection, modification, validation,
  • assays/immunoassays – design, development, validation,

delivering you the complete biotechnological solution.

Show less

ic

Bacterial Protein Expression

Price on request

Show more »

Proteins – expression, purification, production


Enzymes, proteins and peptides
 – workhorses of cells. Additionally, the components of many products, pharmaceuticals or research and diagnostic tools. Proteins are obvious research, investigation and development targets. However, considering a plethora of proteins and their sources, producing them often proves to be extremely challenging.

 

Pure Biologics offers a comprehensive protein expression, purification and production service – from gene, through expression, optimization and purification, to the fully characterized protein.

 

This service is based on a readily customizable approach to perfectly fulfill your needs and encompasses:

  • Recombinant protein expression and purification in bacteria,
  • Small-scale expression and purification in mammalian cell cultures,
  • Bioreactor-based production,
  • Protein purification from natural sources – bodily fluids, plants, tissues, media, etc.
  • Construct design, optimization and validation,
  • Expression screening and optimization,
  • Protein modifications, tagging, labelling,
  • Protein optimization – solubility, folding, stability,
  • Protein analysis and confirmation – circular dichroism (CD), size exclusion chromatography (SEC), dynamic light scattering (DLS), mass spectrometry (MS) and others.

The end result is a fully characterized and tested protein, ready for various assays or other applications, including therapeutic candidates.

 

This service can be complemented with Pure Biologics’ other services, including:

  • biophysical analysis – binding, interactions, characterization,
  • antibodies – selection, generation, validation,
  • aptamers – selection, modification, validation,
  • assays/immunoassays – design, development, validation,

delivering you the complete biotechnological solution.

Show less

ic

Protein Expression in Mammalian Cells

Mammalian cell based recombinant protein production
Price on request

Show more »

Proteins – expression, purification, production


Enzymes, proteins and peptides
 – workhorses of cells. Additionally, the components of many products, pharmaceuticals or research and diagnostic tools. Proteins are obvious research, investigation and development targets. However, considering a plethora of proteins and their sources, producing them often proves to be extremely challenging.

 

Pure Biologics offers a comprehensive protein expression, purification and production service – from gene, through expression, optimization and purification, to the fully characterized protein.

 

This service is based on a readily customizable approach to perfectly fulfill your needs and encompasses:

  • Recombinant protein expression and purification in bacteria,
  • Small-scale expression and purification in mammalian cell cultures,
  • Bioreactor-based production,
  • Protein purification from natural sources – bodily fluids, plants, tissues, media, etc.
  • Construct design, optimization and validation,
  • Expression screening and optimization,
  • Protein modifications, tagging, labelling,
  • Protein optimization – solubility, folding, stability,
  • Protein analysis and confirmation – circular dichroism (CD), size exclusion chromatography (SEC), dynamic light scattering (DLS), mass spectrometry (MS) and others.

The end result is a fully characterized and tested protein, ready for various assays or other applications, including therapeutic candidates.

 

This service can be complemented with Pure Biologics’ other services, including:

  • biophysical analysis – binding, interactions, characterization,
  • antibodies – selection, generation, validation,
  • aptamers – selection, modification, validation,
  • assays/immunoassays – design, development, validation,

delivering you the complete biotechnological solution.

Show less

ic

Protein Purification

Price on request

Show more »

Proteins – expression, purification, production


Enzymes, proteins and peptides
 – workhorses of cells. Additionally, the components of many products, pharmaceuticals or research and diagnostic tools. Proteins are obvious research, investigation and development targets. However, considering a plethora of proteins and their sources, producing them often proves to be extremely challenging.

 

Pure Biologics offers a comprehensive protein expression, purification and production service – from gene, through expression, optimization and purification, to the fully characterized protein.

 

This service is based on a readily customizable approach to perfectly fulfill your needs and encompasses:

  • Recombinant protein expression and purification in bacteria,
  • Small-scale expression and purification in mammalian cell cultures,
  • Bioreactor-based production,
  • Protein purification from natural sources – bodily fluids, plants, tissues, media, etc.
  • Construct design, optimization and validation,
  • Expression screening and optimization,
  • Protein modifications, tagging, labelling,
  • Protein optimization – solubility, folding, stability,
  • Protein analysis and confirmation – circular dichroism (CD), size exclusion chromatography (SEC), dynamic light scattering (DLS), mass spectrometry (MS) and others.

The end result is a fully characterized and tested protein, ready for various assays or other applications, including therapeutic candidates.

 

This service can be complemented with Pure Biologics’ other services, including:

  • biophysical analysis – binding, interactions, characterization,
  • antibodies – selection, generation, validation,
  • aptamers – selection, modification, validation,
  • assays/immunoassays – design, development, validation,

delivering you the complete biotechnological solution.

Show less

ic

Protein Refolding

Price on request

Show more »

Proteins – expression, purification, production


Enzymes, proteins and peptides
 – workhorses of cells. Additionally, the components of many products, pharmaceuticals or research and diagnostic tools. Proteins are obvious research, investigation and development targets. However, considering a plethora of proteins and their sources, producing them often proves to be extremely challenging.

 

Pure Biologics offers a comprehensive protein expression, purification and production service – from gene, through expression, optimization and purification, to the fully characterized protein.

 

This service is based on a readily customizable approach to perfectly fulfill your needs and encompasses:

  • Recombinant protein expression and purification in bacteria,
  • Small-scale expression and purification in mammalian cell cultures,
  • Bioreactor-based production,
  • Protein purification from natural sources – bodily fluids, plants, tissues, media, etc.
  • Construct design, optimization and validation,
  • Expression screening and optimization,
  • Protein modifications, tagging, labelling,
  • Protein optimization – solubility, folding, stability,
  • Protein analysis and confirmation – circular dichroism (CD), size exclusion chromatography (SEC), dynamic light scattering (DLS), mass spectrometry (MS) and others.

The end result is a fully characterized and tested protein, ready for various assays or other applications, including therapeutic candidates.

 

This service can be complemented with Pure Biologics’ other services, including:

  • biophysical analysis – binding, interactions, characterization,
  • antibodies – selection, generation, validation,
  • aptamers – selection, modification, validation,
  • assays/immunoassays – design, development, validation,

delivering you the complete biotechnological solution.

Show less

ic

Antibody Engineering

Price on request

Show more »

Antibodies – selection, generation, validation

 

 

Antibodies used in research and as therapeutics are raised mostly in vivo by immunization of animals and subsequent selection by use of hybridoma technology. Antibodies obtained using these strategies must be then “humanized” in order to be immunologically compatible with humans.

 

Pure Biologics offers a complete monoclonal antibody selection service – from the raw target to the fully characterized antibody – from an in vitro selection strategy using phage display technology with no further need to “humanize”.

 

This complex service utilizes our proprietary PureSelect2 platform for selection of high-affinity human recombinant antibodies against various molecular targets, including small molecules, peptides, proteins or whole cells expressing the antigen of interest.

The service is based on a readily customizable approach to perfectly fulfill your needs and it relies on:

  • Selecting interacting clones in a high-throughput format via phage display – combining extensive depletion steps with a positive selection on solid phase or in solution.
  • Constructing a secondary library for re-selection (maturation) – further enhancing the affinity, stability or compatibility with humans.
  • Transforming antibody fragments into other formats – full IgG, Fab, scFv-dimer and others.
  • Biophysical analysis of binding using several orthogonal methods.
  • Chemically modifying and improving antibodies – creating conjugates or tagged antibodies, removing N-glycosylation sites.
  • Validating antibodies – via ELISA, in a cellular context, via flow cytometry or with dedicated functional tests (e.g. immunoassays).

The end results are fully characterized and validated antibodies, ready for various assays or other applications, including therapeutic candidates.

 

This service can be complemented with Pure Biologics’ other services, including:

  • drug candidate discovery process,
  • proteins (e.g. targets) – expression, purification, production,
  • biophysical analysis – binding, interactions, characterization,
  • aptamers – selection, modification, validation,
  • assays/immunoassays – design, development, validation,

delivering you the complete biotechnological solution.

Show less

ic

Recombinant Antibody Production

Price on request

Show more »

Antibodies – selection, generation, validation

 

 

Antibodies used in research and as therapeutics are raised mostly in vivo by immunization of animals and subsequent selection by use of hybridoma technology. Antibodies obtained using these strategies must be then “humanized” in order to be immunologically compatible with humans.

 

Pure Biologics offers a complete monoclonal antibody selection service – from the raw target to the fully characterized antibody – from an in vitro selection strategy using phage display technology with no further need to “humanize”.

 

This complex service utilizes our proprietary PureSelect2 platform for selection of high-affinity human recombinant antibodies against various molecular targets, including small molecules, peptides, proteins or whole cells expressing the antigen of interest.

The service is based on a readily customizable approach to perfectly fulfill your needs and it relies on:

  • Selecting interacting clones in a high-throughput format via phage display – combining extensive depletion steps with a positive selection on solid phase or in solution.
  • Constructing a secondary library for re-selection (maturation) – further enhancing the affinity, stability or compatibility with humans.
  • Transforming antibody fragments into other formats – full IgG, Fab, scFv-dimer and others.
  • Biophysical analysis of binding using several orthogonal methods.
  • Chemically modifying and improving antibodies – creating conjugates or tagged antibodies, removing N-glycosylation sites.
  • Validating antibodies – via ELISA, in a cellular context, via flow cytometry or with dedicated functional tests (e.g. immunoassays).

The end results are fully characterized and validated antibodies, ready for various assays or other applications, including therapeutic candidates.

 

This service can be complemented with Pure Biologics’ other services, including:

  • drug candidate discovery process,
  • proteins (e.g. targets) – expression, purification, production,
  • biophysical analysis – binding, interactions, characterization,
  • aptamers – selection, modification, validation,
  • assays/immunoassays – design, development, validation,

delivering you the complete biotechnological solution.

Show less

ic

Bioanalytical Assays

Price on request

Show more »

Biophysical analysis – binding, interactions, characterization

 

Characterizing molecular interactions is a key analysis in any ligand-receptor based application. Antibodies, aptamers, and their kin – if the goal is to utilize these binding molecules in downstream applications, including assays, drug candidates, effectors or detectors,  reliable and precise data on their affinity, binding coefficients, and kinetics is a must.

 

Relying on its experience in contractual R&D of binding molecules, numerous proprietary projects on therapeutic antibodies and aptamers, Pure Biologics offers comprehensive support in characterizing macromolecules and molecular interactions of binding molecules and their targets.

 

This fully customizable R&D service is based on:

  • SPR – Surface Plasmon Resonance,
  • BLI (Octet) – Bio-layer Interferometry,
  • MST – Microscale Thermophoresis,
  • Macromolecule analysis techniques – DLS (Dynamic Light Scattering), SEC (Size Exclusion Chromatography), DSF (Differential Scanning Fluorometry),
  • Epitope binning – characterizing monoclonal antibody epitopes.
  • Binding kinetics and parameters – kon, koff, KD.
  • Stability of formulations – including folding/unfolding characteristics and Tm,

providing a state-of-the-art, detailed description of molecular binding characteristics and parameters.

 

These data can be directly used to fuel your own research, to satisfy any regulatory proceedings or serve as the basis to launch a co-operation with Pure Biologics in the linked areas of:

  • antibodies – selection, generation, validation,
  • aptamers – selection, modification, validation,
  • proteins – expression, purification, production,
  • assays-immunoassays – design, development, validation,
  • drug candidate discovery process.

Pure Biologics – providing you complete biotechnological solutions.

Show less

ic

Custom Cell-Based Assay Development

Price on request

Comprehensive assay development services based on clients’ specifications and needs.

Kinetics and biophysics of protein-protein, protein-ligand & DNA interactions.

In-house capabilities include:
• Surface plasmon resonance (SPR) analysis:
(i) kinetics of interaction (Kd, kon, koff),
(ii) affinity and avidity assessments... Show more »

Comprehensive assay development services based on clients’ specifications and needs.

Kinetics and biophysics of protein-protein, protein-ligand & DNA interactions.

In-house capabilities include:
• Surface plasmon resonance (SPR) analysis:
(i) kinetics of interaction (Kd, kon, koff),
(ii) affinity and avidity assessments in high throughput mode;

• Isothermal Titration Calorimetry (ITC) analysis:
(i) mechanism of interaction (Kd, DH, DS, stoichiometry);

• ELISA:
(i) assay development and validation,
(ii) custom recombinant antibody generation by phage display if necessary;

• Fluorescence intensity and polarization:
(i) kinetics of interaction (Kd),
(ii) fluorescent probe assay suitability assessment;

• Enzyme kinetics parameterization (kcat, Vmax, Km, IC50, Ki)

Show less

ic

Therapeutic Monoclonal Antibody (mAb) Development

Price on request

Show more »

Drug candidate discovery process – antibodies

 

The antibody-based drug candidate discovery process is an extensive research and development undertaking requiring broad expertise, technology and tools to deliver an end result in the form of a potential therapeutic antibody molecule.

 

Relying on its experience in contractual R&D on antibodies, the PureSelect2 platform for in vitro monoclonal antibody selection, numerous proprietary projects on therapeutic antibodies, plus combining the power and tools provided within its other services:

  • antibodies – selection, generation, validation 
  • proteins – expression, purification, production,
  • biophysical analysis – binding, interactions, characterization,

Pure Biologics is positioned to offer complete support in the antibody drug discovery process – from steps of target identification to lead optimization. 

This total R&D service includes among others:

  • antigen production,
  • cell line development,
  • phage display library generation,
  • antibody selection,
  • antibody reformatting and modification,
  • biophysical and cell-based assays,

giving rise to antibody drug lead candidates with pre-defined functions and characteristics.

 

Pure Biologics – providing you complete biotechnological solutions.

Show less

ic

Bioanalysis

Price on request
Request a quote for more information about this service.

ic

Protein Services

Price on request
Request a quote for more information about this service.

ic

Biochemistry & Molecular Biology

Price on request
Request a quote for more information about this service.

ic

Antibody Services

Price on request

Show more »

Antibodies – selection, generation, validation

 

 

Antibodies used in research and as therapeutics are raised mostly in vivo by immunization of animals and subsequent selection by use of hybridoma technology. Antibodies obtained using these strategies must be then “humanized” in order to be immunologically compatible with humans.

 

Pure Biologics offers a complete monoclonal antibody selection service – from the raw target to the fully characterized antibody – from an in vitro selection strategy using phage display technology with no further need to “humanize”.

 

This complex service utilizes our proprietary PureSelect2 platform for selection of high-affinity human recombinant antibodies against various molecular targets, including small molecules, peptides, proteins or whole cells expressing the antigen of interest.

The service is based on a readily customizable approach to perfectly fulfill your needs and it relies on:

  • Selecting interacting clones in a high-throughput format via phage display – combining extensive depletion steps with a positive selection on solid phase or in solution.
  • Constructing a secondary library for re-selection (maturation) – further enhancing the affinity, stability or compatibility with humans.
  • Transforming antibody fragments into other formats – full IgG, Fab, scFv-dimer and others.
  • Biophysical analysis of binding using several orthogonal methods.
  • Chemically modifying and improving antibodies – creating conjugates or tagged antibodies, removing N-glycosylation sites.
  • Validating antibodies – via ELISA, in a cellular context, via flow cytometry or with dedicated functional tests (e.g. immunoassays).

The end results are fully characterized and validated antibodies, ready for various assays or other applications, including therapeutic candidates.

 

This service can be complemented with Pure Biologics’ other services, including:

  • drug candidate discovery process,
  • proteins (e.g. targets) – expression, purification, production,
  • biophysical analysis – binding, interactions, characterization,
  • aptamers – selection, modification, validation,
  • assays/immunoassays – design, development, validation,

delivering you the complete biotechnological solution.

Show less

ic

Protein Production

Price on request

Show more »

Proteins – expression, purification, production


Enzymes, proteins and peptides
 – workhorses of cells. Additionally, the components of many products, pharmaceuticals or research and diagnostic tools. Proteins are obvious research, investigation and development targets. However, considering a plethora of proteins and their sources, producing them often proves to be extremely challenging.

 

Pure Biologics offers a comprehensive protein expression, purification and production service – from gene, through expression, optimization and purification, to the fully characterized protein.

 

This service is based on a readily customizable approach to perfectly fulfill your needs and encompasses:

  • Recombinant protein expression and purification in bacteria,
  • Small-scale expression and purification in mammalian cell cultures,
  • Bioreactor-based production,
  • Protein purification from natural sources – bodily fluids, plants, tissues, media, etc.
  • Construct design, optimization and validation,
  • Expression screening and optimization,
  • Protein modifications, tagging, labelling,
  • Protein optimization – solubility, folding, stability,
  • Protein analysis and confirmation – circular dichroism (CD), size exclusion chromatography (SEC), dynamic light scattering (DLS), mass spectrometry (MS) and others.

The end result is a fully characterized and tested protein, ready for various assays or other applications, including therapeutic candidates.

 

This service can be complemented with Pure Biologics’ other services, including:

  • biophysical analysis – binding, interactions, characterization,
  • antibodies – selection, generation, validation,
  • aptamers – selection, modification, validation,
  • assays/immunoassays – design, development, validation,

delivering you the complete biotechnological solution.

Show less

ic

Cell-Based Assays

Price on request
Request a quote for more information about this service.

ic

Pharmacology & Toxicology

Price on request
Request a quote for more information about this service.

ic

Specialized Cell-Based Assays

Price on request
Request a quote for more information about this service.

ic

Surface Plasmon Resonance (SPR)

Price on request
Request a quote for more information about this service.

ic

Protein-Protein Interaction Analysis

Price on request
Request a quote for more information about this service.

ic

Immunoassays

Price on request

Show more »

Assays-immunoassays – design, development, validation

 


Assays or immunoassays
, like ELISA, found their way to almost every laboratory due to their simplicity, low price and usually instant read-out. Different companies offer thousands of assays capable of detecting and measuring all kinds of molecules. But what if there are none available for your molecule?

 

Relying on its experience in contractual R&D on antibodies and aptamersnumerous proprietary projects and combining the power and tools provided within its other services:

  • antibodies – selection, generation, validation,
  • aptamers – selection, modification, validation,
  • proteins – expression, purification, production,
  • biophysical analysis – binding, interactions, characterization,

Pure Biologics is positioned to offer complete support in aptamer- or antibody-based assay development – from target to a validated assay design. 

This total R&D service includes among others:

  • target (protein) production and/or purification,
  • antibody or aptamer selection, evaluation and validation,
  • custom modifications to meet exact requirements,
  • assay optimization,
  • establishment and validation of assay parameters,
  • complete biophysical analysis,
  • prototype production and testing,

resulting in an immunoassay design meeting pre-defined requirements and characteristics.

 

Pure Biologics – providing you complete biotechnological solutions.

Show less

ic

Biopharmaceutical Development

Price on request
Request a quote for more information about this service.

ic

Recombinant Antigen Development

Price on request
Request a quote for more information about this service.

ic

Protein Expression Screening and Optimization

Price on request

Show more »

Proteins – expression, purification, production


Enzymes, proteins and peptides
 – workhorses of cells. Additionally, the components of many products, pharmaceuticals or research and diagnostic tools. Proteins are obvious research, investigation and development targets. However, considering a plethora of proteins and their sources, producing them often proves to be extremely challenging.

 

Pure Biologics offers a comprehensive protein expression, purification and production service – from gene, through expression, optimization and purification, to the fully characterized protein.

 

This service is based on a readily customizable approach to perfectly fulfill your needs and encompasses:

  • Recombinant protein expression and purification in bacteria,
  • Small-scale expression and purification in mammalian cell cultures,
  • Bioreactor-based production,
  • Protein purification from natural sources – bodily fluids, plants, tissues, media, etc.
  • Construct design, optimization and validation,
  • Expression screening and optimization,
  • Protein modifications, tagging, labelling,
  • Protein optimization – solubility, folding, stability,
  • Protein analysis and confirmation – circular dichroism (CD), size exclusion chromatography (SEC), dynamic light scattering (DLS), mass spectrometry (MS) and others.

The end result is a fully characterized and tested protein, ready for various assays or other applications, including therapeutic candidates.

 

This service can be complemented with Pure Biologics’ other services, including:

  • biophysical analysis – binding, interactions, characterization,
  • antibodies – selection, generation, validation,
  • aptamers – selection, modification, validation,
  • assays/immunoassays – design, development, validation,

delivering you the complete biotechnological solution.

Show less

Not finding what you're looking for?

Get info on this provider's capabilities without requesting a quote.
FJ

Pure Biologics S.A. has not received any reviews.

Pure Biologics S.A. has not received any endorsements.