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Pure Biologics, Ltd.

Wrocław, PL

Pure Biologics is a specialized CMO&CRO with expertise on protein engineering and efficient production of difficult to express proteins, as well as methods for the selection of recombinant antibodies through Phage Display, for biomedical and diagnostic use. The company’s technologies are supported by extensive R&D activities and the unique in-house expertise and know-how of the company.

We have a good track record of successful generation and formulation of recombinant proteins and antibodies at their discovery stage (PureFold™, PurePerformance™) as drug candidates for preclinical studies, antigens and research tools. Together with detailed biophysical characterization of biologics and custom assay development abilities, we serve our numerous collaborators not only as a service provider, but primarily as a professional think tank and R&D partner solving complex protein related... Show more »

Pure Biologics is a specialized CMO&CRO with expertise on protein engineering and efficient production of difficult to express proteins, as well as methods for the selection of recombinant antibodies through Phage Display, for biomedical and diagnostic use. The company’s technologies are supported by extensive R&D activities and the unique in-house expertise and know-how of the company.

We have a good track record of successful generation and formulation of recombinant proteins and antibodies at their discovery stage (PureFold™, PurePerformance™) as drug candidates for preclinical studies, antigens and research tools. Together with detailed biophysical characterization of biologics and custom assay development abilities, we serve our numerous collaborators not only as a service provider, but primarily as a professional think tank and R&D partner solving complex protein related problems.

Commitment:
• Dedicated project manager for each client with 24/7 client support;
• On-time delivery with the highest quality;
• ISO 17025 and cGLPcompliant;
• State-of-the-art platforms and facilities;
• Clients’ confidentiality maintained at highest level;
• Backed by Laboratory Information Management System (LIMS);
• Online reporting available for clients;
• Logistics support.

www.PureBiologics.com

Pure Biologics Ltd, Dunska 11 street, 54-427 Wroclaw, Poland
Pure Biologics Branch in Berlin, Rudower Chaussee 29, 12489 Berlin, Germany

Poland: +48 570 00 28 29
Germany: +49 30 374 334 69
USA: +1 (415) 830 63 41

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Pure Biologics, Ltd. has not listed any services.

Antigen Expression and Purification
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Phage Display Library Construction and Screening
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Antibody Screening
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Protein Engineering
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Recombinant Protein Expression
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Protein production and purification (preclinical grade up to 10 g, >98% purity, up to 10 mg/ml)

The company provides a complete gene-to-protein service:

  1. Genetic engineering:
    • Custom cloning and mutagenesis;
    • Custom vectors and constructs;
    • Protein construct optimization.

  2. Protein... Show more »

Protein production and purification (preclinical grade up to 10 g, >98% purity, up to 10 mg/ml)

The company provides a complete gene-to-protein service:

  1. Genetic engineering:
    • Custom cloning and mutagenesis;
    • Custom vectors and constructs;
    • Protein construct optimization.

  2. Protein production:
    • Biofermentation capacity:
    (i) bioreactors and shakers (2, 10, 40, 120 liters),
    (ii) batch, fed-batch and continuous modes.
    • Expression platforms:
    (i) E. coli, Pichia and Mammalian systems,
    (ii) large collection of expression hosts and vectors.

  3. Protein purification:
    • Purity specified by client (affinity, IEX, IMAC, HIC, SEC, RP-HPLC).

  4. Bioanalysis report for each protein delivered:
    • Quality control and assurance specified by client (QA/QC):
    (i) purity and homogenity (PAGE, western blot, MS analyses),
    (ii) identity (MS/MS, N- & C- term sequencing),
    (iii) aggregation/oligomerization (DLS, CD, SEC),
    (iv) structural analysis (CD),
    (v) activity (ELISA, SPR, MTT, FACS, UV-Vis),
    (vi) stability studies (DSC, temperature scans CD, fluorescence),
    (vii) protein-protein or protein-ligand interaction studies.

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Bacterial Protein Expression
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Protein Expression in Mammalian Cells
Mammalian cell based recombinant protein production
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Protein Purification
Price on request

>98% purity

• French press or sonication disintegration;
• Affinity, IEX, IMAC, HIC, SEC, RP-HPLC;
• Tens of resins, high-throughput screening;
• Non-tagged, two step purification favored;
• Proprietary refolding screens for IB renaturation;
• Nucleic acid and pyrogenremoval;
• Constant activity and purity... Show more »

>98% purity

• French press or sonication disintegration;
• Affinity, IEX, IMAC, HIC, SEC, RP-HPLC;
• Tens of resins, high-throughput screening;
• Non-tagged, two step purification favored;
• Proprietary refolding screens for IB renaturation;
• Nucleic acid and pyrogenremoval;
• Constant activity and purity monitoring;
• Extensive formulation screens towards high mg/ml count and storage;
• Up to grams quantities.

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Protein Refolding
Price on request

HTS platform to refold proteins:
• Proprietary HT protein refolding screens;
• Two hundred protein refolding conditions evaluated;
• UV and CD monitoring process;
• Fine adjustment scouting for best performers and scale up;
• Structure and bioactivity assessment of renatured proteins;
• Custom protein refolding screens... Show more »

HTS platform to refold proteins:
• Proprietary HT protein refolding screens;
• Two hundred protein refolding conditions evaluated;
• UV and CD monitoring process;
• Fine adjustment scouting for best performers and scale up;
• Structure and bioactivity assessment of renatured proteins;
• Custom protein refolding screens available for different classes of protein.

Potential applications:
• Toxic proteins;
• Produce active proteins which cannot be expressed in conventional eukaryotic systems due to toxicity;
• Improved yield of biologically active protein;
• Overexpress protein in insoluble form which lack native structure and biological activity.

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Antibody Discovery
Price on request

Recombinant antibody discovery platform:
• Custom high-throughput screening campaign (Phage Display);
• Utilize clients’ specified assays and criteria;
• Differential selection to discriminate between antigens;
• Specific and tight binders delivered;
• Bi-specific antibodies.

Feasible antigens:
• Toxic antigens;
• Non-immunogenic antigens;
• Whole cells if biomarker not known.

Recombinant antibody discovery platform:
• Custom high-throughput screening campaign (Phage Display);
• Utilize clients’ specified assays and criteria;
• Differential selection to discriminate between antigens;
• Specific and tight binders delivered;
• Bi-specific antibodies.

Feasible antigens:
• Toxic antigens;
• Non-immunogenic antigens;
• Whole cells if biomarker not known.

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Antibody Engineering Services
Price on request

• Custom conjugations including
• Antibody Drug Conjugation (ADC)
• PEGs
• Fluorescent labels

• Custom conjugations including
• Antibody Drug Conjugation (ADC)
• PEGs
• Fluorescent labels

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Recombinant antibody production
Price on request

• Up to gram quantities
• Preclinical grade (up to 10g, >98% purity, 10mg/ml)

• Up to gram quantities
• Preclinical grade (up to 10g, >98% purity, 10mg/ml)

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Custom Cell-Based Assays
Price on request

Comprehensive assay development services based on clients’ specifications and needs.

Kinetics and biophysics of protein-protein, protein-ligand & DNA interactions.

In-house capabilities include:
• Surface plasmon resonance (SPR) analysis:
(i) kinetics of interaction (Kd, kon, koff),
(ii) affinity and avidity... Show more »

Comprehensive assay development services based on clients’ specifications and needs.

Kinetics and biophysics of protein-protein, protein-ligand & DNA interactions.

In-house capabilities include:
• Surface plasmon resonance (SPR) analysis:
(i) kinetics of interaction (Kd, kon, koff),
(ii) affinity and avidity assessments in high throughput mode;

• Isothermal Titration Calorimetry (ITC) analysis:
(i) mechanism of interaction (Kd, DH, DS, stoichiometry);

• ELISA:
(i) assay development and validation,
(ii) custom recombinant antibody generation by phage display if necessary;

• Fluorescence intensity and polarization:
(i) kinetics of interaction (Kd),
(ii) fluorescent probe assay suitability assessment;

• Enzyme kinetics parameterization (kcat, Vmax, Km, IC50, Ki)

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Bioanalytical Assays
Price on request

Full suite of protein bioanalytics for quality assurance tailored to clients’ needs:

• Mass spectrometry (MS) analyses:
(i) electrospray ionization (ESI), matrix-assisted laser desorption/ionization (MALDI) and tandem MS,
(ii) detect and identify a wide range of post-translational and chemical modifications;

• Biacore... Show more »

Full suite of protein bioanalytics for quality assurance tailored to clients’ needs:

• Mass spectrometry (MS) analyses:
(i) electrospray ionization (ESI), matrix-assisted laser desorption/ionization (MALDI) and tandem MS,
(ii) detect and identify a wide range of post-translational and chemical modifications;

• Biacore surface plasmon resonance (SPR);

• Dynamic Light Scattering (DLS);

• Size-Exclusion Chromatography (SEC);

• Thermodynamic stability determinations:
(i) circular Dichroism (CD), fluorescence and differential scanning calorimetry (DSC);
• Fluorescence activated cell sorting (FACS);

• Ultraviolet-visible spectroscopy (UV-Vis);

• Enzyme-linked immunosorbent assay (ELISA).

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Stable Cell Line Generation
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Mammalian Cell Culture
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Therapeutic Monoclonal Antibody (mAb) Development
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Bioanalysis
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Protein Services
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Biochemistry & Molecular Biology
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Antibody Services
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Biology
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Protein Expression
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Protein Purification and Quantification
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Cell-Based Assays
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Cells and Tissues
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Specialized Cell-Based Assays
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Pharmacology & Toxicology
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