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Purdue Proteomics Facility

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West Lafayette, Indiana, US

About Purdue Proteomics Facility

The Purdue Proteomics Facility (PPF) provides innovative state-of-the-art LCMS/MS analysis of proteins in clinical, environmental and other biological samples. As a shared facility of the Bindley Bioscience Center (BBC) in Discovery Park of Purdue University, the facility enables both targeted and global analysis of proteins, their post-translational modifications and analysis of protein complexes and protein-protein interactions, using both intact protein (‘top-down’) and peptide fragment (‘bottom-up’) analyses.  Coupled with different chromatographic separation techniques, modern mass spectrometric instrumentation, and advanced scientific and bioinformatics expertise, the facility provides unique opportunities for researchers at Purdue and beyond to perform quantitative analysis of  proteins in complex biological samples using both global, as well as targeted proteomic approaches.

Our Services (14)


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Biomarker Discovery

Price on request
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LTQ-MS

LTQ Linear Ion Trap Mass Spectrometry
Starting at $76.00 per hour

The Orbitrap is a sub-dalton-resolution proprietary ion trap configuration instrument that enables very high resolution analysis of peptides, post-translationally modified peptides as well as small molecule detection and quantification. The Orbitrap rivals FT-ICR mass spectrometers with an easy to use and easy to maintain setup.


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MALDI TOF-TOF MS

Matrix-assisted laser desorption ionization time-of-flight tandem mass spectrometry
Starting at $71.00 per hour

The advantages of MALDI-TOF TOF include sensitivity into the picomole range and high mass accuracy. In addition, since there is usually only a singly charged ion formed, it is a good choice for the analysis of heterogeneous samples. Furthermore, the static nature of the sample allows for multiple evaluations on a single sample.


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Nano-LC-ITMS

Nano Liquid Chromatography Coupled Ion Trap Mass Spectrometry
Starting at $48.00 per hour

The advantage of the ESI TOF is that it utilizes both the capacity of ESI to generate multiple charge states and extend the mass range of the spectrometer by reducing the m/z ratio of large ions and the high resolution and mass acuracy afforded the TOF instruments. This is particluarly useful when preforming PMF studies with complexed mixtures. Furthermore, the Agilent MSD TOF can be coupled to the Chip cube.

Agilent MSD

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Nano-LC-QQQ-MS

Nano Liquid Chromatography Coupled Triple Quadrupole Mass Spectrometry
Starting at $62.00 per hour

The Agilent 6410 triple quad provides high level sensitivity for selected ion monitoring and multiple reaction monitoring for biomarker quantification studies. The 6410 includes both ESI and photoionization capability and has an on-board Chip Cube nanoLC system.

Agilent 6410

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Bioanalysis

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Pharmacology & Toxicology

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Mass Spectrometry

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Imaging & Spectroscopy

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Biomarkers

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MALDI Mass Spectrometry Methods

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Spectroscopy

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Tandem Mass Spectrometry Methods

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Nano Mass Spectrometry Methods

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