Qfpweo5ctligibhqe9ek provitro

provitro AG

Berlin, DE

rovitro stand for more than ten years of experience in commercialisation of cell culture technologies and tissue microarrays backed up by scientific knowledge and resources of immunohistochemical analyses at the Pathological Institute of Charité Universitätsmedizin Berlin. We have added medical research to our own know-how in biotechnology to create solutions to common problems in life sciences. Provitro’s team has raised its sights to the interface between research and development to hammer out brand-new solutions to the benefit of future research projects. These are the hallmarks: competence, vision and sustainability.

Based on our quality management system certified according to ISO 9001 and ISO 13485, we are prepared to provide our customers not only with high-quality products and state-of-the-art services but also with individual solutions for their specific needs.

provitro AG has not listed any services.

Human Tissue Procurement
Price on request

Provitro’s tissue bank holds more than 1,000,000 samples which have been classified clinically and histopathologically. According to the customer’s specifications, tissues are collected and assembled
to TMA by our pathologists. Our experienced pathologists analyse the expression profile and provide you with a summary report, and... Show more »

Provitro’s tissue bank holds more than 1,000,000 samples which have been classified clinically and histopathologically. According to the customer’s specifications, tissues are collected and assembled
to TMA by our pathologists. Our experienced pathologists analyse the expression profile and provide you with a summary report, and stained tissue array slides along with representative digital images.

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DNA/RNA Analysis and Quality Control
Price on request
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Agilent 2100 Bioanalyzer
Immunohistochemistry (IHC)
Price on request

Immunohistochemical staining
Identification of antisera, immunoglobulin fractions and monoclonal antibodies to a growing number of clinically relevant tissue antigens has led to an enormous enlargement of immunohistochemical analyses in both quality and quantity. Antibody title and dilution as well as incubation time and... Show more »

Immunohistochemical staining
Identification of antisera, immunoglobulin fractions and monoclonal antibodies to a growing number of clinically relevant tissue antigens has led to an enormous enlargement of immunohistochemical analyses in both quality and quantity. Antibody title and dilution as well as incubation time and temperature are closely linked to each other with regard to their influence on the quality of immunohistochemical staining. These factors are varied either separately of each other or each of them in itself to achieve clearly recordable differences in staining quality. Parameters are varied primarily for the purpose of accomplishing staining of optimum specificity against minimum background.
We perform automated staining om various platforms, e.g. Benchmark XT, Bond maX

Standard histological staining
The morphological structure of tissue can be depicted with high accuracy by means of histological sections. Staining results depend on numerous factors, including pH of the buffer solution, staining time and method of fixation. Haematoxylin, for example, is used to stain cell and tissue structures, such as cell nuclei, mitochondria,
myelin, elastin and collagen fibres. Additional information may be obtained from counterstaining (differential staining), using a dyestuff in high contrast to hematoxylin stain. Counterstaining, using eosin, is a classical approach for which cationic structures are stained (e.g. proteins).

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VENTANA BenchMark XT
KingFisher FLEX
QIA Xtractor
KingFisher mL
Biobanking
Price on request

Provitro’s tissue bank holds more than 1,000,000 samples which have been classified clinically and histopathologically. According to the customer’s specifications, tissues are collected and assembled
to TMA by our pathologists. Our experienced pathologists analyse the expression profile and provide you with a summary report, and... Show more »

Provitro’s tissue bank holds more than 1,000,000 samples which have been classified clinically and histopathologically. According to the customer’s specifications, tissues are collected and assembled
to TMA by our pathologists. Our experienced pathologists analyse the expression profile and provide you with a summary report, and stained tissue array slides along with representative digital images.

« Show less
VENTANA BenchMark XT
KingFisher FLEX
QIA Xtractor
KingFisher mL
Tissue Microarrays
Price on request

Tissue microarrays are an ideal tool in high-throughput analysis for antigen distribution or validation of newly identified pathogenetic genes. Tissue microarrays with up to 100 individual tissue samples on a single slide minimise variations in staining intensity, yielding consistent results. Tissue spots with a diameter of up to... Show more »

Tissue microarrays are an ideal tool in high-throughput analysis for antigen distribution or validation of newly identified pathogenetic genes. Tissue microarrays with up to 100 individual tissue samples on a single slide minimise variations in staining intensity, yielding consistent results. Tissue spots with a diameter of up to 3 mm provide sufficient area for morphological evaluation. Tissue microarrays provide a rapid solution for localisation of DNA or protein molecules in normal and pathological human tissues.

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VENTANA BenchMark XT
KingFisher FLEX
QIA Xtractor
KingFisher mL
RNA Extraction
Price on request

Provitro’s quality management certified lab offers services for total RNA isolation from cells and various tissue types. Total RNA is isolated from your material, using the QIAGEN RNeasyTM system. Purified RNA will be aliquoted as necessary for further investigation according to the customer’s need and will be stored clearly... Show more »

Provitro’s quality management certified lab offers services for total RNA isolation from cells and various tissue types. Total RNA is isolated from your material, using the QIAGEN RNeasyTM system. Purified RNA will be aliquoted as necessary for further investigation according to the customer’s need and will be stored clearly labelled at -80°C. All processes are accomplished according to standard operation procedures (SOP). Quality control and product release are arranged according to clearly defined criteria.

Standard quality control

Standard quality control is run for the purpose of checking on the ratio between absorbance values at 260 and 280 nm. That ratio provides for an estimate of the purity of RNA with regard to contaminants absorbed in the UV, such as proteins. The results allow to quantify the total amount of purified RNA and reveal possible sample contaminations detectable in the UV-range.

Advanced quality control

On request, each sample may be tested regarding RNA degradation or signs of apoptose, using the Agilent 2100 Bioanalyser System. The resulting electropherogramme is checked for baseline development, marker peak and ribosomal signal intensity (ratio 28S/18S RNA).

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VENTANA BenchMark XT
KingFisher FLEX
QIA Xtractor
KingFisher mL
Nucleic Acid Hybridization
Price on request

Chip hybridisation is done in cooperation with a highly experienced local partner. In addition to standard hybridisation of Affymetrix arrays, this facility is furnished with a competent and experienced lab specialised in the development and hybridisation of customised arrays. For chip hybridisation, 5.0 μg RNA (5 μg/10 μl) of your sample will be passed directly to our local partner.

Chip hybridisation is done in cooperation with a highly experienced local partner. In addition to standard hybridisation of Affymetrix arrays, this facility is furnished with a competent and experienced lab specialised in the development and hybridisation of customised arrays. For chip hybridisation, 5.0 μg RNA (5 μg/10 μl) of your sample will be passed directly to our local partner.

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VENTANA BenchMark XT
KingFisher FLEX
QIA Xtractor
KingFisher mL
Sanger Sequencing
Price on request

Molecular diagnostics categorises cancer using technologies such as mass spectrometry and sequencing, hereby determining how genes and proteins are interacting in a cell. The aim is to disclose modified expression patterns – gene and protein activity patterns – in different types of cancerous or praecancerous cells. These sets of... Show more »

Molecular diagnostics categorises cancer using technologies such as mass spectrometry and sequencing, hereby determining how genes and proteins are interacting in a cell. The aim is to disclose modified expression patterns – gene and protein activity patterns – in different types of cancerous or praecancerous cells. These sets of changes, also called »molecular signatures«, are improving the clinicians’ ability to diagnose cancer, and provide an individualized therapy to the patient.

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VENTANA BenchMark XT
KingFisher FLEX
QIA Xtractor
KingFisher mL
Custom Cell Culture Media
Price on request

For more then ten years, provitro have been working for various scientists who need flexibility up to the composition of the basal medium. Therefore, we offer our customer service. You tell us the modifications needed, and we produce five or more units of your personal cell growth medium. Ask for detailed terms of conditions for this service.

For more then ten years, provitro have been working for various scientists who need flexibility up to the composition of the basal medium. Therefore, we offer our customer service. You tell us the modifications needed, and we produce five or more units of your personal cell growth medium. Ask for detailed terms of conditions for this service.

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VENTANA BenchMark XT
KingFisher FLEX
QIA Xtractor
KingFisher mL
Primary Cell Isolation
Price on request

Each unit (vital = proliferating flask, or cryo = cryopreserved) contains more than 500,000 cells. Each cell strain is performance tested by extensive growth assays and inspection of cell morphology. Provitro guarantees a minimum of 10 population doublings of most normal human cell culture. Cell tests are negative for HIV-1 DNA,... Show more »

Each unit (vital = proliferating flask, or cryo = cryopreserved) contains more than 500,000 cells. Each cell strain is performance tested by extensive growth assays and inspection of cell morphology. Provitro guarantees a minimum of 10 population doublings of most normal human cell culture. Cell tests are negative for HIV-1 DNA, hepatitis B DNA, hepatitis C DNA, mycoplasma, bacteria, yeast and fungi. Provitro guarantees the homogeneity of each cell type according to cell type-specific test markers. Each unit comes with a certificate specifying the
results of quality control for this cell culture.

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VENTANA BenchMark XT
KingFisher FLEX
QIA Xtractor
KingFisher mL
Cytotoxicity Assays
Price on request

Cell culture test of new/toxic substances on primary cells and defined cell lines, respectively

Cytotoxicity testing is based on the general assumption that any substance, depending on its concentration, may have toxic effects on human cells. The test concerned will define and identify basal cytotoxicity, independent of... Show more »

Cell culture test of new/toxic substances on primary cells and defined cell lines, respectively

Cytotoxicity testing is based on the general assumption that any substance, depending on its concentration, may have toxic effects on human cells. The test concerned will define and identify basal cytotoxicity, independent of metabolic effects. The test system may be used to check all aqueous or water-soluble samples as well as eluates of solids for their toxicity on human cells.

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VENTANA BenchMark XT
KingFisher FLEX
QIA Xtractor
KingFisher mL
DNA Sequencing
Price on request
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Protein Expression Visualization
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Protein Services
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Biology
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Immunostaining
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Cells and Tissues
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Biospecimens
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Toxicology
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Primary Cell Culture
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Human Biospecimen Collection
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Human Biospecimen Management and Storage
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DNA
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RNA
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Pharmacology & Toxicology
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Cell Culture Media and Reagents
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Biochemistry & Molecular Biology
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Nucleic Acid Services
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DNA Hybridization
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