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ProtTech, Inc.

2 Orders Completed
Oaks, Pennsylvania, US

About ProtTech, Inc.

Type: Partnership Size: 11-50 employees

ProtTech Inc., with cutting edge protein technologies it developed over a decade, provides high quality and high valued services for biological research community in protein identification, protein characterization, antibody de novo sequencing, protein mutation analysis, protein modification analysis, proteomics, therapeutic protein comparability studies, etc. For NeoAntigen or immunopeptidomic study, we provide creative LC-MS/MS based solutions. For biologic discovery and development, we provide contract research services in novel antibody drug discovery based our proprietary IDAS technology platform, which is capable to directly explore the antibody repertoire in animal and human serum to discover antibody therapeutics with high affinity and naturally optimized sequences. For bio-similar development, we provide contact services including the sequencing of original marketed products, cell line development and comparability studies. We also provide the complete technology platform for the development of antibody fragments such as F(ab’)2 and Fab, which have wide uses as therapeutic, diagnostic and image agents. 

Diversity Certificates

Small business

Our Services (17)


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Metabolite Identification

Price on request

Identification of metabolites from both in vivo and in vitro samples. Depending on the demand from customer, we provide two type of services: 

1. Top 10 metabolites (by MS intensity) for quick scanning of each compound.

2. Complete profiling of metabolites.




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Neoantigen Identification

Price on request

ProtTech has developed a platform to identify neoantigens by sequencing MHC I and/or MHC II bound peptides by biochemical isolation, e,g, the immunoprecipitation of human MHC I-peptide complex by W6/32 antibodies. The isolated peptides are analyzed by an ultra-sensitive NanoLC-MS/MS. This platform, known as NeoMS, is a proprietary software suite dedicated to the sequencing and analysis of MHC class I and class II antigens, particularly neoantigens presented by tumor cells and antigen-presenting cells (APC). The functionality of the software suite includes:

1). Identifying tumor neoantigens derived from somatic mutations, such as amino acid substitutions, deletion, insertion, reading frame shift in translation, etc;

2). Identifying tumor neoantigens arrived from abnormal expression of the germ cell specific proteins (cancer testis antigens);

3). Identifying intron retention neoantigens.


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Antibody Fragment Production

Price on request

We provide a service package making antibody fragments (Fab2 or Fab) from full-length monoclonal antibodies. Our technique platform allows > 90% conversion from full-length antibody to high purity antibody fragments. Please contact us for your protein analysis inquiry or request.

Application of antibody fragments

  1. As protein drugs
  • As diagnostic and image agents
  • As part of antibody-drug conjugates


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Biopharmaceutical Development

Price on request

We provide following service package to help our clients in the development of bio-similar antibodies. It contain following steps:

  1. Determining the exact sequences of a marketed antibody drug product .
  • Developing CHO cell lines for antibody expression.
  • Expression and purification of antibody in small amount.
  • Conducting comparability study of expressed antibody with the marketed antibody drug as reference.


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Mutation Analysis

Price on request

There are two major applications for protein mutations analysis: 1). To find out the exact amino acid sequence of a commercially available protein product in which its amino acid sequence has been altered to give better or new properties; 2). Screening unwanted mutations at the protein level in clone selection process before the production of a protein therapeutics.

Many high commercial valued proteins, such as proteins used in industrial processes, contain amino acid sequences different from their native sequences, which give them superior properties than that of the native proteins. Since the exact amino acid sequence of such a modified protein is often non-disclosed, but the protein product is widely available, there is often a great interest in determining the exact amino acid sequences of those protein products. To meet the demand, we have developed analytic procedures and bioinformatics tools that can accurately and completely determine all mutations in protein products, even in the case that protein is heavily mutated throughout its entire sequence that gives it’s a new catalytic activity.

There are also needs to detect unwanted sequence changes in proteins. It becomes clear now that spontaneous mutations (also called sequence variant, SV) occur during cell line generation and storage. A minor amount of SV in a production clone may lead to a high ratio of the final product. Since the sequence variants often have altered bioactivity and higher immunogenicity and are very difficult to remove, the presence of an even low level of a sequence variant in biomanufactory product may lead to a commercial disaster. Consequently, screening for unwanted mutations at protein level before large scale production is becoming a requirement although detecting SV is a difficult analytical task. To help our clients meet this challenge, we have developed the technique that is capable of detecting SV as low as 0.01% and our screening cover the entire mAb/protein sequence, and we have carried out SV screening or several dozen therapeutic products for global pharmaceutical companies.


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Proteomics

Price on request

Proteomic analysis is an essential tool in the discovery of protein biomarkers, which may act as targets for disease diagnostics and therapeutic interventions. ProtTech provides LC-MS based proteomic analysis. Building on our broad range of expertise in protein analysis and protein purification and functional studies, we are dedicated to deliver a high valued service package to our clients.

We have two main platforms in the proteomic analysis: label-free LC-MS based proteomics and SILIC LC-MS based proteomics. With our expertise in protein purification, protein immobilization protein chemistry and proteomic sample processing, we also provide add-on services to meet our clients’ various needs, including sample isolation, protein purification, chemical labeling, immobilization on a solid phase, pathway analysis, etc. Our integrated services will help our clients in the study of phosphoproteomics. Chemoproteomics, organelle proteomics, signaling proteomics and other research areas.


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N-Terminal Sequencing

Price on request

Due to in vivo processing and post-translational modifications, the N- and C-terminus of a protein are not predictable based on its gene sequence, and heterogeneous or chemically modified N- and C-terminus can be present in protein product, including antibodies, which is often difficult for Edman degradation based analysis.

ProtTech provides a service for characterizing the N- and C-terminus of any protein or antibody with a known gene sequence. Based on LC-MS technique, we are capable of identifying both protein N- and C-terminus, to identify heterogeneous N- or C-terminal, and to identify chemically modified protein N- or C-terminal. Please contact us if you are interest in this service.

Applications:

  1. Unknown protein N- or C- terminal
  • Proteins with capped N-terminal
  • Proteins with multiple N- or C-terminals
  • N- or C-terminal in protein mixtures
  • New N- or C-terminal resulted from proteolytic cleavage or degradation.

Technical Advantage

Our N-and C-terminal identification service is based on multiple technologies we have developed, such as isotope labeling of protein N- and C-terminus, bioinformatics software for the analysis of protein N- and C-terminus, multiple protein digestions for complete sequence coverage, and ultra-sensitive LC-MS technique.

Comparing to Edman N-terminal sequencing, our service provides the following advantages:

  1. Ability to identify protein C- terminal
  • Ability to identify proteins with capped N-terminal
  • Ability to identify proteins with multiple N- or C-terminus
  • Ability to identify N- or C- terminus in protein mixtures

Service Terms:

Sample Requirement: We typical need >20ug of protein with >80% purity for the analysis. Gel sample is acceptable, but a sample may need special treatments before running a gel.

Turnaround Time: Normally 2-weeks

Price: The price of N- and C-terminal analysis service will depend on protein size and its particular sequence, purity and available amount, as well as the specific requirements of our client. We’ll work out an optimal procedure for each project based on that information. Please send us your sample information and we’ll send you a formal quote within 12 hours. Also please contact us if you need sign confidentiality agreement with us before sending out your sample information.


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Protein Characterization

Price on request

Detailed analysis of protein structure is of vital importance in the study of protein functions and the development of biologics. Our Protein Characterization services offer in-depth analysis of the primary structure and disulfide bond linkage of purified or expressed proteins. Our Peptide Mapping service provides a high confident protein sequence conformation with 100% coverage. Our protein N-and C-terminal analysis can identify proteins with multiple or modified N- or C-terminals due to in vivo processing or proteolytic cleavage. Our disulfide bond mapping can analyze both correctly linked disulfide bonds as well mismatched disulfide bonds.


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Protein Identification

Price on request

Protein Identification (ProteinID) is an important technique in determining the identity of a protein and in studying the protein-protein interactions. We provide high values ProteinID services based on ultra-sensitive NanoLC-MS/MS, which can find the identity of an isolated protein sample in shorter time and at a lower cost than traditional Western Blot. We have served a large number of life science researchers around the world with our high quality ProteinID service and many high profile publications have been published related to our work.

To meet the requirement of different needs of our customers, we offer following three types of protein identification services.

  1. ProteinID for gel band/spots: Analyzing SDS-PAGE gel bands or 2D spot stained with coomassie, silver, fluorescent dye. [Details]
  • ProteinID for solution samples of the simple protein mixture: Up to 100 different proteins in a solution samples can be reliably identified by our high resolution and ultra-sensitive NanoLC-MS/MS procedure. [Details]
  • Complete profiling of a complex protein mixture: Up to 1000 different proteins in a complex protein mixture can be identified. [Details]


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C-Terminal Sequencing

Price on request

Due to in vivo processing and post-translational modifications, the N- and C-terminus of a protein are not predictable based on its gene sequence, and heterogeneous or chemically modified N- and C-terminus can be present in protein product, including antibodies, which is often difficult for Edman degradation based analysis.

ProtTech provides a service for characterizing the N- and C-terminus of any protein or antibody with a known gene sequence. Based on LC-MS technique, we are capable of identifying both protein N- and C-terminus, to identify heterogeneous N- or C-terminal, and to identify chemically modified protein N- or C-terminal. Please contact us if you are interest in this service.

Applications:

  1. Unknown protein N- or C- terminal
  • Proteins with capped N-terminal
  • Proteins with multiple N- or C-terminals
  • N- or C-terminal in protein mixtures
  • New N- or C-terminal resulted from proteolytic cleavage or degradation.

Technical Advantage

Our N-and C-terminal identification service is based on multiple technologies we have developed, such as isotope labeling of protein N- and C-terminus, bioinformatics software for the analysis of protein N- and C-terminus, multiple protein digestions for complete sequence coverage, and ultra-sensitive LC-MS technique.

Comparing to Edman N-terminal sequencing, our service provides the following advantages:

  1. Ability to identify protein C- terminal
  • Ability to identify proteins with capped N-terminal
  • Ability to identify proteins with multiple N- or C-terminus
  • Ability to identify N- or C- terminus in protein mixtures

Service Terms:

Sample Requirement: We typical need >20ug of protein with >80% purity for the analysis. Gel sample is acceptable, but a sample may need special treatments before running a gel.

Turnaround Time: Normally 2-weeks

Price: The price of N- and C-terminal analysis service will depend on protein size and its particular sequence, purity and available amount, as well as the specific requirements of our client. We’ll work out an optimal procedure for each project based on that information. Please send us your sample information and we’ll send you a formal quote within 12 hours. Also please contact us if you need sign confidentiality agreement with us before sending out your sample information.


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De Novo Antibody Sequencing

Price on request

ProtTech is providing one of the world best quality antibody de novo sequencing services. With as low as 100ug of a mAb protein, we can deliver a complete and accurate mAb protein sequences with distinguished leucine and isoleucine residues. We guarantee a 100% accuracy of delivered mAb sequences. If the expressed mAb with our delivered sequences has lower activity than the original mAb in ELISA assay, we’ll continue our effect to identify possible sequence errors free of charge.

Please contact us for your protein analysis inquiry or request.

Application of antibody de novo sequencing service:

The development of bio-similar antibody for diagnostic or therapeutic use.
The development of antibody drugs based on commercially available antibody reagents. Some commercially available antibody reagents may have neutralizing activity toward a drug target. Direct sequencing such a reagent antibody may offer a good starting point for further development such as affinity maturation and humanization.
Resolving ambiguity from mAb gene sequencing. Due to the higher error rate in RT-PCR and technical difficulty in mAb gene sequencing itself, it is not rare that the result from mAb gene sequencing has ambiguity. The mAb protein sequencing is a faster and cost-saving way to resolve sequence conflict.


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Therapeutic Monoclonal Antibody (mAb) Development

Price on request
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Antibody Services

Price on request
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Protein Sequencing

Price on request
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Protein Services

Price on request
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Peptide Mass Fingerprinting

Price on request
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LC-MS

Liquid Chromatography-Coupled Mass Spectrometry
Price on request
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KJ

Kerrie Jin

Manager

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