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Proteomics Shared Resource Georgetown Lombardi Comprehensive Cancer Center

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Washington, DC, US

About Proteomics Shared Resource Georgetown Lombardi Comprehensive Cancer Center

The mission of Georgetown University proteomics core is to accelerate discovery by giving investigators access to cutting edge technologies in mass spectrometry-based proteomics. By using advanced mass spectrometers and well-established proteomics technologies, we are devoted to help researchers to answer questions in basic, translational and clinical biomedical research.


Committed to providing researchers with high-quality, cost-effective services and support, we offer free consultation to each specific project, small- or large-scale, to ensure that expectations are properly managed and fulfilled. To keep scientists in our community competitive, new technologies will be incorporated into the facility as soon as possible to meet their needs. The facility is open to all investigators in and outside Georgetown University.


The high speed, sensitivity, and accuracy of our mass spectrometers allow accurate qualitative and quantitative measurements.


The most common experiments include:


1. Hypothesis-driven discovery proteomic studies of biological systems for the identification of peptides, proteins and post-translational modifications as well as their quantification by incorporating various strategies (e.g., iTRAQ-based stable isotopic labeling and label-free data independent quantification (including SWATH)


2. Targeted proteomics by mainly using multiple reaction monitoring (MRM) and parallel reaction monitoring (PRM)

Our Services (8)


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Mass Spectrometry

Price on request

The Proteomics Shared Resource, a part of Proteomics and Metabolomics Shared Resource at GUMC, is directed by Dr. Junfeng Ma. Our facility is equipped with multiple high-end mass spectrometers (e.g., Orbitrap Fusion Lumos, TripleTOF 6600 and QTRAP 6500) which can be coupled with nanoHPLC/UPLC systems (including nanoAcquity and Ultimate 3000).


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Molecular Weight Determination

Price on request

Molecular weight determination of protein/peptide


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Protein Identification

Price on request

- Protein identification from gel bands;

- Protein identification from complex mixtures.


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Post-Translational Modification Analysis

Price on request

Characterization of post-translational modifications (PTMs), including phosphorylation, acetylation, O-GlcNAcylation and other types of glycosylation.



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Protein Quantification

Price on request

Protein quantification: Isobaric tagging for relative and absolute quantitation (iTRAQ) and label-free quantification (including SWATH).


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Multiple Reaction Monitoring (MRM) Assay Panels

Price on request

Targeted quantification by Multiple Reaction Monitoring (MRM) and Parallel Reaction Monitoring (PRM).


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Proteomics

Price on request
Request a quote for more information about this service.

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Protein Characterization

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Junfeng Ma

Assistant Professor

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