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RNA Epitranscriptomics & Proteomics Resource

52 Orders Completed
Rensselaer, New York, US

About RNA Epitranscriptomics & Proteomics Resource

The RNA Epitrancriptomics & Proteomics Resource (REPR) provides solutions to the research community on a fee-for-service basis. These services are available to investigators at the University at Albany, other academic institutions, and commercial entities.

The REPR specializes in the identification of individual proteins, characterization of entire protein complexes or biosimilars, global analysis to discover changes in the proteomes of different samples, and RNA modifications. The laboratory also specializes in the development of custom assays for quantitation of specific molecules in complex clinical samples. The technologies above are available as core services. The REPR provides basic laboratory services to hundreds of bioscience researchers at companies, universities, and government agencies around the world, helping them create robust, reliable results quickly and affordably.

We handle projects of any size, from single sample analyses to long-term work as collaborators or subcontractors on NIH grants. Each client is assigned a Ph.D.-level scientist to coordinate their project, meeting budget and deadline needs.

Our Services (53)


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Quantitative Analysis of RNA Modifications

Price on request

Ultra-pressure liquid chromatography coupled with tandem mass spectrometry is used to analyze RNA modifications at different stages during cell differentiation or disease progression.There are about 112 modified nucleosides in our database. We can monitor about 40 nucleosides in a single LC-MS/MS run through Waters Xevo TQ-S system.

Oncology CNS/Neurology Genetic Disease UPLC LC-MS/MS HPLC lncRNA miRNA mRNA Neurodegenerative diseases Show 10 more tags Show less

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Intact Mass Analysis

Price on request

Analysis and characterization of intact mass of bio-molecules including protein, RNA, DNA and antibody using an optimized LC-MS workflow based on customized column coupled online with a ESI TOF mass spectrometer. Raw and deconvoluted spectra for the major peaks are provided.

Proteins Antibodies RNA DNA

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Protein Identification

Starting at $180.00 per sample
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Antibody Variant Analysis

Price on request

QTOF instrument is used to determine the intact mass of the antibody followed by sequencing the heavy and light chains of the antibody after deglycosylation. Multiple proteases are used in the procedure so as to obtain a "complete" sequencing coverage. Mascot and Proteinpilot mapping results are provided.
Deliverables: amino acid sequences of heavy and light chains; comprehensive report of experiments and analyses.

Antibodies Proteins Drug Discovery

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FPLC

Fast Performance liquid chromatography
Price on request
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Proteins DNA RNA Antibodies milligram

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HPLC

High Performance Liquid Chromatography
Starting at $35.00 per hour

Assessment of compound purity by LC-MS (HTQC)

Agilent Waters

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Protein Purification

Starting at $35.00 per hour

Let the Proteomics/Mass Spec group purify your protein using a purification protocol of your choice or one that we develop for you. Protein purification projects are typically completed in two-four weeks depending on services required. Chromatographic services include ion exchange, HIC, size exclusion, affinity tag and RP-HPLC. Standard QC assays include SDS-PAGE, western blot, and mass spectrometry.


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Protein AQUA

Price on request

Ultra sensitive quantitation of pg/ml level of protein with trap-and-elute micro-flow LC-MS/MS. The protein quantification is based on isotope dilution LC-MS/MS and Multiple or selective Reaction Monitoring (MRM, SRM) of specific peptides from the protein(s) of interest. This is a well-established approach for quantification of peptides and small molecules, and has now been adapted for absolute quantification of proteins in very complex mixtures.

AB Sciex QTRAP Shimadzu UHPLC MS/MS Detector

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Structure Determination

Starting at $100.00 per sample
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Impurity Analysis

Starting at $200.00 per sample

The combination of LC-UV-MS is used to assay the impurities of compound.

QTof

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High Resolution Mass Spectrometry

Starting at $85.00 per sample
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LC-MS

Liquid Chromatography-Coupled Mass Spectrometry
Starting at $100.00 per sample

Intact protein molecular weight - $100
Protein ID - $180


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Isothermal Titration Calorimetry (ITC)

Price on request

The MicroCal VP-ITC (Isothermal Titration Calorimeter) unit directly measures heat evolved or absorbed in liquid samples as a result of mixing precise amounts of reactants. A spinning syringe is utilized for injecting and mixing of reactants. Origin® software is used to analyze the ITC data using fitting models to calculate reaction stoichiometry (n), binding constant (KD), enthalpy (ΔH) and entropy (ΔS).

MicroCal VP-ITC

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MALDI TOF MS

Matrix-assisted laser desorption ionization time-of-flight mass spectrometry
Starting at $50.00 per sample
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PAGE

Polyacrylamide Gel Electrophoresis
Starting at $150.00 per sample

IPG Strip

SDS-PAGE Starting at $60/sample


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Targeted Mass Spectrometry (MRM/SRM)

Multiple Reaction Monitoring/Selected Reaction Monitoring Mass Spectrometry
Starting at $200.00 per run

We used a QTRAP 6500 mass spectrometer coupled with UHPLC for the analysis. The setup fee (parameter optimization) is $200 then $35 per sample injection.


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MudPIT Analysis

Multidimensional Protein Identification Technology
Starting at $100.00 per sample

per step


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Peptide Mapping

Starting at $75.00 per hour

de novo


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Protein Digestion

Starting at $100.00 per sample

Enzymatic Digest


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iTRAQ Protein Labeling

Isobaric Tags for Relative and Absolute Quantitation
Starting at $4,000.00 per sample

4 plex $4000 (> 25 fractions)
8 plex (> 25 fractions)
Phosphoprotein profiling using TiO2


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Phosphopeptide Mapping

Starting at $680.00 per sample

The service includes tryptic digest, TiO2 enrichment, LC-MS/MS analysis for flow through and enriched fraction, and Mascot sequencing mapping.


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MIDAS

Multiple Reaction Monitoring-Initiated Detection and Sequencing
Starting at $280.00 per sample

Targeted detection and quantitation, also called multiple reaction monitoring-initiated detection and sequencing (MIDAS). Multiple reaction monitoring (MRM) is used as an extremely sensitive MS survey scan for the targeted peptide from a known protein. This is automatically followed by peptide sequencing confirmation. The method is capable of detecting and sequencing peptides at low femtomole levels with high selectivity. The MIDAS workflow is extremely useful for biomarker validation and cellular analysis particularly when a monoclonal antibody is not available or western blot is inclusive. (Molecular &Cellular Proteomics 4:1134–1144, 2005).


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Gel Imaging

Starting at $40.00 per gel
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Gel Staining

Starting at $20.00 per gel

SYPRO Ruby staining

  • Use plastic tray as staining container
  • Rinse the gel 3 times with ddH2O
  • Fix the gel with 10% methanol, 7 % acetic acid, and 83% H2O for 60 min
  • Stain the gel in SYPRO Ruby solution which is at least 10 times the volume of the gel
  • Gently shake for at least 3 hour for maximal sensitivity or overnight
  • Wash the gel with 10% methanol, 7 % acetic acid, and 83% H2O for 30-60 minto decrease background fluorescence
  • Wash the gel 3 times - 5 min with ddH2O
  • Acquire gel image under a blue light transilluminator (image can be seen by hand-held UV source)
  • Store the gel in 1-2% acetic acid

Note that use SYPRO Ruby displays difficult to stain glycoproteins and lipoproteins


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Analytical Chemistry Services

Price on request
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Biochemistry & Molecular Biology

Price on request
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Specialty Mass Spectrometry Methods

Price on request
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Liquid Chromatography Coupled Mass Spectrometry Methods

Price on request
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Biology

Price on request
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Tandem Mass Spectrometry Methods

Price on request
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Omics

Price on request
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Electrophoresis

Price on request
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Protein Characterization

Price on request
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Other Mass Spectrometry Methods

Price on request
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Calorimetry

Price on request
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Spectroscopy

Price on request
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Physical Analysis Methods

Price on request
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Nucleic Acid Services

Price on request
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Chromatography

Price on request
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MALDI Mass Spectrometry Methods

Price on request
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Mass Spectrometry

Price on request
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Protein Sequencing

Price on request
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Separation/Purification Services

Price on request
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Antibody Services

Price on request
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Liquid Chromatography (LC)

Price on request
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Imaging & Spectroscopy

Price on request
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Nucleic Acid Analysis

Price on request
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Protein Services

Price on request
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Proteomics

Price on request
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Chemistry and Materials

Price on request
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Gel Electrophoresis

Price on request
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Protein Quantification

Price on request
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Ion Exchange Chromatography

Price on request
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Qishan Lin, PhD

Project Administrative Officer

FileType/PDF Created with Sketch. Antibody sequencing assembly.pdf

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FileType/PDF Created with Sketch. Example of Certification of Aanalysis.pdf

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FileType/PDF Created with Sketch. Gel sample instruction.pdf

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FileType/PDF Created with Sketch. Quantifying 36 modifications in RNA.pdf

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FileType/PDF Created with Sketch. UAMassBR2-2018A.pdf

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  • Positive review for LC-MS:

    May 2017

    "Dr Lin analyzed and produced the results within 3 days of receiving the sample. His price was competitive and was quick to respond to several questions. We definitely recommend this lab."

  • Positive review for MALDI TOF MS:

    November 2016

    "We have just completed our fist project with Science Exchange Service and Proteomics/Mass from Univ. of Albany. We are completely satisfied! Great support, fast turn-around results, expertise in analysis recommendation and execution. We definitely recommend, and will work again with Proteomics group again. Michael Quintero, MS SMG Prod. Manager/Research Scientist King Industries, Inc."

  • Positive review for HPLC:

    October 2015

    "Absolutely excellent and professional service, friendly staff, will definitely use again."

  • Positive review for Peptide Mapping:

    August 2015

    "Quick response, excellent work, very satisfied."

  • Positive review for High Resolution Mass Spectrometry:

    May 2015

    "Qishan is extremely prompt and helpful."

  • Positive review for High Resolution Mass Spectrometry:

    April 2015

    "Qishan is extremely prompt and helpful."

  • Positive review for HPLC:

    July 2014

    "Machine was down but he pulled through anyways"

  • Positive review for MALDI TOF MS:

    November 2012

    "Excellent service all around!"

  • Positive review for Mass Spectrometry:

    August 2012

    "Excellent service and turnaround time"

RNA Epitranscriptomics & Proteomics Resource has not received any endorsements.