The RNA Epitrancriptomics & Proteomics Resource (REPR) provides solutions to the research community on a fee-for-service basis. These services are available to investigators at the University at Albany, other academic institutions, and commercial entities.
The REPR specializes in the identification of individual proteins, characterization of entire protein complexes or biosimilars, global analysis to discover changes in the proteomes of different samples, and RNA modifications. The laboratory also specializes in the development of custom assays for quantitation of specific molecules in complex clinical samples. The technologies above are available as core services. The REPR provides basic laboratory services to hundreds of bioscience researchers at companies, universities, and government agencies around the world, helping them create robust, reliable results quickly and affordably.
We handle projects of any size, from single sample analyses to long-term work as collaborators or subcontractors on NIH grants. Each client is assigned a Ph.D.-level scientist to coordinate their project, meeting budget and deadline needs.
Ultra-pressure liquid chromatography coupled with tandem mass spectrometry is used to analyze RNA modifications at different stages during cell differentiation or disease progression.There are about 112 modified nucleosides in our database. We can monitor about 40 nucleosides in a single LC-MS/MS run through Waters Xevo TQ-S system.
Analysis and characterization of intact mass of bio-molecules including protein, RNA, DNA and antibody using an optimized LC-MS workflow based on customized column coupled online with a ESI TOF mass spectrometer. Raw and deconvoluted spectra for the major peaks are provided.
QTOF instrument is used to determine the intact mass of the antibody followed by sequencing the heavy and light chains of the antibody after deglycosylation. Multiple proteases are used in the procedure so as to obtain a "complete" sequencing coverage. Mascot and Proteinpilot mapping results are provided.
Deliverables: amino acid sequences of heavy and light chains; comprehensive report of experiments and analyses.
Assessment of compound purity by LC-MS (HTQC)
Let the Proteomics/Mass Spec group purify your protein using a purification protocol of your choice or one that we develop for you. Protein purification projects are typically completed in two-four weeks depending on services required. Chromatographic services include ion exchange, HIC, size exclusion, affinity tag and RP-HPLC. Standard QC assays include SDS-PAGE, western blot, and mass spectrometry.
Ultra sensitive quantitation of pg/ml level of protein with trap-and-elute micro-flow LC-MS/MS. The protein quantification is based on isotope dilution LC-MS/MS and Multiple or selective Reaction Monitoring (MRM, SRM) of specific peptides from the protein(s) of interest. This is a well-established approach for quantification of peptides and small molecules, and has now been adapted for absolute quantification of proteins in very complex mixtures.
The combination of LC-UV-MS is used to assay the impurities of compound.
Intact protein molecular weight - $100
Protein ID - $180
The MicroCal VP-ITC (Isothermal Titration Calorimeter) unit directly measures heat evolved or absorbed in liquid samples as a result of mixing precise amounts of reactants. A spinning syringe is utilized for injecting and mixing of reactants. Origin® software is used to analyze the ITC data using fitting models to calculate reaction stoichiometry (n), binding constant (KD), enthalpy (ΔH) and entropy (ΔS).
SDS-PAGE Starting at $60/sample
We used a QTRAP 6500 mass spectrometer coupled with UHPLC for the analysis. The setup fee (parameter optimization) is $200 then $35 per sample injection.
4 plex $4000 (> 25 fractions)
8 plex (> 25 fractions)
Phosphoprotein profiling using TiO2
The service includes tryptic digest, TiO2 enrichment, LC-MS/MS analysis for flow through and enriched fraction, and Mascot sequencing mapping.
Targeted detection and quantitation, also called multiple reaction monitoring-initiated detection and sequencing (MIDAS). Multiple reaction monitoring (MRM) is used as an extremely sensitive MS survey scan for the targeted peptide from a known protein. This is automatically followed by peptide sequencing confirmation. The method is capable of detecting and sequencing peptides at low femtomole levels with high selectivity. The MIDAS workflow is extremely useful for biomarker validation and cellular analysis particularly when a monoclonal antibody is not available or western blot is inclusive. (Molecular &Cellular Proteomics 4:1134–1144, 2005).
SYPRO Ruby staining
Note that use SYPRO Ruby displays difficult to stain glycoproteins and lipoproteins
"Dr Lin analyzed and produced the results within 3 days of receiving the sample. His price was competitive and was quick to respond to several questions. We definitely recommend this lab."
"We have just completed our fist project with Science Exchange Service and Proteomics/Mass from Univ. of Albany. We are completely satisfied! Great support, fast turn-around results, expertise in analysis recommendation and execution. We definitely recommend, and will work again with Proteomics group again. Michael Quintero, MS SMG Prod. Manager/Research Scientist King Industries, Inc."
"Absolutely excellent and professional service, friendly staff, will definitely use again."
"Quick response, excellent work, very satisfied."
"Qishan is extremely prompt and helpful."
"Qishan is extremely prompt and helpful."
"Machine was down but he pulled through anyways"
"Excellent service all around!"
"Excellent service and turnaround time"
RNA Epitranscriptomics & Proteomics Resource has not received any endorsements.