1352591431482_a2336ed1c2cc707a7d464a3906810725

Proteomics Core Facility

Rensselaer, New York
1352591431482_a2336ed1c2cc707a7d464a3906810725
Proteomics Core FacilityCenter for Functional GenomicsRensselaer, New York
  • 41 Services
  • 1 Member

CFG core laboratories provide services to the research community on a fee-for-service basis. These services are available to investigators at the University at Albany, other academic institutions, and commercial entities.

The CFG provides basic laboratory services to hundreds of bioscience researchers at companies, universities, and government agencies around the world, helping them create robust, reliable results quickly and affordably.

We handle projects of any size, from single sample analyses to long-term work as collaborators or subcontractors on NIH grants. Each client is assigned a Ph.D.-level scientist to coordinate their project, meeting budget and deadline needs. CFG also has a strategic alliance with Commonwealth Biotechnologies, Inc. for projects that need to meet FDA requirements for submission of data in support of new drug applications.

    2D PAGE
    Two dimensional polyacrylamide gel electrophoresis
    $150.00 USD
    per Sample

    IPG Strip

    IPG Strip

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    Affymetrix RNA microarray
    $340.00 USD
    per Sample

    Premier service (RNA isolation to data acquisition) $400 per sample
    Full service (cDNA synthesis to data acquisition) $340 per sample
    Labeled cRNA synthesis from total RNA $310 per sample
    Rehybridization only (previously labeled sample) $75 per sample
    Data analysis only $75 per hour

    Prices do not include cost of GeneChips.

    Premier service (RNA isolation to data acquisition) $400 per sample
    Full service (cDNA synthesis to data acquisition) $340 per sample
    Labeled cRNA synthesis from total RNA $310 per sample
    Rehybridization only (previously labeled sample) $75 per sample
    Data analysis only $75 per hour

    Prices do not include cost of GeneChips.

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    Agilent bioanalyzer
    $70.00 USD
    per Chip

    $70 per chip (up to 12 samples = $5.83 per sample)

    $70 per chip (up to 12 samples = $5.83 per sample)

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    Blastocyst injection
    $4,500.00 USD
    per Sample

    per construct

    per construct

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    Cell transfection
    Price on request
    Please contact Proteomics Core Facility for more information regarding this service.
    Compound structure analysis
    $100.00 USD
    per Sample
    Please contact Proteomics Core Facility for more information regarding this service.
    DNA library screen
    $3,500.00 USD
    per Sample

    Genomic - $3500
    cDNA - $3500

    Genomic - $3500
    cDNA - $3500

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    ESC cell culture
    Embryonic stem cell culture
    $150.00 USD
    per Clone
    Please contact Proteomics Core Facility for more information regarding this service.
    Embryo isolation
    $250.00 USD
    per Prep

    per procedure

    per procedure

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    Embryonic stem (ES) cell electroporation
    $7,500.00 USD
    per Sample
    Please contact Proteomics Core Facility for more information regarding this service.
    Embryonic stem (ES) cell screening
    $1,500.00 USD
    per Sample

    construct

    construct

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    FACS
    Fluorescence Activated Cell Sorting
    $75.00 USD
    per Hour

    set up - $150
    sorting - $75

    set up - $150
    sorting - $75

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    FPLC
    Fast Performance liquid chromatography
    $35.00 USD
    per Hour

    Amersham

    Amersham

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    Flow cytometry
    $65.00 USD
    per Hour
    Please contact Proteomics Core Facility for more information regarding this service.
    Gel imaging
    $40.00 USD
    per Gel
    Please contact Proteomics Core Facility for more information regarding this service.
    Gel staining
    $20.00 USD
    per Gel

    SYPRO Ruby staining

    - Use plastic tray as staining container
    - Rinse the gel 3 times with ddH2O
    - Fix the gel with 10% methanol, 7 % acetic acid, and 83% H2O for 60 min
    - Stain the gel in SYPRO Ruby solution which is at least 10 times the volume of the gel
    - Gently shake for at least 3 hour for maximal... Show more »

    SYPRO Ruby staining

    - Use plastic tray as staining container
    - Rinse the gel 3 times with ddH2O
    - Fix the gel with 10% methanol, 7 % acetic acid, and 83% H2O for 60 min
    - Stain the gel in SYPRO Ruby solution which is at least 10 times the volume of the gel
    - Gently shake for at least 3 hour for maximal sensitivity or overnight
    - Wash the gel with 10% methanol, 7 % acetic acid, and 83% H2O for 30-60 minto decrease background fluorescence
    - Wash the gel 3 times - 5 min with ddH2O
    - Acquire gel image under a blue light transilluminator (image can be seen by hand-held UV source)
    - Store the gel in 1-2% acetic acid

    Note that use SYPRO Ruby displays difficult to stain glycoproteins and lipoproteins

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    Genomic DNA extraction
    $25.00 USD
    per Sample

    Genomic DNA isolation, midi - $50 per sample
    Genomic DNA isolation (blood, clinical), mini - $55 per sample
    Genomic DNA isolation, genotyping, mini - $25 per sample*

    Our customized services range from gene isolation and characterization, cloning and expression plasmid design and construction to gene expression... Show more »

    Genomic DNA isolation, midi - $50 per sample
    Genomic DNA isolation (blood, clinical), mini - $55 per sample
    Genomic DNA isolation, genotyping, mini - $25 per sample*

    Our customized services range from gene isolation and characterization, cloning and expression plasmid design and construction to gene expression studies and protein expression and characterization.

    Major instrumentation in the laboratory includes Applied Biosystems (AB) 3700 and 310 DNA Analyzers, AB 7900HT and 5700 quantitative PCR systems.

    *All pricing is academic. Request estimate for external pricing.

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    HPLC
    High Performance Liquid Chromatography
    $35.00 USD
    per Hour

    Agilent
    $35 per hour
    $85 per day

    Agilent
    $35 per hour
    $85 per day

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    High resolution mass spectrometry (HR-MS)
    $85.00 USD
    per Sample
    Please contact Proteomics Core Facility for more information regarding this service.
    Karyotyping
    $150.00 USD
    per Clone
    Please contact Proteomics Core Facility for more information regarding this service.
    LC Mass Spec
    Liquid chromatography-mass spectrometry
    $100.00 USD
    per Sample

    molecular weight - $100
    Protein ID - $180

    molecular weight - $100
    Protein ID - $180

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    LCM
    Laser capture microdissection
    $45.00 USD
    per Sample

    LCM capture cap $45
    LCM, set-up Set-up $150
    RNA isolation $20
    DNA isolation $20
    Protein isolation$20
    Tissue embedding $25
    Sectioning $10
    DNA verification $10
    RNA verification $20

    LCM capture cap $45
    LCM, set-up Set-up $150
    RNA isolation $20
    DNA isolation $20
    Protein isolation$20
    Tissue embedding $25
    Sectioning $10
    DNA verification $10
    RNA verification $20

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    MALDI TOF Mass Spec
    Matrix-assisted laser desorption ionization time-of-flight mass spectrometry
    $50.00 USD
    per Sample
    Please contact Proteomics Core Facility for more information regarding this service.
    Mammalian cell culture
    Price on request

    The CFG offers services in cell and tissue culture. Currently available services include:
    - Maintenance and passaging of cell lines
    - Large scale amplification of cell lines
    - Transient transfection of cell lines
    - Generation of stably transfected cell lines
    - Cellular transformation assays (colony... Show more »

    The CFG offers services in cell and tissue culture. Currently available services include:
    - Maintenance and passaging of cell lines
    - Large scale amplification of cell lines
    - Transient transfection of cell lines
    - Generation of stably transfected cell lines
    - Cellular transformation assays (colony formation in soft agar)
    - Cell banking

    Cell Line Passage, T25 flask: pricing per flask
    Cell Line Passage, T75 flask: pricing per flask
    Cell Line Passage, T150 flask: pricing per flask
    Cell Line Passage, media change: pricing per flask
    Stable transfection: pricing per cell line

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    Molecular cloning
    Price on request

    construction of transgenic or knock out insertion vector

    construction of transgenic or knock out insertion vector

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    MudPIT
    Multidimensional Protein Identification Technology
    $100.00 USD
    per Sample

    per step

    per step

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    Multiple Reaction Monitoring
    $600.00 USD
    per Run

    We used a QTRAP 6500 mass spectrometer coupled with UHPLC for the analysis. The setup fee (parameter optimization) is $600 then $35 per sample injection.

    We used a QTRAP 6500 mass spectrometer coupled with UHPLC for the analysis. The setup fee (parameter optimization) is $600 then $35 per sample injection.

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    Mycoplasma testing
    $150.00 USD
    per Sample
    Please contact Proteomics Core Facility for more information regarding this service.
    Oligonucleotide annealing
    $20.00 USD
    per Sample
    Please contact Proteomics Core Facility for more information regarding this service.
    PCR
    Polymerase chain reaction
    $10.00 USD
    per Sample

    PCR w/ gel analysis Reaction $10
    PCR w/ purification PCR product $45

    PCR w/ gel analysis Reaction $10
    PCR w/ purification PCR product $45

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    Peptide mapping
    $75.00 USD
    per Hour

    de novo

    de novo

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    Pronuclear injection
    $3,000.00 USD
    per Sample

    Pronuclear injection
    per construct

    Pronuclear injection
    per construct

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    Protein digestion
    $100.00 USD
    per Sample

    Enzymatic Digest

    Enzymatic Digest

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    Protein identification
    $180.00 USD
    per Sample
    Please contact Proteomics Core Facility for more information regarding this service.
    RNA extraction
    $60.00 USD
    per Sample

    Total RNA isolation, midi - $55 per sample
    mRNA isolation, midi - $120 per sample
    Total RNA isolation (blood), mini - $60 per sample

    Our customized services range from gene isolation and characterization, cloning and expression plasmid design and construction to gene expression studies and protein expression and... Show more »

    Total RNA isolation, midi - $55 per sample
    mRNA isolation, midi - $120 per sample
    Total RNA isolation (blood), mini - $60 per sample

    Our customized services range from gene isolation and characterization, cloning and expression plasmid design and construction to gene expression studies and protein expression and characterization.

    Major instrumentation in the laboratory includes Applied Biosystems (AB) 3700 and 310 DNA Analyzers, AB 7900HT and 5700 quantitative PCR systems.

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    SDS-PAGE
    Sodium dodecyl sulfate polyacrylamide gel electrophoresis
    $60.00 USD
    per Sample
    Please contact Proteomics Core Facility for more information regarding this service.
    Sanger Sequencing
    $10.00 USD
    per Sample

    The DNA sequencing facility at CFG offers DNA sequencing services for a wide range of templates including plasmid DNA, PCR fragments, cosmid DNA, bacterial genomic DNA, BAC and Phage DNA.

    We perform sequencing reactions using Applied Biosystems BigDye terminator chemistry. Reactions are run on ABI Prism 3730XL capillary-based DNA sequencers.
    template w/ 1 primer

    The DNA sequencing facility at CFG offers DNA sequencing services for a wide range of templates including plasmid DNA, PCR fragments, cosmid DNA, bacterial genomic DNA, BAC and Phage DNA.

    We perform sequencing reactions using Applied Biosystems BigDye terminator chemistry. Reactions are run on ABI Prism 3730XL capillary-based DNA sequencers.
    template w/ 1 primer

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    Stable cell line generation
    Price on request

    Cell culture
    The CFG offers services in cell and tissue culture. Currently available services include:
    - Maintenance and passaging of cell lines
    - Large scale amplification of cell lines
    - Transient transfection of cell lines
    - Generation of stably transfected cell lines
    - Cellular... Show more »

    Cell culture
    The CFG offers services in cell and tissue culture. Currently available services include:
    - Maintenance and passaging of cell lines
    - Large scale amplification of cell lines
    - Transient transfection of cell lines
    - Generation of stably transfected cell lines
    - Cellular transformation assays (colony formation in soft agar)
    - Cell banking
    Cell Line Passage, T25 flask: pricing per flask
    Cell Line Passage, T75 flask: pricing per flask
    Cell Line Passage, T150 flask: pricing per flask
    Cell Line Passage, media change: pricing per flask
    Stable transfection: pricing per cell line

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    Transgenic mouse
    Transgenic mouse development
    $17,200.00 USD
    per Strain
    Please contact Proteomics Core Facility for more information regarding this service.
    iTRAQ
    Isobaric tags for relative and absolute quantitation
    $4,000.00 USD
    per Sample

    4 plex $4000 (> 25 fractions)
    8 plex (> 25 fractions)

    4 plex $4000 (> 25 fractions)
    8 plex (> 25 fractions)

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    qPCR
    Quantitative PCR
    $15.00 USD
    per Sample

    Quantitative PCR is a powerful, highly sensitive technique that can be used to quantitate gene expression, determine gene copy number, detect SNPs, and detect DNA from viral and bacterial microorganisms.

    The CFG utilizes Applied Biosystems 7900HT and 5700 Sequence Detection Systems to perform qPCR experiments. Our 7900HT... Show more »

    Quantitative PCR is a powerful, highly sensitive technique that can be used to quantitate gene expression, determine gene copy number, detect SNPs, and detect DNA from viral and bacterial microorganisms.

    The CFG utilizes Applied Biosystems 7900HT and 5700 Sequence Detection Systems to perform qPCR experiments. Our 7900HT system is equipped with both 96- and 384-well blocks to facilitate high throughput analysis. We support both SybrGreen and Taqman reaction chemistries. With Taqman chemistry, a labeled probe is included in the reaction to enhance the specificity and sensitivity of target sequence detection. With SyberGreen chemistry, amplified product is detected by its interaction with the SyberGreen fluorescent dye, which selectively binds to double-stranded DNA templates. SyberGreen chemistry is less expensive, but generally requires more effort to optimize reactions. Although the Taqman chemistry is more expensive, it generally provides better specificity without the need for extensive optimization. We also offer primer and probe design services.

    All pricing is academic. Request estimate for external/commercial.

    Taqman Chemistry
    Taqman qPCR optimization $950
    Taqman qPCR, per sample $20
    Taqman RT-qPCR, per sample $30
    Primer/probe design, per target $20

    SyberGreen Chemistry
    SyberGreen qPCR optimization $1,250
    SyberGreen qPCR, per sample $15
    SyberGreen RT-qPCR, per sample $25
    Primer pair design, per target $20

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Roshan Karki gave a positive review for MALDI TOF Mass Spec:

November 20, 2012
Excellent service all around!

Roshan Karki gave a positive review for Mass Spectrometry:

August 06, 2012
Excellent service and turnaround time

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