Proteomics and Metabolomics Core Facility
About Proteomics and Metabolomics Core Facility
The Proteomics and Metabolomics Core Facility offers a wide variety of services for separation, identification, quantification, and post-translational modification analysis. Results are usually delivered by email or discussed in person.
Our Services (4)
Protein Identification
LC-MS/MS protein identification includes sample preparation, denaturation, reduction/alkylation, tryptic digestion, and mass spectrometry analysis. Typically, 90 minute gradients are used for peptide separation with MS and MS/MS in ion trap (LTQ) or orbital ion trap (LTQ-Orbitrap) instruments.
Data are reported in Scaffold... Show more »
LC-MS/MS protein identification includes sample preparation, denaturation, reduction/alkylation, tryptic digestion, and mass spectrometry analysis. Typically, 90 minute gradients are used for peptide separation with MS and MS/MS in ion trap (LTQ) or orbital ion trap (LTQ-Orbitrap) instruments.
Data are reported in Scaffold files, and instructions are provided for data interpretation and selection of high confidence results. The free Scaffold viewer is required, and it can be downloaded from Proteome Software.
Metabolomics
Discovery/Untargeted/Targeted Metabolomics/Lipidomics/Fatty Acid, HILIC/Reversed Phase UPLC separation, positive/negative mass spectrometry, metabolite identification and quantitation with library searching
Discovery/Untargeted/Targeted Metabolomics/Lipidomics/Fatty Acid, HILIC/Reversed Phase UPLC separation, positive/negative mass spectrometry, metabolite identification and quantitation with library searching
Phosphoproteomics
LC-MRM
Liquid Chromatography-Multiple Reaction Monitoring Mass Spectrometry
Liquid Chromatography-Multiple Reaction Monitoring Mass Spectrometry (LC-MRM) is used to quantify components in complex mixtures. Our laboratory uses this technique for quantification of protein biomarkers. Assay development prior to sample analysis includes synthesis and characterization of standard peptides. Note that multiple proteins can be quantified in each sample.
Liquid Chromatography-Multiple Reaction Monitoring Mass Spectrometry (LC-MRM) is used to quantify components in complex mixtures. Our laboratory uses this technique for quantification of protein biomarkers. Assay development prior to sample analysis includes synthesis and characterization of standard peptides. Note that multiple proteins can be quantified in each sample.
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Proteomics Core Manager
John Koomen
Associate Professor/Scientific Director of Proteomics Core
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Positive review for Protein Identification:
February 2014
"Yes I would."
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Positive review for ESI-MS:
December 2013
"Very responsive to questions about interpretation of results"
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Positive review for Protein Identification:
August 2013
"Many thanks!"
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Positive review for Protein Identification:
August 2013
"excellent!!!"
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Positive review for Protein Identification:
May 2013
"Thank you so much!"
Proteomics and Metabolomics Core Facility has not received any endorsements.