1340747422000

Proteomics and Metabolomics Core Facility

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Tampa, Florida, US

About Proteomics and Metabolomics Core Facility

The Proteomics and Metabolomics Core Facility offers a wide variety of services for separation, identification, quantification, and post-translational modification analysis. Results are usually delivered by email or discussed in person.

Our Services (4)


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Protein Identification

Starting at $171.70 per sample

LC-MS/MS protein identification includes sample preparation, denaturation, reduction/alkylation, tryptic digestion, and mass spectrometry analysis. Typically, 90 minute gradients are used for peptide separation with MS and MS/MS in ion trap (LTQ) or orbital ion trap (LTQ-Orbitrap) instruments.

Data are reported in Scaffold... Show more »

LC-MS/MS protein identification includes sample preparation, denaturation, reduction/alkylation, tryptic digestion, and mass spectrometry analysis. Typically, 90 minute gradients are used for peptide separation with MS and MS/MS in ion trap (LTQ) or orbital ion trap (LTQ-Orbitrap) instruments.

Data are reported in Scaffold files, and instructions are provided for data interpretation and selection of high confidence results. The free Scaffold viewer is required, and it can be downloaded from Proteome Software.

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Q Exactive Orbitrap LC-MS/MS system

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Metabolomics

Price on request

Discovery/Untargeted/Targeted Metabolomics/Lipidomics/Fatty Acid, HILIC/Reversed Phase UPLC separation, positive/negative mass spectrometry, metabolite identification and quantitation with library searching

Discovery/Untargeted/Targeted Metabolomics/Lipidomics/Fatty Acid, HILIC/Reversed Phase UPLC separation, positive/negative mass spectrometry, metabolite identification and quantitation with library searching

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Phosphoproteomics

Price on request
Request a quote for more information about this service.

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LC-MRM

Liquid Chromatography-Multiple Reaction Monitoring Mass Spectrometry
Starting at $210.35 per sample

Liquid Chromatography-Multiple Reaction Monitoring Mass Spectrometry (LC-MRM) is used to quantify components in complex mixtures. Our laboratory uses this technique for quantification of protein biomarkers. Assay development prior to sample analysis includes synthesis and characterization of standard peptides. Note that multiple proteins can be quantified in each sample.

Liquid Chromatography-Multiple Reaction Monitoring Mass Spectrometry (LC-MRM) is used to quantify components in complex mixtures. Our laboratory uses this technique for quantification of protein biomarkers. Assay development prior to sample analysis includes synthesis and characterization of standard peptides. Note that multiple proteins can be quantified in each sample.

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Bin Fang

Proteomics Core Manager
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John Koomen

Associate Professor/Scientific Director of Proteomics Core

Proteomics and Metabolomics Core Facility has not received any endorsements.