Proteomics Core

The Mass Spectrometry Resource (MSR) is a state-of-the-art biologic mass spectrometry facility providing access to all the necessary instrumentation for high-throughput, high accuracy protein identification, quantification and characterisation.

Instrumentation:
In terms of equipment, MSR avails of several mass spectrometers in order to be able to cover almost all possible experimental workflows in mass spectrometry-based proteomics. These include:

• Thermo Fisher Q-Exactive
  
• Thermo Fisher Orbitrap
  
• Thermo Fisher LTQ ion trap
  
• Agilent 6520 Quadrupole-ToF
  
• Agilent 6460 Triple Quadrupole
  
• ABI 4800 Ultra MALDI/ToF-ToF with CovalX HM2 high-mass range detector
  
• Bruker HCTUltra II ETD ion trap.
  

The AB Sciex 4800 MALDI-Tof/Tof mass spectrometer with a CovalX HM2 high mass-range detector is applied to gel-based and off-line liquid chromatography (LC)-based, high throughput MALDI-MS/MS proteomic workflows or, in linear mode, to accurate determination of the mass of intact proteins or protein complexes up to 1.2 Megadaltons.

The Q-Exactive, LTQ-Orbitrap, the ion traps, the quadrupole time-of-flight and a triple quadrupole mass spectrometers cover all possible needs in liquid-chromatography-tandem mass spectrometry.

Furthermore, the MSR offers access to LC-based workflows supporting the multi-dimensional protein identification technology (MudPIT) and a nano-LC system coupled to a MALDI target loading robot to support off-line LC MALDI-MS.

Software suite
Raw data files generated by each mass spectrometer are subjected to database search with the appropriate algorithm (Mascot, TurboSequest, Spectrum Mill) and database.

The Mass Spectrometry Resource (MSR) is a state-of-the-art biologic mass spectrometry facility providing access to all the necessary instrumentation for high-throughput, high accuracy protein identification, quantification and characterisation.

Instrumentation:
In terms of equipment, MSR avails of several mass spectrometers in order to be able to cover almost all possible experimental workflows in mass spectrometry-based proteomics. These include:

• Thermo Fisher Q-Exactive
  
• Thermo Fisher Orbitrap
  
• Thermo Fisher LTQ ion trap
  
• Agilent 6520 Quadrupole-ToF
  
• Agilent 6460 Triple Quadrupole
  
• ABI 4800 Ultra MALDI/ToF-ToF with CovalX HM2 high-mass range detector
  
• Bruker HCTUltra II ETD ion trap.
  

The AB Sciex 4800 MALDI-Tof/Tof mass spectrometer with a CovalX HM2 high mass-range detector is applied to gel-based and off-line liquid chromatography (LC)-based, high throughput MALDI-MS/MS proteomic workflows or, in linear mode, to accurate determination of the mass of intact proteins or protein complexes up to 1.2 Megadaltons.

The Q-Exactive, LTQ-Orbitrap, the ion traps, the quadrupole time-of-flight and a triple quadrupole mass spectrometers cover all possible needs in liquid-chromatography-tandem mass spectrometry.

Furthermore, the MSR offers access to LC-based workflows supporting the multi-dimensional protein identification technology (MudPIT) and a nano-LC system coupled to a MALDI target loading robot to support off-line LC MALDI-MS.

Software suite
Raw data files generated by each mass spectrometer are subjected to database search with the appropriate algorithm (Mascot, TurboSequest, Spectrum Mill) and database.

The Protein Separations Laboratory (PSL) is a facility dedicated to electrophoretic gel separation of proteins prior to their identification by downstream mass spectrometric analysis, with particular expertise in the running and subsequent analysis of large format 2D-DIGE gels.

Instrumentation:
Full equipment for protein separation by 2-D gel electrophoresis (2-DE) including
- IPGphorII and IPGphor3
- Ettan Dalt 6 and 12
- BioRad Protean Plus Dodeca cell

Imagers (both visible and fluorescent):
- Typhoon 9410 variable mode imager (for 2D-DIGE)
- BioRad GS800

Image analysis software:
- Progenesis SameSpots v4
- DeCyder v6

Expertise & Services:
The Protein Separations Laboratory provides expertise and full training in two dimensional gel electrophoresis (2D-E)

• Advice on experimental design for 2D-DIGE
  
• Sample preparation for 2-DE and 2D-DIGE (including fluorescent labelling for DIGE)
  
• Full training in all aspects of running 2-DE (both mini and large formats)
  
• Training on Typhoon variable imager
  
• Training on image analysis software and interpretation of results

The Mass Spectrometry Resource (MSR) is a state-of-the-art biologic mass spectrometry facility providing access to all the necessary instrumentation for high-throughput, high accuracy protein identification, quantification and characterisation.

Instrumentation:
In terms of equipment, MSR avails of several mass spectrometers in order to be able to cover almost all possible experimental workflows in mass spectrometry-based proteomics. These include:

• Thermo Fisher Q-Exactive
  
• Thermo Fisher Orbitrap
  
• Thermo Fisher LTQ ion trap
  
• Agilent 6520 Quadrupole-ToF
  
• Agilent 6460 Triple Quadrupole
  
• ABI 4800 Ultra MALDI/ToF-ToF with CovalX HM2 high-mass range detector
  
• Bruker HCTUltra II ETD ion trap.
  

The AB Sciex 4800 MALDI-Tof/Tof mass spectrometer with a CovalX HM2 high mass-range detector is applied to gel-based and off-line liquid chromatography (LC)-based, high throughput MALDI-MS/MS proteomic workflows or, in linear mode, to accurate determination of the mass of intact proteins or protein complexes up to 1.2 Megadaltons.

The Q-Exactive, LTQ-Orbitrap, the ion traps, the quadrupole time-of-flight and a triple quadrupole mass spectrometers cover all possible needs in liquid-chromatography-tandem mass spectrometry.

Furthermore, the MSR offers access to LC-based workflows supporting the multi-dimensional protein identification technology (MudPIT) and a nano-LC system coupled to a MALDI target loading robot to support off-line LC MALDI-MS.

Software suite
Raw data files generated by each mass spectrometer are subjected to database search with the appropriate algorithm (Mascot, TurboSequest, Spectrum Mill) and database.

The Mass Spectrometry Resource (MSR) is a state-of-the-art biologic mass spectrometry facility providing access to all the necessary instrumentation for high-throughput, high accuracy protein identification, quantification and characterisation.

Instrumentation:
In terms of equipment, MSR avails of several mass spectrometers in order to be able to cover almost all possible experimental workflows in mass spectrometry-based proteomics. These include:

• Thermo Fisher Q-Exactive
  
• Thermo Fisher Orbitrap
  
• Thermo Fisher LTQ ion trap
  
• Agilent 6520 Quadrupole-ToF
  
• Agilent 6460 Triple Quadrupole
  
• ABI 4800 Ultra MALDI/ToF-ToF with CovalX HM2 high-mass range detector
  
• Bruker HCTUltra II ETD ion trap.
  

The AB Sciex 4800 MALDI-Tof/Tof mass spectrometer with a CovalX HM2 high mass-range detector is applied to gel-based and off-line liquid chromatography (LC)-based, high throughput MALDI-MS/MS proteomic workflows or, in linear mode, to accurate determination of the mass of intact proteins or protein complexes up to 1.2 Megadaltons.

The Q-Exactive, LTQ-Orbitrap, the ion traps, the quadrupole time-of-flight and a triple quadrupole mass spectrometers cover all possible needs in liquid-chromatography-tandem mass spectrometry.

Furthermore, the MSR offers access to LC-based workflows supporting the multi-dimensional protein identification technology (MudPIT) and a nano-LC system coupled to a MALDI target loading robot to support off-line LC MALDI-MS.

Software suite
Raw data files generated by each mass spectrometer are subjected to database search with the appropriate algorithm (Mascot, TurboSequest, Spectrum Mill) and database.