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Proteomics and Metabolomics Facility

13 Orders Completed
Fort Collins, Colorado, US

About Proteomics and Metabolomics Facility

The mission of the Proteomics and Metabolomics Facility is to serve as an enabling resource for research and development programs at Colorado State University.

We strive to build mass spectrometry instrument capabilities and expertise that exceed the normal resources of individual research programs, and make those technologies available as a shared resource. We also aim to provide an environment rich in expertise and educational resources, and to foster collaboration across the CSU community and beyond.

The Proteomics and Metabolomics Facility at Colorado State University has recently been recognized as a Center of Innovation by the Waters Corporation for their contributions to the field of biological mass spectrometry.

Our Services (23)


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ICP-MS

Inductively Coupled Plasma Mass Spectrometry
Price on request

Targeted analysis of 26 elements with absolute quantitation. Custom assays can also be developed upon request.


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Metabolite Profiling

Price on request

UPLC-MS: Our standard UPLC-MS acquisition workflow involves a reverse phase chromatographic separation (C8 or C18 column) which provides efficient separation of moderately polar to nonpolar metabolites. We utilize a Waters instrumentation platform which enables operation in MS^E mode enabling the simultaneous acquisition of MS data and indiscriminant fragmentation spectra (i.e. no precursor ion selection) in alternating scans. Feature detection and alignment are performed using the open source XCMS package and subsequent dataset wide correlational grouping and compound annotation is performed using in-house tools.

GC-MS: Standard workflows for non-targeted metabolite profiling by GC-MS are based on the use of a TG-5MS column which provides efficient separation of both highly polar and nonpolar metabolites.


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Protein Identification

Price on request

Protein digestion and analysis using an Orbitrap Velos mass spectrometer. Data analysis using Mascot and Scafold

Thermo Scientific Orbitrap Velos LC-Mass Spec Proteomics

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Quantitative Proteomics

Price on request

Quantitative Proteomics by spectral counting. Data acquisition on an Orbitrap Velos mass spectrometer.


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Targeted Mass Spectrometry (MRM/SRM)

Multiple Reaction Monitoring/Selected Reaction Monitoring Mass Spectrometry
Price on request

MRM analysis for both protein and small molecule quantitation.

Contact us for pricing details.


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MALDI TOF MS

Matrix-assisted laser desorption ionization time-of-flight mass spectrometry
Price on request

MS analysis by MALDI-TOF. Annotated spectrum and/or peak list is provided.


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LC-MS/MS

Liquid chromatography tandem mass spectrometry
Price on request
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Waters Xevo G2 QTOF

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Proteomics Consulting

Price on request

Experimental design and data analysis


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GC-MS

Gas Chromatography Coupled Mass Spectrometry
Price on request

Derivatization and liquid injection enables detection of a large number of small polar compounds including simple sugars, amino acids, and small organic acid.

Volatile analysis can be performed using headspace/SPME autosampler.


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Metabolite Quantification

Price on request

Targeted analysis is performed on a Waters TQ-S tandem quad mass spectrometer coupled with UPLC. We can performed custom MRM assay development for your research needs.

Currently available pre-developed assays include:

  • steroids
  • plant phytohormones
  • endocannabinoids
  • bile acids
  • Tryptophan Metabolism
  • CBD
  • Central Carbon Metabolism
  • dicyclic GMP

Pricing:
Non-CSU Academic: $90/hour
Commercial: $150/ hour


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Non-Targeted Metabolomics

Price on request

UPLC-MS: Our standard UPLC-MS acquisition workflow involves a reverse phase chromatographic separation (C8 or C18 column) which provides efficient separation of moderately polar to nonpolar metabolites. We utilize a Waters instrumentation platform which enables operation in MS^E mode enabling the simultaneous acquisition of MS data and indiscriminant fragmentation spectra (i.e. no precursor ion selection) in alternating scans. Feature detection and alignment are performed using the open source XCMS package and subsequent dataset wide correlational grouping and compound annotation is performed using in-house tools.

GC-MS: Standard workflows for non-targeted metabolite profiling by GC-MS are based on the use of a TG-5MS column which provides efficient separation of both highly polar and nonpolar metabolites.


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Spectroscopy

Price on request
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Tandem Mass Spectrometry Methods

Price on request
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Mass Spectrometry

Price on request
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MALDI Mass Spectrometry Methods

Price on request
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Protein Sequencing

Price on request
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Imaging & Spectroscopy

Price on request
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Omics

Price on request
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Other Mass Spectrometry Methods

Price on request
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Metabolomics

Price on request
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Liquid Chromatography Coupled Mass Spectrometry Methods

Price on request
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Protein Services

Price on request
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Proteomics

Price on request
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  • Positive review for MALDI TOF MS:

    March 2018

    "Excellent communication, very good commitment to finish the goals and easy to set-up"

Proteomics and Metabolomics Facility has not received any endorsements.