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Proteomics and Mass Spectrometry Facility

ic 9.0/10 Overall Rating 97% Positive
ic 152 Orders Completed
St. Louis, Missouri, US

About Proteomics and Mass Spectrometry Facility

The Danforth Center’s Proteomics & Mass Spectrometry Facility develops new tools and provides high quality sample analysis in proteomics, mass spectrometry and related analytical fields.

The Proteomics & Mass Spectrometry Facility, under the direction of Dr. Bradley Evans, has processed thousands of... Show more »

The Danforth Center’s Proteomics & Mass Spectrometry Facility develops new tools and provides high quality sample analysis in proteomics, mass spectrometry and related analytical fields.

The Proteomics & Mass Spectrometry Facility, under the direction of Dr. Bradley Evans, has processed thousands of service samples submitted from research laboratories located at the Danforth Center as well as from academic and commercial institutions worldwide. In addition, the facility has initiated research activities designed to expand analytical capabilities and has established collaborations with many principal investigators at various institutions. These activities have produced exciting results and publications, which are a testament to the facility's high standards and growing reputation.

With highly specialized capabilities and research initiatives, the facility is well-prepared and positioned to serve as a regional, national and international resource in the fast developing fields of proteomics and metabolomics.

We have an in-house collection of microorganisms too numerous to list; as a plant-science focused institution we of course also have access to plants, including, but not limited to: Arabidopsis, Setaria viridis, sorghum, soy bean, tomato, corn, Camelina, Cassava, others. We can and have also worked with human and animal primary tissue and cell-lines.

Instrumentation:
- LTQ-Orbitrap Velos Pro with 3000 RSLCnano-LC or Advion Triversa,
-6500 QTrap with Shimadzu UFLC-XR UHPLC and Eksigent 200 MicroLC
-Q-Exactive with Agilent 1200 CapLC and Eksigent NanoLC 2D Ultra
-Agilent 5975 GC-MS
-Thermo ITQ 900 GC-MS/FID
-Thermo Trace GC-FID
-Waters ACQUITY UPLC with Acutag for amino acid analysis
-Beckman System-GOLD, Agilent 1100 and Shimadzu Prominence HPLC systems for offline fractionation or UV-Vis/fluorescence analysis
-Akta Explorer FPLC

Publications:
http://www.danforthcenter.org/scientists-research/core-technologies/proteomics-mass-spectrometry/publications

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Our Services (34)


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Gas Chromatography (GC)

Gas Chromatography
Starting at $75.00 per hour
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GC-MS

Gas Chromatography Coupled Mass Spectrometry
Starting at $95.00 per hour
Request a quote for more information about this service.
GC-FID

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Isoprenoid Biosynthetic Intermediate Analysis

Starting at $195.00 per sample

Includes sample preparation, LC-MS/MS and data analysis for separation and quantification of 4 of the intermediates of the Non-mevolanate/methlerythritol/deoxyxyulose isoprenoid biosynthesis (MEP/DXP) pathway (DXP, MEP, CDP-ME, and cMEPP). The sample preparation is performed in house and samples are analyzed using a 20 minute run.

Includes sample preparation, LC-MS/MS and data analysis for separation and quantification of 4 of the intermediates of the Non-mevolanate/methlerythritol/deoxyxyulose isoprenoid biosynthesis (MEP/DXP) pathway (DXP, MEP, CDP-ME, and cMEPP). The sample preparation is performed in house and samples are analyzed using a 20 minute run.

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LC-MS

Liquid Chromatography-Coupled Mass Spectrometry
Starting at $130.00 per hour

6500 QTRAP Liquid chromatography-mass spectrometry $130/hour
Q-Exactive Liquid chromatography-mass spectrometry $200/hour
LTQ-Velos Pro Orbitrap Liquid chromatography-mass spectrometry $260/hour

For large projects requiring multiple days of data acquisition, we are offering a daily rate of $500(academic)/ $750 (commercial)... Show more »

6500 QTRAP Liquid chromatography-mass spectrometry $130/hour
Q-Exactive Liquid chromatography-mass spectrometry $200/hour
LTQ-Velos Pro Orbitrap Liquid chromatography-mass spectrometry $260/hour

For large projects requiring multiple days of data acquisition, we are offering a daily rate of $500(academic)/ $750 (commercial) for the LTQ-Velos Pro Orbitrap and $425 (academic) and $575 (commercial). Contact us for details.

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Thermo Scientific Orbitrap Velos LC-Mass Spec Q Exactive Orbitrap LC-MS/MS system AB Sciex QTRAP

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Label Free Quantitative Proteomics

Price on request

Contact us for details

Contact us for details

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Metabolomics

Price on request

Untargeted Metabolomics (10 Samples, Academic)
1 hour Sample Preparation - $85.00
5 hours Q-Exactive (30 minutes per sample) - $1000.00
8 hours Data Analysis - $800.00
TOTAL: $1885.00
Your project may be different, contact us for details

Untargeted Metabolomics (10 Samples, Academic)
1 hour Sample Preparation - $85.00
5 hours Q-Exactive (30 minutes per sample) - $1000.00
8 hours Data Analysis - $800.00
TOTAL: $1885.00
Your project may be different, contact us for details

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Phosphoproteomics

Price on request
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Protein Identification

Starting at $340.00 per sample

Protein identification from gel band
We can start from a gel band that has visible protein in it when stained by Coomassie. We can also use Silver stained gel bands, but the results may be affected by the harsher staining conditions and lower abundance. Your project may be different, contact us for details.

*Protein... Show more »

Protein identification from gel band
We can start from a gel band that has visible protein in it when stained by Coomassie. We can also use Silver stained gel bands, but the results may be affected by the harsher staining conditions and lower abundance. Your project may be different, contact us for details.

Protein identification from solution
We can start with a cell pellet, tissue or IP or other types of pull-down samples. On-bead digests work well for IP/AP samples. Contact us for more details.

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Quantitative Proteomics

Price on request

A typical experiment may consist of something like this:

Quantitative Proteomics, (TMT, 6 samples 3 biological replicates, Academic)
18 hours Sample Preparation- $1530.00
3 HPLC (makes 12 fractions of each pooled replicate)- $270.00
18 Protein Quantitation (CBX Assay) -$270.00
18 Protein Digestions- $540.00
3 TMT... Show more »

A typical experiment may consist of something like this:

Quantitative Proteomics, (TMT, 6 samples 3 biological replicates, Academic)
18 hours Sample Preparation- $1530.00
3 HPLC (makes 12 fractions of each pooled replicate)- $270.00
18 Protein Quantitation (CBX Assay) -$270.00
18 Protein Digestions- $540.00
3 TMT Labeling Kits- $1650.00
21 Sample Cleanup (Spin Column) -$525.00
5 Days Orbitrap Time at Daily Rate (2 hours per fraction plus column washing)- $2500.00
8 Hours Data analysis- $800.00
TOTAL: $8085.00

Your project may differ, contact us for details

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iTRAQ Protein Labeling

Isobaric Tags for Relative and Absolute Quantitation
Price on request

iTRAQ/TMT

A typical experiment may consist of something like this:

Quantitative Proteomics, (TMT, 6 samples 3 biological replicates, Academic)
18 hours Sample Preparation- $1530.00
3 HPLC (makes 12 fractions of each pooled replicate)- $270.00
18 Protein Quantitation (CBX Assay) -$270.00
18 Protein Digestions-... Show more »

iTRAQ/TMT

A typical experiment may consist of something like this:

Quantitative Proteomics, (TMT, 6 samples 3 biological replicates, Academic)
18 hours Sample Preparation- $1530.00
3 HPLC (makes 12 fractions of each pooled replicate)- $270.00
18 Protein Quantitation (CBX Assay) -$270.00
18 Protein Digestions- $540.00
3 TMT Labeling Kits- $1650.00
21 Sample Cleanup (Spin Column) -$525.00
5 Days Orbitrap Time at Daily Rate (2 hours per fraction plus column washing)- $2500.00
8 Hours Data analysis- $800.00
TOTAL: $8085.00

Your project may differ, contact us for details

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Plant Hormone Analysis

Starting at $213.00 per sample

Includes sample preparation, LC-MS/MS and data analysis for: Jasmonic acid (JA), Salicyclic acid (SA), Abscisic acid (ABA), Indole-3-acetic acid (IAA), jasmonoyl-isoleucine (JA-Ile), 12-oxo-phytodienoic acid (OPDA), Indole-3-acetyl-aspartic acid (IAA-Asp).

Includes sample preparation, LC-MS/MS and data analysis for: Jasmonic acid (JA), Salicyclic acid (SA), Abscisic acid (ABA), Indole-3-acetic acid (IAA), jasmonoyl-isoleucine (JA-Ile), 12-oxo-phytodienoic acid (OPDA), Indole-3-acetyl-aspartic acid (IAA-Asp).

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Ion Exchange Chromatography

Starting at $55.00 per sample

Detection and quantitation of cations and anions at sub part per million concentrations. We can report these ions: sodium, potassium, lithium, magnesium, calcium, manganese, ammonium, quantenary amines, chloride, cholite, cholrate, fluoride, bromide, phosphate, sulfate, sulfide, acetate, formate, nitrate, nitrite, others on request.

Detection and quantitation of cations and anions at sub part per million concentrations. We can report these ions: sodium, potassium, lithium, magnesium, calcium, manganese, ammonium, quantenary amines, chloride, cholite, cholrate, fluoride, bromide, phosphate, sulfate, sulfide, acetate, formate, nitrate, nitrite, others on request.

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Amino Acid Analysis

Starting at $75.00 per sample

Free Amino Acid Analysis
This service generates data that will report of the amino acids not bound by protein in the sample. For free amino acid analysis we need a minimum of 150 mg of tissue or sample.

Free AAA: $75 per sample

Turnaround time:
- standard = 2 weeks
- priority = 3 days

Deliverables:
Table of results... Show more »

Free Amino Acid Analysis
This service generates data that will report of the amino acids not bound by protein in the sample. For free amino acid analysis we need a minimum of 150 mg of tissue or sample.

Free AAA: $75 per sample

Turnaround time:
- standard = 2 weeks
- priority = 3 days

Deliverables:
Table of results with picomoles of different amino acids, and retention times. Can customize the tables based on researcher needs.

Hydrolyzed Amino Acid Analysis
This service generates data that will report on the abundance of amino acids bound by protein in the sample, also referred to as total Amino acid Analysis. For hydrolyzed amino acid analysis we need a minimum of 3 mg of tissue or sample.

Hydrolyzed AAA: $90 per sample

Turnaround time:
- standard = 2 weeks
- priority = 3 days

Deliverables:
Table of results with picomoles of different amino acids, and retention times. Can customize the tables based on researcher needs.

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Proteomics

Price on request
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Mass Spectrometry Method Development

Price on request
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Mass Spectrometry

Price on request
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LC-QQQ-MS

Liquid chromatography triple quadrupole mass spectrometry
Price on request
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Assay Development

Price on request
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Biology

Price on request
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Project Management

Price on request
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Analytical Chemistry Services

Price on request
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Protein Services

Price on request
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Imaging & Spectroscopy

Price on request
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Spectroscopy

Price on request
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Chromatography

Price on request
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Biochemistry & Molecular Biology

Price on request
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Other Mass Spectrometry Methods

Price on request
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Separation/Purification Services

Price on request
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Protein Sequencing

Price on request
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Omics

Price on request
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Liquid Chromatography (LC)

Price on request
Request a quote for more information about this service.

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Agriscience

Price on request
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Chemistry and Materials

Price on request
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Liquid Chromatography Coupled Mass Spectrometry Methods

Price on request
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aaanalysis_customerinstructions_2013.pdf

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in-gel-digestion-protocol.pdf

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plant-hormone-analysis_customer-instructions_2014.pdf

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protein-extraction-clean-up-and-assay.pdf

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ziptip-protocol.pdf

  • Positive review for Amino Acid Analysis:

    September 2018

    "Amazing service, great support and staff. It was a pleasure doing science with them!"

  • Positive review for Protein Identification:

    July 2018

    "Really professional and really helpful"

  • Positive review for Mass Spectrometry:

    June 2018

    "Absolutely will work with them again, and plan to very soon. The team under Dr. Evans is very helpful and very knowledgeable and the information I received was way beyond expectation - complete, thorough analysis at an extremely reasonable price. Cannot recommend them highly enough."

  • Positive review for Amino Acid Analysis:

    April 2018

    "Brad and Jia, are great, they were quick to reply to my questions. Scientifically, they know stuff, and the analytical science. I tried other labs in the past, with the same procedure, non were as good as Brad and Jia."

  • Positive review for Amino Acid Analysis:

    February 2018

    "Very good service !!"

  • Positive review for Protein Identification:

    October 2017

    "good and timely service"

  • Positive review for LC-MS:

    August 2017

    "Excellent service, nice team, satisfied with results. Cheaper than other quotes. Would definitely use again."

  • Positive review for Metabolomics:

    June 2017

    "Brad is very helpful. I had a great experience collaborating with Brad and his facility."

  • Positive review for Amino Acid Analysis:

    April 2017

    "Really good service, quickly and good results"

  • Positive review for Amino Acid Analysis:

    March 2017

    "Yes."

  • Positive review for GC-MS:

    December 2016

    "Very good service, timely done, knowledgeable, patient. Will do it again!"

  • Positive review for LC-MS:

    November 2016

    "their quality of work met my expectations, they usually respond in a reasonable time and they know how to communicate with people. if i have to rank them i will give them 4 out of 5 stars."

  • Positive review for Ion Exchange Chromatography:

    May 2016

    "work was done in a timely and professional manner....I will use Brad's service in the future."

  • Positive review for Ion Exchange Chromatography:

    April 2016

    "Brad is knowledgeable, courteous and he delivered results on time....can't ask for anything more!"

  • Positive review for GC-MS:

    November 2015

    "Very quick to respond and very helpful when I had questions."

  • Positive review for Amino Acid Analysis:

    October 2015

    "Extremely happy with my experiences with Proteomics and Mass Spec in St. Louis. Dr. Evans answered my questions for months as I worked to plan my project (Biology Master's Thesis work). When I was finally ready to send my samples off he provided very helpful advice. No other lab was willing to work with me because of the relatively small number of samples I planned to analyze (65) and the unusual nature of my samples (fecal sac samples from passerine nestlings). This was my first experience having samples sent away for analysis and Proteomics and Mass Spec and the Science Exchange helped me figure things out during every stage of the process. Would absolutely recommend this lab."

  • Positive review for PAGE:

    July 2015

    "Great job. Great communication about all aspects of the work. Very helpful. Thanks again!"

  • Positive review for GC-MS:

    April 2015

    "Great contact person and great team they have there! They were very excellent in not just doing the work but also communicating on what your needs are and working with you! I am going to be using these guys on a regular basis! :)"

  • Positive review for Ion Exchange Chromatography:

    August 2014

    "This project was very tricky and we have no experience with the methods used. Brad was great! He patiently explained the various options and helped us finalize the research plan. The work was completed ahead of schedule. The output report was easy to understand. Brad answered some follow-up questions as well. We will work with this vendor again!"

Proteomics and Mass Spectrometry Facility has not received any endorsements.