FACILITY OPENS 15 June 2020.
The Proteomics and Mass Spectrometry Facility is equipped with an ThermoScientific Orbitrap Elite mass spectrometer for high resolution and high mass accuracy analysis. It is coupled with a nano HPLC, increasing its capacity to analyze more complex protein mixtures. The facility also has a Bruker Autoflex MALDI for quick analysis of tryptic digests of pure proteins. The facility offers in-gel digestion and subsequence analysis for protein identification. The facility also has an in-house version of Mascot that provides customers with the option of loading a database to search for protein identification. **Recent Publications** - Shen L, Macnaughtan MA, Frohlich KM, Cong Y, Goodwin OY, Chou CW, LeCour L Jr, Krup K, Luo M, Worthylake DK. Multipart Chaperone-Effector Recognition in the Type III Secretion System of Chlamydia trachomatis. *J Biol Chem*. 2015 Nov 20;290(47):28141-55. - Mills-Lujan K, Andrews DL, Chou CW, Deom CM. The roles of phosphorylation and SHAGGY-like protein kinases in geminivirus C4 protein induced hyperplasia. *PLoS One*. 2015 Mar 27;10(3):e0122356. - Flores FP, Singh RK, Kerr WL, Phillips DR, Kong F. In vitro release properties of encapsulated blueberry (Vaccinium ashei) extracts. *Food Chem.* 2015 Feb 1;168:225-32. - Frohlich KM, Hua Z, Quayle AJ, Wang J, Lewis ME, Chou CW, Luo M, Buckner LR, Shen L. Membrane vesicle production by Chlamydia trachomatis as an adaptive response. *Front Cell Infect Microbiol.* 2014 Jun 10;4:73. - Kim YK, Phillips DR, Tao Y. Evidence for no sexual isolation between Drosophila albomicans and D. nasuta. *Ecol Evol.* 2013 Jul;3(7):2061-74. - Kim YK, Ruiz-García M, Alvarez D, Phillips DR, Anderson WW. Sexual isolation between North American and Bogota strains of Drosophila pseudoobscura. *Behav Genet.* 2012 May;42(3):472-82.
Samples need to be ready for analysis as facility does not include any sample clean up. Analysis is performed on a Bruker Esquire 3000 Plus ion trap. MS/MS can be requested at time of analysis of a specified ion at no extra cost. A mass spectrum can be sent via e-mail as a emf (picture) file and/or x,y coordinate file for plotting with software such as Excel. Turn around time is general less than 3 business days.
High Resolution Accurate Mass ESI by sample infusion. Only manipulation of sample would be for dilution or solvation. Analysis will be performed on one of the following:Bruker Impact II QTOF ($50), Thermo Scientific Velos Orbitrap mass spectrometer ($55), Bruker SolariX 12T FTICR MS ($60).
Protein ID by Peptide Mass Fingerprinting (in-gel digestion, MALDI-MS, & database search). This is by TOF-MS and is good for ID of single protein in clean gel band. Performed on a Bruker Autoflex.
Protein ID of gel band by LC-MS/MS (60 min. run) (includes: in-gel trypsin digestion, LC-MS/MS, & one database search) Good for searching band(s) that are suspected to contain numbers proteins. Thermo Scientific Velos Orbitrap mass spectrometer coupled to a nano-LC system.
Sample is analyzed by a Bruker Autoflex MALDI-TOF. Samples are routinely mixed with DHB matrix for analysis but facility also has alpha-cyano-4-hydroxycinnamic acid and sinapinic acid matrices available. Data is provided as an emf (picture) file and/or x,y coordinates that can be plotted using Excel.
"People here work very professionally and communicate with me very well regarding experiment details and data analysis. Also, the turn-over time is much shorter then what we expected."
"Great job as always! Fast and thorough"
"The many samples were finished way earlier than expected, especially for it being holiday time. The data was returned in a way that exceeding expectations. Thank you for the great work guys!"
"The Proteomics and Mass Spectrometry Core Facility provided HRMS services quickly and generated high quality data. The scientific staff are very responsive and did a very nice job."
"Excellent communication. Very clear timelines and they delivered within that timeline. HRMS results were summarized in an Excel spreadsheet as well as in a PowerPoint with spectra and annotated with notes. I've worked with several other HRMS facilities and these were among the most thorough reports that I've received. I should also note that they successfully obtained data on two compounds that we could not observe ions with our in house low-res LCMS."
"Great service. I would like to work with 'Proteomics and Mass Spectrometry Core Facility' again."
"We always use this laboratory, and are always pleased with the fast and great service!"
"Great service! Fully professional, knowledgeable and helpful!"
"Always a great service, good data, fast turnaround. Have used successfully for many years."
"Not only the product was excellent, but the willingness to engage in a dialog to make sure that the experiment was the correct one was outstanding. I will certainly work with them again in the future!"
"Results were in sooner than expected."
"The Proteomics and Mass Spectrometry Core Facility provided high quality service with fast turnaround. Couldn't be happier."
"Dennis Phillips was very responsive to all of my requests for information. The spectra were provided in a timely fashion with complete details on the analysis as requested. This was a great experience. I highly recommend this mass spectroscopy group."
Proteomics and Mass Spectrometry Core Facility has not received any endorsements.