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Protein Structure Core Facility

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6 Orders Completed
Omaha, Nebraska, US

About Protein Structure Core Facility

The Protein Structure Core Facility operates in the department of Biochemistry and Molecular Biology, on the UNMC campus. The facility performs protein sequencing, amino acid analysis and peptide mapping on a fee-for-service basis. It is located on the first floor of the Durham Research Center

Our Services (7)


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Hydrolyzed Amino Acid Analysis

Starting at $140.00 per sample

We provide high quality, accurate amino acid analysis, with seven standards and hydrolysis of NIST BSA included with every run. Seventeen (17) amino acids are determined. Other amino acids can be quantitated upon request. Samples can be either liquid or blotted onto PVDF membrane. We perform vapor hydrolysis using 6N HCl with... Show more »

We provide high quality, accurate amino acid analysis, with seven standards and hydrolysis of NIST BSA included with every run. Seventeen (17) amino acids are determined. Other amino acids can be quantitated upon request. Samples can be either liquid or blotted onto PVDF membrane. We perform vapor hydrolysis using 6N HCl with phenol and sodium sulfite added. 20-24 hours of hydrolysis under argon is generally used. Amino acids are then analyzed with a Hitachi L8800. The Hitachi L8800 uses an ion exchange column to first separate the amino acids. Color is developed (post-column-derivatization) with ninhydrin. This method may take samples which are contaminated with salts, urea, and small amounts of detergents. A sample of between 0.5 micrograms to 10 micrograms is necessary for analysis. Replicates are charged $40 each. Turn around time may be as long as two weeks.

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Hitachi L8600 Ninhydrin

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Edman Degradation

Starting at $500.00 per sample

The PSCF performs Edman degradation using a Shimadzu PPSQ33A, determining the amino acid sequence of proteins or peptides. This instrument delivers precise volumes of reagents to a cartridge where the polypeptide is immobilized on either an acid-etched glass filter coated with polybrene or a PVDF membrane. At each cycle the... Show more »

The PSCF performs Edman degradation using a Shimadzu PPSQ33A, determining the amino acid sequence of proteins or peptides. This instrument delivers precise volumes of reagents to a cartridge where the polypeptide is immobilized on either an acid-etched glass filter coated with polybrene or a PVDF membrane. At each cycle the derivatized amino acid is transferred to the HPLC for analysis and quantification. It is possible to obtain sequence data on as little as 5 picomoles of clean peptide or 10picomoles of clean protein. The amount of protein or peptide necessary to obtain unambiguous sequence is dependent on the sample. Turnaround time is generally within one week. Price listed is for minimum number of cycles (amino acids identified from the N-terminus (5). Additional amino acids may be added at a charge of $50 per cycle. Deliverables include an electronic report including consensus calls from two experienced scientists, chromatograms from each cycle, and a description of the process.

Set Up charge $250

Per Cycle charge $50

Minimum charge $500

Radioactive Samples: Additional per cycle $10

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N-terminal Deblocking

Starting at $120.00 per sample
Request a quote for more information about this service.

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Protein Sequencing

Price on request
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Biochemistry & Molecular Biology

Price on request
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Protein Services

Price on request
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Biology

Price on request
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Laurey Steinke

Assistant Professor, Department of Biochemistry and Molecular Biology

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  • Positive review for Edman Degradation:

    June 2017

    "Very professional, prompt, and thorough service for N-terminal sequencing."

  • Positive review for Hydrolyzed Amino Acid Analysis:

    April 2014

    "Work done in a timely fashion with good results."

  • Positive review for Hydrolyzed Amino Acid Analysis:

    October 2013

    "Knowledgeable and was able to answer any questions. Fast and repeatable results delivered."

  • Positive review for Hydrolyzed Amino Acid Analysis:

    January 2013

    "Laurey and her lab did a great job on my amino acid analyses. She was a great communicator and provided excellent support."

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    Marcus WelkerSeptember 2013

    During Winter 2013 I used Science Exchange for the first time to find a lab to analyze water samples for dissolved free amino acids.

    Laurey and her team did an excellent job working with me upfront to outline and work together on the protocol. They then rapidly analyzed my samples and provided valuable results.

    I would highly recommend UNMC for amino acid analyses.

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