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Protein RST, LLC

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Hagerstown, Maryland, US

About Protein RST, LLC

Protein RST, LLC produces high quality biologically active recombinant proteins and develops protein-related technologies for scientific research and industrial applications, as well as provides scientists with protein research services of DNA cloning, protein expression, protein purification, protein characterizations, and enzyme assays.

Protein RST has established platforms for expression of toxic and aggregation-prone proteins.

Our Services (13)


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Recombinant Protein Expression

Price on request

Recombinant Difficult Protein Expression

Expressing recombinant proteins in E. coli is sometimes challenging with problems of low expression yield, slow cell culture growth, unwanted mutations, and formation of inclusion bodies. At Protein RST, we have been developing technological platforms for producing such difficult proteins in functional forms and at milligrams, and have achieved significant successes. Protein RST, LLC would like to work with our customers to produce challengeable proteins.

B. subtilis

difficult proteins protein quality control Heat shock proteins chaperones B. subtilis E. coli Show 6 more tags Show less

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Bacterial Protein Expression

Price on request

Protein expression with E. coli: E. coli expression systems are well established for many proteins that do not need glycosylations for folding, solubility, and activities.

Protein expression with B. subtilis: B. subtilis secretes proteins into medium and serves as a platform for production of certain types of proteins.

fermentation optimization B. subtilis E. coli

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Cell-Free Protein Expression

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Protein Expression in Mammalian Cells

Mammalian cell based recombinant protein production
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Protein Purification

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  • Chromatographic separations based on mechanisms of anion exchange, cation exchange, hydroxylapatite, and gel filtration.
  • Purification of His-tagged proteins with one step affinity binding chromatography allowing for better recovery of small scale proteins. Conventional chromatographic purification can be conducted when it is necessary to remove minor contaminated proteins from the His-tagged protein purified with affinity chromatography.
  • Remove aggregated and denatured proteins after long storage.

gel filtration chromatography ion exchange chromatography Affinity chromatography hydroxylapatite chromatogrphy batch purification ammonium sulfate fractionation dialysis Show 7 more tags Show less

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Antibody Purification

Price on request

We have extensive experience in antibody purification at large scales.


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Protein Characterization

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Native PAGE SDS-PAGE Western blotting protein quantification kinetic assays Spectrophotometric assays centrifugation enzyme assays Show 8 more tags Show less

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Molecular Cloning

Price on request
  • We provide molecular biology services of gene cloning, plasmid construction, PCR, and mutagenesis.
  • We provide customized preparation of E. coli electrocompetent cells.

mutagenesis PCR Cloning plasmid construction DNA quantification electroporation competent cell preparations Show 7 more tags Show less

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Fusion Proteins

Price on request
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toxicity solubilization affinity binding signal sequences translocation

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Protein Crystallization Screening and Optimization

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Phage Display Library Construction and Screening

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Bioconjugation/Chemical Crosslinking

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Project Management

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Nicholas Guo

Owner

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