Protein Production Group

University of Kansas

Lawrence, Kansas, US

The COBRE Protein Production Group (PPG) focuses on the cloning, expression and purification of prokaryotic and eukaryotic proteins for COBRE and other investigators in Kansas and the region. The laboratory maintains a variety of equipment to support the production of properly folded proteins in quantities suitable for structural studies (X-ray and NMR), functional studies (catalytic or biological), label-free binding studies (SPR) and/or high throughput (HTP) screening studies.

Bacterial Protein Expression

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FPLC

Fast Performance liquid chromatography

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Baculovirus/Insect Cell Protein Expression

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Label-Free Mass Spectrometry

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Mass Spectrometry

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Experimental Design

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Advice on experiment design and protocol development

Molecular Cloning

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Molecular biology in support of protein expression (cloning, mutagenesis, tagging, chimeragenesis)

Protein Formulation

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Protein Identification

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Recombinant Protein Expression

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Polyacrylamide Gel Electrophoresis

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Site-Directed Mutagenesis

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Tandem Mass Tags

Tandem Mass Tags

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Project and Process Management

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Electrophoresis

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Tandem Mass Spectrometry Methods

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Spectroscopy

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Biology

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Biochemistry & Molecular Biology

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Chromatography

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Protein Sequencing

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Purification Services

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Nucleic Acid Services

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DNA Services

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Protein Services

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Liquid Chromatography

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Mutagenesis

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Chemistry and Materials

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Gel Electrophoresis

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Protein Production

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Imaging & Spectroscopy

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Analytical Chemistry Services

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Specialty Mass Spectrometry Methods

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