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Protein Production Group

University of Kansas
Lawrence, Kansas, US

The COBRE Protein Production Group (PPG) focuses on the cloning, expression and purification of prokaryotic and eukaryotic proteins for COBRE and other investigators in Kansas and the region. The laboratory maintains a variety of equipment to support the production of properly folded proteins in quantities suitable for structural studies (X-ray and NMR), functional studies (catalytic or biological), label-free binding studies (SPR) and/or high throughput (HTP) screening studies.

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Bacterial Protein Expression
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FPLC
Fast Performance liquid chromatography
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Baculovirus Protein Expression
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Label-Free Mass Spectrometry
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Mass Spectrometry
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Experimental Design
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Advice on experiment design and protocol development

Advice on experiment design and protocol development

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Molecular Cloning
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Molecular biology in support of protein expression (cloning, mutagenesis, tagging, chimeragenesis)

Molecular biology in support of protein expression (cloning, mutagenesis, tagging, chimeragenesis)

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Protein Formulation
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Protein Identification
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Recombinant Protein Expression
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Polyacrylamide Gel Electrophoresis
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Site-Directed Mutagenesis
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Tandem Mass Tags
Tandem Mass Tags
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Project and Process Management
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Electrophoresis
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Tandem Mass Spectrometry Methods
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Spectroscopy
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Biology
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Biochemistry & Molecular Biology
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Chromatography
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Protein Sequencing
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Purification Services
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Nucleic Acid Services
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DNA Services
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Protein Services
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Liquid Chromatography
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Mutagenesis
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Chemistry and Materials
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Gel Electrophoresis
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Protein Expression
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Imaging & Spectroscopy
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Analytical Chemistry Services
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Specialty Mass Spectrometry Methods
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