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Protein Production and Analysis Core

Orlando, Florida, US

Sanford Burnham Prebys Medical Discovery Institute's advanced Protein Production Core offers state-of-the-art resources with biochemical engineering and bioprocessing capabilities.

Specialized protein production scale-up services (65L bacterial or 25-50L insect/mammalian cell production) include optimization of production runs. Analytical services include measurement of protein-protein and protein-small molecule interactions using a variety of approaches such as surface plasmon resonance and isothermal titration calorimetry.

Assistance in experimental design and technical support are also provided by the Core’s knowledgeable staff.

Available cell lines: serum free suspension HEK293, Custom suspension CHO cells, Sf9 cells, e.Coli

Instrumentation:
The instrumentation is distributed between several labs in our facility mainly to divide mammalian and microbial production suites and a purification and... Show more »

Sanford Burnham Prebys Medical Discovery Institute's advanced Protein Production Core offers state-of-the-art resources with biochemical engineering and bioprocessing capabilities.

Specialized protein production scale-up services (65L bacterial or 25-50L insect/mammalian cell production) include optimization of production runs. Analytical services include measurement of protein-protein and protein-small molecule interactions using a variety of approaches such as surface plasmon resonance and isothermal titration calorimetry.

Assistance in experimental design and technical support are also provided by the Core’s knowledgeable staff.

Available cell lines: serum free suspension HEK293, Custom suspension CHO cells, Sf9 cells, e.Coli

Instrumentation:
The instrumentation is distributed between several labs in our facility mainly to divide mammalian and microbial production suites and a purification and analysis labs. It consists of:
- 60 L pilot fermentation reactor
- 7.5L stirred tank bioreactor
- 25 L Wave bioreactor (capacity is from 2-25L)
- AKTA FPLC system
- Shaker incubators
- CO2 shaker incubators
- Standard incubators
- MoFlo Astrios Cell Sorter
- AutoITC 200 calorimeter
- SPR system: BioRAD XPR36

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Protein Production and Analysis Core has not listed any services.

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Protein Complex Expression
Price on request

A unique service, offering, multiprotein co-expression to produce protein complexes from insect cells

A unique service, offering, multiprotein co-expression to produce protein complexes from insect cells

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Molecular Cloning
Starting at $250.00 per clone

We can provide custom vector design as part of the cloning service which includes vectors for particular expression platform services we carry. The cloning process can sometimes be difficult and time-consuming, especially if you need to create custom vectors to express proteins using multiple systems. Customers can provide cDNA of... Show more »

We can provide custom vector design as part of the cloning service which includes vectors for particular expression platform services we carry. The cloning process can sometimes be difficult and time-consuming, especially if you need to create custom vectors to express proteins using multiple systems. Customers can provide cDNA of their expression target or we can order and verify a sequence of interest. In a typical project the gene of interest can be amplified from a customer-supplies vector by PCR and clones into a specific required expression vector. Afterwards, the expression construct will be sequenced for quality control.

A typical cloning project can take 2-3 weeks.

Price starts at $250.00 depending cloning steps and status of requesting organization as a non-profit or commercial entity.

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Bacterial Protein Expression
Price on request

E.coli expression trials, per construct up to several hundred liters of recombinant protein expression, including various purification options downstream of the fermentor.

E.coli expression trials, per construct up to several hundred liters of recombinant protein expression, including various purification options downstream of the fermentor.

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Baculovirus Protein Expression
Price on request

Baculovirus Expression

Baculovirus Expression

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Protein Expression in Mammalian Cells
Mammalian cell based recombinant protein production
Price on request
Request a quote for more information about this service.
FPLC
Fast Performance liquid chromatography
Price on request

Instrumentation: AKTA FPLC system

Instrumentation: AKTA FPLC system

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Isothermal Titration Calorimetry (ITC)
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Protein Purification
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Recombinant Protein Expression
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Chromatography
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Calorimetry
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Protein Services
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Biology
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Protein Expression
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Purification Services
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Liquid Chromatography
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Physical Analysis Methods
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Protein Purification and Quantification
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Chemistry and Materials
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Engineering
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DNA
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Analytical Chemistry Services
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Biochemistry & Molecular Biology
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Nucleic Acid Services
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Protein Production and Analysis Core has not received any ratings.

Missing Avatar
2012-11-19 07:36:17 -0800

I have several projects with this core. The core staff are very responsible, easy to communicate with and always deliver the high quality products on time. I definitely will recommend this core to others.

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