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Protein Production & Analysis Core

Orlando, Florida

Sanford-Burnham’s Protein Production Core offers state-of-the-art resources with advanced biochemical engineering and bioprocessing capabilities.

Specialized protein production scale-up services (65L bacterial or 25-50L insect/mammalian cell production) include optimization of production runs. Analytical services include measurement of protein-protein and protein-small molecule interactions using a variety of approaches such as surface plasmon resonance and isothermal titration calorimetry.

Assistance in experimental design and technical support are also provided by the Core’s knowledgeable staff.

Available cell lines: HEK293, Custom suspension CHO cells, Sf9 cells, e.coli BL21 and DH3

Instrumentation:
The instrumentation is distributed between several labs in our facility mainly to divide mammalian and microbial production suites and a purification and analysis labs. It consists... Show more »

Sanford-Burnham’s Protein Production Core offers state-of-the-art resources with advanced biochemical engineering and bioprocessing capabilities.

Specialized protein production scale-up services (65L bacterial or 25-50L insect/mammalian cell production) include optimization of production runs. Analytical services include measurement of protein-protein and protein-small molecule interactions using a variety of approaches such as surface plasmon resonance and isothermal titration calorimetry.

Assistance in experimental design and technical support are also provided by the Core’s knowledgeable staff.

Available cell lines: HEK293, Custom suspension CHO cells, Sf9 cells, e.coli BL21 and DH3

Instrumentation:
The instrumentation is distributed between several labs in our facility mainly to divide mammalian and microbial production suites and a purification and analysis labs. It consists of:
- 60 L pilot fermentation reactor
- 25 L Wave bioreactor (capacity is from 2-25L)
- AKTA FPLC system
- Shaker incubators
- CO2 shaker incubators
- Standard incubators
- MoFlo Astrios Cell Sorter
- AutoITC 200 calorimeter
- SPR system: BioRAD XPR36

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    Bacterial based recombinant protein production
    Price on request
    Please contact Protein Production & Analysis Core for more information regarding this service.
    FPLC
    Fast Performance liquid chromatography
    Price on request

    Instrumentation: AKTA FPLC system

    Instrumentation: AKTA FPLC system

    « Show less
    ITC
    Isothermal titration calorimetry
    Price on request
    Please contact Protein Production & Analysis Core for more information regarding this service.
    Insect cell based recombinant protein production
    Price on request
    Please contact Protein Production & Analysis Core for more information regarding this service.
    Mammalian protein production
    Mammalian cell based recombinant protein production
    Price on request
    Please contact Protein Production & Analysis Core for more information regarding this service.
    Molecular cloning
    Price on request

    We can provide custom vector design as part of the cloning service which includes vectors for particular expression platform services we carry. The cloning process can sometimes be difficult and time-consuming, especially if you need to create custom vectors to express proteins using multiple systems. Customers can provide cDNA of... Show more »

    We can provide custom vector design as part of the cloning service which includes vectors for particular expression platform services we carry. The cloning process can sometimes be difficult and time-consuming, especially if you need to create custom vectors to express proteins using multiple systems. Customers can provide cDNA of their expression target or we can order and verify a sequence of interest. In a typical project the gene of interest can be amplified from a customer-supplies vector by PCR and clones into a specific required expression vector. Afterwards, the expression construct will be sequenced for quality control.

    A typical cloning project can take 2-3 weeks.

    Equipment:
    Wave Bioreactors

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    Protein purification
    Price on request
    Please contact Protein Production & Analysis Core for more information regarding this service.
    Recombinant protein production
    Price on request
    Please contact Protein Production & Analysis Core for more information regarding this service.
    SPR
    Surface plasmon resonance
    Price on request

    Instrumentation: BioRAD XPR36

    Instrumentation: BioRAD XPR36

    « Show less
    Visualizing Protein expression
    Price on request
    Please contact Protein Production & Analysis Core for more information regarding this service.

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Jason Li endorsed this lab:

November 19, 2012

I have several projects with this core. The core staff are very responsible, easy to communicate with and always deliver the high quality products on time. I definitely will recommend this core to others.

Endorse this lab