1333323285000

Protein and Nucleic Acid (PAN) Facility

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Stanford, California, US

About Protein and Nucleic Acid (PAN) Facility

The Protein and Nucleic Acid (PAN) Biotechnology Facility is a multifaceted biotechnology support facility associated with Stanford University Medical Center.

The PAN Biotechnology Facility offers a diverse array of instrumentation and technical capabilities and plays a significant consultative role in the... Show more »

The Protein and Nucleic Acid (PAN) Biotechnology Facility is a multifaceted biotechnology support facility associated with Stanford University Medical Center.

The PAN Biotechnology Facility offers a diverse array of instrumentation and technical capabilities and plays a significant consultative role in the application of these technologies to basic science projects. Such services include:

-Gene Expression
-Oligo Synthesis
-DNA Sequencing
-Real-Time PCR
-Pyrosequencing
-Surface Plasmon Resonance (Biacore)
-Peptide Synthesis
-Protein Analytics (Protein Sequencing/Mass Mapping)
-Mass Spectrometry
-Antibodies

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Our Services (50)


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Affymetrix Microarray

Starting at $472.00 per sample

Details: Chips are not included in the price

Human SNP Array 5.0 $472 per sample
Human SNP Array 6.0 $510 per sample

Details: Chips are not included in the price

Human SNP Array 5.0 $472 per sample
Human SNP Array 6.0 $510 per sample

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Affymetrix RNA Microarray

Starting at $507.00 per sample

Details: Chips are not included in the price

Standard Sample: Target Labeling >50ng using Affymetrix 3' IVT Express Protocol
Full Services: (cDNA synthesis to data acquisition; includes Bioanalyzer QC) $507 per sample
Labeled cRNA synthesis from total RNA $403 per sample
Hybridization and Scanning $102 per sample

Small... Show more »

Details: Chips are not included in the price

Standard Sample: Target Labeling >50ng using Affymetrix 3' IVT Express Protocol
Full Services: (cDNA synthesis to data acquisition; includes Bioanalyzer QC) $507 per sample
Labeled cRNA synthesis from total RNA $403 per sample
Hybridization and Scanning $102 per sample

Small Sample: Target Labeling 500pg to 50ng using NuGen protocol
Full Services: (cDNA synthesis to data acquisition; includes Bioanalyzer QC) $519 per sample
Labeled cRNA synthesis from total RNA $456 per sample
Hybridization and Scanning $102 per sample

Gene Arrays: $498 per sample
Full Service: Whole Transcript (WT) Sense Target Labeling to data acquisition

Exon Array: $575 per sample
Full Service: Survey of alternative splicing and gene expression - exon level analysis

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Affymetrix microRNA (miRNA) Microarray

Starting at $212.00 per sample

Details: Chips are not included in the price

Full Service: miRNA coverage of 71 organisms, including human, mouse, rat, canine, and rhesus macaque

Details: Chips are not included in the price

Full Service: miRNA coverage of 71 organisms, including human, mouse, rat, canine, and rhesus macaque

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DNA/RNA Analysis and Quality Control

Starting at $16.00 per sample

Agilent Bioanalyzer QC (per sample)
up to 12 samples for nano, 11 for pico $16
each additional sample $14

Agilent Bioanalyzer QC (per sample)
up to 12 samples for nano, 11 for pico $16
each additional sample $14

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Agilent Bioanalyzer

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Bioinformatics

Price on request

Analysis of microarray expression data

Analysis of microarray expression data

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DNA Sequencing

Starting at $750.00 per sample

Full service (Reaction not completed)
Client provides template and primer already added [PAN will provide standard primers] $8

Intermediate service
Client prepares reaction and PAN cleans up reaction $5

Economy service (Reaction completed)
Client prepares and cleans up reaction $4

96 well-plate Full service (Reaction... Show more »

Full service (Reaction not completed)
Client provides template and primer already added [PAN will provide standard primers] $8

Intermediate service
Client prepares reaction and PAN cleans up reaction $5

Economy service (Reaction completed)
Client prepares and cleans up reaction $4

96 well-plate Full service (Reaction not completed)
Template and primer already added [PAN will provide standard primers] $750 per plate

Capillary run only
Client drops off plate ready to run $300 per plate

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DNA Fragment Analysis

Starting at $6.00 per sample

Fragment Analysis (GeneScan)
Clean-up and Run $8
Run only $6

Fragment Analysis (GeneScan)
Clean-up and Run $8
Run only $6

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Edman Degradation

Starting at $228.00 per sample

Set-up fee/sample (includes first 10 Residues) $228

Per residue (>10 residues) $29

Set-up fee/sample (includes first 10 Residues) $228

Per residue (>10 residues) $29

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In situ Hybridization (ISH)

In Situ Hybridization
Starting at $102.00 per sample

Hybridization and Scanning

Hybridization and Scanning

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MALDI TOF MS

Matrix-assisted laser desorption ionization time-of-flight mass spectrometry
Starting at $33.00 per sample

Mass spectrometry by MALDI - $33
Prespotted plate $12
Zip Tip Cleanup $11

Mass spectrometry by MALDI - $33
Prespotted plate $12
Zip Tip Cleanup $11

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Oligonucleotide Synthesis

Starting at $0.40 per base

DNA per base:
› 40 nmole (standard scale): $0.4
› 200 nmole: $1.05
› 1 µmole: $2.15

Bulk Orders (per base):
› 20nmole scale (>24 oligos/order) $0.10
› 96-well plate format by request (>48 oligos/order) $0.10

RNA (per base):
› 40 nmol $6.25
› 200 nmole $8

Modifications **For any orders with 3 or more... Show more »

DNA per base:
› 40 nmole (standard scale): $0.4
› 200 nmole: $1.05
› 1 µmole: $2.15

Bulk Orders (per base):
› 20nmole scale (>24 oligos/order) $0.10
› 96-well plate format by request (>48 oligos/order) $0.10

RNA (per base):
› 40 nmol $6.25
› 200 nmole $8

Modifications **For any orders with 3 or more modifications/order, please inquire:
› Biotin $55
› Inosine $10
› Deoxyuridine $10
› 5' Phosphorylation $10
› 5' Thiolation Inquire
› Internal mixed bases No charge
› Phosphothiolation (antisense) No charge
› Other modifications Inquire

Purification:
› 40 and 200 nmole scale $8.50/oligo
› 1µmole scale $17/oligo

LNA (per base)
› All fees - inquire

**Shipping and Handling
› USA $25
› International $45

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Peptide Synthesis

Starting at $262.00 per sample

(Non-purified peptide synthesis includes analytical HPLC and Mass spec QC)

Standard scale (0.2 mmole-200 mg-500 mg)/amino acid (non purified)
Set-up fee (includes 5 AA) $219
Per Amino acid > 5 $43

Immunogen scale (25 µmole-10 mg-30 mg)/amino acid (non-purified)
Set-up fee (includes 5 AA) $143
Per Amino acid > 5... Show more »

(Non-purified peptide synthesis includes analytical HPLC and Mass spec QC)

Standard scale (0.2 mmole-200 mg-500 mg)/amino acid (non purified)
Set-up fee (includes 5 AA) $219
Per Amino acid > 5 $43

Immunogen scale (25 µmole-10 mg-30 mg)/amino acid (non-purified)
Set-up fee (includes 5 AA) $143
Per Amino acid > 5 $30

Modification - inquire

HPLC Purification $224

**Shipping and Handling
USA $25
International $45

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PhyloChip Array

Starting at $219.00 per sample

Details: Chips are not included in the price

Full Service: Comprehensive, sensitive, and quantitative analysis of microbial communities using DNA from any sample type
Array cost includes data analysis

Details: Chips are not included in the price

Full Service: Comprehensive, sensitive, and quantitative analysis of microbial communities using DNA from any sample type
Array cost includes data analysis

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Protein Identification

Starting at $175.00 per sample

Protein ID includes: In-gel digestion with trypsin; MS/MS peptide fragmentation; Mascot search against the NCBInr protein database; Report summary in pdf format.

Price per sample (for first 10 samples in an order) $175

Additional sample(s) (above 10 samples in the order) $117

Specialized database construction - inquire

Protein ID/Characterization from complex protein mixtures - N/A

Protein ID includes: In-gel digestion with trypsin; MS/MS peptide fragmentation; Mascot search against the NCBInr protein database; Report summary in pdf format.

Price per sample (for first 10 samples in an order) $175

Additional sample(s) (above 10 samples in the order) $117

Specialized database construction - inquire

Protein ID/Characterization from complex protein mixtures - N/A

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Pyrosequencing

Starting at $171.00 per sample

New Project Set-up Fee (includes consultation, primer and assay design) $163

Bisulfite Conversion (Qiagen Protocol) $13

PCR and Pyrosequencing Run/Sample (one target region) $15

Pyrosequencing Run Only $8

New Project Set-up Fee (includes consultation, primer and assay design) $163

Bisulfite Conversion (Qiagen Protocol) $13

PCR and Pyrosequencing Run/Sample (one target region) $15

Pyrosequencing Run Only $8

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SNP Genotyping Data Analysis

Single nucelotide polymorphism genotyping data analysis
Price on request
Request a quote for more information about this service.

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Surface Plasmon Resonance (SPR)

Starting at $724.00 per sample

Set-up fee (per chip, user provides chip) $274

Experiment (full-day duration) $450

Experiment (half-day duration) $225

User-operated instrument session (per hour) $67

User-operated instrument session (per day) $302

Consulting or data analysis (half-day duration) $219

Set-up fee (per chip, user provides chip) $274

Experiment (full-day duration) $450

Experiment (half-day duration) $225

User-operated instrument session (per hour) $67

User-operated instrument session (per day) $302

Consulting or data analysis (half-day duration) $219

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Tiling Array

Starting at $282.00 per sample

Full Service: High-density tiled microarrays for transcript mapping and chromatin immunoprecipitation human, mouse, yeast

Only one array:
Starting material: DNA $282 per sample
Starting material: RNA $575 per sample

One set (7 arrays):
Starting material: DNA $866 per sample
Starting material: RNA $1108 per sample

Full Service: High-density tiled microarrays for transcript mapping and chromatin immunoprecipitation human, mouse, yeast

Only one array:
Starting material: DNA $282 per sample
Starting material: RNA $575 per sample

One set (7 arrays):
Starting material: DNA $866 per sample
Starting material: RNA $1108 per sample

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Whole Genome Sequencing

Starting at $472.00 per sample

Full GeneChip mapping service
Starting material: genomic DNA

10K XbaI inquire

50K Xba or Hind inquire

100K Xba and Hind inquire

250K Nsp or Sty inquire

500K Nsp and Sty (2 chips) inquire

Human SNP Array 5.0 (1 chip) $472

Human SNP Array 6.0 (1 chip) $510

Full GeneChip mapping service
Starting material: genomic DNA

10K XbaI inquire

50K Xba or Hind inquire

100K Xba and Hind inquire

250K Nsp or Sty inquire

500K Nsp and Sty (2 chips) inquire

Human SNP Array 5.0 (1 chip) $472

Human SNP Array 6.0 (1 chip) $510

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qPCR

Quantitative PCR
Starting at $25.00 per sample

Instrument run: (per plate)
Client prepares plate ready to run $25

Full Service set-up fee per order: (Prices do not include primers) $166

Taqman Chemistry
Reverse Transcription (per sample) $20
Taqman qPCR per sample (triplicate) $10
Custom Assay - Taqman qPCR optimization

SybrGreen Chemistry
Reverse Transcription... Show more »

Instrument run: (per plate)
Client prepares plate ready to run $25

Full Service set-up fee per order: (Prices do not include primers) $166

Taqman Chemistry
Reverse Transcription (per sample) $20
Taqman qPCR per sample (triplicate) $10
Custom Assay - Taqman qPCR optimization

SybrGreen Chemistry
Reverse Transcription (per sample) $12
SybrGreen qPCR per sample (triplicate) $10
Custom Assay - SybrGreen qPCR optimization

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RNA Microarray Platforms

Price on request
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Therapeutic Monoclonal Antibody (mAb) Development

Price on request
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Biochemistry & Molecular Biology

Price on request
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Imaging & Spectroscopy

Price on request
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PCR

Price on request
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Chemistry and Materials

Price on request
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MicroRNA (miRNA) Microarray

Price on request
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DNA Hybridization

Price on request
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Nucleic Acid Data Analysis

Price on request
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Synthetic Chemistry

Price on request
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Next Generation Sequencing (NGS)

Price on request
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Nucleic Acid Services

Price on request
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RNA Microarray

Price on request
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MALDI Mass Spectrometry Methods

Price on request
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DNA Services

Price on request
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RNA Services

Price on request
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Protein Sequencing

Price on request
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Computational Modeling

Price on request
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Biology

Price on request
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Biostatistics & Bioinformatics

Price on request
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Antibody Services

Price on request
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Biomolecular Interaction Analysis

Price on request
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DNA Microarrays

Price on request
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Protein Services

Price on request
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Spectroscopy

Price on request
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Sequencing Data Analysis and Management

Price on request
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Equipment

Price on request
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DNA Synthesis and Probe Development

Price on request
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Nucleic Acid Analysis

Price on request
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Mass Spectrometry

Price on request
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