1379046072341 d4243b2d6ca86e479e25a1746e1ac940

ProNovus Bioscience, LLC

75 Orders Completed
Mountain View, California, US

About ProNovus Bioscience, LLC

Type: Privately Held Size: 1-10 employees

ProNovus Bioscience is an established CRO specializing in stable cell line generation, robust protein expression, and thorough protein characterization (even for challenging membrane proteins). Experienced bench scientists with over 30 years experience in academic and industry research, our founders have worked... Show more »

ProNovus Bioscience is an established CRO specializing in stable cell line generation, robust protein expression, and thorough protein characterization (even for challenging membrane proteins). Experienced bench scientists with over 30 years experience in academic and industry research, our founders have worked in top biotech and biopharma institutions in the San Francisco, CA Bay Area. They are known for providing a wide range of scientific support, technical development and bio-reagents development in applied molecular biology and membrane protein biology (especially for GPCRs and Transporters) to research teams all over the world. Collectively, our scientists have been key contributors for numerous publications in peer-reviewed journals including Nature and Cell.

In addition, check out publications where our services were used:

  1. 2018: "Proinflammatory Cytokines, Mood, and Sleep in Interepisode Bipolar Disorder and Insomnia: A Pilot Study With Implications for Psychosocial Interventions" (https://journals.lww.com/psychosomaticmedicine/Abstract/2018/01000/Proinflammatory_Cytokines,_Mood,_and_Sleep_in.12.aspx)

  2. 2018: "Neuronal Preconditioning Requires the Mitophagic Activity of C-terminus of HSC70-Interacting Protein" (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6070662/)

  3. 2018: "Syntaxins on granules promote docking of granules via interactions with munc18" (https://www.nature.com/articles/s41598-017-18597-z)

  4. 2018: "Localized gene expression changes during adventitious root formation in black walnut (Juglans nigra L.)" (https://htirc.org/wp-content/uploads/2018/05/Stevens-Woeste-and-Pijut-Tree-Physiology-2018.pdf)

  5. 2016: "Nonsense Mutations in the Rhodopsin Gene that Give Rise to Mild Phenotypes Trigger mRNA Degradation in Human Cells by Nonsense-Mediated Decay" (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4808503/)

  6. 2016: "Transcriptomic profiling of curcumin-treated human breast stem cells identifies a role for stearoyl-coa desaturase in breast cancer prevention" (https://link.springer.com/article/10.1007%2Fs10

And publications where ProNovus scientists are contributing authors:

  1. 2017: "Replication Study: Inhibition of BET recruitment to chromatin as an effective treatment for MLL-fusion leukaemia" (https://elifesciences.org/articles/25306)

  2. 2015: "Registered report: androgen receptor splice variants determine taxane sensitivity in prostate cancer" (https://peerj.com/articles/1232/)

  3. 2015: "Registered report: Inhibition of BET recruitment to chromatin as an effective treatment for MLL-fusion leukemia" (https://elifesciences.org/articles/08997)

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Our Services (53)


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Biopharmaceutical Consulting

Price on request

We have over 30+ years combined experience in drug discovery, encompassing small molecules and biologics, as well as spanning a wide array of targets and diseases. 

We have over 30+ years combined experience in drug discovery, encompassing small molecules and biologics, as well as spanning a wide array of targets and diseases. 

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Bioconjugation/Chemical Crosslinking

Price on request

Our scientists have a wealth of experience in site-specifically labeling proteins and antibodies with fluorophores for biophysical studies and detection (Alexa Fluors, DyLights, R-PE, APC, etc.). In addition, we provide other forms of labeling (e.g. biotinylation) of antibodies and proteins.

Our scientists have a wealth of experience in site-specifically labeling proteins and antibodies with fluorophores for biophysical studies and detection (Alexa Fluors, DyLights, R-PE, APC, etc.). In addition, we provide other forms of labeling (e.g. biotinylation) of antibodies and proteins.

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ABI 7700 Opticon 2

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Hybridoma Production

Price on request

Using your existing hybridoma, our scientists expand, culture and typically generate hybridoma production runs of milligrams per liter. We also have excellent capabilities in purification of your antibody as well as downstream antibody modifications.

Typical turnaround time is 2-4 weeks, depending on request and scale.

Using your existing hybridoma, our scientists expand, culture and typically generate hybridoma production runs of milligrams per liter. We also have excellent capabilities in purification of your antibody as well as downstream antibody modifications.

Typical turnaround time is 2-4 weeks, depending on request and scale.

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Immuno-Oncology Drug Discovery

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ELISA

Enzyme-linked immunosorbent assay
Starting at $350.00 per plate

We specialize in colorimetric ELISAs. We can work with your specific protocol (using your antigens and/or antibodies) or with commercially available kits. We've worked with both industry and academic partners, with our results included in some publications.

Prices start at $195, depending on number of samples and if kits are provided by customer.

We specialize in colorimetric ELISAs. We can work with your specific protocol (using your antigens and/or antibodies) or with commercially available kits. We've worked with both industry and academic partners, with our results included in some publications.

Prices start at $195, depending on number of samples and if kits are provided by customer.

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Biomarker Analysis

Price on request
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Biomarkers

Price on request
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qPCR

Quantitative PCR
Price on request

We offer a full range of services for qPCR studies from RNA isolation to Data Analysis. We are equipped to perform both SYBR and Probe-based qPCR assays. Depending on experimental needs, we can perform data analysis using either the standard curve method or relative expression (ddCt). We have endogenous controls for most... Show more »

We offer a full range of services for qPCR studies from RNA isolation to Data Analysis. We are equipped to perform both SYBR and Probe-based qPCR assays. Depending on experimental needs, we can perform data analysis using either the standard curve method or relative expression (ddCt). We have endogenous controls for most commonly used pre-clinical species including canine.

· RNA (One-step) and cDNA Preparation (Two-step)
· Primer and Probe Design
· Experimental Design
· qPCR Run
· Data Analysis

Please contact us for additional information, experimental consultation, or a quote.

Pricing as low as $20 per sample.

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ABI 7700 Opticon 2 ABI 7900HT

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Assay Development

Starting at $150.00 per hour

We have years of expertise in HTS assay development and screening design. Our general area of specialty is membrane proteins: GPCRs and ABC and SLC transporters. We work with whole mammalian cells, whole insect cells or membranes of those species. Instrumentation includes absorbance, fluorescence and luminescence readers, with... Show more »

We have years of expertise in HTS assay development and screening design. Our general area of specialty is membrane proteins: GPCRs and ABC and SLC transporters. We work with whole mammalian cells, whole insect cells or membranes of those species. Instrumentation includes absorbance, fluorescence and luminescence readers, with further capabilities in TR-FRET, FRET, BRET.

We currently carry insect cell lines (Sf9, Tni-High Five cells) and various E.coli bacterial strains.

For mammalian cell lines, we currently can process (i.e. purify from or prepare fractions from), develop and maintain the cell lines.

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Northern Blot

Price on request

We provide Northern blot services using non-radioactive, chemiluminescent detection. The customer can provide sufficient amounts of total RNA or mRNA or we can perform the RNA extraction/mRNA extraction. Upon completion of the project, we will provide the customer with digital images of the Northern blot. Pricing will be determined by the amount of samples as well of the scope of the project.

We provide Northern blot services using non-radioactive, chemiluminescent detection. The customer can provide sufficient amounts of total RNA or mRNA or we can perform the RNA extraction/mRNA extraction. Upon completion of the project, we will provide the customer with digital images of the Northern blot. Pricing will be determined by the amount of samples as well of the scope of the project.

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Membrane Protein Identification

Price on request
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RT-PCR

Reverse transcription polymerase chain reaction
Price on request
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ABI 7700 Opticon 2

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PyMOL Molecular Visualization

Price on request

We have a wealth of experience generating 3D models of proteins useful for publications or for research purposes to help you visualize results and form/test hypothesis. Contact us for more information!

We have a wealth of experience generating 3D models of proteins useful for publications or for research purposes to help you visualize results and form/test hypothesis. Contact us for more information!

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In silico Pharmacological Modeling

Price on request
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Protein Modeling

Price on request
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Bradford Assay

Starting at $40.00 per sample

Protein quantification using BSA as standard. 4 samples per set. Aside from a standard Bradford assay, we also offer BCA protein assay and 660nm protein assay capabilities.

Protein quantification using BSA as standard. 4 samples per set. Aside from a standard Bradford assay, we also offer BCA protein assay and 660nm protein assay capabilities.

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FRET

Fluorescence resonance energy transfer
Starting at $100.00 per hour

We specialize in membrane protein FRET experiments to assess interaction and oligomerization. We have capabilities in performing these studies in a biochemical high-throughput format. We also offer consultation on experimental design (FRET pairs, protein tagging, etc).

Prices will vary greatly depending on scale, assay system, desired deliverable.

We specialize in membrane protein FRET experiments to assess interaction and oligomerization. We have capabilities in performing these studies in a biochemical high-throughput format. We also offer consultation on experimental design (FRET pairs, protein tagging, etc).

Prices will vary greatly depending on scale, assay system, desired deliverable.

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qRT-PCR

Quantitative reverse transcription polymerase chain reaction
Price on request

We offer a full range of services for qPCR studies from RNA isolation to Data Analysis. We are equipped to perform both SYBR and Probe-based qPCR assays. Depending on experimental needs, we can perform data analysis using either the standard curve method or relative expression (DDCt). We have endogenous controls for most... Show more »

We offer a full range of services for qPCR studies from RNA isolation to Data Analysis. We are equipped to perform both SYBR and Probe-based qPCR assays. Depending on experimental needs, we can perform data analysis using either the standard curve method or relative expression (DDCt). We have endogenous controls for most commonly used pre-clinical species including canine.

· RNA (One-step) and cDNA Preparation (Two-step)
· Primer and Probe Design
· Experimental Design
· qPCR Run
· Data Analysis

Please contact us for additional information, experimental consultation, or a quote.

Pricing as low as $20.

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ABI 7700 Opticon 2 ABI 7900HT

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Luciferase Assays

Price on request
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Plasmid DNA Preparation

Starting at $30.00 per sample

Plasmid DNA extraction and purification using mini-, midi-, maxi- and -giga columns.

Pricing will be determined by the number of samples requested and by the scale of purification.

Customer will provide plasmid or bacterial colony and information about the plasmid (eg. low/high copy plasmid, antibiotic resistance, other... Show more »

Plasmid DNA extraction and purification using mini-, midi-, maxi- and -giga columns.

Pricing will be determined by the number of samples requested and by the scale of purification.

Customer will provide plasmid or bacterial colony and information about the plasmid (eg. low/high copy plasmid, antibiotic resistance, other special media needs).

We will provide prepped plasmid with DNA concentration, O.D.260/280 ratio and agarose gel. For additional cost, we can also sequence verify your plasmid.

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Western Blot

Starting at $250.00 per gel

Capabilities:

  • Protein concentration determination (BSA method)
  • Strip/reprobe membranes
  • Optimize protocols for particular antigen/target

Visualization:

  • Chemiluminescent or chromogenic substrate on imager

Number of samples in a single run: 10-12

Capabilities:

  • Protein concentration determination (BSA method)
  • Strip/reprobe membranes
  • Optimize protocols for particular antigen/target

Visualization:

  • Chemiluminescent or chromogenic substrate on imager

Number of samples in a single run: 10-12

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TaqMan Assays

TaqMan Gene Expression Assays
Price on request

We offer a full range of services for qPCR studies from RNA isolation to Data Analysis. We are equipped to perform both SYBR and Probe-based qPCR assays (Taqman). Depending on experimental needs, we can perform data analysis using either the standard curve method or relative expression (ddCt). We have endogenous controls for... Show more »

We offer a full range of services for qPCR studies from RNA isolation to Data Analysis. We are equipped to perform both SYBR and Probe-based qPCR assays (Taqman). Depending on experimental needs, we can perform data analysis using either the standard curve method or relative expression (ddCt). We have endogenous controls for most commonly used pre-clinical species including canine.

· RNA (One-step) and cDNA Preparation (Two-step)
· Primer and Probe Design
· Experimental Design
· qPCR Run
· Data Analysis

Please contact us for additional information, experimental consultation, or a quote.

Pricing as low as $20 per sample.

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ABI 7700 Opticon 2 ABI 7900HT

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Site-Directed Mutagenesis

Starting at $350.00 per sample

Our site directed mutagenesis services include performing single point mutations, insertions, or deletions.

You provide us with 1-2 ug of plasmid, sequence, and antibiotic resistance information.

Our service can include all or partial steps (please inquire about prices):

-Mutagenesis primer design and order
-Mutagenesis... Show more »

Our site directed mutagenesis services include performing single point mutations, insertions, or deletions.

You provide us with 1-2 ug of plasmid, sequence, and antibiotic resistance information.

Our service can include all or partial steps (please inquire about prices):

-Mutagenesis primer design and order
-Mutagenesis by PCR or Quikchange
-Sequence verification
-Subcloning into new or other vector

Delivery of purified mutated plasmid DNA, glycerol stock, and sequence data.

As low as $300 per sample.

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RNA Extraction and Purification

Price on request

We have extensive experience in RNA extraction from a number of sample sources such as frozen tissue biopsies, tissue culture cells, blood, etc. that can be used for downstream processes such as cDNA synthesis, qPCR, or Northern blots. Please inquire about special handling of samples prior to submission to our facility.

We have extensive experience in RNA extraction from a number of sample sources such as frozen tissue biopsies, tissue culture cells, blood, etc. that can be used for downstream processes such as cDNA synthesis, qPCR, or Northern blots. Please inquire about special handling of samples prior to submission to our facility.

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qPCR Data Analysis

Quantitative PCR Data Analysis
Price on request
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ABI 7700 Opticon 2 ABI 7900HT

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qPCR Assay Design

Quantitative PCR experimental design
Price on request
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Protein Production

Price on request
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RNA Services

Price on request
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Computational Modeling

Price on request
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Biochemical Assays

Price on request
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ADME/DMPK Studies

Drug Metabolism and Pharmacokinetics
Price on request
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Nucleic Acid Services

Price on request
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DNA Services

Price on request
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In vitro Reporter Gene Assays

Price on request
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Pharmacology & Toxicology

Price on request
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Cytotoxicity Assays

Starting at $950.00 per sample

Determination of growth inhibition using an MTS cell proliferation assay, typically using Promega CellTiter-Aqueous One, unless otherwise requested. We will test an 8 point curve of your compound and determine IC50 values. Price is per compound

Determination of growth inhibition using an MTS cell proliferation assay, typically using Promega CellTiter-Aqueous One, unless otherwise requested. We will test an 8 point curve of your compound and determine IC50 values. Price is per compound

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Immunology Oncology Drug Discovery

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Cells and Tissues

Price on request
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Receptor Assays

Price on request
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Protein Quantification

Price on request
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Antibody Services

Price on request
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Cell-Based Screening Methods

Price on request
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Toxicology

Price on request
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Project Management

Price on request
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Protein Expression Visualization

Price on request
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Imaging & Spectroscopy

Price on request
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Biology

Price on request
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Cell-Based Assays

Price on request
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Biochemistry & Molecular Biology

Price on request
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Plasmids and Vectors

Price on request
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Structural Biology

Price on request
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Drug Discovery & Development

Price on request

Drug Discovery & Development Services

Drug Discovery & Development Services

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FPLC

Fast Performance liquid chromatography
Price on request
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Protein Database Searching

Price on request
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Pharmacology

Price on request

Pharmacology Services

Pharmacology Services

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Missing Avatar

J.J. Fung

Director of Protein Sciences R&D, Co-Founder
Missing Avatar

Alan Kosaka

Senior Scientist, Co-Founder

FileType/PDF Created with Sketch. Capability-PNB-2019.pdf

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  • Positive review for ELISA:

    November 2017

    "The ProNovus team was quick to respond to my ELISA request. Everything went smoothly, and the results were presented to me in an organized and timely fashion. I will continue to work with ProNovus."

  • Positive review for ELISA:

    August 2017

    "ProNovus helped with an ELISA experiment and generated clear, informative results in a very short turn around time. It was easy to communicate with the ProNovus team throughout the project, for both the scientific/technical and billing/logistic aspects. I found their team professional and knowledgeable. I would work with ProNovus again without hesitation."

  • Positive review for Molecular Cloning:

    October 2016

    "I was very happy with the outcomes of working with Dr. Kosaka and ProNovus. The project that I was requesting was a little bit different to a standard procedure, and Dr. Kosaka was very helpful in suggesting a novel approach that was very successful. He also gave some good general advice, and completed the work successfully within the turnaround time promised. I would recommend ProNovus."

  • Positive review for Protein Purification:

    October 2015

    "Had a great experience working on a project with Dave. Communication and expectations were clear, reasonable and realistic. This allowed us to have an effective collaboration."

  • Positive review for Site-Directed Mutagenesis:

    April 2015

    "I've used Pronovus Bioscience services twice. They are professional, fast, efficient and responsive. They've got the projects completed perfectly on time with no issues. I highly recommend this company."

  • Positive review for Bradford Assay:

    September 2014

    "Quick work, and even provided a workaround to an initially unforeseen problem."

  • Missing Avatar

    Santosh SuryavanshiFebruary 2016

    I came to know about ProNovus through scienceexchange and I started custom mutagenesis project with Alan Kosaka on Jan 21, 2016. They finished making three mutant plasmids in 3 weeks while other three companies I tried last 2-3 months failed to do so. I highly endorse this lab if you need your custom mutagenesis work to be done quickly.

  • Missing Avatar

    Maria BorisovskaApril 2015

    Pronovus Bioscience has competed 2 cloning projects for me. Both were done quickly and for a very reasonable cost. I would highly recommend this company.

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