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PrimBio Research Institute LLC

Exton, Pennsylvania, US

PrimBio Research Institute uses the Ion Torrent gene sequencing platform to provide genomic sequencing services. We are also researching and developing targeted gene panels for diseases diagnosis, prevention and treatment.

Certifications: Certified Life Technologies Service Providers

Instrumentation: Ion Torrent & Ion Proton Sequencer

PrimBio Research Institute LLC has not listed any services.

Lung Cancer Gene Panel
Price on request

Designed with the OncoNetwork consortium, the Ion AmpliSeq™ Colon and Lung Cancer Panel contains primer pairs that analyze hotspot regions of 22 genes implicated in colon and lung cancers. The OncoNetwork consortium comprises eight cancer researchers from different translational research institutions with many years of experience... Show more »

Designed with the OncoNetwork consortium, the Ion AmpliSeq™ Colon and Lung Cancer Panel contains primer pairs that analyze hotspot regions of 22 genes implicated in colon and lung cancers. The OncoNetwork consortium comprises eight cancer researchers from different translational research institutions with many years of experience in adopting the latest DNA sequencing and genotyping technologies to pioneer colon and lung therapy research.

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Ion AmpliSeq
Colon Cancer Gene Panel
Price on request

Designed with the OncoNetwork consortium, the Ion AmpliSeq™ Colon and Lung Cancer Panel contains primer pairs that analyze hotspot regions of 22 genes implicated in colon and lung cancers. The OncoNetwork consortium comprises eight cancer researchers from different translational research institutions with many years of experience... Show more »

Designed with the OncoNetwork consortium, the Ion AmpliSeq™ Colon and Lung Cancer Panel contains primer pairs that analyze hotspot regions of 22 genes implicated in colon and lung cancers. The OncoNetwork consortium comprises eight cancer researchers from different translational research institutions with many years of experience in adopting the latest DNA sequencing and genotyping technologies to pioneer colon and lung therapy research.

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Ion AmpliSeq
BRCA Mutation Analysis
Price on request

Mutations in tumor suppressor genes BRCA1 and BRCA2 have been implicated in hereditary breast and ovarian cancer. Much research is currently being done today to better detect, treat, and prevent cancer by assessing mutations in those who carry mutations in BRCA1 and BRCA2. The PrimBio BRCA1/2 Panel utilizes 167 amplicons to... Show more »

Mutations in tumor suppressor genes BRCA1 and BRCA2 have been implicated in hereditary breast and ovarian cancer. Much research is currently being done today to better detect, treat, and prevent cancer by assessing mutations in those who carry mutations in BRCA1 and BRCA2. The PrimBio BRCA1/2 Panel utilizes 167 amplicons to analyze the coding regions of both BRCA1 and BRCA2.
This panel was designed and verified with input from leading cancer researchers from two institutes and is now being further verified by an additional 5 laboratories. These two groups verified the performance of the panel on 65 unique samples that were previously screened using orthogonal technologies. They combined the potential of Ion AmpliSeq and Ion semiconductor technology to develop a variant screening solution that meets these goals:
• High coverage of the target region-expanded targeted regions to include the entire coding region, including 10-20 bases of padding around all targeted coding exons. Amplicons are designed to overlap for sequence coverage redundancy
• High-performance amplicons and uniformity-stringent primer design ensures that primers do not overlap and are not located in regions with high-frequency SNPs
• Fast turnaround time coupled with throughput flexibility-enabling rapid time-to-results in processing either a small or large number of samples
In addition to these goals the PrimBio BRCA1 and BRCA2 Panel has unmatched amplicon coverage such that it is:
• Designed for 100% amplicon coverage of all targeted coding exons and exon-intron boundaries
• Expanded target regions-amplicons were designed to cover 10-20 bases beyond the targeted coding exon and exon-intron boundaries
• Created for sequence coverage redundancy with overlapping amplicons across exons
• High-fidelity primers
• No SNPs with frequency >.5% are included in the last 5 nucleotides of the primers
• Only SNPs with a population frequency <.5% are present at other positions

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16S rRNA Sequencing
Price on request

The Ion 16S™ Metagenomics Kit is designed for rapid, comprehensive, and broad-range research analyses of mixed microbial populations. The kit uses two primer pools to amplify seven hypervariable regions (V2, V3, V4, V6, V7, V8, and V9) of bacterial 16S rRNA. The combination of the two primer pools enables broad-range, sequence-based identification of bacteria from complex mixed populations.

The Ion 16S™ Metagenomics Kit is designed for rapid, comprehensive, and broad-range research analyses of mixed microbial populations. The kit uses two primer pools to amplify seven hypervariable regions (V2, V3, V4, V6, V7, V8, and V9) of bacterial 16S rRNA. The combination of the two primer pools enables broad-range, sequence-based identification of bacteria from complex mixed populations.

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Targeted Cancer Gene Panel
Starting at $500.00 per sample

The Ion AmpliSeq™ Cancer Hotspot Panel v2 allows translational and disease researchers to fast-track oncology research by surveying hotspot regions of 50 oncogenes and tumor suppressor genes, with wide coverage of the KRAS, BRAF, and EGFR genes.

This panel contains 207 primer pairs and requires only 10 ng of DNA, enabling researchers to sequence challenging samples like FFPE tissue.

The Ion AmpliSeq™ Cancer Hotspot Panel v2 allows translational and disease researchers to fast-track oncology research by surveying hotspot regions of 50 oncogenes and tumor suppressor genes, with wide coverage of the KRAS, BRAF, and EGFR genes.

This panel contains 207 primer pairs and requires only 10 ng of DNA, enabling researchers to sequence challenging samples like FFPE tissue.

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Whole Exome Sequencing (WES)
Price on request

The Ion AmpliSeq™ Exome Kit enables the fastest and simplest method of exome enrichment to accelerate your human disease research, producing exome libraries in under 6 hours with less than 60 minutes of hands-on time. Leveraging the ultra-high multiplex PCR approach of Ion AmpliSeq™ technology with the Ion Proton™ Sequencer, the... Show more »

The Ion AmpliSeq™ Exome Kit enables the fastest and simplest method of exome enrichment to accelerate your human disease research, producing exome libraries in under 6 hours with less than 60 minutes of hands-on time. Leveraging the ultra-high multiplex PCR approach of Ion AmpliSeq™ technology with the Ion Proton™ Sequencer, the Ion AmpliSeq™ Exome Kit allows for rapid sequencing of key exonic regions of the genome, going from DNA to variants in just 2 days.

The Ion AmpliSeq™ Exome Kit enable you to:

• Simplify exome enrichment through the combination of PCR and the Ion AmpliSeq™ workflow, resulting in less than 1 hour of hands-on time to produce exome libraries in under 6 hours
• Accelerate your disease research with rapid exome sequencing, going from DNA to variants in just 2 days
• Access affordable exome sequencing when you need it, without the need to batch dozens of samples, or the high price of a lower-throughput run
• Maximize sequencing efficiency with >90% on-target bases and >92% coverage uniformity and design covering >97% of the Consensus Coding Sequences (CCDS) annotation

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Customized Gene Panels
Price on request

We design custom gene panels according to the projects. The price varies depending on the number of ampicons. Please contact us for more information.

We design custom gene panels according to the projects. The price varies depending on the number of ampicons. Please contact us for more information.

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Inherited Disease Gene Panel
Price on request

The Ion AmpliSeq™ Inherited Disease Panel comprises over 10,000 primer pairs that target genes involved in some of the most common Mendelian diseases, making it ideal for a broad survey of inherited disease genes.
Based on the transformative Ion AmpliSeq™ technology that enables the selective amplification of tens to thousands of... Show more »

The Ion AmpliSeq™ Inherited Disease Panel comprises over 10,000 primer pairs that target genes involved in some of the most common Mendelian diseases, making it ideal for a broad survey of inherited disease genes.
Based on the transformative Ion AmpliSeq™ technology that enables the selective amplification of tens to thousands of target sequences in a single multiplex
PCR reaction, the Ion AmpliSeq™ Inherited Disease Panel employs more than 10,000 primer pairs in just 3 pools to amplify the coding exons of over 300 genes
associated with over 700 unique inherited diseases, including neuromuscular, cardiovascular, developmental, and metabolic diseases.
Developed to match genes targeted by clinical molecular geneticists studying inherited diseases, including genes listed in the NIH Genetic Test Registry, the Ion AmpliSeq™ Inherited Disease Panel is designed to deliver unrivaled genomic scope with simplicity, scalability, and speed.

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Comprehensive Cancer Gene Panel
Price on request

Ion Ampliseq™ Comprehensive Cancer Panel facilitates broad oncology surveys using only 40 ng of DNA with ~16,000 primer pairs multiplexed into just 4 pools.
Working with key cancer researchers and incorporating information from literature and database searches, Ion Torrent designed the Ion AmpliSeq™ Comprehensive
Cancer Panel... Show more »

Ion Ampliseq™ Comprehensive Cancer Panel facilitates broad oncology surveys using only 40 ng of DNA with ~16,000 primer pairs multiplexed into just 4 pools.
Working with key cancer researchers and incorporating information from literature and database searches, Ion Torrent designed the Ion AmpliSeq™ Comprehensive
Cancer Panel to target the exons of tumor suppressor genes and oncogenes frequently cited and frequently mutated. The Ion AmpliSeq™ Comprehensive Cancer
Panel is strategically designed to simultaneously interrogate coding DNA sequences (CDS) and splice variants across multiple gene families. This pathway-based gene selection profiles the mutational spectrum in cancer driver genes and drug targets along with signaling cascades, apoptosis genes, DNA repair genes, transcription regulators, inflammatory response genes, and growth factor genes, all in a single assay. Additionally, this panel targets the 50 genes selected in
the focused Ion AmpliSeq™ Cancer Panel v2. Ion AmpliSeq™Comprehensive Cancer Panel allows researchers to fast-track projects by eliminating time-consuming and labor-intensive primer design and target selection procedures, while targeting an extensive set of oncology genes.After conducting broad variant surveys, researchers may choose to conduct in-depth somatic variant analysis of a subset of genes from this panel. Using the Ion AmpliSeq™ Designer, an online tool for creating and ordering custom panels, we can
simply enter the selected genes and create a custom cancer gene panel for our customers to assist for them for further study.

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RNA Sequencing
Starting at $510.00 per sample

RNA sequencing (Ion Proton sequencer)
Analysis: Not included
Turn around time: 2 weeks

We provide RNAseq services with up to 80 million reads per sample. We can only provide single-ended read. Bioinformatics can be provided upon request. We also provide small RNAseq and guarantee the quality of our work.

All RNA... Show more »

RNA sequencing (Ion Proton sequencer)
Analysis: Not included
Turn around time: 2 weeks

We provide RNAseq services with up to 80 million reads per sample. We can only provide single-ended read. Bioinformatics can be provided upon request. We also provide small RNAseq and guarantee the quality of our work.

All RNA sequencing services include mRNA isolation, library preparation, sequencing, and alignment.

Required from researcher: 2-10 µg of total RNA for RNAseq and 1x10e7 cells for small RNAseq for all types of species

Deliverables: bioinformatics available upon request

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qRT-PCR
Quantitative reverse transcription polymerase chain reaction
Price on request

TaqMan Real time PCR assays using QuantStudio 12k.

TaqMan Real time PCR assays using QuantStudio 12k.

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Breast Cancer Gene Panel
Price on request

This panel targets breast cancer related genes, including BRCA1/2, other candidate genes for inherited breast cancers, and susceptible genes that may have sporadic mutations in breast cancer. With complete exon coverage of 31 genes, this panel delivers fast, FFPE-compatible, target selection for a broad survey of key breast cancer related genes.

This panel targets breast cancer related genes, including BRCA1/2, other candidate genes for inherited breast cancers, and susceptible genes that may have sporadic mutations in breast cancer. With complete exon coverage of 31 genes, this panel delivers fast, FFPE-compatible, target selection for a broad survey of key breast cancer related genes.

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Colorectal Cancer Gene Panel
Price on request

PrimBio Colorectal Cancer Panel is a NGS panel that detects mutations in 48 genes associated with increased risk for colorectal cancer. Genes on this panel include APC, AKT1, MLH1, MSH2, MSH6, TP53, and more. The sequences of the exons and UTRs of these genes are analyzed to find clinically relevant variants.

PrimBio Colorectal Cancer Panel is a NGS panel that detects mutations in 48 genes associated with increased risk for colorectal cancer. Genes on this panel include APC, AKT1, MLH1, MSH2, MSH6, TP53, and more. The sequences of the exons and UTRs of these genes are analyzed to find clinically relevant variants.

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Human
Ion Torrent Ion Proton
Clinical Research
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Clinical Laboratory Services
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Clinical Molecular Diagnostics
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Gene Panel Testing
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Genetic Marker Screening Services
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Biology
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Biochemistry & Molecular Biology
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Nucleic Acid Services
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DNA Services
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DNA Sequencing
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Next Generation Sequencing (NGS)
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Targeted Gene Sequencing
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Exome Sequencing Services
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PCR
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RNA Services
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2017-02-23 12:16:31 -0600

Net Promoter Score of 10 received for 16S rRNA Sequencing.

Additional Ratings: satisfaction with deliverable: 8, satisfaction with timeliness: 10.
2016-09-28 19:37:45 -0500

Net Promoter Score of 9 received for Ion Torrent Sequencing.

Additional Ratings: satisfaction with deliverable: 9, satisfaction with timeliness: 10.
September 11, 2014

Positive review received for RNA Sequencing:

"Great project with 56 RNAseq samples. Results were fast delivered in good quality. Good scientific communication with Diana."

August 27, 2014

Positive review received for RNA Sequencing:

"Again a good project done by PrimBIo. Good and fast communication."

May 20, 2014

Positive review received for RNA Sequencing:

"Quick sequencing and good customer service!"

January 28, 2014

Positive review received for RNA Sequencing:

"Very good and fast service. Also our research questions were accurately handled. This was our first IonProton run for RNAseq, and worked out very well. Highly recommended!"

October 22, 2013

Positive review received for Whole Exome Sequencing (WES):

"Excellent work, will use again."

July 12, 2013

Positive review received for RNA Sequencing:

"Simply great!"

March 28, 2013

Positive review received for Ion Torrent Sequencing:

"The service was superb. Very fast and very efficient. Thank you."

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