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Premas Biotech

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Gurgaon, IN

About Premas Biotech

Founded: 2005 Type: Privately Held Size: 51-200 employees

Premas aspires to accelerate the delivery of new medicines to patients through building partnerships with biotech and pharmaceutical companies around the world. Our mission is to continuously make it sure, secure, faster, and cheaper for therapeutic pharmaceutical companies to develop novel solutions.

Premas Biotech was founded in 2005 with the vision of transforming drug development. We have acquired in-depth expertise and built state-of-the-art technology platforms to provide quality products that are second to none.

Premas employs a world-class team of more than 150 dedicated scientists and managers, who have successfully developed over 650 molecules. By fostering an innovative and inclusive culture, we have created an ecosystem for establishing productive, long-term partnerships with our clients.

We aim to be problem-solvers for our customers, by actively listening and being accessible 24/7 wherever we are needed. We innovate based on our customers’ needs. Our organization has the flexibility to adapt the way we do business to what each customer needs, supporting the development activities for companies of all sizes.

Our Services (21)


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Cell-Based Assays

Price on request
  • Proliferation
  • Apoptosis
  • Ex vivo cell viability
  • Cytotoxicity
  • Reporter gene (Luminescence)
  • Receptor- ligand binding & uptake (Fluorescence/ Luminescence)
  • Cytokine release (ELISA)


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Ligand Binding Assays

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Cytotoxicity Assays

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Protein Characterization

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Premas performs and develops analytical techniques for in-process testing, end-product analysis and formulation / stability studies to deliver a high quality, active protein.

S. No. Characterization Processes
1 Purity Analysis SDS-PAGE , HPLC , Mass-spectrometry
2 Quantity Analysis Densitometry, Protein Estimation by Bradford method, Lowry and BCA method.
3 Identity Analysis Qualitative ELISA, Western Blot ,MALDI, Peptide Mapping
4 Contaminant Analysis Host Cell DNA, Host Cell Protein, Endotoxin Profile, HPLC
5 Aggregation Analysis DLS Method
6 Functionality Study Biochemical assays, Cell based Assays, SPR (Surface Plasmon Resonance)
7 Stability Analysis Lyophilization Study, Temperature Study, Buffer Compositions


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Protein Purification

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We possess the expertise and resources to purify from milligrams to grams of proteins and develop processes for purification of proteins from various sources. We have particularly developed a variety of protocols for isolation of insoluble proteins, on column refolding strategies, as well as membrane proteins. We deliver biologically active, pure and stable protein by choosing an efficient capture strategy and develop a robust purification process with minimal variation.

Our Services Include

  • Protein purification using client-supplied protocols, standard protocols and customized protocols according to client's requirements.
  • Protein purification method development for transfer to a GMP facility mg to gm to kg level scale up.
  • Protein purification up to 99% purity.
  • Optimization and development of purification method using different chromatographic techniques
    • Affinity
    • Ion Exchange
    • Size Exclusion
    • Reverse Phase
    • Hydrophobic Interaction
  • Purification of recombinant tagged proteins (His6, GST, MBP, SUMO, FLAG, etc.) plus option of tag removal by proteolytic digestion (Thrombin, TEV, PreScission, SUMO protease, Factor Xa, etc.)
  • Refolding and purification of insoluble proteins from inclusion bodies.
    • Solubilization and refolding studies.
  • Optimizing storage conditions for various downstream applications.


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Molecular Cloning

Price on request
  • Design and synthesis of vectors based on your research needs or a vector of your choice.
  • PCR cloning and subcloning services from customer provided templates.


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In Silico Protein Sequence Analysis

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One of the very first steps in the development of biopharmaceuticals or production of any recombinant protein is the selection of a suitable expression host along with the right combination of a vector. Premas uses its proprietary software that was developed in-house to analyze the sequence of the protein of interest.

The training set consists of more than 900 protein sequences. Some of the parameters that are evaluated include the expression levels, hydrophobicity, solubility and ease of purification Till now we have had a >80% success rate in predicting the suitable host systems and the losses during purification. This initial feasibility study enables us to design the most appropriate strategy for protein production.


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Membrane Protein Expression

Price on request

"Premas has one of the largest facility for producing membrane preps/microsomal preps.”

To tackle the challenges faced by the industry in membrane protein production; Premas has developed yeast and mammalian expression systems with appropriate vectors.

We can produce more than 100 grams of membrane/ microsomal preps from a single batch.

Using a continuous ultracentrifuge we can obtain highly consistent batches of concentrated membrane preparations with a capacity of processing 100L of mammalian preparations in less than 2 hours.

Advantages of our system

  • High quality, active proteins
  • High yields of purified soluble proteins
  • Ability to produce hundreds of grams of protein
  • Reproducible, rapid and cost effective processes


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Recombinant Protein Expression

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DNA Services

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Toxicology

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Protein Services

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Biochemistry & Molecular Biology

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Protein Production

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Protein Sequencing

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Biology

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Nucleic Acid Services

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Pharmacology & Toxicology

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Data Services

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Computational Modeling

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Biomolecular Interaction Analysis

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Prabuddha Kundu

Co-Founder and MD

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