Premas Biotech

Premas performs and develops analytical techniques for in-process testing, end-product analysis and formulation / stability studies to deliver a high quality, active protein.

We possess the expertise and resources to purify from milligrams to grams of proteins and develop processes for purification of proteins from various sources. We have particularly developed a variety of protocols for isolation of insoluble proteins, on column refolding strategies, as well as membrane proteins. We deliver biologically active, pure and stable protein by choosing an efficient capture strategy and develop a robust purification process with minimal variation.

Our Services Include

• Protein purification using client-supplied protocols, standard protocols and customized protocols according to client's requirements.
  
• Protein purification method development for transfer to a GMP facility mg to gm to kg level scale up.
  
• Protein purification up to 99% purity.
  
• Optimization and development of purification method using different chromatographic techniques
  
  • Affinity
    
  • Ion Exchange
    
  • Size Exclusion
    
  • Reverse Phase
    
  • Hydrophobic Interaction
    
• Purification of recombinant tagged proteins (His6, GST, MBP, SUMO, FLAG, etc.) plus option of tag removal by proteolytic digestion (Thrombin, TEV, PreScission, SUMO protease, Factor Xa, etc.)
  
• Refolding and purification of insoluble proteins from inclusion bodies.
  
  • Solubilization and refolding studies.
    
• Optimizing storage conditions for various downstream applications.
  

• Design and synthesis of vectors based on your research needs or a vector of your choice.
  
• PCR cloning and subcloning services from customer provided templates.
  

One of the very first steps in the development of biopharmaceuticals or production of any recombinant protein is the selection of a suitable expression host along with the right combination of a vector. Premas uses its proprietary software that was developed in-house to analyze the sequence of the protein of interest.

The training set consists of more than 900 protein sequences. Some of the parameters that are evaluated include the expression levels, hydrophobicity, solubility and ease of purification Till now we have had a >80% success rate in predicting the suitable host systems and the losses during purification. This initial feasibility study enables us to design the most appropriate strategy for protein production.

"Premas has one of the largest facility for producing membrane preps/microsomal preps.”

To tackle the challenges faced by the industry in membrane protein production; Premas has developed yeast and mammalian expression systems with appropriate vectors.

We can produce more than 100 grams of membrane/ microsomal preps from a single batch.

Using a continuous ultracentrifuge we can obtain highly consistent batches of concentrated membrane preparations with a capacity of processing 100L of mammalian preparations in less than 2 hours.

Advantages of our system

• High quality, active proteins
  
• High yields of purified soluble proteins
  
• Ability to produce hundreds of grams of protein
  
• Reproducible, rapid and cost effective processes