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Premas Biotech

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Gurgaon, IN

About Premas Biotech

Founded: 2005 Type: Privately Held Size: 51-200 employees

Premas is a Contract Research and Manufacturing Services (CRAMS) organization located near New Delhi, India. It is formed on the strong foundation of scientific expertise and technology development which is dedicated to delivering solutions to our customers for their protein and critical reagent... Show more »

Premas is a Contract Research and Manufacturing Services (CRAMS) organization located near New Delhi, India. It is formed on the strong foundation of scientific expertise and technology development which is dedicated to delivering solutions to our customers for their protein and critical reagent requirements.

We have significant experience in handling "difficult -to-express" proteins and high-value-low-yield biomolecules. The company has both small scale as well as a cGMP compliant facility with a capacity for proteins, receptors and enzyme manufacturing of up to 1400 liters from E.coli and yeast cells and up to 100 liters from mammalian and baculovirus systems, along with continuous disk stack high speed and ultra centrifuges, high pressure homogenizers, purification and downstream equipments for large scale work.

This enables small scale rapid scouting for the correct expression and purification modalities, while the scale up group is trained to take this information and migrate to the large scale production facility.

To bring value to our customers , Premas has pioneered the implementation of Toyota Lean Management system coupled with E-Lab Notebook integrated with Scientific Database Management Systems (SDMS), which delivers accurate, rapid and 21CFR Part 11 compliant data that is of fundamental importance for regulatory purposes, data submission and conformance with external quality systems, The biggest benefit is the streamlining of externally sourced research to the internal systems of our customers, in terms of visibility, control, and delivery.

Our goal is to be your preferred partner for sourcing of your protein and critical reagent needs. We work hard to earn your business by upholding extremely high quality standards of research and maintaining aggressive timelines, strict confidentiality, and timely updates to deliver the product and value for the relationship.

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Our Services (21)


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Cell-Based Assays

Price on request
  • Proliferation
  • Apoptosis
  • Ex vivo cell viability
  • Cytotoxicity
  • Reporter gene (Luminescence)
  • Receptor- ligand binding & uptake (Fluorescence/ Luminescence)
  • Cytokine release (ELISA)
  • Proliferation
  • Apoptosis
  • Ex vivo cell viability
  • Cytotoxicity
  • Reporter gene (Luminescence)
  • Receptor- ligand binding & uptake (Fluorescence/ Luminescence)
  • Cytokine release (ELISA)
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Ligand Binding Assays

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Cytotoxicity Assays

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Protein Characterization

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Premas performs and develops analytical techniques for in-process testing, end-product analysis and formulation / stability studies to deliver a high quality, active protein.

S. No. Characterization Processes
1 Purity Analysis SDS-PAGE , HPLC , Mass-spectrometry
2 Quantity... Show more »

Premas performs and develops analytical techniques for in-process testing, end-product analysis and formulation / stability studies to deliver a high quality, active protein.

S. No. Characterization Processes
1 Purity Analysis SDS-PAGE , HPLC , Mass-spectrometry
2 Quantity Analysis Densitometry, Protein Estimation by Bradford method, Lowry and BCA method.
3 Identity Analysis Qualitative ELISA, Western Blot ,MALDI, Peptide Mapping
4 Contaminant Analysis Host Cell DNA, Host Cell Protein, Endotoxin Profile, HPLC
5 Aggregation Analysis DLS Method
6 Functionality Study Biochemical assays, Cell based Assays, SPR (Surface Plasmon Resonance)
7 Stability Analysis Lyophilization Study, Temperature Study, Buffer Compositions
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Protein Purification

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We possess the expertise and resources to purify from milligrams to grams of proteins and develop processes for purification of proteins from various sources. We have particularly developed a variety of protocols for isolation of insoluble proteins, on column refolding strategies, as well as membrane proteins. We deliver... Show more »

We possess the expertise and resources to purify from milligrams to grams of proteins and develop processes for purification of proteins from various sources. We have particularly developed a variety of protocols for isolation of insoluble proteins, on column refolding strategies, as well as membrane proteins. We deliver biologically active, pure and stable protein by choosing an efficient capture strategy and develop a robust purification process with minimal variation.

Our Services Include

  • Protein purification using client-supplied protocols, standard protocols and customized protocols according to client's requirements.
  • Protein purification method development for transfer to a GMP facility mg to gm to kg level scale up.
  • Protein purification up to 99% purity.
  • Optimization and development of purification method using different chromatographic techniques
    • Affinity
    • Ion Exchange
    • Size Exclusion
    • Reverse Phase
    • Hydrophobic Interaction
  • Purification of recombinant tagged proteins (His6, GST, MBP, SUMO, FLAG, etc.) plus option of tag removal by proteolytic digestion (Thrombin, TEV, PreScission, SUMO protease, Factor Xa, etc.)
  • Refolding and purification of insoluble proteins from inclusion bodies.
    • Solubilization and refolding studies.
  • Optimizing storage conditions for various downstream applications.
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Molecular Cloning

Price on request
  • Design and synthesis of vectors based on your research needs or a vector of your choice.
  • PCR cloning and subcloning services from customer provided templates.
  • Design and synthesis of vectors based on your research needs or a vector of your choice.
  • PCR cloning and subcloning services from customer provided templates.
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In Silico Protein Sequence Analysis

Price on request

One of the very first steps in the development of biopharmaceuticals or production of any recombinant protein is the selection of a suitable expression host along with the right combination of a vector. Premas uses its proprietary software that was developed in-house to analyze the sequence of the protein of interest.

The... Show more »

One of the very first steps in the development of biopharmaceuticals or production of any recombinant protein is the selection of a suitable expression host along with the right combination of a vector. Premas uses its proprietary software that was developed in-house to analyze the sequence of the protein of interest.

The training set consists of more than 900 protein sequences. Some of the parameters that are evaluated include the expression levels, hydrophobicity, solubility and ease of purification Till now we have had a >80% success rate in predicting the suitable host systems and the losses during purification. This initial feasibility study enables us to design the most appropriate strategy for protein production.

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Membrane Protein Expression

Price on request

"Premas has one of the largest facility for producing membrane preps/microsomal preps.”

To tackle the challenges faced by the industry in membrane protein production; Premas has developed yeast and mammalian expression systems with appropriate vectors.

We can produce more than 100 grams of membrane/ microsomal preps from a... Show more »

"Premas has one of the largest facility for producing membrane preps/microsomal preps.”

To tackle the challenges faced by the industry in membrane protein production; Premas has developed yeast and mammalian expression systems with appropriate vectors.

We can produce more than 100 grams of membrane/ microsomal preps from a single batch.

Using a continuous ultracentrifuge we can obtain highly consistent batches of concentrated membrane preparations with a capacity of processing 100L of mammalian preparations in less than 2 hours.

Advantages of our system

  • High quality, active proteins
  • High yields of purified soluble proteins
  • Ability to produce hundreds of grams of protein
  • Reproducible, rapid and cost effective processes
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Recombinant Protein Expression

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DNA Services

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Toxicology

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Protein Services

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Biochemistry & Molecular Biology

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Protein Production

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Protein Sequencing

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Biology

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Nucleic Acid Services

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Pharmacology & Toxicology

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Data Services

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Computational Modeling

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Biomolecular Interaction Analysis

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