Poochon Scientific

ID-101: Gel Bands/Gel Spots ID
Description: This service package is for identification of proteins (up to 500 proteins) from a gel band/spot.
Sample type: Gel band or spot (no bigger than 1.5×2×5 mm in size).
Services include: In-gel trypsin or chymotrypsin digestion, peptide extraction, 1 hour LC-MS/MS and result report.
Example workflow:
Protein ID from Gel Band
Protein digestion $20.00
LC-MS/MS (1hour Orbitrap) $200.00
Data analysis (half an hour) $30.00
TOTAL: $250.00
$200/sample for 5 or over

ID-102: Protein Mixture
Description: This service package is for identification of proteins (up to 2000 proteins) from any biological samples including cell lysates, tissue lysates, IP (immunoprecipitation) sample, pull-down sample, or proteins mixture.
Sample type: Frozen liquid, in-SDS sample buffer, or lyophilized.
Services include: Sample preparation for MS analysis, 2 hour LC-MS/MS analysis, and Result report.
Example Workflow:
Protein ID from Solution, Pull-Down or co-IP
Protein digestion $20.00
LC-MS/MS (2 hours Orbitrap) $400.00
Data analysis (half an hour) $30.00
TOTAL: $450.00
$400/sample for 5 or over

Turnaround time: 7-10 business days

High-Resolution Characterization of Protein Therapeutics ( Antibody )

HC-101: Purity Evaluation $600
Description: This service package is for evaluation of the purity of purified protein samples using LC/MS/MS. The percentages of trace proteins from the host if identified will be determined based on label-free quantitation method.
Sample type: purified protein samples in liquid or dried form (>= 5 µg/sample, purity >80%).
Services include: Sample preparation, LC-MS/MS analysis, data analysis and result report.
Turnaround time: 7-10 business days or less

HC-102: Full Amino Acid Sequence Confirmation $800
Description: This service package is for confirmation of full amino acid sequence (>95% sequence coverage) of any purified protein sample (target protein amino acid sequence known) using LC/MS/MS.
Sample type: purified protein samples in liquid or dried form (>= 5 µg/sample, purity >80%).
Services include: Sample preparation, LC-MS/MS analysis, data analysis and result report.
Turnaround time: 10-14 business days or less

HC-103: Identification of N-linked Glycosylation $1,800
Description: This service package is for identification of N-linked glycosylation site from purified protein monoclonal antibody samples (including monoclonal antibody and any other purified protein) using PNGF digestion and LC/MS/MS. The percentages of glycosylation will be determined using label-free quantitation method.
Sample type: purified protein samples in liquid or dried form (>= 20 µg/sample, purity >99%).
Services include: Sample preparation, LC-MS/MS analysis, data analysis and result report.
Turnaround time: 10-14 business days or less

HC-104: Profile of N-linked Glycans for mAb $5,000
Description: This service package is for evaluation of the glycan profile of purified monoclonal antibody samples using LC/MS/MS. The percentages of trace proteins from the host if identified will be determined based on label-free quantitation method.
Sample type: purified protein samples in liquid or dried form (>= 20 µg/sample, purity >99%).
Services include: Sample preparation, LC-MS/MS analysis, data analysis and result report.
Turnaround time: 14-20 business days or less

Proteomic Profiling and Quantification

QT-101: Label-free Quantitative Proteome Profiling $2,800/sample
Description: This service package is for proteome profiling (identify up to 5,000 proteins per sample) from human/mouse tissue or cell line samples using label-free quantitation method.
Sample type: Frozen cell pellet (>= 2×106 cell/sample), Fresh frozen tissues (>= 200 mg/sample).
Services include: Protein lysate preparation, fractionation (16 fractions), digestion, 16 LC-MS/MS 110 min runs, common pathway analysis, and result report.
Turnaround time: 14-20 business days or less

QT-102: Label-free Quantitative Proteome Profiling $3,800
Description: This service package is for proteome profiling (identify up to 7,000 proteins per sample) from human/mouse tissue or cell line samples using label-free quantitation method.
Sample type: Frozen cell pellet (>= 2×106 cell/sample), Fresh frozen tissues (>= 200 mg/sample).
Services include: Protein lysate preparation, fractionation (24 fractions), digestion, 24 LC-MS/MS 110 min runs, common pathway analysis, and result report.
Turnaround time: 14-20 business days or less

QT-201: TMTsixplex-Labeling Quantitative Proteome Profiling $8,000-$10,000/2-6 samples
Description: This service package is for quantitatively comparison of the proteomes (>4000 proteins) of 2-6 samples in one set of analysis using Thermo TMTsixplex™ Isobaric Label Reagent.
Sample type: Frozen cell pellet (>= 1×107 cell/sample), Frozen tissue (>=500 mg/sample).
Services include: Protein lysate preparation, TMT-labeling, UHPLC fractionation, digestion, LC-MS/MS analysis, TMT quantitation, Pathway analysis, and result report.
Turnaround time: 14-20 business days

QT-202: TMT10plex-Labeling Quantitative Proteome Profiling $12,000-$15,000/7-10 samples
Description: This service package is for quantitatively comparison of the proteomes (>4000 proteins) of 7-10 samples in one set of analysis using Thermo TMT10plex™ Isobaric Label Reagent. Sample type: Frozen cell pellet (>= 1×107 cell/sample), Frozen tissue (>=500 mg/sample).
Services include: Protein lysate preparation, TMT-labeling, UHPLC fractionation, digestion, LC-MS/MS analysis, TMT quantitation, Pathway analysis, and result report.
Turnaround time: 14-20 business days

PTM-101: PTM Identification $600/sample
Description: This service package is for identification of PTMs including S/T/Y phosphorylation, R/K methylation, K/H acetylation, K ubiquitination, etc. from crude purified protein samples.
Sample type: crude purified protein sample such as IP or pull-down sample, or gel band sample contains more than 5 µg of protein.
Services include: Sample preparation, LC-MS/MS analysis, Data analysis and result report.
Turnaround time: 10-14 business days or less

PTM-102: Phosphoprotein profiling $2,000/sample
Description: This service package uses label-free quantitation method and phosphopeptides enrichment kit for profiling of phosphoproteins among different samples.
Sample type: Fresh frozen cell pellets (>= 2×107 cell/sample), fresh frozen tissues (>=500 mg/sample), or fresh frozen total cell or tissue lysates (200 µl at 5 mg/ml).
Services include: Sample preparation, Phosphopeptides enrichment, LC-MS/MS analysis, data analysis and result report.
Turnaround time: 14-20 business days or less

PTM-103: TMTsixplex-labeling Quantitative Phosphoprotein profiling $9,000-$12,000/2-6 samples
Description: This service package quantitatively compare the Phospho-proteomes of 2-6 samples in one set of analysis using Thermo TMTsixplex™ Isobaric Label Reagent and Phosphopeptides enrichment kit.
Sample type: Fresh frozen cell pellets (>= 2×107 cell/sample), fresh frozen tissues (>=500 mg/sample), or fresh frozen total cell or tissue lysates (200 µl at 5 mg/ml per sample).
Services include: Sample preparation, TMT-labeling, Phosphopeptides enrichment, LC-MS/MS analysis, data analysis and result report.
Turnaround time: 14-20 business days or less

PTM-104: TMT10plex-labeling Quantitative Phosphoprotein profiling $12,000-$16,000/7-10 samples
Description: This service package quantitatively compare the Phospho-proteomes of 7-10 samples in one set of analysis using Thermo TMT10plex™ Isobaric Label Reagent and Phosphopeptides enrichment kit.
Sample type: Fresh frozen cell pellets (>= 2×107 cell/sample), fresh frozen tissues (>=500 mg/sample), or fresh frozen total cell or tissue lysates (200 µl at 5 mg/ml per sample).
Services include: Sample preparation, TMT-labeling, Phosphopeptides enrichment, LC-MS/MS analysis, data analysis and result report.
Turnaround time: 14-20 business days or less

Poochon developed a novel pathway analysis tool called PROMICPATH. The PROMICPATH provides both 42 common pathways analysis services (available for human and mouse samples) and customized pathway analysis services. To ensure the integrity and reliability the PROMICPATH uses 10 groups of 400 housekeeping proteins to evaluate the completeness and variability of a proteome profile. An example of output of PROMICPATH analysis is illustrated below.

PA-101: Pathway Analysis $1,000/set of data analysis
Description: This service package is for common cellular and signaling pathways analysis as well as evaluation of the completeness and variability of a proteome profile.
Sample type: MS raw files.
Services include: Common cellular and signaling pathway analysis.
Turnaround time: 7-10 business days

BD-101: Biomarker Screening
Description: This service package is for identification of differentially expressed proteins from two groups of samples such as health and disease. The client chose one of our Quantitative Proteome profiling service packages: QT-102, QT-201, or QT-202.
Sample type: Frozen cell pellet (>= 1×107 cell/sample), Frozen tissue (>=500 mg/sample).
Services include: QT-102, QT201, or QT-202.
Turnaround time: 20-30 business days

CUS-101: Client Designed Project
Description: This flexible custom service allows the client design the project workflow and requirements. Poochon prepare a proposal with cost estimation based on the client designed workflow and requirements.
Sample type: any biological samples.
Services include: Sample preparation, LC-MS/MS analysis, data analysis and result report.
Turnaround time: based on project

CUS-102: Poochon Designed Project
Description: This flexible custom service allows the client specifies the project goals and specifications. Poochon prepare a proposal with cost estimation based on the client specified goals and specifications.
Sample type: any biological samples.
Services include: Sample preparation, LC-MS/MS analysis, data analysis and result report.
Turnaround time: based on project

PR-101: Cell lysate
Description: This service is for making protein lysate from cells.
Sample type: Fresh frozen cell pellet. (For profiling >1x106 cells/sample)
Services include: lyse cell, homogenization, sonication, protein content measurement using BCA kit.
Price: $100/pair of samples

PR-102: Tissue lysate
Description: This service is for making protein lysate from tissues.
Sample type: Fresh frozen tissues. (For profiling > 100mg/sample)
Services include: lyse tissue, homogenization, sonication, protein content measurement using BCA kit.
Price: $125/pair of samples

PR-103: SDS-PAGE
Description: This service is for separation of proteins on a 4-12% NuPAGE gel.
Sample type: Frozen liquid, in-SDS sample buffer, non-covalent on-matrix or lyophilized.
Services include: SDS-PAGE, SimplyBlue Stain, and Imaging.
Price: $125/1-9 samples

PR-104: In-gel digestion
Description: This service is for trypsin or chymotrypsin digestion of proteins from gel band/spot samples
Sample type: Gel band or spot. Each sample should contain only one gel piece, no bigger than 1.5x2x5mm in size.
Services include: In-gel trypsin/chymotrypsin digestion, peptide extraction, C18 zip-tip cleanup.
Price: $100/sample

PR-105: In-solution digestion
Description: This service is for FASP (Filter Aided Sample Preparation) in-solution digest of protein mixture.
Sample type: Frozen liquid, non-covalent on-matrix or lyophilized.
Services include: In-solution digestion, peptide extraction, C18 zip-tip cleanup.
Price: $150/sample

PR-106: TMT labeling
Description: This service is for preparing TMT-labeled peptides for quantitative proteome profiling. The labeling uses Thermo TMTsixplex™ or 10plex Isobaric Label Reagent and the fractionation is carried out by basic reverse phase UPLC.
Sample type: Frozen lysate, frozen cell pellet, or frozen tissue.
Services include: Protein lysate, trypsin digestion, TMT-labeling, UPLC Fractionation.
Price: $5000-$8000/set

PR-107: LC-MS/MS – Standard 60 min LC gradient run
Description: This service is for analysis of peptide mixture by LC/MS/MS using QE and Ultimate 3000 nano RSLC system for a standard 60 min LC gradient run.
Sample type: Digests from in-gel or in-solution (<= 5 µg/sample)
Services include: C18 zip-tip cleanup, 60 min LC-MS/MS run, Database search and Protein ID report.
Price: $150/sample

PR-107: LC-MS/MS – Standard 120 min LC gradient run.
Description: This service is for analysis of peptide mixture by LC/MS/MS using QE and Ultimate 3000 nano RSLC system for a standard 6120 min LC gradient run.
Sample type: Digests from in-gel or in-solution (<=5 µg/sample)
Services include: C18 zip-tip cleanup, 120 min LC-MS/MS run, Database search and Protein ID report.
Price: $250/sample

PR-108: PTM Analysis Report
Description: Summarize identified PTM sites for all samples in one table using Poochon PTMsites software. Excel table list all residual positions in corresponding to S/T/Y phosphorylation, R/K methylation, K acetylation, K ubiquitination, etc. Label-free Quantification using Peptide spectrum match counts (PSM) based or peak area based.
Data type: MS raw data files of PD msf files
Price: $200/set

PR-109: Pathway analysis
Description: Using Poochon PROMICPATH to evaluate the integrity of a proteome profile and perform functional related pathway analysis including common and customized pathways.
Data type: MS raw data files.
Result format: Excel tables and heat maps.
Price: $1000/pair of datasets

Top of the line instrumentation at Poochon Scientific:
Thermo Fisher Q Exactive Hybrid Quadrupole-Orbitrap Mass Spectrometer
UltiMate 3000 RSLCnano System
Agilent 1290 Infinity UHPLC System

Poochon Services: Accrate, Informative, Fast, Afordable