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PNA BIO INC

Thousand Oaks, California, US

PNA BIO INC is devoted to provide quality research products and services to the laboratories of basic research, diagnostics, drug discovery, and applied field.

PNA Bio offer quality PNA (Peptide Nucleic Acid) products including custom oligos, FISH probes, miRNA inhibitors, PNA clamp probes and Fmoc monomers. They are provided >95% purity after HPLC purification along with MS/MS data.

PNA Bio also provides CRISPR/Cas9 nuclease services, which includes custom sgRNA synthesis and validation service, KO/KI cell line service, Cas9 protein, and Cas9 mRNA.

PNA BIO INC has established a sterling reputation built on innovative products and excellent customer support. In addition to providing a quality products and paying attention to details, we also thrive on strong technical support that is freely accessible in a timely manner.

PNA BIO INC has not listed any services.

Cas9 Nuclease
Starting at $75.00 per unit

Cas9 protein with NLS, injection and transfection ready
CP01-20 (20 ug size, reconstitutes to 1 mg/ml) - $75
CP01-50 (50 ug size, reconstitutes to 1 mg/ml) - $115
CP01-200 (50 ug x 4 tubes, reconstitutes to 1 mg/ml) - $350
CP02-250 (250 ug size, reconstitutes to 5 mg/ml) - $400

Fluorescent Cy3 labeled Cas9 protein that is formulated at 1 mg/ml or 5 mg/ml also offered.

Cas9 protein with NLS, injection and transfection ready
CP01-20 (20 ug size, reconstitutes to 1 mg/ml) - $75
CP01-50 (50 ug size, reconstitutes to 1 mg/ml) - $115
CP01-200 (50 ug x 4 tubes, reconstitutes to 1 mg/ml) - $350
CP02-250 (250 ug size, reconstitutes to 5 mg/ml) - $400

Fluorescent Cy3 labeled Cas9 protein that is formulated at 1 mg/ml or 5 mg/ml also offered.

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sgRNA Synthesis
Price on request

Custom sgRNA IVT Synthesis, ready for injection or transfection.
We offer 50 ug of custom sgRNA (guaranteed amount) with gel picture.

Custom sgRNA IVT Synthesis, ready for injection or transfection.
We offer 50 ug of custom sgRNA (guaranteed amount) with gel picture.

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CRISPR/Cas9 Vector Construction
Starting at $350.00 per unit

We offer custom sgRNA synthesis service for DNA or RNA format. It includes design, off-target search, and synthesis of custom vector.

We also offer various Cas9 vectors, under CMV or EF1a promoter, multiple cassette with RFP-PuroR or GFP-HygroR. Nickase vectors are also available.

We offer custom sgRNA synthesis service for DNA or RNA format. It includes design, off-target search, and synthesis of custom vector.

We also offer various Cas9 vectors, under CMV or EF1a promoter, multiple cassette with RFP-PuroR or GFP-HygroR. Nickase vectors are also available.

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gRNA Validation
Price on request

We offer guide RNA validation service in cells. For this, we construct4 gRNA vectors and transfect them to mouse or human cells to test the activity. We select the most active gRNA and make it into sgRNA, injection ready form.
We will send the sgRNA vector, sgRNA IVT, and PCR primers to the researcher along with the complete... Show more »

We offer guide RNA validation service in cells. For this, we construct4 gRNA vectors and transfect them to mouse or human cells to test the activity. We select the most active gRNA and make it into sgRNA, injection ready form.
We will send the sgRNA vector, sgRNA IVT, and PCR primers to the researcher along with the complete protocol for T7E1 assay (mismatch sensitive nuclease assay) that can be used for screening.

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Stable Cell Line Generation
Price on request

We provide custom KO and KI cell line service. With years of experience of ZFN, TALEN, and CRISPR/Cas9 technology, you can rely on us to deliver the most affordable, fast, and reliable engineered cell lines in 4~7 months. We provide at least two clones that are confirmed to be mycoplasma free for each KO project.

We provide custom KO and KI cell line service. With years of experience of ZFN, TALEN, and CRISPR/Cas9 technology, you can rely on us to deliver the most affordable, fast, and reliable engineered cell lines in 4~7 months. We provide at least two clones that are confirmed to be mycoplasma free for each KO project.

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PNA Synthesis
Peptide Nucleic Acid Synthesis
Price on request

Custom PNA oligos can be unlabeled or labeled by dyes or other chemicals. They can also be conjugated to peptides to add other functionality. Linkers can be added as a spacer and also to improve solubility. Gamma PNA or modified bases can increase Tm and specificity of PNA even further.

About PNA

PNA (Peptide Nucleic... Show more »

Custom PNA oligos can be unlabeled or labeled by dyes or other chemicals. They can also be conjugated to peptides to add other functionality. Linkers can be added as a spacer and also to improve solubility. Gamma PNA or modified bases can increase Tm and specificity of PNA even further.

About PNA

PNA (Peptide Nucleic Acid) is an artificially synthesized polymer similar to DNA or RNA. The various purine and pyrimidine bases are linked to the backbone by methylene carbonyl bonds as in peptides. Since PNA contains no charged phosphate groups, the binding between PNA and DNA is stronger than that between DNA and DNA due to the lack of electrostatic repulsion. PNA is resistant to DNases and proteases, and is extremely stable in vivo as well as in vitro.

PNA Applications

  • Sequence specific PCR blocker (PNA clamp)
  • FISH probes for telomere, centromere, gene specific probes, infection test
  • Anti-sense/ anti-microbial reagents
  • miRNA inhibitors
  • Double strand DNA invasion & capture
  • Microarray probes
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PNA Probes
Starting at $240.00 per unit

PNA FISH probes (telomere, centromere, or custom)

We offer PNA probes specific for telomere, centromere, and CAG repeats that are labeled with various fluorophores and biotin. PNA probes have superior affinity and selectivity for the target sequence over DNA probes, so hybridization is fast and sequence-specific.

PNA FISH probes (telomere, centromere, or custom)

We offer PNA probes specific for telomere, centromere, and CAG repeats that are labeled with various fluorophores and biotin. PNA probes have superior affinity and selectivity for the target sequence over DNA probes, so hybridization is fast and sequence-specific.

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SNP Detection
Starting at $400.00 per unit

Due to its high affinity and specificity, PNA oligos can efficiently bind its target nucleic acid. One of the popular usage of unlabeled PNA is a gene specific blocker of PCR reaction (PNA clamp). This technology can efficiently detect SNP mutations in a target gene.

Simply by mixing PNA oligo for WT DNA in PCR reaction, DNA... Show more »

Due to its high affinity and specificity, PNA oligos can efficiently bind its target nucleic acid. One of the popular usage of unlabeled PNA is a gene specific blocker of PCR reaction (PNA clamp). This technology can efficiently detect SNP mutations in a target gene.

Simply by mixing PNA oligo for WT DNA in PCR reaction, DNA with SNP mutation is specifically amplified in PCR reaction, which allows genotyping efficient and easier.

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PNA Clamp
PNA Oligos
Peptide Nucleic Acid Oligomers
Starting at $335.00 per unit

PNA clamp can be an efficient PCR blocker to reduce the background in sequencing reaction. By simply adding PNA oligo that matches to the target sequence in your PCR reaction, you can reduce the background. mPNA and pPNA has been successfully used to suppress background generated by mitochondria and plastid 16s ribosomal RNA, which enriches target specific amplification.

PNA clamp can be an efficient PCR blocker to reduce the background in sequencing reaction. By simply adding PNA oligo that matches to the target sequence in your PCR reaction, you can reduce the background. mPNA and pPNA has been successfully used to suppress background generated by mitochondria and plastid 16s ribosomal RNA, which enriches target specific amplification.

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PNA miRNA Inhibitors
Starting at $250.00 per unit

Since PNA binds complementary RNA more strongly than DNA or RNA does, it offers superior activity in relative to RNA/DNA based miRNA inhibitors. ln addition, PNA miRNA (microRNA) inhibitors are conjugated to cell penetrating peptide (CPP), allowing them enter to cells without the need of transfection reagents.
The use of PNA... Show more »

Since PNA binds complementary RNA more strongly than DNA or RNA does, it offers superior activity in relative to RNA/DNA based miRNA inhibitors. ln addition, PNA miRNA (microRNA) inhibitors are conjugated to cell penetrating peptide (CPP), allowing them enter to cells without the need of transfection reagents.
The use of PNA miRNA inhibitors has been shown to be a valuable tool in vivo.

Key Features
PNAs™ miRNA inhibitors are highly sequence-specific
PNAs™ miRNA inhibitors can be used without using transfection reagent
PNAs™ miRNA inhibitors have long half life in cells, up to 14 days
PNAs™ miRNA inhibitors are resistant and very stable cellular nucleases
PNAs™ miRNA inhibitors have no cytotoxicity

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Biology
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Cells and Tissues
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Cell and Tissue Culture
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Genetic Engineering
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Biochemistry & Molecular Biology
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Nucleic Acid Services
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DNA Services
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Genotyping Services
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Plasmids and Vectors
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RNA Services
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PNA Services
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2018-09-08 15:40:40 -1000

Net Promoter Score of 10 received for Cas9 Nuclease.

Additional Ratings: satisfaction with deliverable: 10, satisfaction with timeliness: 10.
2018-03-29 06:24:35 -1000

Net Promoter Score of 10 received for Cas9 Nuclease.

Additional Ratings: satisfaction with deliverable: 9, satisfaction with timeliness: 8.
2017-07-05 12:24:16 -1000

Net Promoter Score of 10 received for PNA Synthesis.

Additional Ratings: satisfaction with deliverable: 10, satisfaction with timeliness: 9.
2017-07-05 12:23:35 -1000

Net Promoter Score of 10 received for PNA Synthesis.

Additional Ratings: satisfaction with deliverable: 10, satisfaction with timeliness: 9.
2017-07-05 12:22:55 -1000

Net Promoter Score of 10 received for PNA Synthesis.

Additional Ratings: satisfaction with deliverable: 10, satisfaction with timeliness: 9.
2017-06-27 06:27:51 -1000

Net Promoter Score of 8 received for sgRNA Synthesis.

Additional Ratings: satisfaction with deliverable: 8, satisfaction with timeliness: 8.
2017-06-26 10:40:42 -1000

Net Promoter Score of 10 received for PNA Synthesis.

Additional Ratings: satisfaction with deliverable: 10, satisfaction with timeliness: 10.
2017-05-15 10:47:12 -1000

Net Promoter Score of 10 received for gRNA Validation.

Additional Ratings: satisfaction with deliverable: 10, satisfaction with timeliness: 10.
2017-05-15 10:15:39 -1000

Net Promoter Score of 10 received for PNA Synthesis.

Additional Ratings: satisfaction with deliverable: 10, satisfaction with timeliness: 10.
2017-05-15 10:15:39 -1000

Net Promoter Score of 10 received for PNA Synthesis.

Additional Ratings: satisfaction with deliverable: 10, satisfaction with timeliness: 10.
February 27, 2017

Positive review received for PNA Synthesis:

"Have had several successful orders through PNA BIO. Very friendly and communicative"

February 6, 2017

Positive review received for PNA Synthesis:

"Helen is a pleasure to work with and PNA bio continues to provide great custom PNA synthesis"

December 1, 2016

Positive review received for PNA Synthesis:

"Very friendly and professional and quick turnaround!"

2016-08-04 08:38:26 -1000

Net Promoter Score of 10 received for CRISPR Modified Cell Lines.

Additional Ratings: satisfaction with deliverable: 9, satisfaction with timeliness: 8.

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