Tdjtyq9qeclgatu6dfba osu

Plant-Microbe Genomics Facility

Columbus, Ohio, US

The Plant-Microbe and Genomics Facility provides services to the researchers at Ohio State University and beyond for the study of DNA to proteins from any organism.

The Facility, established in 2000, serves the entire Ohio State University as well as the researchers in the state of Ohio and beyond, by providing resources in order to study genomes from DNA sequence to protein activities. Despite our name, the Facility can study any organism's DNA, RNA or protein. The Facility provides 5 major services:

  1. DNA Sequencing
  2. Genotyping
  3. Gene Promoter Characterization
  4. Real-time PCR
  5. Biomolecular Interaction Analysis.

The procedures are designed to provide the opportunity to obtain high quality results at a reasonable cost.

Recent Publications

  • Wrighton KC, Castelle CJ, Varaljay VA, Satagopan S, Brown CT, Wilkins MJ, Thomas BC, Sharon I, Williams KH, Tabita FR, Banfield JF. RubisCO of... Show more »

The Plant-Microbe and Genomics Facility provides services to the researchers at Ohio State University and beyond for the study of DNA to proteins from any organism.

The Facility, established in 2000, serves the entire Ohio State University as well as the researchers in the state of Ohio and beyond, by providing resources in order to study genomes from DNA sequence to protein activities. Despite our name, the Facility can study any organism's DNA, RNA or protein. The Facility provides 5 major services:

  1. DNA Sequencing
  2. Genotyping
  3. Gene Promoter Characterization
  4. Real-time PCR
  5. Biomolecular Interaction Analysis.

The procedures are designed to provide the opportunity to obtain high quality results at a reasonable cost.

Recent Publications

  • Wrighton KC, Castelle CJ, Varaljay VA, Satagopan S, Brown CT, Wilkins MJ, Thomas BC, Sharon I, Williams KH, Tabita FR, Banfield JF. RubisCO of a nucleoside pathway known from Archaea is found in diverse uncultivated phyla in bacteria. ISME J. 2016 May 3. doi: 10.1038/ismej.2016.53. [Epub ahead of print]
  • Varaljay VA, Satagopan S, North JA, Witte B, Dourado MN, Anantharaman K, Arbing MA, McCann SH, Oremland RS, Banfield JF, Wrighton KC, Tabita FR. Functional metagenomic selection of ribulose 1, 5-bisphosphate carboxylase/oxygenase from uncultivated bacteria. Environ Microbiol. 2016 Apr;18(4):1187-99. doi: 10.1111/1462-2920.13138.
  • Dey S, North JA, Sriram J, Evans BS, Tabita FR. In Vivo Studies in Rhodospirillum rubrum Indicate That Ribulose-1,5-bisphosphate Carboxylase/Oxygenase (Rubisco) Catalyzes Two Obligatorily Required and Physiologically Significant Reactions for Distinct Carbon and Sulfur Metabolic Pathways. J Biol Chem. 2015 Dec 25;290(52):30658-68. doi: 10.1074/jbc.M115.691295.
  • Kreel NE, Tabita FR. Serine 363 of a Hydrophobic Region of Archaeal Ribulose 1,5-Bisphosphate Carboxylase/Oxygenase from Archaeoglobus fulgidus and Thermococcus kodakaraensis Affects CO2/O2 Substrate Specificity and Oxygen Sensitivity. PLoS One. 2015 Sep 18;10(9):e0138351. doi: 10.1371/journal.pone.0138351.
  • Dangel AW, Tabita FR. CbbR, the Master Regulator for Microbial Carbon Dioxide Fixation. J Bacteriol. 2015 Nov;197(22):3488-98. doi: 10.1128/JB.00442-15.
  • Farmer RM, Tabita FR. Phosphoribulokinase mediates nitrogenase-induced carbon dioxide fixation gene repression in Rhodobacter sphaeroides. Microbiology. 2015 Nov;161(11):2184-91. doi: 10.1099/mic.0.000160.
« Show less

Plant-Microbe Genomics Facility has not listed any services.

Ask A Question Find what you're looking for? If not, you can ask this lab a question:
Robotics
Price on request

The Facility provides a robotic liquid handling service through the Beckman Biomek FX instrument. In addition to its role as a complex liquid handler, the Biomek FX is also a biochemistry workstation. This robot has a 96-tip head for liquid handling and a set of gripper arms to move objects around its 20 position deck. Essentially... Show more »

The Facility provides a robotic liquid handling service through the Beckman Biomek FX instrument. In addition to its role as a complex liquid handler, the Biomek FX is also a biochemistry workstation. This robot has a 96-tip head for liquid handling and a set of gripper arms to move objects around its 20 position deck. Essentially it can do anything you can do with a pipette, but 96-tips at a time.

« Show less
Genotyping Services
Price on request

The Facility can perform Genotyping by fragment analysis. The Facility has a 3730 DNA Analyzer (Applied Biosystems, Inc.) that can analyze DNA fragments labeled with a fluorescent dye to determine the size of the fragment with a resolution of one base. With 48 capillaries the instrument can analyze up to 48 samples simultaneously... Show more »

The Facility can perform Genotyping by fragment analysis. The Facility has a 3730 DNA Analyzer (Applied Biosystems, Inc.) that can analyze DNA fragments labeled with a fluorescent dye to determine the size of the fragment with a resolution of one base. With 48 capillaries the instrument can analyze up to 48 samples simultaneously as well as accommodate 96-, or 384-well plates. The 3730 DNA Analyzer has the ability to multiplex within one capillary by the use of 5 different fluorescent dyes, and/or differing fragment size.

The Facility can perform such techniques as allelic determination, amplified length polymorphism analysis (AFLP), microsatellite analysis (STR and SSR), and single nucleotide polymorphism analysis (SNapshot).

« Show less
Applied Biosystems 3730xl DNA Sequencer
RT-PCR
Reverse transcription polymerase chain reaction
Price on request

The facility has a CFX96 Real-Time Detection System (BioRad) which can accurately quantitate PCR products in real time. The CFX96 system is very sensitive and can simultaneously measure up to 6 different PCR products in each of 96-wells. This system is adept at precisely measuring the concentration of specific RNAs, measuring gene... Show more »

The facility has a CFX96 Real-Time Detection System (BioRad) which can accurately quantitate PCR products in real time. The CFX96 system is very sensitive and can simultaneously measure up to 6 different PCR products in each of 96-wells. This system is adept at precisely measuring the concentration of specific RNAs, measuring gene copy number as well as perfoming other applicatons that require the measurement of specific DNA molecules. The CFX96 system can analyze all of the current detection chemistries, such as SYBR Green, Taqman, etc. In addition, the instrument can be used as a specialized fluorometric based plate reader that can detect most dyes for applications such as high resolution melt analysis, single nucleotide polymorphism detection, and measuring protein melting.

The service includes the use of the CFX96 system and a file with the results. In addition, the Facility can provide a copy of the CFX96 software at no cost to you. The Facility will provide the 96-well plate and optical film which is included in the per run cost. The customer will be responsible for providing all other consumables (e.g. primers, polymerase, nucleotides, etc.) and setting up the reactions in the plate.

« Show less
DNA Sequencing
Price on request

Sanger Capillary
The facility uses the 3730 DNA Analyzer from Applied Biosystems, Inc. and BigDye® Terminator Cycle Sequencing chemistry. This automated instrument utilizes fluorescent dye labeled nucleotides and provides high volume capabilities, e.g. 384 sequencing reactions in 12 hours, and typically yields 800 to 1000... Show more »

Sanger Capillary
The facility uses the 3730 DNA Analyzer from Applied Biosystems, Inc. and BigDye® Terminator Cycle Sequencing chemistry. This automated instrument utilizes fluorescent dye labeled nucleotides and provides high volume capabilities, e.g. 384 sequencing reactions in 12 hours, and typically yields 800 to 1000 bases per reaction depending upon the quality and nature of the template

« Show less
Applied Biosystems 3730xl DNA Sequencer
DNA Fragment Analysis
Price on request

The Facility has a 3730 DNA Analyzer (Applied Biosystems, Inc.) that can analyze DNA fragments labeled with a fluorescent dye to determine the size of the fragment with a resolution of one base. With 48 capillaries the instrument can analyze up to 48 samples simultaneously as well as accommodate 96-, or 384-well plates. The 3730... Show more »

The Facility has a 3730 DNA Analyzer (Applied Biosystems, Inc.) that can analyze DNA fragments labeled with a fluorescent dye to determine the size of the fragment with a resolution of one base. With 48 capillaries the instrument can analyze up to 48 samples simultaneously as well as accommodate 96-, or 384-well plates. The 3730 DNA Analyzer has the ability to multiplex within one capillary by the use of 5 different fluorescent dyes, and/or differing fragment size.

The Facility can perform such techniques as allelic determination, amplified length polymorphism analysis (AFLP), microsatellite analysis (STR and SSR), and single nucleotide polymorphism analysis (SNapshot).

« Show less
High Resolution Melting Genotyping
Price on request
Request a quote for more information about this service.
Sanger Sequencing
Price on request

The facility uses the 3730 DNA Analyzer from Applied Biosystems, Inc. and BigDye® Terminator Cycle Sequencing chemistry. This automated instrument utilizes fluorescent dye labeled nucleotides and provides high volume capabilities, e.g. 384 sequencing reactions in 12 hours, and typically yields 800 to 1000 bases per reaction depending upon the quality and nature of the template.

The facility uses the 3730 DNA Analyzer from Applied Biosystems, Inc. and BigDye® Terminator Cycle Sequencing chemistry. This automated instrument utilizes fluorescent dye labeled nucleotides and provides high volume capabilities, e.g. 384 sequencing reactions in 12 hours, and typically yields 800 to 1000 bases per reaction depending upon the quality and nature of the template.

« Show less
Surface Plasmon Resonance (SPR)
Price on request

The Facility has a GE Healthcare Biacore T100 that can measure the interaction between proteins and proteins, DNA and proteins, metabolites and proteins, as well as other biomolecules that can be anchored to a gold covered chip.The measurements are done in real-time with no labels and utilize surface plasmon resonance (SPR)... Show more »

The Facility has a GE Healthcare Biacore T100 that can measure the interaction between proteins and proteins, DNA and proteins, metabolites and proteins, as well as other biomolecules that can be anchored to a gold covered chip.The measurements are done in real-time with no labels and utilize surface plasmon resonance (SPR) technology. The T100 can measure concentration, binding specificity, and affinity constants.

Due to the nature of the studies performed with the T100, the client uses the instrument in order to allow for instant analysis of the results and therefore immediate decisions about the next step or experiment to be done. In order to use the instrument, all clients are required to attend a training session provided by the Facility or the Biacore Basics course offered by GE Healthcare. The training provided by the staff is done at the Facility, takes one to three days, and can accommodate up to 2 clients at a time. The client will need to provide all materials and training will utilize the client's samples. An experienced staff member will provide guidance during the initial experiment and the training has a set fee. The training will include: practical use of the instrument, experimental design, and analysis.

« Show less
Biacore
DNase Footprinting Assay
Price on request
Request a quote for more information about this service.
EMSA
Electrophoretic Mobility Shift Assay
Price on request
Request a quote for more information about this service.
RNA SHAPE Analysis
Selective 2’-Hydroxyl Acylation analyzed by Primer Extension
Price on request

The Facility is pleased to announce a new service - Selective 2’-Hydroxyl Acylation analyzed by Primer Extension (SHAPE). SHAPE is a method of RNA analysis that is used to determine RNA structure at the nucleotide level. RNA molecules are combined with a hydrolyzing agent, such as benzoyl cyanide (BzCN), which selectively... Show more »

The Facility is pleased to announce a new service - Selective 2’-Hydroxyl Acylation analyzed by Primer Extension (SHAPE). SHAPE is a method of RNA analysis that is used to determine RNA structure at the nucleotide level. RNA molecules are combined with a hydrolyzing agent, such as benzoyl cyanide (BzCN), which selectively hydrolyzes unpaired nucleotides, forming 2’-O-adducts. RNA molecules then undergo a primer extension reaction, which the adducts inhibit, forming cDNA fragments that terminate at the location of hydrolysis. These fragments are analyzed on the 3730 DNA Analyzer (Applied Biosystems, Inc.) along with a negative control and one or two dideoxy sequencing reactions. The electropherograms from these reactions are aligned with each other, as well as a reference sequence, to determine the identity of unpaired nucleotides in the original RNA molecule.

« Show less
Electrophoresis
Price on request
Request a quote for more information about this service.
Engineering
Price on request
Request a quote for more information about this service.
PCR
Price on request
Request a quote for more information about this service.
Purification Services
Price on request
Request a quote for more information about this service.
Nucleic Acid Services
Price on request
Request a quote for more information about this service.
Protein-DNA Interaction Analysis
Price on request
Request a quote for more information about this service.
DNA
Price on request
Request a quote for more information about this service.
Biomolecular Interaction Analysis
Price on request
Request a quote for more information about this service.
Gel Electrophoresis
Price on request
Request a quote for more information about this service.
Chemistry and Materials
Price on request
Request a quote for more information about this service.
RNA
Price on request
Request a quote for more information about this service.
Biochemistry & Molecular Biology
Price on request
Request a quote for more information about this service.
Devices
Price on request
Request a quote for more information about this service.
Analytical Chemistry Services
Price on request
Request a quote for more information about this service.
Biology
Price on request
Request a quote for more information about this service.

Plant-Microbe Genomics Facility has not received any ratings.

Be the first to endorse Plant-Microbe Genomics Facility

Endorse this lab