1352612264550 7fffb663120dd173020ed4b9bc223583

Physiological Proteomics Core Facility

Indianapolis, Indiana, US

This facility enables researchers to identify and quantify proteins in simple or complex samples and characterize protein post-translational modifications or conjugates. Combining a new mass spectrometry-based analytical platform, IdentiQuantXL™, large format/high throughput gel-based proteomics, and experienced proteomic researchers, the PPCF provides unique approaches to biomarker discovery in health and disease, equipment and software for 1D or 2D gel analyses, Western blotting, high throughput differential expression proteomics with LC-MS/MS, identification/characterization of peptide and protein modifications, MS/MS of purified peptides and proteins, protein identification via LC-MS/MS of gel-plug or solution tryptic digests, targeted analyses of specific molecules with selected reaction monitoring (SRM), and proteomics methods development, consultation, and bioinformatic analysis of complicated results.

We... Show more »

This facility enables researchers to identify and quantify proteins in simple or complex samples and characterize protein post-translational modifications or conjugates. Combining a new mass spectrometry-based analytical platform, IdentiQuantXL™, large format/high throughput gel-based proteomics, and experienced proteomic researchers, the PPCF provides unique approaches to biomarker discovery in health and disease, equipment and software for 1D or 2D gel analyses, Western blotting, high throughput differential expression proteomics with LC-MS/MS, identification/characterization of peptide and protein modifications, MS/MS of purified peptides and proteins, protein identification via LC-MS/MS of gel-plug or solution tryptic digests, targeted analyses of specific molecules with selected reaction monitoring (SRM), and proteomics methods development, consultation, and bioinformatic analysis of complicated results.

We provide the following state-of-the-art mass spectrometry-based proteomics services:

Protein Identification
Protein Quantification
Differential Protein Expression Analysis
Protein Post-translational Modification Analysis
Bioinformatics and Pathway Analysis
Biomarker Discovery

Please note, a set up fee is applied when the number of samples is less than 10.

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Physiological Proteomics Core Facility has not listed any services.

Biomarker Discovery
Price on request
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LC-MS/MS
Biostatistics
Starting at $100.00 per hour
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Image Analysis
Starting at $100.00 per hour
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LC-MS
Liquid Chromatography-Coupled Mass Spectrometry
Price on request
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LTQ-MS
LTQ Linear Ion Trap Mass Spectrometry
Starting at $140.00 per sample

1D LC-MS/MS
Gel plug protein $140/sample
Gel plug protein + PTM $200/sample
Complex sample $400/injection
Complex sample + PTM $600/injection

2D LC-MS/MS (MuDPIT) (setup* fee $3000) $600/gradient step
(One gradient step is considered as one injection)

2D LC-MS/MS (MuDPIT) (setup* fee $3000) + PTM $800/gradient step
(One gradient step is considered as one injection)

1D LC-MS/MS
Gel plug protein $140/sample
Gel plug protein + PTM $200/sample
Complex sample $400/injection
Complex sample + PTM $600/injection

2D LC-MS/MS (MuDPIT) (setup* fee $3000) $600/gradient step
(One gradient step is considered as one injection)

2D LC-MS/MS (MuDPIT) (setup* fee $3000) + PTM $800/gradient step
(One gradient step is considered as one injection)

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Label-Free Mass Spectrometry
Starting at $600.00 per sample

IdentiQuantXL
1D LC-MS/MS Protein Identification, Quantification, and Statistics $600/injection
1D LC-MS/MS Protein Ident., Quant., and Stat. + PTM $800/injection
2D LC-MS/MS (MuDPIT) (setup* fee $3000) $800/gradient step
2D LC-MS/MS (MuDPIT) (setup* fee $3000) + PTM $1000/gradient step

IdentiQuantXL
1D LC-MS/MS Protein Identification, Quantification, and Statistics $600/injection
1D LC-MS/MS Protein Ident., Quant., and Stat. + PTM $800/injection
2D LC-MS/MS (MuDPIT) (setup* fee $3000) $800/gradient step
2D LC-MS/MS (MuDPIT) (setup* fee $3000) + PTM $1000/gradient step

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Membrane protein enrichment
Starting at $200.00 per sample
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MudPIT Analysis
Multidimensional Protein Identification Technology
Price on request
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Plasma protein depletion
Starting at $140.00 per sample

ProteoPrep20

ProteoPrep20

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PAGE
Polyacrylamide Gel Electrophoresis
Starting at $600.00 per sample

Sample Preparation (mandatory) $100/hr
1D gel (small format) $600/gel
2D gel (large format only; includes stain) (2 replicates/sample) $1,400/sample

Sample Preparation (mandatory) $100/hr
1D gel (small format) $600/gel
2D gel (large format only; includes stain) (2 replicates/sample) $1,400/sample

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Western Blot
Starting at $400.00 per gel

Western Immunostain for 1D gel (1o and 2o ab supplied by customer) $400/gel
Western Immunostain for 2D gel (1o and 2o ab supplied by customer) $600/gel

Western Immunostain for 1D gel (1o and 2o ab supplied by customer) $400/gel
Western Immunostain for 2D gel (1o and 2o ab supplied by customer) $600/gel

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Biology
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Biochemistry & Molecular Biology
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Protein Services
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Protein Expression Visualization
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Protein Sequencing
Price on request
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Protein Purification and Quantification
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Protein Depletion
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Imaging & Spectroscopy
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Spectroscopy
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Mass Spectrometry
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Tandem Mass Spectrometry Methods
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Liquid Chromatography Coupled Mass Spectrometry Methods
Price on request
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Specialty Mass Spectrometry Methods
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Chemistry and Materials
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Analytical Chemistry Services
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Purification Services
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Electrophoresis
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Gel Electrophoresis
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Pharmacology & Toxicology
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Bioanalysis
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Biomarkers
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Computational & Statistical Analysis
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Data Analysis and Management
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Biostatistics & Bioinformatics
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