**PhenoVista offers high content imaging services to further your COVID-19 research, including assays for viral uptake, organelle localization, autophagy and drug repurposing. Discounts available for COVID-19-related research. Click 'Request More Info' button on this page to ask about offer details.**
PhenoVista Biosciences offers cell-based imaging & assay development services for biopharma discovery needs in regard to screening (lead optimization), mechanism-of-action (MOA) studies and preclinical model characterization. PhenoVista accepts a broad range of biologies in 2D and 3D, with neuronal, inflammatory, muscular, metabolic and immuno-co-cultures being the biggest practice areas. We also offer investigative neurtoxicity studies for biopharma, pesticide and cosmetic development.
PhenoVista partners with leading suppliers of cell models (including organoids, primary human tumors and iPS-derived neurons) and suppliers of antibodies (and fluorescent dyes) to deliver a total assay development solution.
Our facilities include state-of-the-art wet labs with tissue culture and automated liquid handling capabilities. Our analytical capabilities include several best of brand automated fluorescence microscopes and high content screening (HCS) instruments coupled with proprietary analysis and visualization software developed in-house and with our technology partners.
PhenoVista delivers quantitative data and the ability to visualize the biology for a qualitative understanding of response. Multiplexed channels and parallel imaging palettes enable a thorough interrogation of protein expression, location, trafficking and cell morphology, where necessary.
Phenotypic screening or Phenotypic drug discovery (PPD) is an approach that measures functional characteristics of the model used, whether it be a whole organism or a cell culture. Functional characteristics may include the morphology of the cells, their ability to synthesize a particular molecule, or even their ability to proliferate. Essentially, phenotypic approaches treat the biological system as a black box where compounds are added and a change in the behavior of the model is quantified. Phenotypic imaging is complementary to to flow cytometry, and sometimes called "in-cell western."
Certification: Research grade services
Phenovista Biosciences offers customized High Content Screening (HCS) as well as multiparametric high content imaging assays and phenotype screening. Contact us to discuss what you would like your assay to measure.
High throughput imaging-based screens available in a wide range of biological systems including those using iPS-derived, ES and human primary cells.
MOA studies for clinical compounds or for studies intended for publication. Deliverables include methods, quantified responses, representative images and a selection of high-resolution, high magnification images. Any mammalian biology accepted. Current popular topics include therapies for autoimmune diseases and processes of degeneration/ageing. For clinical compounds, methods often developed using compound analogs, to avoid compulsion for legal discovery, until the project is derisked.
Investigative neurotoxicity predicted with neurite outgrowth, mitochondrial health and autophagy response measurements, in primary rat or iPS-derived human models (sourced from partners in our supply chain).
Measure uptake of biomolecules such as therapeutic antibodies, proteins or fluorescently-labelled small molecules by mammalian cells (cell lines, primary cells or iPS-derived lines). Receive quantification of overall uptake as well as the candidate molecules distribution across subcellular structures such as nucleus, nucleolus, mitochondria, lysosomes, Golgi and plasma membrane.
Turnaround 4 weeks.
Organoids and spheroids sourced from partners, treated with customer drugs and response imaged with multiplexed markers. Models include brain, liver, gut and pancreas/ islets, with more continuously being developed by our suppliers. Imaged outputs include morphology & structure, differentiation markers, immune infiltration, and cell health.
Quantitative imaging based assay measuring structural and metabolic end-points in response to reference stressors. Multiple cell types available.
Activated PBMCs, imaged with Ab markers, added to spheroid culture, with invasion measured with fluorescence imaging.
Imaging-based analysis of apoptotic marker expression in a variety of cell types.
Quantitative imaging based assay using cell lines or iPS-derived neurons. Measurement of endpoints for mitochondrial structure and function, apoptosis, necrosis and neurite morphology. Custom endpoints available upon request
Wound healing and chemical gradient based analyses of cell migration. Various cell types available including B and T cells, endothelial cells and epithelial cells.
Measure outgrowth of neurites in a variety of supported cell types including PC-12 and iPS-derived neurons.
Using proprietary technology from Persomics Inc we are the only certified provider of genome wide RNAi combined with imaging-based phenotypic assays.
Imaging using widefield microscopes, adherent and suspension cells supported.
Available by experts with decades of experience using a variety of imaging platforms, analysis software packages and data visualization approaches.
Quantify dynamic cellular processes using this classical approach.
Custom biomarker analysis of adherent cell types using brightfield or fluorescence microscopy. Quantitative analysis available.
Various levels of assay complexity from simple dye-exclusion to annexin V and other apoptotic markers. Adherent and suspension cells supported.
Analysis of cell migration during wound healing in scalable imaging-based format. Various cell types supported.
Deconvolution of widefield, confocal, multiphoton and other imaging modalities using Huygens software. Quantitative analysis and 3D visualization available.
Confocal imaging of fluorescent samples. Quantitative analysis available.
Antibody staining of adherent cells and fluorescence imaging. Quantitative analysis available.
Various cell based models including human primary cells and cell lines used to monitor metabolic pathways and energy utilization/storage. All assays are performed in 384 well plates and are fully customizable. Turnaround is 4-8 weeks.
Custom development of phenotypic and hybrid assays that incorporate target-based readouts. Fully quantitative and scalable. $1000
Optimization of cell type, culture conditions, antibodies and controls. Development and validation of assay methodology including imaging, image analysis and data analysis. All assays are performed in 384 well plates and fully scalable. Turnaround time highly dependent on assay complexity but typically 2-10 weeks with regular updates and milestones. Pricing discounted aggressively for customers who commit to a screening project based on the developed assay. Starting at $5000.
iPS-derived neurons and co-culture options. Multiple measurements available related to CNS diseases. All assays are performed in 384 well plates and fully scalable. Turnaround is 8 weeks for up to 1000 compounds with dose-response profiling. Custom development options are available.
Custom phenotypic analyses of iPS cell differentiation using high content imaging and customer-defined outputs. Common markers of pluripotency can be combined with functional and morphological outputs. Dose-response testing of up to 1000 compounds within 8-12 weeks depending on assay design. All assays are performed in 384 well plates and fully scalable.
Custom art work made to order using cells and stains of choice - perfect for your company's lobby or your office wall. Contact us for options.
Human iPS-derived neurons, high resolution phenotypic assay that is fully customizable to a range of disease models. This assay is performed in 384 well plates and is fully scalable.Turnaround within 8-12 weeks.
Lead Identification and Validation Services
"Had a great experience with PhenoVista. They were rigorous, timely and collaborative in setting up and optimizing a HCA assay for us. They really went out of their way to make sure we discussed all the controls and conditions to ensure the experiment got run exactly the way we wanted it to. They have wealth of experience and expertise in HCA and they had very helpful suggestions. I would highly recommend them, and I will use them again myself."
We have worked with PhenoVista on a project that involved developing a new cell-based assay. They took initial feedback and our internal work and developed it into a standardized assay that allowed us to compare and discriminate across a range of test articles, working closely with us to setup the new assay and ensuring that project goals were met. They generated images that helped internally communicate the findings in a compelling way and quantification that helped make decisions. We are continuing to work with them to increase the complexity of the assays, and would recommend them for similar ‘non-traditional’ projects in the future.
We have been working with PhenoVista Biosciences for a special project. It has been a pleasure working with them from the first moment of contact to the end of the service provided. What really sticks out to us is the professional service quality that is coupled to with an excellent quality of scientific depth in what they do. When you partner with PhenoVista Biosciences you are partnering with fully engaged scientists. They were able to trouble shoot to improve results and engage us as they did so. This gave us confidence in their abilities and we were overly pleased with their ability do deliver at a high level, beyond our initial expectations. We recommend PhenoVista Biosciences with full confidence.
I discovered PhenoVista Biosciences in a search for a lab that could high-throughput neuron toxicity screening assays with iCell Neurons. I am really happy with the throughput
and results we received from them for our initial screen with the ICell Neurons and look forward to conducting more work with this lab. Really appreciate the one-on-one teleconferences and study design help that they offer. I recommend them to researchers needing an outsourcing option to run assays for in vitro neurotoxicity.
I discovered PhenoVista thru Science Exchange, when they responded with a quote for a project I posted. Boy am I glad they did! They were extremely knowledgeable and made several great suggestions for setting up the assay. They quickly grasped the scientific question we were asking and interacted as collaborators more than as a "CRO". The work was done on time and included all the controls and conditions required to make a clear decision. They had great images, masked perfectly for good analysis.
I highly recommend them to anyone considering a study in their area. I will definitely use them again when possible.