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Pharmatest Services Ltd

Turku, Finland, FI

Pharmatest Services Ltd (Pharmatest) is a contract research organization (CRO) specialized in clinically predictive preclinical efficacy services in the therapeutic areas of oncology and skeletal diseases.

Our services have been developed with just one goal in mind – to make your nonclinical drug development faster and more predictive. Our mission is to reduce the amount of drug candidates failing in clinical trials. Outsource your preclinical efficacy testing to Pharmatest and increase significantly the speed of your drug development with reduced costs.

Partnering with Pharmatest gives you an on-demand access to latest translational tools for lead validation and proof-of-concept studies in oncology and skeletal diseases.

Selected Publications

  • Valta M, Fagerlund K, Suominen M, Halleen J, Tuomela J 2015 Importance of microenvironment in preclinical models of breast and prostate cancer. *World J... Show more »

Pharmatest Services Ltd (Pharmatest) is a contract research organization (CRO) specialized in clinically predictive preclinical efficacy services in the therapeutic areas of oncology and skeletal diseases.

Our services have been developed with just one goal in mind – to make your nonclinical drug development faster and more predictive. Our mission is to reduce the amount of drug candidates failing in clinical trials. Outsource your preclinical efficacy testing to Pharmatest and increase significantly the speed of your drug development with reduced costs.

Partnering with Pharmatest gives you an on-demand access to latest translational tools for lead validation and proof-of-concept studies in oncology and skeletal diseases.

Selected Publications

  • Valta M, Fagerlund K, Suominen M, Halleen J, Tuomela J 2015 Importance of microenvironment in preclinical models of breast and prostate cancer. World J Pharmacol 4(1):47-57.
  • Bernoulli J, Konkol Y, Vuorikoski H, Yatkin E 2014 Effects of afala and antiestrogen ICI 182,780 in the model of hormone-dependent prostate inflammation. Bull Exp Biol Med 156(6):807-9.
  • Suominen MI, Rissanen JP, Käkönen R, Fagerlund KM, Alhoniemi E, Mumberg D, Ziegelbauer K, Halleen JM, Käkönen SM, Scholz A 2013 Survival Benefit With Radium-223 Dichloride in a Mouse Model of Breast Cancer Bone Metastasis. J Natl Cancer Inst 105(12):908-16.
  • Erlandsson MC, Svensson MD, Jonsson IM, Bian L, Ambartsumian N, Andersson S, Peng Z, Vääräniemi J, Ohlsson C, Andersson KM, Bokarewa MI 2013 Expression of metastasin S100A4 is essential for bone resorption and regulates osteoclast function. Biochim Biophys Acta 1833(12):2653-63.
  • Strube A, Suominen MI, Rissanen JP, Mumberg D, Klar U, Halleen JM, Käkönen SM 2011 The anti-tumor agent sagopilone shows antiresorptive effects both in vitro and in vivo. Osteoporos Int 22:2887–93.
  • Neerup TS, Stahlhut M, Petersen JS, Daugaard JR, Jensen JE, Peng Z, Morko J, Thorkildsen C 2011 ZP2307, a novel, cyclic PTH(1-17) analog that augments bone mass in ovariectomized rats. Bone 48:1319-27.
  • Rissanen JP, Halleen JM 2010 Models and screening assays for drug development in osteoporosis. Expert Opin Drug Discov 5:1163-74.
  • Rissanen JP, Ylipahkala H, Fagerlund KM, Long C, Väänänen HK, Halleen JM 2009 Improved methods for testing antiresorptive compounds in human osteoclast cultures. J Bone Miner Metab 27:105-9.
  • Rissanen JP, Suominen MI, Peng Z, Morko J, Rasi S, Risteli J, Halleen JM 2008 Short-term changes in serum PINP predict long-term changes in trabecular bone in the rat ovariectomy model. Calcif Tissue Int 82:155-61.
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Pharmatest Services Ltd has not listed any services.

In vitro Oncology Models
Price on request
  • Cancer cell proliferation and viability assays
  • Cancer cell apoptosis and cytotoxicity assays
  • Cancer cell invasion assays

Pharmatest offers in vitro cancer cell assays that can be used for fast demonstration of efficacy of test compounds. Cell proliferation and viability can be determined using CellTiter-GloTM... Show more »

  • Cancer cell proliferation and viability assays
  • Cancer cell apoptosis and cytotoxicity assays
  • Cancer cell invasion assays

Pharmatest offers in vitro cancer cell assays that can be used for fast demonstration of efficacy of test compounds. Cell proliferation and viability can be determined using CellTiter-GloTM luminescent cell viability assay. Cytotoxicity can be determined using the CellToxTM Green fluorescence-based cytotoxicity assay, and apoptosis can be measured using several methods. Cell invasion is determined using a system where cancer cells are grown on a basement membrane-like MatrigelTM coated filter chamber, and their capacity to migrate through the Matrigel layer is determined.

We offer studies from high-throughput screening of a large number of test compounds to extensive studies that will confirm the efficacy and allow calculating EC50 values for the test compounds. The extensive studies will demonstrate what cancer cell lines are affected by the test compounds, and the efficacy can then be confirmed in vivo in our animal models using the same cancer cell lines that were affected by the test compounds in vitro.

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Cancer Animal Models
Price on request
  • Breast cancer (with MDA-MB-231(SA), 4T1, BT-474, MCF-7 cells)
  • Prostate cancer (with LNCaP, VCaP, PC-3 cells)
  • Multiple myeloma (with 5TGM1 cells)
  • Pancreatic cancer (with BxPC3 cells)

In oncology Pharmatest is specialized in orthotopic and metastasis models, and our special expertise is in bone metastasis models. The... Show more »

  • Breast cancer (with MDA-MB-231(SA), 4T1, BT-474, MCF-7 cells)
  • Prostate cancer (with LNCaP, VCaP, PC-3 cells)
  • Multiple myeloma (with 5TGM1 cells)
  • Pancreatic cancer (with BxPC3 cells)

In oncology Pharmatest is specialized in orthotopic and metastasis models, and our special expertise is in bone metastasis models. The major advantage of these models is the proper tissue microenvironment that is known to affect tumor growth, metastasis and drug resistance. This increases substantially the clinical relevance and predictivity over traditionally used subcutaneous models that lack the proper microenvironment.

Pharmatest offers both xenograft and syngeneic cancer models in mice. Xenograft models include using human cancer cell lines, which requires using immunocompromised mice. Syngeneic models include using mouse cell lines that allow using mice with normal immune system, allowing to test immunomodulators. Pharmatest is also developing novel models using humanized mice that have human immune system, allowing to test immunomodulators using human cancer cells.

Pharmatest can also assist our customers in finding and selecting appropriate patient-derived xenograft (PDX) models from the many providers of PDX models. Our experts will find the best PDX models for the purpose of the customer, and Pharmatest can manage the projects. The customer needs only to contact and communicate with Pharmatest, and Pharmatest will subcontract the PDX studies to the providers, take care of all correspondence with the PDX providers, and prepare a summary report including all results for the customer.

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In vitro Osteoporosis Models
Price on request
  • Osteoclast differentiation assay
  • Osteoclast activity assay (bone resorption assay)
  • Osteoblast differentiation assay
  • Osteoblast activity assay (bone formation assay)

Pharmatest’s in vitro bone cell culture assays that can be used for fast demonstration of efficacy of test compounds. We offer studies from preliminary... Show more »

  • Osteoclast differentiation assay
  • Osteoclast activity assay (bone resorption assay)
  • Osteoblast differentiation assay
  • Osteoblast activity assay (bone formation assay)

Pharmatest’s in vitro bone cell culture assays that can be used for fast demonstration of efficacy of test compounds. We offer studies from preliminary testing of a large number of test compounds to extensive testing that will confirm the efficacy and allow calculating EC50 values for the test compounds.

Our bone cell culture assays can be conveniently used for identifying compounds with best efficacy for subsequent testing in animal studies. They include assays for demonstrating direct anabolic effects on activity or differentiation of bone forming osteoblasts and direct anti-resorptive effects on activity or differentiation of bone resorbing osteoclasts.

Osteoclast assays are available in human, rat and mouse cells. Human osteoclast cultures are performed using CD34+ human osteoclast precursor cells. Osteoclast differentiation is quantitated by measuring tartrate-resistant acid phosphatase isoform 5b activity (TRACP5b) released into the culture medium, and bone resorption activity of the formed mature osteoclasts is quantitated by measuring C-terminal cross-linked telopeptides of type I collagen (CTX-I) released into the culture medium.

Osteoblast assays are performed using the mouse mesenchymal progenitor cell line KS483 that is allowed to differentiate into mature osteoblasts capable of forming bone nodules. Osteoblast differentiation is quantitated by measuring alkaline phosphatase activity from the cell lysates, and bone formation activity of the formed mature osteoblasts is quantitated by measuring procollagen I N-terminal propeptide (PINP) released into the culture medium.

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Osteoporosis Animal Models
Price on request
  • Rat and mouse ovariectomy (OVX) models of postmenopausal osteoporosis
  • Rat and mouse orchidectomy (ORX) models of male osteoporosis
  • Mouse model of glucocorticoid-induced osteoporosis (GIO)

For the follow-up and endpoint measurements available in these models please check the Service “Bone analysis”.

With the... Show more »

  • Rat and mouse ovariectomy (OVX) models of postmenopausal osteoporosis
  • Rat and mouse orchidectomy (ORX) models of male osteoporosis
  • Mouse model of glucocorticoid-induced osteoporosis (GIO)

For the follow-up and endpoint measurements available in these models please check the Service “Bone analysis”.

With the osteoporosis animal models Pharmatest offers specially designed study setups for testing anti-catabolic and anabolic compounds in prevention and treatment settings. Our projects can range from less extensive preliminary studies to regulatory studies performed according to the guidelines of FDA and EMA. We have also designed special project types for testing biosimilars and functional foods that have less extensive regulatory requirements.

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Osteoarthritis Animal Models
Price on request
  • Rat monoiodoacetate (MIA) model
  • Rat medial meniscal tear and medial collateral ligament transection (MMT+MCLT) model
  • Rat and rabbit anterior cruciate ligament transection (ACLT) models
  • Rabbit partial medial meniscectomy (pMMx) model
  • Rat ACLT and pMMx model

Osteoarthritis (OA) models of Pharmatest include models... Show more »

  • Rat monoiodoacetate (MIA) model
  • Rat medial meniscal tear and medial collateral ligament transection (MMT+MCLT) model
  • Rat and rabbit anterior cruciate ligament transection (ACLT) models
  • Rabbit partial medial meniscectomy (pMMx) model
  • Rat ACLT and pMMx model

Osteoarthritis (OA) models of Pharmatest include models where OA is induced chemically (Rat MIA model) or surgically (MMT+MCLT, ACLT, pMMx, and ACLT+pMMx models). The MIA model is considered a model of OA-induced pain, while the surgical models are more suitable for studying effects on OA-related cartilage and bone destruction.

Follow-up and endpoint measurements available include pain measurements and cartilage and bone analyses with various methods. One key endpoint measurement is microscopic evaluation of degenerative changes in operated knee joints according to the recommendations of the Osteoarthritis Research Society International (OARSI) histopathology initiative.

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Bone Safety Studies
Price on request
  • Intact rats and mice
  • Rat and mouse ovariectomy (OVX) models
  • Rat and mouse orchidectomy (ORX) models
  • Mouse model of glucocorticoid-induced osteoporosis (GIO)
  • Characterization of bone phenotype in genetically modified mice

Bone health is affected by several factors, including age, physical activity, diseases,... Show more »

  • Intact rats and mice
  • Rat and mouse ovariectomy (OVX) models
  • Rat and mouse orchidectomy (ORX) models
  • Mouse model of glucocorticoid-induced osteoporosis (GIO)
  • Characterization of bone phenotype in genetically modified mice

Bone health is affected by several factors, including age, physical activity, diseases, medication, various hormonal, lifestyle and nutrition conditions, and genetic defects and modifications. All these factors may induce either beneficial or adverse effects on bone safety. Pharmatest can help you determine bone safety of your new drug candidates, biosimilars, functional foods, nutrition supplements and other substances.

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Animal Bone Analysis
Price on request

Pharmatest’s standard study setups in different disease models include different combinations of the following bone analysis methods that can also be used as stand-alone services, where you just send us your samples and we will perform the selected measurements and provide you with the results:

• Micro-computed tomography... Show more »

Pharmatest’s standard study setups in different disease models include different combinations of the following bone analysis methods that can also be used as stand-alone services, where you just send us your samples and we will perform the selected measurements and provide you with the results:

• Micro-computed tomography (µCT)
• Peripheral quantitative computed tomography (pQCT)
• Dual-Energy X-Ray Absorptiometry (DXA)
• Radiographic imaging and image analysis
• Full-service bone histomorphometry, including static and dynamic parameters
• Processing of tissue samples for histological analysis, including tissue preparation and embedding to paraffin or plastic, and a variety of histological staining techniques
• Bone ash weight analysis
• Bone biomechanical testing, including cantilever bending, three-point bending, and compression tests
• Biochemical markers of bone turnover, including PINP, osteocalcin, CTX-I and TRACP 5b

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Clinical Biomarkers
Price on request
  • Biochemical markers of bone turnover: PINP, CTX-I, BAP, TRACP5b, osteocalcin
  • Markers of calcium metabolism: 25-Hydroxy Vitamin D, PTH

The measurements are performed in serum samples using the IDS-iSYS automated platform (IDS, Boldon, UK). We also offer measurements of other bone-related biomarkers using manual methods (please ask for further information).

  • Biochemical markers of bone turnover: PINP, CTX-I, BAP, TRACP5b, osteocalcin
  • Markers of calcium metabolism: 25-Hydroxy Vitamin D, PTH

The measurements are performed in serum samples using the IDS-iSYS automated platform (IDS, Boldon, UK). We also offer measurements of other bone-related biomarkers using manual methods (please ask for further information).

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In vitro Hormone Function Models
Price on request
  • Androgenic assay using LNCaP human prostate cancer cells
  • Estrogenic assay using mouse mesenchymal progenitor cell line KS483

The in vitro androgenic assay is performed using the PSA-expressing androgen-sensitive human prostate cancer cell line LNCaP. Cell proliferation is determined using CellTiter-GloTM luminescent cell... Show more »

  • Androgenic assay using LNCaP human prostate cancer cells
  • Estrogenic assay using mouse mesenchymal progenitor cell line KS483

The in vitro androgenic assay is performed using the PSA-expressing androgen-sensitive human prostate cancer cell line LNCaP. Cell proliferation is determined using CellTiter-GloTM luminescent cell viability assay (CTG). Secreted PSA levels in the conditioned medium can also be measured.

The in vitro estrogenic assay is performed using the mouse mesenchymal progenitor cell line KS483 that is allowed to differentiate into mature osteoblasts capable of forming bone nodules. Osteoblast formation is quantitated by measuring alkaline phosphatase activity from the cell lysates, and bone formation activity of the formed mature osteoblasts is quantitated by measuring procollagen I N-terminal propeptide (PINP) released into the culture medium.

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Hormone Function Animal Models
Price on request
  • OECD validated Hershberger bioassay for determining androgenic effects
  • OECD validated Uterotrophic bioassay for determining estrogenic effects

The Hershberger bioassay is designed to identify suspected androgens and antiandrogens (either drug candidates or endocrine disruptors) based on changes in the weights of five... Show more »

  • OECD validated Hershberger bioassay for determining androgenic effects
  • OECD validated Uterotrophic bioassay for determining estrogenic effects

The Hershberger bioassay is designed to identify suspected androgens and antiandrogens (either drug candidates or endocrine disruptors) based on changes in the weights of five androgen-responsive tissues. The uterotrophic bioassay identifies estrogenic or anti-estrogenic compounds (either drug candidates or endocrine disruptors) based on their effects on wet and blotted uterus weight.

There are several advantages in using these OECD-validated assays:
- The weighted target tissues are the natural targets for androgens/estrogens
- The tissue growth response is relatively rapid
- The tissue weights are quantitative

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Custom Animal Models
Price on request
  • Co-development of novel models with a customer, funded by the customer
  • The investment is returned as price reductions when the new model is used by the customer
  • Pharmatest is allowed to offer the developed model freely to our other customers
  • Long-term outcome: An entire R&D department available for our customers... Show more »
  • Co-development of novel models with a customer, funded by the customer
  • The investment is returned as price reductions when the new model is used by the customer
  • Pharmatest is allowed to offer the developed model freely to our other customers
  • Long-term outcome: An entire R&D department available for our customers free of charge!

Model development is also available for exclusive use for the funding customer (please ask for details).

All co-development projects receive a top priority in our R&D pipeline.

Even though we keep a constant eye on the early drug discovery field and do our best to maintain a broad and ranging service portfolio, you may find that you need a particular model that we do not have. In a case like this, we offer you an option for a co-development project. This is a concept we have used with great success to provide our customers with a fast-track access to completely new models.

Those customers who have extensive use for one of our models can also in-license that particular model or assay. Instead of working with us on a project basis, we can transfer the entire model to your laboratory, provide you with all necessary documentation (SOPs) related to the model, including all future updates, and train your personnel to use the model according to the SOPs.

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