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Pharmatest Services Ltd

2 Orders Completed
Turku, FI

About Pharmatest Services Ltd

Pharmatest Services Ltd (Pharmatest) is a contract research organization (CRO) specialized in clinically predictive preclinical efficacy services in the therapeutic areas of oncology and skeletal diseases.

Our services have been developed with just one goal in mind – to... Show more »

Pharmatest Services Ltd (Pharmatest) is a contract research organization (CRO) specialized in clinically predictive preclinical efficacy services in the therapeutic areas of oncology and skeletal diseases.

Our services have been developed with just one goal in mind – to make your preclinical drug development faster and more clinically predictive. Our mission is to reduce the amount of drug candidates failing in clinical trials. Outsource your preclinical efficacy testing to Pharmatest and increase significantly the speed of your drug development with reduced costs.

**Selected Publications**

-       Kähkönen TE, Suominen MI, Mäki-Jouppila JHE, Halleen JM, Tanaka A, Seiler M, Bernoulli J 2019 Human Immune System Increases Breast Cancer-Induced Osteoblastic Bone Growth in a Humanized Mouse Model without Affecting Normal Bone. J Immunol Res 2019:4260987.
-       Kähkönen TE, Bernoulli J, Halleen JM, Suominen MI 2019 Novel and Conventional Preclinical Models to Investigate Bone Metastasis. Curr Mol Biol Rep 5(1):48-54 (invited review article).
-       Juzeniene A, Bernoulli J, Suominen M, Halleen J, Larsen RH 2018 Antitumor Activity of Novel Bone-seeking, α-emitting 224Ra-solution in a Breast Cancer Skeletal Metastases Model. Anticancer Res 38(4):1947-1955.
-       Suominen MI, Fagerlund KM, Rissanen JP, Konkol Y, Morko J, Peng Z, Alhoniemi E, Laine S, Corey E, Mumberg D, Ziegelbauer K, Käkönen S, Halleen JM, Vessella  R, Scholz A 2017 Radium-223 Inhibits Osseous Prostate Cancer Growth by Dual Targeting of Cancer Cells and Bone Microenvironment in Mouse Models. Clin Cancer Res 23(15):4335-46.
-       Valta M, Fagerlund K, Suominen M, Halleen J, Tuomela J 2015 Importance of microenvironment in preclinical models of breast and prostate cancer. World J Pharmacol 4:47-57 (invited review article).
-       Suominen MI, Rissanen JP, Käkönen R, Fagerlund KM, Alhoniemi E, Mumberg D, Ziegelbauer K, Halleen JM, Käkönen SM, Scholz A 2013 Survival benefit with radium-223 dichloride in a mouse model of breast cancer bone metastasis. J Natl Cancer Inst 105:908-916.
-       Rissanen JP, Halleen JM 2010 Models and screening assays for drug discovery in osteoporosis. Expert Opin Drug Discov 5:1163-1174 (invited review article).
-       Rissanen JP, Ylipahkala H, Fagerlund KM, Long C, Väänänen HK, Halleen JM 2009 Improved methods for testing antiresorptive compounds in human osteoclast cultures. J Bone Miner Metab 27:105-109.
-       Rissanen JP, Suominen MI, Peng Z, Morko J, Rasi S, Risteli J, Halleen JM 2008 Short-Term Changes in Serum PINP Predict Long-Term Changes in Trabecular Bone in the Rat Ovariectomy Model. Calcif Tissue Int 82:155-161.
-       Rissanen JP, Suominen MI, Peng Z, Halleen JM 2008 Secreted Tartrate-Resistant Acid Phosphatase 5b is a Marker of Osteoclast Number in Human Osteoclast Cultures and the Rat Ovariectomy Model. Calcif Tissue Int 82:108-115.
 

 

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Our Services (65)


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Osteogenesis Imperfecta Animal Models

Price on request

- Transgenic mouse model with G610C mutation in Col1a2 gene

Human Osteogenesis Imperfecta (OI) is a group of genetic disorders that results in heterogeneous clinical symptoms ranging from low bone mass and occasional fractures to perinatal lethality. Most cases of OI are caused by autosomal dominant mutations in the... Show more »

- Transgenic mouse model with G610C mutation in Col1a2 gene

Human Osteogenesis Imperfecta (OI) is a group of genetic disorders that results in heterogeneous clinical symptoms ranging from low bone mass and occasional fractures to perinatal lethality. Most cases of OI are caused by autosomal dominant mutations in the genes encoding type I collagen (COL1A1 and COL1A2) and are classified in subtypes I to IV.

Most OI animal models include gene-modified mice mimicking genetic disorders observed in human patients. Pharmatest offers a mouse model of OI where ‘Amish variant’ mice with a G610C mutation in the Col1a2 gene are used. Heterozygous mice (+/G610C) mimic human type IV OI with a mild-to-moderate severity and exhibit decreased bone mineral density and bone strength.

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In vitro Oncology Models

Price on request

- Cancer cell proliferation and viability assays

- Cancer cell apoptosis and cytotoxicity assays

- Cancer cell invasion assays

Pharmatest offers in vitro cancer cell assays that can be used for fast demonstration of efficacy of test compounds. Cell proliferation and viability can be determined using the... Show more »

- Cancer cell proliferation and viability assays

- Cancer cell apoptosis and cytotoxicity assays

- Cancer cell invasion assays

Pharmatest offers in vitro cancer cell assays that can be used for fast demonstration of efficacy of test compounds. Cell proliferation and viability can be determined using the CellTiter-GloTM luminescent cell viability assay. Cytotoxicity is determined using the CellToxTM Green fluorescence -based cytotoxicity assay, and apoptosis can be measured using several methods. Cell invasion is determined using a system where cancer cells are grown on a basement membrane-like MatrigelTM coated filter chamber, and their capacity to migrate through the Matrigel layer is determined.

We offer studies from preliminary testing of a large number of test compounds to extensive studies that will confirm the efficacy and allow calculating EC50 values for the test compounds. The extensive studies will demonstrate what cancer cell lines are affected by the test compounds, and the efficacy can then be confirmed in vivo in our animal models using the same cancer cell lines that were affected by the test compounds in vitro.

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Oncology Animal Models

Price on request

-          Breast cancer (with MDA-MB-231(SA), 4T1, BT-474, MCF-7 cells)

-          Prostate cancer (with LNCaP, 22Rv1, VCaP, PC-3 cells)

-          Multiple myeloma (with 5TGM1... Show more »

-          Breast cancer (with MDA-MB-231(SA), 4T1, BT-474, MCF-7 cells)

-          Prostate cancer (with LNCaP, 22Rv1, VCaP, PC-3 cells)

-          Multiple myeloma (with 5TGM1 cells)

-          Pancreatic cancer (with BxPC3, Capan-1, Panc-1, MiaPaCa-2 cells)

In oncology Pharmatest is specialized in orthotopic and metastasis models, and we have special expertise in modeling of bone metastases. The major advantage of these models is the proper tissue microenvironment that is known to affect tumor growth and drug resistance. This increases substantially the clinical relevance and predictivity over traditionally used subcutaneous models that lack the clinically relevant tissue microenvironment.

Pharmatest offers xenograft models in immunodeficient mice, syngeneic models in immunocompetent mice, and xenograft models in humanized mice. Xenograft models include human cancer cell lines that cannot be used in mice that have the normal mouse immune system. Syngeneic models include mouse cell lines that allow using mice with normal immune system, allowing to test immunomodulators. Humanized mice have human immune system, allowing to test immunotherapies targeting human cancer and/or immune cells.

Tumor growth can be monitored by IVIS (when luciferase labelled cancer cells are used) or by serum tumor biomarker measurements. Additionally, in bone metastasis models tumor-induced changes in bone can be measured by X-ray and µCT imaging, and by analysis of serum bone turnover markers. Endpoint measurements can include histology and immunohistochemical stainings.

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In vitro Osteoporosis Models

Price on request

-          Osteoclast differentiation assay

-          Osteoclast activity assay

-          Osteoblast differentiation... Show more »

-          Osteoclast differentiation assay

-          Osteoclast activity assay

-          Osteoblast differentiation assay

-          Osteoblast activity assay

Pharmatest’s in vitro bone cell culture assays can be used for fast demonstration of efficacy of test compounds. We offer studies from preliminary testing of a large number of test compounds to extensive testing that will confirm the efficacy and allow calculating EC50 values for the test compounds.

Osteoclast assays are available in human, rat and mouse cells. Human osteoclast cultures are performed using CD34+ human osteoclast precursor cells. Osteoblast assays are performed using the mouse mesenchymal progenitor cell line KS483 that is allowed to differentiate into mature osteoblasts capable of forming bone nodules.

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Osteoporosis Animal Models

Price on request

-          Rat and mouse ovariectomy (OVX) models of postmenopausal osteoporosis

-          Rat and mouse orchidectomy (ORX) models of male osteoporosis

-          Mouse model of... Show more »

-          Rat and mouse ovariectomy (OVX) models of postmenopausal osteoporosis

-          Rat and mouse orchidectomy (ORX) models of male osteoporosis

-          Mouse model of glucocorticoid-induced osteoporosis (GIO)

For the follow-up and endpoint measurements available in these models please check the Service “Animal bone analysis”.

With the osteoporosis animal models Pharmatest offers specially designed study setups for testing anti-catabolic and anabolic compounds in prevention and treatment settings. Our projects can range from less extensive preliminary studies to regulatory studies performed according to the guidelines of FDA and EMA. We have also designed special project types for testing biosimilars and functional foods that have less extensive regulatory requirements.

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Osteoarthritis Animal Models

Price on request

-          Rat monoiodoacetate (MIA) model

-          Rat medial meniscal tear and medial collateral ligament transection (MMT+MCLT) model

-          Rat and rabbit anterior... Show more »

-          Rat monoiodoacetate (MIA) model

-          Rat medial meniscal tear and medial collateral ligament transection (MMT+MCLT) model

-          Rat and rabbit anterior cruciate ligament transection (ACLT) models

-          Rabbit partial medial meniscectomy (pMMx) model

-          Rat ACLT and pMMx model

Osteoarthritis (OA) models of Pharmatest include models where OA is induced chemically (MIA model) or surgically (MMT+MCLT, ACLT, pMMx, and ACLT+pMMx models).  The MIA model is considered a model of OA-induced pain, while the surgical models are more suitable for studying effects on OA-related cartilage and bone destruction.

Follow-up and endpoint measurements available include pain measurements and cartilage and bone analyses with various methods. One key endpoint measurement is microscopic evaluation of degenerative changes in operated knee joints according to the recommendations of the Osteoarthritis Research Society International (OARSI) histopathology initiative.

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Bone Safety Studies

Price on request

-          Intact rats and mice

-          Rat and mouse ovariectomy (OVX) models

-          Rat and mouse orchidectomy (ORX)... Show more »

-          Intact rats and mice

-          Rat and mouse ovariectomy (OVX) models

-          Rat and mouse orchidectomy (ORX) models

-          Mouse model of glucocorticoid-induced osteoporosis (GIO)

-          Characterization of bone phenotype in genetically modified mice

Many therapies are associated with harmful effects on bone. Pharmatest can help you determine bone safety of your new drug candidates, biosimilars, functional foods, nutrition supplements and other substances.

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Animal Bone Analysis

Price on request

Pharmatest’s standard study setups in different disease models include different combinations of the following bone analysis methods that can also be used as stand-alone services, where you just send us your samples and we will perform the selected measurements and provide you with the results:

- Micro-computed... Show more »

Pharmatest’s standard study setups in different disease models include different combinations of the following bone analysis methods that can also be used as stand-alone services, where you just send us your samples and we will perform the selected measurements and provide you with the results:

- Micro-computed tomography (µCT)
- Peripheral quantitative computed tomography (pQCT)
- Dual-Energy X-Ray Absorptiometry (DXA)
- Radiographic imaging and image analysis
- Full-service bone histomorphometry, including static and dynamic parameters
- Processing of tissue samples for histological analysis, including tissue preparation and embedding to paraffin or plastic, and a variety of histological staining techniques
- Bone ash weight analysis
- Bone biomechanical testing, including cantilever bending, three-point bending, and compression tests
- Biochemical markers of bone turnover, including PINP, osteocalcin, CTX-I and TRACP 5b

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Clinical Biomarkers

Price on request

·         Biochemical markers of bone turnover: PINP, CTX-I, BAP, TRACP5b, osteocalcin

·         Markers of calcium metabolism: 25-Hydroxy Vitamin D, PTH

The measurements are performed using the IDS-iSYS automated... Show more »

·         Biochemical markers of bone turnover: PINP, CTX-I, BAP, TRACP5b, osteocalcin

·         Markers of calcium metabolism: 25-Hydroxy Vitamin D, PTH

The measurements are performed using the IDS-iSYS automated platform (IDS, Boldon, UK). We also offer other measurements available in the IDS-iSYS platform, and other bone-related biomarkers using manual methods (please ask for further information).

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In vitro Hormone Function Models

Price on request

-          Androgenic assay using LNCaP human prostate cancer cells

-          Estrogenic assay using mouse mesenchymal progenitor cell line KS483

The in vitro androgenic assay is performed using the PSA-expressing... Show more »

-          Androgenic assay using LNCaP human prostate cancer cells

-          Estrogenic assay using mouse mesenchymal progenitor cell line KS483

The in vitro androgenic assay is performed using the PSA-expressing androgen-sensitive human prostate cancer cell line LNCaP. Cell proliferation is determined using CellTiter-GloTM luminescent cell viability assay. Secreted PSA levels in the conditioned medium can also be measured.

The in vitro estrogenic assay is performed using the mouse mesenchymal progenitor cell line KS483 that is allowed to differentiate into mature osteoblasts capable of forming bone nodules. Osteoblast formation is quantitated by measuring alkaline phosphatase activity from the cell lysates, and bone formation activity of the formed mature osteoblasts is quantitated by measuring procollagen I N-terminal propeptide (PINP) released into the culture medium.

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Hormone Function Animal Models

Price on request

-          OECD validated Hershberger bioassay for determining androgenic effects

-          OECD validated Uterotrophic bioassay for determining estrogenic effects

The Hershberger bioassay is designed to identify suspected... Show more »

-          OECD validated Hershberger bioassay for determining androgenic effects

-          OECD validated Uterotrophic bioassay for determining estrogenic effects

The Hershberger bioassay is designed to identify suspected androgens and antiandrogens (either drug candidates or endocrine disruptors) based on changes in the weights of five androgen-responsive tissues. The uterotrophic bioassay identifies estrogenic or anti-estrogenic compounds (either drug candidates or endocrine disruptors) based on their effects on wet and blotted uterus weight.   

There are several advantages in using these OECD-validated assays:

-          The weighted target tissues are the natural targets for androgens/estrogens

-          The tissue growth response is relatively rapid

The tissue weights are quantitative

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Custom Animal Models

Price on request

-          Co-development of novel models with a customer, funded by the customer

-          The investment is returned as price reductions when the new model is used by the customer

Even though we keep a constant eye on the... Show more »

-          Co-development of novel models with a customer, funded by the customer

-          The investment is returned as price reductions when the new model is used by the customer

Even though we keep a constant eye on the early drug discovery field and do our best to maintain a broad and ranging service portfolio, you may find that you need a particular model that we do not have. In a case like this, we offer you an option for a co-development project. This is a concept we have used successfully to provide our customers with a fast-track access to novel models.

Those customers who have extensive use for one of our models can also in-license that particular model or assay. Instead of working with us on a project basis, we can transfer the entire model to your laboratory, provide you with all necessary documentation (Standard Operating Procedures, SOPs and Work Instructions, WIs) related to the model, including all future updates, and train your personnel to use the model according to the SOPs and WIs.

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Biology

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Clinical Chemistry

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Pharmacology & Toxicology

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Endocrinology Animal Models

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Cells and Tissues

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Bone/Cartilage Animal Models

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In vitro Disease Models

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Animal Physiology Analyses

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Antibody/Antigen Detection Based Testing

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Clinical Research

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Clinical Laboratory Services

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Safety Pharmacology

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Animal Models of Disease

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Animal Model in vivo Analyses

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Animal Models and Studies

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Specialized Cell-Based Assays

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Metabolic Animal Models

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Lead Identification and Validation

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Assay Development

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Assay Development Consulting

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Custom Cell-Based Assay Development

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Surgical Animal Models

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See "Osteoporosis animal models" and "Osteoarthritis animal models".

See "Osteoporosis animal models" and "Osteoarthritis animal models".

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Drug Target Identification

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Osteoclasts

Price on request

-          Osteoclast differentiation assay

-          Osteoclast activity assay

Pharmatest’s in vitro osteoclast assays can be used for fast demonstration of efficacy of test compounds. We offer studies from... Show more »

-          Osteoclast differentiation assay

-          Osteoclast activity assay

Pharmatest’s in vitro osteoclast assays can be used for fast demonstration of efficacy of test compounds. We offer studies from preliminary testing of a large number of test compounds to extensive testing that will confirm the efficacy and allow calculating EC50 values for the test compounds.

Osteoclast assays are available in human, rat and mouse cells. Human osteoclast cultures are performed using CD34+ human osteoclast precursor cells.

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Cachexia Animal Models

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Cell-Based Assays

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Cell Services

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Breast Xenograft

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Orthotopic and metastasis breast cancer xenograft models using MDA-MB-231(SA), BT-474 and MCF-7 cells.

More information in "Oncology animal models".

Orthotopic and metastasis breast cancer xenograft models using MDA-MB-231(SA), BT-474 and MCF-7 cells.

More information in "Oncology animal models".

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Pancreas Xenograft

Price on request

Orthotopic pancreatic cancer xenografts using BxPC3, Capan-1, Panc-1 and MiaPaCa-2 cells.

More information in "Oncology animal models".

Orthotopic pancreatic cancer xenografts using BxPC3, Capan-1, Panc-1 and MiaPaCa-2 cells.

More information in "Oncology animal models".

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Prostate Xenograft

Price on request

Orthotopic and metastasis prostate cancer xenograft models using LNCaP, 22Rv1, VCaP and PC-3 cells.

More information in "Oncology animal models".

Orthotopic and metastasis prostate cancer xenograft models using LNCaP, 22Rv1, VCaP and PC-3 cells.

More information in "Oncology animal models".

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Cell Invasion Assays

Price on request

Cell invasion is determined using a system where cancer cells are grown on a basement membrane-like MatrigelTM coated filter chamber, and their capacity to migrate through the Matrigel layer is determined.

Cell invasion is determined using a system where cancer cells are grown on a basement membrane-like MatrigelTM coated filter chamber, and their capacity to migrate through the Matrigel layer is determined.

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Xenograft Models

Price on request

-          Breast cancer (with MDA-MB-231(SA), 4T1, BT-474, MCF-7 cells)

-          Prostate cancer (with LNCaP, 22Rv1, VCaP, PC-3 cells)

-          Multiple myeloma (with 5TGM1... Show more »

-          Breast cancer (with MDA-MB-231(SA), 4T1, BT-474, MCF-7 cells)

-          Prostate cancer (with LNCaP, 22Rv1, VCaP, PC-3 cells)

-          Multiple myeloma (with 5TGM1 cells)

-          Pancreatic cancer (with BxPC3, Capan-1, Panc-1, MiaPaCa-2 cells)

In oncology Pharmatest is specialized in orthotopic and metastasis models, and we have special expertise in modeling of bone metastases. The major advantage of these models is the proper tissue microenvironment that is known to affect tumor growth and drug resistance. This increases substantially the clinical relevance and predictivity over traditionally used subcutaneous models that lack the clinically relevant tissue microenvironment.

Pharmatest offers xenograft models in immunodeficient mice, syngeneic models in immunocompetent mice, and xenograft models in humanized mice. Xenograft models include human cancer cell lines that cannot be used in mice that have the normal mouse immune system. Syngeneic models include mouse cell lines that allow using mice with normal immune system, allowing to test immunomodulators. Humanized mice have human immune system, allowing to test immunotherapies targeting human cancer and/or immune cells.

Tumor growth can be monitored by IVIS (when luciferase labelled cancer cells are used) or by serum tumor biomarker measurements. Additionally, in bone metastasis models tumor-induced changes in bone can be measured by X-ray and µCT imaging, and by analysis of serum bone turnover markers. Endpoint measurements can include histology and immunohistochemical stainings.

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Process Consulting

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Human Primary Cells

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Drug Discovery & Development

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In vivo PK/PD Studies

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Drug Discovery

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Animal Model Housing and Maintenance

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Consulting

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Contract Research

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Pharmatest is a CRO specialized in preclinical efficacy services in oncology and skeletal diseases.

Pharmatest is a CRO specialized in preclinical efficacy services in oncology and skeletal diseases.

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Drug Development

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Imaging & Spectroscopy

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Biospecimens

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Nonclinical Research

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Pharmatest is a CRO specialized in preclinical efficacy services in oncology and skeletal diseases.

Pharmatest is a CRO specialized in preclinical efficacy services in oncology and skeletal diseases.

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In vivo Pharmacology Studies

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Preclinical Study Design

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In vivo Drug Efficacy Testing

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Pharmatest is a CRO specialized in preclinical efficacy services in oncology and skeletal diseases.

Pharmatest is a CRO specialized in preclinical efficacy services in oncology and skeletal diseases.

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Contract Services Directory

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Cell Invasion & Migration Assays

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R&D Services

Price on request

-          Co-development of novel models with a customer, funded by the customer

-          The investment is returned as price reductions when the new model is used by the customer

Even though we keep a constant eye on the... Show more »

-          Co-development of novel models with a customer, funded by the customer

-          The investment is returned as price reductions when the new model is used by the customer

Even though we keep a constant eye on the early drug discovery field and do our best to maintain a broad and ranging service portfolio, you may find that you need a particular model that we do not have. In a case like this, we offer you an option for a co-development project. This is a concept we have used successfully to provide our customers with a fast-track access to novel models.

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Medical Imaging Services

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Project Management

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Human Biospecimens

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