Pharmaseed Ltd. brings together 15 years of experience in preclinical translational research and in providing life science companies and research institutes with high quality and reliable preclinical studies in key domains. Our laboratories and facilities are located in multiple locations (France, Israel) and are fully equipped to perform efficacy and safety studies, including pharmacokinetic profiles, acute and chronic evaluation of pharmaceuticals, biological agents and medical devices. Our laboratories in Israel are GLP-certified for toxicology studies.
Our extended network of private and public collaborators all over Europe and the USA allows us to be at the heart of innovation and to propose the latest and most advanced preclinical models and technological tools.
Our expertise in pre-clinical studies lies mostly in the following areas: Stem cell and cellular therapeutics, Cardiovascular diseases, Oncology, Inflammatory and Autoimmune diseases, CNS, Pain, Metabolic Disorders, Toxicology and Medical Devices.
We conduct rodent experiments with CAR-T, pST, MSC and NK cells and adoptive transfer models in oncology and autoimmune diseases. We use IVIS and other means to follow models and diseases progression. We use various readouts using multiplex cytokine panels and more.
animal vaccination and testing for specific immune response. ELISA, FACS, qPCR.
Exhaustion and drug testing with ICPI in vitro. We provide quick service and audited reports.
We do MLR and MLTR, immunotyping and in-vitro drug testing with ICPI and combinations, ELISPOT, exhaustion and more.
Our Expertise and Models for Autoimmune and Inflammatory Diseases
We offer a unique battery of pre-clinical models that allows to screen potential anti-inflammatory agents as well as identify novel therapeutic targets. Screening of compounds can be performed in existing well-characterized animal models as well as in newly developed animal models tailored to a specific tested item.
Our expanded battery of models includes studies of inflammation and inflammatory pathways, including in vivo models of inflammation and autoimmune diseases, as well as complementary in vitro and ex vivo analyses:
Acute and chronic inflammation models:
Neuroinflammatory disease models:
Intestinal Bowel Disease/Colitis models in several species (mouse, rats):
Rheumatoid Arthritis and Osteoarthritis models:
Dermatology models:
Designing better translational models for neurodegenerative diseases
Not Young But Relevant Models
Environmental factors
Behavioral tests
Biochemistry and Histology
Biodistribution studies:
Hemi-crush in rats (moderate spinal cord injury)
Ferric-chloride thrombosis model
Isolation and in vitro assays of rat and murine pancreatic islets.
In multiple species: mice, rats, rabbits, dogs, pigs.
In multiple species: rats, mice, dogs, pigs.
Models for muscular dystrophy (MDX, alpha-sarcoglycan mice).
Evaluation of clinical parameters:
Medical cannabis studies are performed in our laboratories in Israel
• Multiple Sclerosis
• Glaucoma
• ALS
• Peripheral Neuropathic Pain
• Post Operative Pain
• Spinal Cord Injury
• Anxiety – PTSD
• Parkinson’s Disease
• Cachexia, Nausea& Vomiting – Cancer
• Rheumatoid Arthritis
• Inflammatory Bowel Diseases
• Epilepsy
Pharmacology: full analysis capabilities
• Analysis of cannabinoids composition (CBD vs THC); bioinformatic evaluation
• Binding and activity assays CB1/CB2 (cAMP)
• Full panel receptor profile binding
• Basic efficacy in vitro assays
• Animal disease efficacy studies [POC]
• Basic safety (in vivo and in vitro): GLP & non–GLP
Formulation: on-site development and optimization
• Optimization of the bioavailability and delivery standardization, based on the selected clinical indication
• Development of standard carrier (formulation) that will address stability and delivery unmet needs:sublingual, cream/patches, etc.
MOG-induced Experimental Autoimmune Encephalomyelitis (EAE)
Cuprizone-induced model
Behavioral tests (activity box, wheel-running test, social interaction test)
Histological evaluation
DSS-induced colitis in mice and rats
TNBS-induced colitis in mice and rats
T-cell-mediated colitis
Neuropathic pain models:
Our Histology Unit
Our histology unit is aimed at providing expert and cost effective histology and pathology services to our clients, in a complementary or stand-alone service setting. The Histology unit is managed by an expert in-house veterinarian pathologist.
The strength of our histology unit is in our understanding of the experimental design of pre-clinical efficacy or safety studies.
We have extensive experience in the development and implementation of different investigational methodologies utilizing a large array of techniques, including “free floating sections” as a modern method.
We provide researchers with valuable input at the early planning phase of a study, or carry out histology and pathology analysis at any stage of a project, customized to your needs.
Immunohistochemistry and special stains:
Gross pathology:
Cytology
Medical Device Analysis:
Free floating sections: a sectioning method that can be used for immunofluorescence staining. It allows optimal antibody penetration and staining of thick sections that are then imaged through a confocal microscope.
Other services and capabilities:
Behavioural analysis:
Histological (single staining & co-stainings) and bioanalytical evaluation:
Pharmaseed’s regulatory capabilities comprise a vast array of safety and toxicology designs, ranging from acute single-dose studies to chronic and local tolerance studies, including specialty routes such as intra-cerebroventricular administration and infusion. All studies are fully GLP-certified and are in accordance with current guidelines, or custom-designed for particular needs.
Cytokines and cellular markers measurements are done in induced/treated cells, plasma or organs
ELISA and other antibody–mediated assays are available
Safety: Cytotoxicity of Medical device
Cytotoxicity assays are also available for the evaluation of medical devices’ safety.
The medical device is immersed in both polar and non-polar solvents. A live cell culture is exposed to the extract, and the possible toxicity of the medical device is then evaluated using a cytotoxicity assay.
All assays are ISO-10993 compliant.
Pharmaseed offers cytotoxicity / cell viability assays, such as XTT, MTT and LDH kit.
Evaluation of the test item’s effect on proliferation or cell death.
An array of cell lines and/or primary cells is available for the evaluation of the test item.
Determination of EC50/IC50 values – enables the comparison to other cell culture models or materials
Pharmaseed offers cytotoxicity / cell viability assays, such as XTT, MTT and LDH kit.
Evaluation of the test item’s effect on proliferation or cell death.
An array of cell lines and/or primary cells is available for the evaluation of the test item.
Determination of EC50/IC50 values – enables the comparison to other cell culture models or materials
We specialize in Isolation of murine and human stem cells
Differentiation and maturation protocols are available upon request
We provide viability and purity testing, as well as flow cytometry (FACS) analysis for requested markers.
Permanent and transient models of stroke in rats, mice & rabbits
Neuroblastoma, breast cancer, melanoma, pancreatic cancer, colon cancer, multiple myeloma, prostate cancer.
A375 cell line and others
HT29, HCT-116 and other cell lines
MCF7, BT474, MDA-MB-231, T47D and other human cell lines
Hindlimb ischemia models in rats & mice
Lewis Lung Carcinoma Model
LLC cells injected through the tail vein and assessment of lung metastases.
Endothelial Tube Formation
Endothelial Cell Migration & Invasion assays
Murine aortic ring assay
Modified Corneal Pocket Assay in rabbits
In vitro models (determination of NO and hormone levels);
Relaxation test in isolated corpus cavernosum in rabbits
Screening of erectile response in diabetic or hypertension animal models (STZ-induced diabetic rats or SHR)
Wound healing models in normal and diabetic rats and mice
Hindlimb ischemia models in different species (mouse, rat, rabbit):
Stroke models in different species (mouse, rat, rabbit) – embolic and mechanical induction; permanent and transient occlusions; local or distal embolizations:
Ischemic Heart Disease model in different species (mouse, rat) – Transient Left Coronary Artery Occlusion:
Wound Healing model in healthy, diabetic or aged mice
Complementary Angiogenesis models:
Erectile dysfunction models in diabetic, hypertensive or naive rats:
Our Expertise in Oncology and Cancer Models
We offer a wide range of preclinical oncology models and tumor modeling in vivo for the study of cancer growth and metastasis in mice and rats.
The two most used models in oncology R&D for the assessment of potential efficacy of tested technology are the xenograft and the orthotopic model. We combine these in vivo models with in vitro and ex vivo analyses, such as FACS, angiogenesis and cell invasion assays (i.e. HET-CAM assay), and with high-level histological evaluation and imaging tools (Laser Doppler, MRI, X-ray).
Subcutaneous Xenograft Models for a variety of human cancers:
Orthotopic implantation:
Metastatic models (i.e. Lewis Lung Adenocarcinoma systemic administration)
Hollow fiber assay
Complementary models:
Histological evaluation and imaging capabilities
Medical Device Testing Services
Drug Discovery & Development Services
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