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Perfectus Biomed

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Daresbury, GB

About Perfectus Biomed

**Fast-track service available for COVID-19-related research. Click 'Request More Info' button on this page to ask about offer details.**

Perfectus Biomed is a leading UKAS accredited Contract Research Organisation (CRO). We provide both standard and customised microbiology testing services. We have extensive experience with developing ‘fit for purpose’ experiments that mimic ‘real-life’ scenarios. Perfectus Biomed employ a number of PhD microbiologists. Our scientists specialise in antimicrobial, biofilm, viral, fungal and also cellular research. If you would like to find out more about the tests that we can offer then please visit our testing services page. Perfectus Biomed work closely with our clients from a variety of sectors. Sectors include wound care, dental, disinfectants, cosmetics and also water treatment. For a full list of the sectors in which we work, please visit our sectors page. We extend an academically-focused approach to our projects, therefore assigning a PhD scientist to each. This ensures that we fully understand our client’s requirements throughout the entirety of the project.

Our Services (26)


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Microbiology Testing

Price on request

Standard Options:


Perfectus Biomed is a Contract Research Organisation (CRO) that combines an academically-based approach with professionalism, speed and accreditation of industry. We are ISO 9001 certified, offer UKAS accredited ISO 17025 test methods and can perform testing to GLP upon request.


Perfectus Biomed work closely with our clients to understand their requirements. We recognise the difference between generating data for a number of purposes. These include regulatory, marketing and internal purposes.


We offer a wide range of standard test methods, including but not limited to:


EN Standards: 1616, 1276, 1499, 1500, 1650, 1656, 1657, ISO 11135, ISO 11930, 13623, 13624, 13697, 13704, 13726, 13727, 14348, 14349, 14476, 14561, 14562, 14563, 14675, 16437, 16438, 16615, 17111, 17272, 17387


ASTM Standards: D7907, E2149, E2180, E2197, E2315, E2414, E2562, E2647, E2871, F895, G29-96


ISO Standards: 10993, 11737-1, 11930, 14729, 14730, 16954, 18415, 20645, 20743, 22196, 29862


AATCC Test Method 100, AATCC Test Method 127


Custom Options:

Customised test methods are required when:


Standard methods are not quite appropriate for their novel product, test methods are not available and also when internal test methods require independent validation.


Examples of this:


• A novel test product may be in an inappropriate state for the test method e.g. a solid state antimicrobial could not be tested as part of an assay designed for testing liquid antimicrobials.


• An assay designed for testing wound dressings may not account for the variation in dressing layers/structures and absorbencies.


• The unique selling feature of a product may not be demonstrated using standard test methodologies.


• Perfectus Biomed have scientists who understand that there is a commercial requirement for microbiology to go beyond the laboratory bench and to more accurately replicate real life scenarios.


How Perfectus Biomed can help;


• Our services include extensive consultation with clients in order to ensure that we understand the microbiological question you are seeking to answer and the intended data use.


• We tailor our method design so that it is appropriate for the testing of your product.


• All assays include appropriate positive and negative controls and suitable repeats in order to ensure validity of the data.


• We employ PhD microbiologists to run your projects to ensure a detailed understanding of your research.



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Biofilm Testing

Price on request

Perfectus Biomed are specialists in designing, running and interpreting biofilm assays. We offer both standard and customised biofilm testing models.


Understanding the problem of biofilms

Microorganisms such as bacteria, fungi and yeast can either exist as free floating planktonic entities or they can attach to a surface (or one another) to form a microbial biofilm. In nature, 80% of bacteria exist within a biofilm.

When microorganisms live beneath a biofilm, they are significantly less susceptible to antimicrobial agents (and the host’s immune system).

Standard biofilm testing focuses almost exclusively on planktonic bacteria, As such, current standard testing may underestimate the quantity of active agent required to effectively remove a microbial biofilm.


The type of biofilm testing that we offer

Perfectus Biomed will work with you to help you understand the benefits of the different biofilm testing models. We will then choose a test method that is most suitable for your product.

We will test your product using either commercially available biofilm models or adapt/design a biofilm model that suites your requirement.

Please contact us if you do not see the model you are interested in on the list below.


Examples of available test models:

Microtitre plate assay

Calgary biofilm device: Minimum Biofilm Eradication Concentration (MBEC) and Minimum Biofilm Inhibition Concentration (MBIC)

CDC reactor model (ASTM E2871 and ASTM E2562)

Drip flow reactor model (ASTM E2647)

Colony drip flow reactor model

Porcine skin explant drip flow model

Flow cell reactor model

Models are available using static or continuous flow systems


List of UKAS ISO 17025:2005 accredited biofilm test methodologies:

SOP 536: MBEC

SOP 537: CDC reactor model (Staphylococcus aureus and Pseudomonas aeruginosa)

SOP 538: Drip flow reactor model

SOP 555: CDC reactor model (Candida albicans)

SOP 575: Single Tube Method for Determining the Efficacy of Disinfectants against Bacterial Biofilm


Common variations include:

Multiple range of proven biofilm forming pathogens are available for testing

Single and multi-species assays available as requested

Biofilm testing using anaerobic bacteria

Biofilm testing using yeast and fungi



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Viral Assays

Price on request

Virucidal efficacy testing is important for assessing the activity of a variety of products against viruses. Testing can be carried out in a range of models including suspension tests and hard surface carrier tests. These methods can be used to assess a range of products, such as household and industrial disinfectants. Perfectus Biomed also offer viral barrier testing for wound dressings and many other materials.


Standard test methods include:


TCID50 (Median Tissue Culture Infectious Dose) – to verify viral titre

AATCC 100 – Assessment of Antibacterial Finishes on Textile Materials, Modified for Viruses

ASTM E1052 – Standard Test Method for Efficacy of Antimicrobial Agents Against Viruses in Suspension

ASTM E1053 – Standard Test Method to Assess Virucidal Activity of Chemicals Intended for Disinfection of Inanimate, Nonporous Environmental Surfaces

ASTM E2197 – Standard Quantitative Disk Carrier Test Method for Determining the Virucidal Activities of Liquid Chemical Germicides

EN 14476 – Chemical Disinfectants and Antiseptics – Quantitative Suspension Test for the Evaluation of Virucidal Activity in the Medical Area

Pre-Saturated Towelettes Virucidal Effectiveness Test

Viral barrier testing


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Virology Services

Price on request

Perfectus Biomed is a Contract Research Organisation (CRO). We offer both standard and also customised virology research. Perfectus Biomed combine an academically-based approach with professionalism, speed and accreditation of industry. We are ISO 9001 certified and also offer GLP testing upon request.

Perfectus Biomed work closely with our clients to understand their requirements. We recognise the difference between generating data for a number of purposes. These include regulatory, marketing and internal purposes.


Customised Virology Test Methods

Customised test methods are required when:

Standard methods are not quite appropriate for their novel product, test methods are not available and also when internal test methods require independent validation.

Examples of this:

• A novel test product may be in an inappropriate state for the test method e.g. a solid state antiviral could not be tested as part of an assay designed for testing liquid products.

• An assay designed for testing wound dressings may not account for the variation in dressing layers/structures and absorbencies.

• The unique selling feature of a product may not be demonstrated using standard test methodologies.

• Perfectus Biomed have scientists who understand that there is a commercial requirement for virology to go beyond the laboratory bench and to more accurately replicate real life scenarios.

How Perfectus Biomed can help;

• Our services include extensive consultation with clients in order to ensure that we understand the viral question you are seeking to answer and the intended data use.

• We tailor our method design so that it is appropriate for the testing of your product.

• All assays include appropriate positive and negative controls and suitable repeats in order to ensure validity of the data.

• We employ PhD scientists to run your projects to ensure a detailed understanding of your research.



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Cell-Based Assays

Price on request

Perfectus Biomed specialise in cell culture to support the biological evaluation of your product.

We use in vitro wound models and cell models to aid biocompatibility assessments using ISO 10993 guidance. We measure parameters such as cytotoxicity (USP 87), hemocompatibility, irritation, corrosion and proliferation viability. Testing to GLP is available.


We typically offer cell services to support our clients for one of three reasons:

1. Research and Development: product development, screening and pre-clinical data.

2. Biocompatibility and Safety/regulatory Requirements: legal requirements and label claims.

3. Marketing: evidence to support the product on the market by highlighting competitive advantages, differentiations and USPs.


Please see the below list of cell culture assays that Perfectus Biomed can offer. Please contact us if you would like to discuss a test method with us in more detail.

• Biocompatibility – ISO 10993-3: Tests for genotoxicity

• Biocompatibility – ISO 10993-4: Interaction with blood

• Biocompatibility – ISO 10993-5: in vitro cytotoxicity – Direct contact

• Biocompatibility – ISO 10993-5: in vitro cytotoxicity – Indirect contact

• USP 87 – Cytotoxicity

• In vitro scratch wound closure

• Gel contraction assays

• EpiSkin Reconstructed Human Epidermis – Skin Irritation Assay

• EpiSkin Reconstructed Human Epidermis – Skin Corrosion Assay

• Macrophage activation

• Co-culture with monolayer

• MMP concentration assessments

• Acne models

• Phenotypic screening of drugs

• Basic gut epithelial models

• Cancer invasion assays

• Molecular analysis – pathway targeting

• Genotoxicity

• Cytokine measurement

• Growth factor measurement

• Measurements of extracellular matrix components

• Protease measurement

• Colonised skin models

• Standard anti-viral testing

• Cell proliferation/viability

• Collagen production

• Wound closure in 3D skin

• Biofilm-wound models

• Antiviral fogging

• Corneal model (bacterial adhesion, eye irritation)

• Oral models (oral care, bacterial adhesion)

• Vaginal models (feminine hygiene care/bacterial adhesion)

• Diabetic models

• Gut microbiome models

• Biocidal activity again


Human Cell Types:

Human dermal fibroblasts (normal, adult)

Human epidermal keratinocytes (normal, neonatal)

HeLa (epithelial, adenocarcinoma)

H1-HeLa (epithelial, adenocarcinoma, viral susceptibility)

HEK-293 (epithelial, contain adenovirus DNA)




Animal Cell Types:

Vero (epithelial, normal)

L-929 (fibroblast, normal)

RAW 264.7 (macrophage, Abelson murine leukemia virus-induced tumor)

MDCK (epithelial, normal)



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Pathogen Detection Assay Panels

Price on request

Mycoplasma can attain high densities within cell cultures with an absence of visible morphological changes. Undetected contamination by mycoplasma species or other pathogens invalidate work, and so regular sterility testing is critical for continuous cell lines.



Pathogen detection testing is recommended:

  • When creating a master cell bank or working cell bank
  • Before beggining a new series of experiments
  • When the cell line behaves abnormally
  • Every two months that the cell culture is actively growing
  • Prior to publication of experimental data


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Cell Line Authentication

Price on request

Authentication of cell lines and other key biological materials enhances reproducibility of research findings through increased scientific rigor and transparency. Authentication plans are therefore required for many grant applications to ensure authenticity of the cell line throughout multiple timepoints during continuous use in projects.


During a project, cell line authentication is recommended when:

  • A new cell line is established or acquired
  • A new series of experiments have begun
  • Cell lines are routinely passaged
  • Inconsistent cell behaviour or unexpected results are seen
  • Passage number is limited as cell lines mutate over time
  • You are preparing to publish
  • Cell stocks are being frozen to verify purity

Best practice guidelines recommend cell line testing:

  • Prior to submitting a paper for review or beginning a new grant
  • To prevent cross-contamination
  • If cells give erratic and unreproducible results
  • If a change in morphology or growth characteristics are observed
  • When creating a new cell line
  • When receiving cells from a non-accredited laboratory/provider
  • When creating cell stocks for storage


What we offer:

Perfectus Biomed work to the ATCC ASN-0002-2011 standard, with the ability to test at 24 loci using Promega’s Geneprint 24 system to prevent misidentification of closely related cell lines. The 24 loci that will be tested include: TH01, TPOX, vWA, Amelogenin (for gender identification), CSF1PO, D16S539, D7S820, D13S317 and D5S818. These are the nine markers as outlined within ASN-0002-2011. We provide clients with a signed report that covers the basic requirement for grant/journal submissions. Reports will include the electropherogram analysis and interpretation of the Short Tandem Repeat (STR) profile compared to the published standard profile, where required. 


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Microscopy

Price on request

Microscopy images can be a useful tool for marketing materials. Here at Perfectus Biomed, we can offer:

Light Microscopy

Confocal Microscopy with fluorescent staining, such as LIVE/DEAD stain and imaging.

Scanning Electron Microscopy (SEM) images, including adding false colour.



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Medical Device Testing

Price on request

Perfectus Biomed provide a number of both standard and customised medical device test methods. We can help support your research and development into microbial infection control of medical devices. Please click here for more information on our expertise.

Infections relating to medical implants such as catheters, stents and joint prosthesis can result in a number of healthcare problems. These include local and systemic infection, implant replacement, patient pain and discomfort and associated healthcare costs. An improvement in the antimicrobial nature of medical implants therefore helps to improve patient well-being while also minimising healthcare costs.

Please contact Perfectus Biomed to discuss how we can support research and development programmes related to infection control on medical devices.

Medical Device Test Methods We Offer:

Our testing experience includes working with:

  • Catheter tubing
  • Catheter bags
  • Negative pressure wound therapy devices
  • Implantable medical devices
  • Point of care diagnostic devices
  • 510(k) submission

We offer a range of customised test methodologies which include:

  • Biofilm prevention assays
  • Biofilm removal assays
  • Bacterial percentage coverage analysis
  • LIVE/DEAD imaging
  • Confocal microscopy
  • Scanning Electron Microscopy (SEM)
  • Disinfection efficacy analysis

We also offer a range of standard test methodologies including:

  • ASTM D7907: Standard test methods for determination of bactericidal efficacy on the surface of medical examination gloves
  • BS EN 1616: Sterile urethral catheters for single use
  • BS EN ISO 11737-1: Sterilisation of Medical devices – Microbiological Methods – Part 1: Determination of a -population of microorganisms on products (Bioburden)
  • ISO 22196: Measurement of antibacterial activity on plastics and other non-porous surfaces
  • WHTM 01-01: Decontamination of medical devices within acute services


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Cosmetic and Personal Care Product Testing

Price on request

Cosmetic manufacturers must ensure that their products are free from particular quantities and also species of microorganisms. Cosmetic testing therefore assesses the quantities and species of microorganisms present in the product. The presence of such contaminants could affect the quality of a product and also the health of the consumer.

Types of Cosmetic Test Methodologies

The EU cosmetics regulation 1223/2009 requires cosmetic testing. Microbiological specifications therefore need to be provided for cosmetic products that are placed on the EU market. There are a range of appropriate test methodologies:

  • ISO 22717:2015 – designed to detect Pseudomonas aeruginosa. Lower risk products include those which demonstrate low water activity, hydro-alcoholic products and extreme pH values. Method adaptations are possible depending on the required level of detection.
  • ISO 18416:2015 – utilised for the detection of Candida albicans within cosmetic products.
  • ISO 21150:2015 – used for the detection/identification of Escherichia coli. Lower risk products include those which demonstrate low water activity, hydro-alcoholic products and extreme pH values. Method adaptations are possible depending on the required level of detection.
  • ISO 21149:2006 – gives general guidelines for enumeration and detection of mesophilic aerobic bacteria.
  • ISO 16212:2008 – used for the detection and also the enumeration of yeast and mould within cosmetic products.

Our PhD microbiologists are available to discuss method adaptations with you. We can also custom design test methods to meet your requirements.

We offer a range of standard test methodologies including:

  • ISO 11930 – Cosmetics Microbiology: Evaluation of the antimicrobial protection of a cosmetic product
  • ISO 16212 – Cosmetics Microbiology: Enumeration of yeast and mould
  • ISO 18416 – Cosmetics Microbiology: Detection of Candida albicans
  • ISO 21149 – Cosmetics Microbiology: Enumeration and detection of aerobic mesophilic bacteria
  • ISO 21150 – Cosmetics Microbiology: Detection of Escherichia coli
  • ISO 22717 – Cosmetics Microbiology: Detection of Pseudomonas aeruginosa


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Disinfectant Testing

Price on request

Perfectus Biomed offer a range of planktonic and biofilm disinfectant testing methods. Disinfectant testing methods ascertain the activity of antimicrobial agents under specified conditions.

We work with clients to generate evidence in support of products that consistently remove or inactivate pathogens. Our disinfectant testing can be carried out on inanimate surfaces with and also without interfering substances. Our team of PhD microbiologists are available to discuss method adaptations with you. We can also custom design disinfectant testing that meets with your requirement.

The Disinfectant Tests that We Offer

Traditional disinfectant testing focuses on planktonic isolates. They are typically easier to kill and less persistent than multispecies biofilm-encased microorganisms. Perfectus Biomed recognise that, in nature, an estimated 80% of microorganisms exist within polymicrobial biofilms. With this in mind, we design test methodologies that produce meaningful data.

Perfectus Biomed can provide a number of disinfectant tests, including carrier tests, suspension tests, capacity testing, practical and in use testing. We offer these tests in line with standard test methods including; BS EN 1040, BS EN 1276, BS EN 1650, BS, EN 1656, BS EN 1657, BS EN 13727, BS EN 13624, BS EN 13697, BS EN 13704, BS EN 14476, BS EN 14675, BS EN 14561, ASTM E2197-11, ASTM E2414-05.

If you do not see the disinfectant test you are looking for, please call us to discuss your requirements further.

Disinfectant testing supports infection control in both healthcare settings and in the community. Microorganisms spread by direct and indirect contact and can persist on hard surfaces for prolonged periods of time. Disinfectant manufacturers make claims around their antimicrobial activity relating to time of kill, persistence on a surface and residual activity.

Experts at Perfectus Biomed will work with you to design test methods that replicate ‘real life scenarios’ that demonstrate the efficacy of disinfectant products against potential pathogenic microorganisms, including those that exist within a microbial biofilm.

Our customised test methodologies include:

  • EpiDerm™ skin corrosion test model
  • Minimum inhibitory concentration determination
  • Minimum eradication concentration determination
  • Minimum biofilm eradication concentration determination
  • Algae resistance test based on ASTM G29
  • Custom antimicrobial hard surface testing
  • Room fogging studies
  • Zone of inhibition
  • Residual activity assays

Test products include:

  • Household disinfectants
  • Clinical disinfectants
  • Hand sanitisers
  • Hard surface wipes
  • Fogging devices
  • Aerosols
  • Other – please contact us to discuss.

We also offer a range of standard test methodologies including:

  • BS EN 1040: Basic bactericidal activity of chemical disinfectants
  • BS EN 1276: Quantitative suspension test for the evaluation of bactericidal activity of chemical disinfectants and antiseptics used in food, industrial, domestic and institutional areas.
  • BS EN 1650: Quantitative suspension test for evaluation of fungicidal activity of chemical disinfectants and antiseptics used in food, industrial, domestic and institutional areas.
  • BS EN 1656: Quantitative suspension test for the evaluation of bactericidal activity of chemical disinfectants and antiseptics used in the veterinary area.
  • BS EN 1657: Quantitative suspension test for the evaluation of fungicidal or yeasticidal activity of chemical disinfectants and antiseptics used in the veterinary area.
  • BS EN 13727: Quantitative suspension test for the evaluation of bactericidal activity of chemical disinfectants for instruments used in the medical area.
  • BS EN 13624: Quantitative suspension test for the evaluation of fungicidal or yeasticidal activity in the medical area.
  • BS EN 13697: Quantitative non-porous surface test for the evaluation of bactericidal and/or fungicidal activity of chemical disinfectants used in food, industrial, domestic and institutional areas.
  • BS EN 13704: Quantitative suspension test for the evaluation of sporicidal activity of chemical disinfectants used in food, industrial, domestic and institutional areas.
  • BS EN 14476: Viricidal quantitative suspension test for chemical disinfectants and antiseptics used in human medicine.
  • BS EN 14675: Quantitative suspension test for the evaluation of virucidal activity of chemical disinfectants and antiseptics used in the veterinary area.
  • BS EN 14561: Quantitative carrier test for the evaluation of bactericidal activity for instruments used in the medical area.
  • ASTM E2197-11: Standard quantitative disk carrier test method for determining the bactericidal, virucidal, fungicidal, mycobactericidal and sporicidal activities of liquid chemical germicides.
  • ASTM E2414-05: Standard test method for quantitative sporicidal three step method to determine sporicidal efficacy of liquids, liquid sprays, and vapour or gases on contaminated carrier surfaces.


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Product Testing Services

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Product Testing Services


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Biochemistry & Molecular Biology

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Confocal Microscopy

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Cytotoxicity Assays

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Biocompatibility Testing

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Product Development, Testing, and Packaging

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Product Development, Testing, and Packaging Services


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Toxicology

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Toxicology Services


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Pharmacology & Toxicology

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Pharmacology & Toxicology Services


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Drug Discovery & Development

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Drug Discovery & Development Services


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Medical Devices & Diagnostics

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Medical Devices & Diagnostics Services


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Biology

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Biology Services


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Antimicrobial Activity Testing

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Antimicrobial Activity Testing Services


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Bacterial Culture

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Minimal Bactericidal Concentration (MBC) Assay

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Minimum Inhibitory Concentration (MIC) Assay

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