PEPperPRINT GmbH

PEPperCHIP® Peptide Microarrays enable the high resolution epitope mapping of antibodies and sera by translating one or more antigens into overlapping peptides. Incubation of the resulting peptide microarray with an antibody or serum sample as well as suited secondary antibodies gives rise to spot patterns that correlate with the epitope of the given sample.

Due to the very high spot density and the widely unlimited peptide content flexibility, PEPperCHIP® Peptide Microarrays are the ideal and most economic solution to identify antibody and serum epitopes in the highest resolution possible.

Epitope Mapping Specifications:
The PEPperMAP® Epitope Mapping is the standard epitope mapping format based on a single peptide length and the maximum peptide overlap for high resolution epitope data. The Type 1 Epitope Mapping is the recommended solution for monoclonal antibodies and serum samples.

PEPperCHIP® is the most economic platform for antigen and epitope discovery in a single step. PEPperCHIP® Discovery Microarrays allows serum or antibody screening with up to 28,000 different custom or random peptides. PEPperMAP® Antigen and Epitope Discovery also includes all assays, data quantification and evaluation as well as a detailed scientific report with all raw and microarray data.

Specifications:
- a custom PEPperCHIP® Discovery Microarray
- incubation with your antibody or serum sample
- single isotype analysis (e.g. IgG or IgM)
- staining with control and secondary antibodies
- read-out, data evaluation and reporting

Therapeutic antibodies often bind to conformational epitopes, which can hardly be mapped with linear peptides. Constrained cyclic peptides can overcome this limitation by mimicking looped epitope structures. PEPperCHIP® Cyclic Peptide Microarrays combine the advantages of PEPperPRINT's unrivalled peptide sequence flexibility with on-array cyclization.

By using antigen-specific PEPperCHIP® Cyclic Peptide Microarrays with constrained cyclic peptides, our PEPperMAP® Conformational Epitope Mapping enables the characterization of monoclonal antibodies with conformational epitopes in a uniquely high resolution.

High resolution PEPperMAP® Conformational Epitope Mapping is based on an antigen translated into 7, 10 and 13 aa constrained cyclic peptides with maximum peptide overlap. Different peptide lengths are applied to mimic different loop sized and, thus, epitope conformations.

Specifications:
• a custom peptide microarray with your antigen as overlapping peptides
• 7, 10 and 13 aa peptides with 6, 9 and 12 aa peptide-peptide overlap
• HA control peptides
• on-chip peptide thioether cyclization
• incubation with your antibody or serum sample
• staining with control and secondary antibodies
• read-out and quantification of microarray data
• data evaluation and reporting