PEPperPRINT GmbH

Antibodies are among the most important life science tools for therapeutics, basic research and diagnostic tests. However, mono- and polyclonal antibodies are often poorly characterized in terms of epitope and antigen knowledge, specificity and cross-reactivity.
To address these topics, PEPperPRINT developed a number of disruptive solutions for the in-depth analysis of epitopes, antibodies and antibody cross-reactions with all kinds of samples, antibody isotypes and species in the most flexible, comprehensive and economic manner. These solutions cover a range of applications for e.g.

• Linear and conformational epitope mapping with amino acid resolution
• Epitome-wide antibody cross-reactivity analysis of antibodies
• Identification of conserved and variable amino acid positions of an epitope by full positional substitution scans with all 20 main amino acids
• Antigen, epitope and mimotope discovery

Specifications of PEPperMAP® Linear Epitope Mapping (975€)

The PEPperMAP® Linear Epitope Mapping is the standard epitope mapping format based on a single peptide length and the maximum peptide overlap for high resolution epitope data. The PEPperMAP® Linear Epitope Mapping is the recommended solution for monoclonal antibodies and serum samples.

• Custom peptide microarray with your antigen as overlapping peptides
• For up to 1,000 peptides in duplicate
• 15 amino acid linear peptides printed in duplicate
• Max. peptide overlap of 14 amino acids for high-resolution epitope mapping
• All immunoassays with your antibody or serum sample
• Read-out and quantification of microarray data
• Data evaluation and scientific reporting

Specifications of PEPperMAP® Conformational Epitope Mapping (1995€)

High resolution PEPperMAP® Conformational Epitope Mapping is based on an antigen translated into 7, 10 and 13 aa cyclic constrained peptides with max. peptide overlaps of 6, 9 and 12 aa. Different peptide lengths are applied to mimic different loop sized and hence epitope conformations.

• Custom peptide microarray with your antigen as overlapping peptides
• For up to 500 peptides in duplicate
• 7, 10 and 13 amino acid cyclic constrained peptides printed in duplicate
• Max. peptide overlaps of 6, 9 and 12 amino acids for high-resolution epitope mapping
• All immunoassays with your antibody or serum sample
• Read-out and quantification of microarray data
• Data evaluation and scientific reporting

From single antigens translated into overlapping peptides to whole virus proteomes – PEPperCHIP® Custom Peptide Microarrays can be fully tailored in a uniquely cost-effective manner.

Peptide Synthesis: On-chip using Fmoc chemistry and rountine double coupling steps
Microarray Format: Standard slide format (3'' x 1'', 75.4 mm x 25.0 mm x 1.0 mm)
Number of Peptides: From 130 to 5,500 different peptides printed in duplicate
Assays per Microarray: From single arrays to 16 arrays per chip depending on the number of peptides
Array Content: Fully flexible with custom protein-derived overlapping peptides, peptide collections, substitution scans etc.
Available Amino Acids: L- and D-amino acids, citrulline and other amino acids on request
Peptide Length: Rountine synthesis of up to 15mer peptides, longer peptides on request
Control Peptides: Standard HA (YPYDVPDYAG) and Flag (DYKDDDDKAS) tags, other controls on request

PEPperCHIP® Custom Peptide Microarray (895€)

• Custom peptide microarray with up to 5,500 different peptides printed in duplicate
• Covering any custom epitope, peptide or antigen collection
• Linear peptides with 15 amino acids in length
• Single or multiple arrays per microarray depending on array content

PEPperCHIP® Custom Cyclic Peptide Microarray (995€)

• Custom peptide microarray with up to 1,800 different peptides printed in duplicate
• 7, 10 and 13 amino acid cyclic constrained peptides printed in duplicate
• Max. peptide overlaps of 6, 9 and 12 amino acids for high-resolution epitope mapping
• HA control peptides

Optional add-on services or products:

• PEPperMAP® Assay Service (Incubation with your antibody or serum sample / Staining with control and secondary antibodies / Read-out, data evaluation and reporting)
  OR
• PEPperCHIP® Incubation Tray
• PEPperCHIP® anti-HA control antibodies
• PepSlide® Analyzer Software

Beside custom PEPperCHIP® Peptide Microarrays, we also provide PEPperCHIP® Standard Peptide Microarrays with off-the-shelf content for infectious disease, allergen, cancer and autoimmune research.

PEPperCHIP® Standard Peptide Microarrays cover selected epitopes, antigens or whole viral proteomes translated into overlapping peptides, or random peptide libraries for signature discovery.

PEPperCHIP® Peptide Microarrays for Autoimmune Research

• PEPperCHIP® Autoimmune Epitope Microarray
  -4,287 database-derived B-cell epitopes associated with autoimmune diseases including 286 citrullinated peptides
• PEPperCHIP® Cyclic Citrullinated Peptide Microarray (Thioether Cyclization) (995€)
  -337 cyclic citullinated peptides including the corresponding arginine variants
• PEPperCHIP® Lupus Microarray
  -28 selected Lupus antigens, 789 linear B-cell epitopes

PEPperCHIP® Peptide Microarrays for Cancer Research

• PEPperCHIP® Melanoma Antigen Microarray
  -Selected antigens
• PEPperCHIP® Oncovirus Epitope Microarray
  -3,653 linear B-cell epitopes of human oncoviruses from the Immune Epitope Database including 7 citrullinated peptides.
• PEPperCHIP® Tumor Antigen Microarray
  -Selected antigens (Uniprot IDs P16403, P62805, P14635, P40126, Q2XN98, P04626, Q13296, A2JGV3, P24347, Q16385, P07288, P78358, P01106, P56747, P06731, P40967, P19544, P14679, P48431, Q16653, P68133, P30455)

PEPperCHIP® Peptide Microarrays for Allergen Research

• PEPperCHIP® Allergen Epitope Microarray
  -4,022 linear allergen epitopes based on 223 different allergens of 90 organisms (Uniprot IDs: see Material Data Sheet)

PEPperCHIP® Peptide Microarrays for Infectious Disease Research

• PEPperCHIP® Infectious Disease Epitope Microarray
  -3,760 database-derived B-cell epitopes associated with 196 different pathogens
• PEPperCHIP® BK Polyomavirus Antigen Microarray
  -Selected antigens (Uniprot IDs P03082, P14999, P14996, P03094, P03094-2)
• PEPperCHIP® Chikungunya Virus Microarray
  -Full proteome of Chikungunya Virus (CHIKV ESCA) (Uniprot IDs Q8JUX5, Q8JUX6)
• PEPperCHIP® Coronavirus HCoV-EMC/2012 Proteome Microarray
  -Full proteome of human coronavirus HCoV-EMC/2012
• PEPperCHIP® Dengue Virus Type 1 Proteome Microarray
  -Genome polyprotein (Uniprot ID P33478)
• PEPperCHIP® Dengue Virus Type 2 Proteome Microarray
  -Genome polyprotein (Uniprot ID P14340)
• PEPperCHIP® Dengue Virus Type 3 Proteome Microarray
  -Genome polyprotein (Uniprot ID P27915)
• PEPperCHIP® Dengue Virus Type 4 Proteome Microarray
  -Genome polyprotein (Uniprot ID Q2YHF0)
• PEPperCHIP® Epstein-Barr Virus Antigen Microarray
  -Selected Antigens (Uniprot IDs: see Material Data Sheet)
• PEPperCHIP® Foot-and-mouth Disease Virus Proteome Microarray
  -Genome polyprotein (Uniprot ID P03305)
• PEPperCHIP® HBV Proteome Microarray
  -Proteome of Hepatitis B virus (Uniprot IDs: see Material Data Sheet)
• PEPperCHIP® HBV L-Protein Pre-S and S Antigen Microarray
  -Large envelope proteins (pre-S and S domains, genotypes A2-H)
• PEPperCHIP® HCMV Antigen Microarray
  -Selected antigens (Uniprot IDs: see Material Data Sheet)
• PEPperCHIP® HCV Proteome Microarray
  -Genome polyprotein (Uniprot IDs P26664, P26660, Q81495, P0C045)
• PEPperCHIP® HIV-1 (Env) Antigen Microarray
  -Envelope glycoprotein gp160 (Env) (Uniprot ID P04578)
• PEPperCHIP® Influenza Virus H1N1 Proteome Microarray
  -Selected antigens
• PEPperCHIP® Influenza Virus H5N1 Proteome Microarray
  -Selected antigens
• PEPperCHIP® Japanese Encephalitis Virus Proteome Microarray
  -Genome polyprotein (Uniprot ID P27395)
• PEPperCHIP® Mycobacterium Tuberculosis Antigen Microarray
  -Selected antigens
• PEPperCHIP® SARS-CoV Antigen Microarray
  -Selected antigens (Uniprot IDs P59594, P59595, Q19QW6, P59637)
• PEPperCHIP® Tetanus Toxin Microarray
  -Tatanus toxin (Uniprot ID P04958)
• PEPperCHIP® Tick-borne Encephalitis Virus Proteome Microarray
  -Genome polyprotein (Uniprot ID P14336)
• PEPperCHIP® Trypanosoma Brucei Antigen Microarray
  -Selected antigens (Genbank IDs AJ304413.1, HQ662603.1, Uniprot ID P02896)
• PEPperCHIP® Vaccinia Virus Antigen Microarray
  -Selected antigens (Uniprot IDs O57265, O57206, O57196, O57223, P68598, O57211)
• PEPperCHIP® West Nile Virus Proteome Microarray
  -Genome polyprotein (Uniprot ID P06935)
• PEPperCHIP® Yellow Fever Virus Proteome Microarray
  -Genome polyprotein (Uniprot IDs P00314, Q6DV88)
• PEPperCHIP® Zika Virus Proteome Microarray (475€)
  -Zika virus genome polyprotein (Uniprot ID Q32ZE1)

PEPperCHIP® Peptide Microarrays for Signature Discovery

• PEPperCHIP® Signature Microarray
  -Random peptides (5,500 peptides printed in duplicate)

Antibodies are among the most important life science tools for therapeutics, basic research and diagnostic tests. However, mono- and polyclonal antibodies are often poorly characterized in terms of epitope and antigen knowledge, specificity and cross-reactivity.

In order to address these topics, PEPperPRINT developed a number of disruptive solutions for the in-depth analysis of epitopes, antibodies and antibody cross-reactions with all kinds of samples, antibody isotypes and species in the most flexible, comprehensive and economic manner. These solutions cover a range of applications for e.g.

• Linear and conformational epitope mapping with amino acid resolution
• Epitome-wide antibody cross-reactivity analysis of antibodies
• Identification of conserved and variable amino acid positions of an epitope by full positional substitution scans with all 20 main amino acids
• Antigen, epitope and mimotope discovery

Specifications of PEPperMAP® Linear Epitope Mapping (975€)

The PEPperMAP® Linear Epitope Mapping is the standard epitope mapping format based on a single peptide length and the maximum peptide overlap for high resolution epitope data. The PEPperMAP® Linear Epitope Mapping is the recommended solution for monoclonal antibodies and serum samples.

Specifications of PEPperMAP® Conformational Epitope Mapping (1995€)

High resolution PEPperMAP® Conformational Epitope Mapping is based on an antigen translated into 7, 10 and 13 aa cyclic constrained peptides with max. peptide overlaps of 6, 9 and 12 aa. Different peptide lengths are applied to mimic different loop sized and hence epitope conformations.

Specifications of PEPperMAP® Antibody Cross-Reactivity Analysis (2695€)

Epitome-wide profiling of antibody cross-reactions including a bioinformatic analysis of common motifs and antibody specificity

Specifications of PEPperMAP® Epitope Substitution Scan (1395€)

Epitope Single Substitution Scans cover the stepwise substitution of all amino acid positions of a given epitope or peptide with all 20 main amino acids and optionally additional non-natural amino acids.

PEPperPRINT provides synthetic peptides for various applications like ELISA tests, competition assays, antibody generation, immunizations, the characterization of protein interaction sites, spotting and affinity purification.

Custom Peptide Synthesis - Specifications:

• Peptide length: up to 60 amino acid peptides
• Amount: from 1 mg small scale for proteomics up to 5 g
• Purity: crude peptides, ≥70%, ≥90%, ≥95% and ≥98%
• Including mass spectrometry documentation
• Including HPLC documentation for purified peptides

The identification of early biomarkers for severe diseases by noninvasive methods is very important but demanding. In this regard high-content peptide microarrays provide a very efficient tool for antibody biomarker discovery from sera. The flexibility of the PEPperCHIP® platform allows the step-wise screening from e.g. 35,000 peptides for hit discovery down to handful peptides for hit validation.

Specifications

Array content: PEPperCHIP®Custom /Standard Peptide Microarray or PEPperCHIP® Discovery Microarray with random, pre-selected or overlapping peptides

Input Data & Sample Material

• Antigen sequences (e.g. FASTA format or UniProt ID) or peptide library (Excel file)
• PEPperCHIP® Custom /Standard Peptide Microarray: 20 µl serum or 20 µg purified antibody of each patient or patient pool
• PEPperCHIP® Discovery Arrays: 100 µl serum or 100 µg purified antibody of each patient or patient pool

Analysis

PEPperCHIP® analysis is straightforward and can be performed in your own lab or covered by our PEPperMAP® Service.

The identification of early biomarkers for severe diseases by noninvasive methods is very important but demanding. In this regard high-content peptide microarrays provide a very efficient tool for antibody biomarker discovery from sera. The flexibility of the PEPperCHIP® platform allows the step-wise screening from e.g. 35.000 peptides for hit discovery down to handful peptides for hit validation.

Specifications

Array content: PEPperCHIP®Custom /Standard Peptide Microarray or PEPperCHIP® Discovery Microarray with random, pre-selected or overlapping peptides

Input Data & Sample Material

• Antigen sequences (e.g. FASTA format or UniProt ID) or peptide library (Excel file)
• PEPperCHIP® Custom /Standard Peptide Microarray: 20 µl serum or 20 µg purified antibody of each patient or patient pool
• PEPperCHIP® Discovery Arrays: 100 µl serum or 100 µg purified antibody of each patient or patient pool

Analysis

PEPperCHIP® analysis is straightforward and can be performed in your own lab or covered by our PEPperMAP® Service.