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PEPperPRINT GmbH

Heidelberg, DE

PEPperPRINT is a young and innovative biotech company based in Heidelberg, Germany, and a spin-off of the German Cancer Research Center. We provide the new peptide microarray platform PEPperCHIP® for:

  • Antigen and Epitope Discovery
  • Epitope Mapping
  • Epitope Substitution Scans
  • Immune Monitoring of Anti-Drug Antibodies
  • Vaccine and Adjuvant Development
  • Complicance Marker Discovery and Development
  • Serum Biomarker Discovery
  • Peptide Target Binder Discovery and Development

With our proprietary peptide laser printing technology, the high-density PEPperCHIP® Microarrays set a new standard in terms of peptide diversity, signal quality and content flexibility.

We invite you to visit our at www.pepperprint.com to get an insight into our products & services, the related background technologies, or PEPperPRINT's company history. For more information about possible applcations, proposals and... Show more »

PEPperPRINT is a young and innovative biotech company based in Heidelberg, Germany, and a spin-off of the German Cancer Research Center. We provide the new peptide microarray platform PEPperCHIP® for:

  • Antigen and Epitope Discovery
  • Epitope Mapping
  • Epitope Substitution Scans
  • Immune Monitoring of Anti-Drug Antibodies
  • Vaccine and Adjuvant Development
  • Complicance Marker Discovery and Development
  • Serum Biomarker Discovery
  • Peptide Target Binder Discovery and Development

With our proprietary peptide laser printing technology, the high-density PEPperCHIP® Microarrays set a new standard in terms of peptide diversity, signal quality and content flexibility.

We invite you to visit our at www.pepperprint.com to get an insight into our products & services, the related background technologies, or PEPperPRINT's company history. For more information about possible applcations, proposals and thorough suppprt, please don't hesitate to contact us at pepperchip@pepperprint.com.

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PEPperPRINT GmbH has not listed any services.

Epitope Mapping
Starting at $945.00 per sample

PEPperCHIP® Peptide Microarrays enable the high resolution epitope mapping of antibodies and sera by translating one or more antigens into overlapping peptides. Incubation of the resulting peptide microarray with an antibody or serum sample as well as suited secondary antibodies gives rise to spot patterns that correlate with the... Show more »

PEPperCHIP® Peptide Microarrays enable the high resolution epitope mapping of antibodies and sera by translating one or more antigens into overlapping peptides. Incubation of the resulting peptide microarray with an antibody or serum sample as well as suited secondary antibodies gives rise to spot patterns that correlate with the epitope of the given sample.

Due to the very high spot density and the widely unlimited peptide content flexibility, PEPperCHIP® Peptide Microarrays are the ideal and most economic solution to identify antibody and serum epitopes in the highest resolution possible.

Epitope Mapping Specifications:
The PEPperMAP® Epitope Mapping is the standard epitope mapping format based on a single peptide length and the maximum peptide overlap for high resolution epitope data. The Type 1 Epitope Mapping is the recommended solution for monoclonal antibodies and serum samples.

PEPperCHIP® is the most economic platform for antigen and epitope discovery in a single step. PEPperCHIP® Discovery Microarrays allows serum or antibody screening with up to 28,000 different custom or random peptides. PEPperMAP® Antigen and Epitope Discovery also includes all assays, data quantification and evaluation as well as a detailed scientific report with all raw and microarray data.

Specifications:
- a custom PEPperCHIP® Discovery Microarray
- incubation with your antibody or serum sample
- single isotype analysis (e.g. IgG or IgM)
- staining with control and secondary antibodies
- read-out, data evaluation and reporting

Therapeutic antibodies often bind to conformational epitopes, which can hardly be mapped with linear peptides. Constrained cyclic peptides can overcome this limitation by mimicking looped epitope structures. PEPperCHIP® Cyclic Peptide Microarrays combine the advantages of PEPperPRINT's unrivalled peptide sequence flexibility with on-array cyclization.

By using antigen-specific PEPperCHIP® Cyclic Peptide Microarrays with constrained cyclic peptides, our PEPperMAP® Conformational Epitope Mapping enables the characterization of monoclonal antibodies with conformational epitopes in a uniquely high resolution.

High resolution PEPperMAP® Conformational Epitope Mapping is based on an antigen translated into 7, 10 and 13 aa constrained cyclic peptides with maximum peptide overlap. Different peptide lengths are applied to mimic different loop sized and, thus, epitope conformations.

Specifications:
• a custom peptide microarray with your antigen as overlapping peptides
• 7, 10 and 13 aa peptides with 6, 9 and 12 aa peptide-peptide overlap
• HA control peptides
• on-chip peptide thioether cyclization
• incubation with your antibody or serum sample
• staining with control and secondary antibodies
• read-out and quantification of microarray data
• data evaluation and reporting

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Peptide Microarray
Starting at $895.00 per chip

From single antigens translated into overlapping peptides to whole virus proteomes – PEPperCHIP® Custom Peptide Microarrays can be fully tailored in a uniquely cost-effective manner.

Specifications:
Peptide Synthesis: On-chip using Fmoc chemistry and rountine double coupling steps
Microarray Format: Standard slide format... Show more »

From single antigens translated into overlapping peptides to whole virus proteomes – PEPperCHIP® Custom Peptide Microarrays can be fully tailored in a uniquely cost-effective manner.

Specifications:
Peptide Synthesis: On-chip using Fmoc chemistry and rountine double coupling steps
Microarray Format: Standard slide format (3'' x 1'', 75.4 mm x 25.0 mm x 1.0 mm)
Number of Peptides: From 100 to 4,400 different peptides printed in duplicate
Assays per Microarray: From single arrays to 16 arrays per chip depending on the number of peptides
Array Content: Fully flexible with custom protein-derived overlapping peptides, peptide collections, substitution scans etc.
Available Amino Acids:L- and D-amino acids, citrulline and other amino acids on request
Peptide Length: Rountine synthesis of up to 15mer peptides, longer peptides on request
Control Peptides:Standard HA (YPYDVPDYAG) and Flag (DYKDDDDKAS) tags, other controls on request

We support you with your optimal array design and project setup. Please contact us at pepperchip@pepperprint.com to discuss your research project.

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Peptide Synthesis
Price on request

Custom peptidesPEPperPRINT's product portfolio is complemented by synthetic peptides for ELISA tests, competition assays, immunizations, spotting, affinity purification and various other applications. Our peptide labs routinely cover the following peptide specifications:

  • raw and HPLC purified custom peptides (95% purity... Show more »

Custom peptidesPEPperPRINT's product portfolio is complemented by synthetic peptides for ELISA tests, competition assays, immunizations, spotting, affinity purification and various other applications. Our peptide labs routinely cover the following peptide specifications:

  • raw and HPLC purified custom peptides (95% purity on demand)
  • 1 mg, 5 mg and 10 mg batches
  • various modifications available (phosphorylation, PEGylation, biotinylation, acetylation, methylation, dye labeling etc.)
  • linear and cyclic peptides

Peptide orders are supplemented by mass spectra as well as HPLC-data in case of purified peptides. HPLC-purified peptides are available from 12 € per amino acid, raw peptides from 8 € per amino acid. This is exemplified by the following calculations:

  • ca. 1 mg of a 15 aa HPLC-purified peptide is available for 185 €
  • ca. 1 mg of a 15 aa HPLC-purified peptide including N-terminal biotinylation is available for 220 €
  • 5 mg of a 20 aa raw peptide with PEGylation are available for 350 €

Please don't hesitate to ask for more information or for a quote according to your specifications. We look forward to provide you with any support and information needed to advance your research with optimal peptide solutions. For more information, please also visit http://www.pepperprint.com/products/custom-peptides/.

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peptide
Biomarker Discovery
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Antibody Screening
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Therapeutic Monoclonal Antibody (mAb) Development
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Proteomics
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Chemistry and Materials
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Synthetic Chemistry
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Biomarkers
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Antibody Services
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Protein Services
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Protein Array
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Omics
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Pharmacology & Toxicology
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Biology
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Bioanalysis
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Biochemistry & Molecular Biology
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