Penn Genomic Analysis Core
9.9/10 Overall Rating
100% Positive
16 Orders Completed
Philadelphia, Pennsylvania, US
The DNA Sequencing Facility is an Abramson Cancer Center Shared Resource that is approved and partially funded by the National Cancer Institute.
Whole exome sequencing on Ion Torrent Proton sequencer. Up to 80 million reads and 12Gb throughput. Results in 1 - 3 weeks.
16S rRNA sequencing on Ion Torrent PGM sequencer. Scalable throughput from 500k to 5m reads, and 100Mb to 1Gb. Results in 1-3 weeks.
Microsatellite-based Genotyping/Fragment Sizing on ABI 3130. Also available are: full service genotyping (including PCR, pooling, sizing and genotyping), LOH Analysis, SNaPshot, and mouse genotyping (for transgene identification). Please contact us for options and information.
High throughput sequencing on Ion Torrent Proton and Personal Genome Machine instruments.
Proton sequencing: Up to 80m reads and 12Gb. Read lengths up to 200bp.
PGM sequencing: Scalable throughput from 500k to 5m reads and 100Mb to 1Gb per run. Read lengths up to 400bp.
AmpliSeq, 16S, and fragment libraries
Microsatellite-based Genotyping/Fragment Sizing on ABI 3130. Also available are: full service genotyping (including PCR, pooling, sizing and genotyping), LOH Analysis, SNaPshot, and mouse genotyping (for transgene identification). Please contact us for options and information.
Bioinformatic analysis including DNA-Seq variant calling, RNA-Seq differential expression, microarray analysis.
PCR amplification, with optional purification via enzymatic cleanup (ExoSAP), spin column, SPRI bead, gel, or Pippinprep.
Purification of PCR amplicon via enzymatic cleanup (ExoSAP), spin column, SPRI bead, gel, or Pippinprep.
Plasmid Miniprep, Midiprep, or Maxiprep.
Low throughput (<= 48 samples), high throughput (> 48 samples), and ready to load samples.
Site-directed Mutation
Plasmid subcloning and BAC recombineering. Targeting Vector Construction for gene targeting experiments including
conditional knock-out construct and knock-in construct.
"Penn Genomic Analysis Core was quick to respond to questions, easy to work with, and extremely fast in delivering the results (less than a day after sample submission). I would definitely recommend and will be using their services again!"
"Good communication and careful workers."
"Timely, responsive, quality data and people!"
"Good communication, willing to troubleshoot and see the project to completion. Thank you."
"Great communication, very helpful, and returned just what I wanted. Thanks"
"very professional, good quality data and rapid completion of project. highly recommended."
Penn Genomic Analysis Core has not received any endorsements.