1352601184186 7fffb663120dd173020ed4b9bc223583

Penn Genomic Analysis Core

Philadelphia, Pennsylvania, US

The DNA Sequencing Facility is an Abramson Cancer Center Shared Resource that is approved and partially funded by the National Cancer Institute.

Penn Genomic Analysis Core has not listed any services.

Recombineering
Recombination-Mediated Genetic Engineering
Price on request
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DNA Sequencing Data Analysis
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Whole Exome Sequencing (WES)
Price on request

Whole exome sequencing on Ion Torrent Proton sequencer. Up to 80 million reads and 12Gb throughput. Results in 1 - 3 weeks.

Whole exome sequencing on Ion Torrent Proton sequencer. Up to 80 million reads and 12Gb throughput. Results in 1 - 3 weeks.

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16S rRNA Sequencing
Price on request

16S rRNA sequencing on Ion Torrent PGM sequencer. Scalable throughput from 500k to 5m reads, and 100Mb to 1Gb. Results in 1-3 weeks.

16S rRNA sequencing on Ion Torrent PGM sequencer. Scalable throughput from 500k to 5m reads, and 100Mb to 1Gb. Results in 1-3 weeks.

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Small RNA Sequencing
Price on request

Small RNA sequencing on Ion Torrent Proton or PGM. Scalable throughput from 500k to 80m reads and 100Mb to 12Gb. Results in 1-3 weeks.

Small RNA sequencing on Ion Torrent Proton or PGM. Scalable throughput from 500k to 80m reads and 100Mb to 12Gb. Results in 1-3 weeks.

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RNA Sequencing
Price on request

Whole transcriptome, targeted transcriptome, and custom targeted RNA sequencing on Ion Torrent Proton or PGM. Scalable throughput from 500k to 80m reads and 100Mb to 12Gb. Results in 1-3 weeks.

Whole transcriptome, targeted transcriptome, and custom targeted RNA sequencing on Ion Torrent Proton or PGM. Scalable throughput from 500k to 80m reads and 100Mb to 12Gb. Results in 1-3 weeks.

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Transfection
Price on request

Contact for detailed pricing information.

Contact for detailed pricing information.

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Genotyping Services
Price on request

Microsatellite-based Genotyping/Fragment Sizing on ABI 3130. Also available are: full service genotyping (including PCR, pooling, sizing and genotyping), LOH Analysis, SNaPshot, and mouse genotyping (for transgene identification). Please contact us for options and information.

Microsatellite-based Genotyping/Fragment Sizing on ABI 3130. Also available are: full service genotyping (including PCR, pooling, sizing and genotyping), LOH Analysis, SNaPshot, and mouse genotyping (for transgene identification). Please contact us for options and information.

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Ion Torrent Sequencing
Ion Torrent Next Generation Sequencing
Price on request

High throughput sequencing on Ion Torrent Proton and Personal Genome Machine instruments.

Proton sequencing: Up to 80m reads and 12Gb. Read lengths up to 200bp.

PGM sequencing: Scalable throughput from 500k to 5m reads and 100Mb to 1Gb per run. Read lengths up to 400bp.

High throughput sequencing on Ion Torrent Proton and Personal Genome Machine instruments.

Proton sequencing: Up to 80m reads and 12Gb. Read lengths up to 200bp.

PGM sequencing: Scalable throughput from 500k to 5m reads and 100Mb to 1Gb per run. Read lengths up to 400bp.

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Ion Torrent NGS Library Preparation
Price on request

AmpliSeq, RNA-seq, 16S, and fragment libraries

AmpliSeq, RNA-seq, 16S, and fragment libraries

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Microsatellite Instability Testing
Price on request

Microsatellite-based Genotyping/Fragment Sizing on ABI 3130. Also available are: full service genotyping (including PCR, pooling, sizing and genotyping), LOH Analysis, SNaPshot, and mouse genotyping (for transgene identification). Please contact us for options and information.

Microsatellite-based Genotyping/Fragment Sizing on ABI 3130. Also available are: full service genotyping (including PCR, pooling, sizing and genotyping), LOH Analysis, SNaPshot, and mouse genotyping (for transgene identification). Please contact us for options and information.

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NGS Data Analysis
Next generation sequencing data analysis
Price on request

Bioinformatic analysis including DNA-Seq variant calling, RNA-Seq differential expression, microarray analysis.

Bioinformatic analysis including DNA-Seq variant calling, RNA-Seq differential expression, microarray analysis.

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PCR
Price on request

PCR amplification, with optional purification via enzymatic cleanup (ExoSAP), spin column, SPRI bead, gel, or Pippinprep.

PCR amplification, with optional purification via enzymatic cleanup (ExoSAP), spin column, SPRI bead, gel, or Pippinprep.

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PCR Product Purification
Price on request

Purification of PCR amplicon via enzymatic cleanup (ExoSAP), spin column, SPRI bead, gel, or Pippinprep.

Purification of PCR amplicon via enzymatic cleanup (ExoSAP), spin column, SPRI bead, gel, or Pippinprep.

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Plasmid DNA Preparation
Price on request

Plasmid Miniprep, Midiprep, or Maxiprep.

Plasmid Miniprep, Midiprep, or Maxiprep.

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Sanger Sequencing
Price on request

Low throughput (<= 48 samples), high throughput (> 48 samples), and ready to load samples.

Low throughput (<= 48 samples), high throughput (> 48 samples), and ready to load samples.

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Site-Directed Mutagenesis
Price on request

Site-directed Mutation

Site-directed Mutation

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Molecular Cloning
Price on request

Plasmid subcloning and BAC recombineering. Targeting Vector Construction for gene targeting experiments including
conditional knock-out construct and knock-in construct.

Plasmid subcloning and BAC recombineering. Targeting Vector Construction for gene targeting experiments including
conditional knock-out construct and knock-in construct.

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DNA Sequencing
Price on request
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Sequencing Data Analysis
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Cell and Tissue Culture
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RNA Services
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Nucleic Acid Analysis
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Exome Sequencing Services
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Nucleic Acid Services
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DNA Services
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Nucleic Acid Data Analysis
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Targeted Gene Sequencing
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Next Generation Sequencing (NGS)
Price on request
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Mutagenesis
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Biochemistry & Molecular Biology
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DNA Library Preparation
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Cells and Tissues
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Plasmids and Vectors
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Genetic Engineering
Price on request

Genetic Engineering Services

Genetic Engineering Services

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2018-10-23 08:19:41 -0700

Net Promoter Score of 10 received for Nucleic Acid Services.

Additional Ratings: satisfaction with deliverable: 10, satisfaction with timeliness: 10.
September 20, 2018

Positive review received for RNA Services:

"Penn Genomic Analysis Core was quick to respond to questions, easy to work with, and extremely fast in delivering the results (less than a day after sample submission). I would definitely recommend and will be using their services again!"

2018-09-18 06:31:35 -0700

Net Promoter Score of 10 received for RNA Services.

Additional Ratings: satisfaction with deliverable: 10, satisfaction with timeliness: 10.
May 9, 2018

Positive review received for Microsatellite Instability Testing:

"Good communication and careful workers."

2018-05-01 08:29:08 -0700

Net Promoter Score of 9 received for Microsatellite Instability Testing.

Additional Ratings: satisfaction with deliverable: 9, satisfaction with timeliness: 9.
2017-12-22 08:11:33 -0800

Net Promoter Score of 10 received for Sanger Sequencing.

Additional Ratings: satisfaction with deliverable: 10, satisfaction with timeliness: 10.
August 18, 2017

Positive review received for Sanger Sequencing:

"Timely, responsive, quality data and people!"

July 21, 2017

Positive review received for Microsatellite Instability Testing:

"Good communication, willing to troubleshoot and see the project to completion. Thank you."

May 28, 2017

Positive review received for Site-Directed Mutagenesis:

"Great communication, very helpful, and returned just what I wanted. Thanks"

2016-06-20 17:12:20 -0700

Net Promoter Score of 10 received for PCR.

Additional Ratings: satisfaction with deliverable: 10, satisfaction with timeliness: 8.
October 18, 2012

Positive review received for Ion Torrent Sequencing:

"very professional, good quality data and rapid completion of project. highly recommended."

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