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Pelvipharm SAS

Founded in 1999, PELVIPHARM is a spin-off from the University of Versailles-Saint-Quentin-en-Yvelines (France) relying on multi-disciplinary talents (medical doctor, pharmaco-economist, veterinarian, pharmacists and PhDs) who deliver both high quality academic standards and reliable contract research services :

  • Preclinical studies based on validated efficacy models including delivery of protocol development, study conduct, report, communication and peer-reviewed publication
  • Guidance for selection of new chemical entities including testing with targeted assays according to potential mechanisms of action
  • Identification of new properties or attributes supporting differentiation of medications within the class or between classes
  • Advice in clinical development for new therapeutic indications or enhancements to address unmet medical need or significant commercial opportunities

**Selected... Show more »

Founded in 1999, PELVIPHARM is a spin-off from the University of Versailles-Saint-Quentin-en-Yvelines (France) relying on multi-disciplinary talents (medical doctor, pharmaco-economist, veterinarian, pharmacists and PhDs) who deliver both high quality academic standards and reliable contract research services :

  • Preclinical studies based on validated efficacy models including delivery of protocol development, study conduct, report, communication and peer-reviewed publication
  • Guidance for selection of new chemical entities including testing with targeted assays according to potential mechanisms of action
  • Identification of new properties or attributes supporting differentiation of medications within the class or between classes
  • Advice in clinical development for new therapeutic indications or enhancements to address unmet medical need or significant commercial opportunities

Selected Publications

  • ASSALY-KADDOUM R, GIULIANO F, LAURIN M, GORNY D, KERGOAT M, BERNABE J, VARDI Y, ALEXANDRE L, BEHR-ROUSSEL D. Low Intensity Extracorporeal Shockwave Therapy (Li-ESWT) Improves Erectile Function in a model of Type II Diabetes Independently of NO/cGMP Pathway. J. Urol. [Epub ahead of print] - 2016 : doi: 10.1016/j.juro.2016.03.147
  • FACCHINETTI P, GIULIANO F, LAURIN M, BERNABE J, CLEMENT P. Direct brain projections onto the spinal generator of ejaculation in the rat. Neuroscience - 2014 : 272C:207–216
  • GELEZ H, GREGGAIN-MOHR J, PFAUS JG, ALLERS KA, GIULIANO F. Flibanserin treatment increases appetitive sexual motivation in the female rat. J Sex Med. - 2013 : May;10(5):1231-9
  • GELEZ H, CLEMENT P, COMPAGNIE S, GORNY D, LAURIN M, ALLERS K, SOMMER B, GIULIANO F. Brain neuronal activation induced by flibanserin treatment in female rats. Psychopharmacology (Berl) - 2013 : 230(4):639-52
  • CLEMENT P, BERNABE J, COMPAGNIE S, ALEXANDRE L, MCCALLUM S, GIULIANO F. Inhibition of ejaculation by the non-peptide oxytocin receptor antagonist GSK557296: a multi-level site of action. Br J Pharmacol - 2013 : 169(7):1477-85
  • PHE V, BEHR-ROUSSEL D, OGER-ROUSSEL S, ROUPRET M, CHARTIER-KASTLER E, LEBRET T, KARSENTY G, COMPERAT E, CAMPARO P, GIULIANO F. Involvement of connexins 43 and 45 in functional mechanism of human detrusor overactivity in neurogenic bladder. Urology - 2013 : 2013 May;81(5):1108.e1-6
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Animal Plasma and Serum
Price on request
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Functional Human Tissue Assays
Price on request

OBJECTIVES

Direct investigation of human tissue physiology and pharmacology, thus very relevant in terms of "transposition" to human pathologies.
Evaluation of ex vivo contraction and relaxation processes of various smooth muscles.
Unique opportunity to evaluate the effect of a drug and its mechanism of action on... Show more »

OBJECTIVES

Direct investigation of human tissue physiology and pharmacology, thus very relevant in terms of "transposition" to human pathologies.
Evaluation of ex vivo contraction and relaxation processes of various smooth muscles.
Unique opportunity to evaluate the effect of a drug and its mechanism of action on human physiological functions at the preclinical stage of development.
In vitro evaluation of the effect of drugs at modulating smooth muscle tone in normal or pathological conditions.
Determination and comparison of potency (EC50), efficiency (Emax) and tissue selectivity of drugs by performing concentration response curves.
Determination of drug affinity (pA2) for a specific receptor and a specific tissue.
HUMAN TISSUE AVAILABLE

Bladder from control patients (with no known bladder dysfunction according to their medical chart).
Bladder from neurogenic patients
Prostate from control patients
Prostate from patients with benign prostatic hyperplasia
Corpus cavernosum
Seminal vesicles
Other tissues can be obtained upon specific request

SOURCE OF HUMAN TISSUES SAMPLE

Pelvipharm collaborates with public and private hospitals located in Paris and other regions all over France.
The collection and use of tissue is carried out in accordance with the Research Plan, all relevant laws, regulations and codes of practice (including obtaining informed consent of patients in writing and determination of hepatitis and HIV serologies).
Human tissues are transported to Pelvipharm facilities via a specialized carrier ensuring security and adapted condition of storage
HUMAN TISSUE PREPARATION

Sections are excised from the sample of each donor for each experiment.
Tissue strips are mounted isometrically to force transducers in organ bath filled with Krebs-HEPES buffer (37°C; 95% O2 and 5% CO2).
Following amplification, the tension changes are digitalized via a Mac Lab TM/8 using Chart TM 5 software (AD Instruments Ltd, Australia).
Appropriate pharmacological agents and/or electrical field stimulation are then applied on the tissue strips after adequate priming of the tissue.

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Erectile Dysfunction Animal Models
Price on request

OBJECTIVES

Electrical stimulation of the cavernous nerve (ES CN) in anesthetized animals aims at evaluating the extent of an erectile dysfunction but also the beneficial pro-erectile facilitator effect of a substance administered either chronically or acutely.

SUMMARIZED METHODOLOGY

Erectile responses are elicited by ES... Show more »

OBJECTIVES

Electrical stimulation of the cavernous nerve (ES CN) in anesthetized animals aims at evaluating the extent of an erectile dysfunction but also the beneficial pro-erectile facilitator effect of a substance administered either chronically or acutely.

SUMMARIZED METHODOLOGY

Erectile responses are elicited by ES CN and measured by monitoring intracavernous pressure (ICP) in anesthetized animals. The carotid artery and corpus cavernosum are catheterized to record blood pressure (BP) and ICP respectively. A bipolar platinum electrode connected to an electrical stimulator is place on the CN to allow ES at different stimulation frequencies in view of establishing a frequency-response curve.

ENDPOINTS

Mean arterial pressure (MAP)
ΔICP (mmHg) / MAP (mmHg) x 100 with ΔICP being the difference between ICP (intracavernosal pressure in the flaccid state, i.e. before stimulation and ICP during the plateau phase of the erectile response,
AUC / MAP with AUC, the area under the curve during the erectile response, measured from the beginning of the electrical stimulation until the end of the erectile response and determined using the ICP level in the flaccid state before the onset of the stimulation.
ICP increase and AUC are normalized with MAP to account for the close influence of the systemic blood pressure in the amplitude of ICP increase during the plateau phase of the erectile response.

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Tissue/Organ Bath Preparation
Price on request

ANIMAL TISSUES

In vitro functional investigation of animal tissue function in normal and pathophysiological conditions.
Unrestricted amount of tissue.
Evaluation of the ability of drugs at modulating different smooth muscle tone can be performed on contractile or relaxant response elicited by pharmacological agents or by... Show more »

ANIMAL TISSUES

In vitro functional investigation of animal tissue function in normal and pathophysiological conditions.
Unrestricted amount of tissue.
Evaluation of the ability of drugs at modulating different smooth muscle tone can be performed on contractile or relaxant response elicited by pharmacological agents or by electrical field stimulation (EFS).
Evaluation of mRNA by RT-PCR or protein expression, by immunohistochemistry (IHC) or western-blot (WB), in parallel of organ bath studies.
NB: Pelvipham will gladly study the feasibility of performing additional in vitro assays on other species to meet its client’s needs.

ENDPOINTS

Evaluation of the capacity of a drug to inhibit detrusor smooth muscle contractile activity.
Determination of potency (EC50) and efficiency (Emax) of a drug.
Determination of the affinity (pA2) of a drug for a human bladder receptor.

HUMAN TISSUES

Direct investigation of human tissue physiology and pharmacology, thus very relevant in terms of "transposition" to human pathologies.
Evaluation of ex vivo contraction and relaxation processes of various smooth muscles.
Unique opportunity to evaluate the effect of a drug and its mechanism of action on human physiological functions at the preclinical stage of development.
In vitro evaluation of the effect of drugs at modulating smooth muscle tone in normal or pathological conditions.
Determination and comparison of potency (EC50), efficiency (Emax) and tissue selectivity of drugs by performing concentration response curves.
Determination of drug affinity (pA2) for a specific receptor and a specific tissue.

HUMAN TISSUE AVAILABLE

Bladder from control patients (with no known bladder dysfunction according to their medical chart).
Bladder from neurogenic patients
Prostate from control patients
Prostate from patients with benign prostatic hyperplasia
Corpus cavernosum
Seminal vesicles
Other tissues can be obtained upon specific request

SOURCE OF HUMAN TISSUES SAMPLES

Pelvipharm collaborates with public and private hospitals located in Paris and other regions all over France.
The collection and use of tissue is carried out in accordance with the Research Plan, all relevant laws, regulations and codes of practice (including obtaining informed consent of patients in writing and determination of hepatitis and HIV serologies).
Human tissues are transported to Pelvipharm facilities via a specialized carrier ensuring security and adapted condition of storage

HUMAN TISSUE PREPARATION

Sections are excised from the sample of each donor for each experiment.
Tissue strips are mounted isometrically to force transducers in organ bath filled with Krebs-HEPES buffer (37°C; 95% O2 and 5% CO2).
Following amplification, the tension changes are digitalized via a Mac Lab TM/8 using Chart TM 5 software (AD Instruments Ltd, Australia).
Appropriate pharmacological agents and/or electrical field stimulation are then applied on the tissue strips after adequate priming of the tissue.

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Vaginal Atrophy Animal Models
Price on request

OBJECTIVES:

To evaluate vaginal morphological and histological changes in ovariectomized female rat, a relevant preclinical model of menopause
To evaluate the thickness of the vaginal epithelium
To examine the architecture and the cell shape of the vaginal epithelium
Useful for the evaluation of the effect of drugs developed... Show more »

OBJECTIVES:

To evaluate vaginal morphological and histological changes in ovariectomized female rat, a relevant preclinical model of menopause
To evaluate the thickness of the vaginal epithelium
To examine the architecture and the cell shape of the vaginal epithelium
Useful for the evaluation of the effect of drugs developed for menopausal symptoms, including vaginal atrophy, vaginal dryness, lubrication difficulties or dyspareunia.

SUMMARIZED METHODOLOGY:

Adult non-pregnant female Sprague-Dawley rats are ovariectomized bilaterally through a lumbar incision. The ovarian bundles are tied off with 3/0 polyester suture and the ovaries removed. The effectiveness of ovarian removal is checked under binocular microscope. The fascia and the skin are closed using 3/0 polyester suture. Two to four weeks after surgery, the vagina are collected and processed for histological and morphological analysis.

ENDPOINTS:

Vaginal weight
Thickness of the epithelium : surface area and number of epithelial layers
Architecture of the epithelium : shape and morphology of epithelial cells

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Tail Cuff Blood Pressure Monitoring
Price on request

OBJECTIVES

Tail cuff is a common and non-invasive method to measure both systolic blood pressure (SBP) and heart rate (HR) in conscious restrained rats. This method allows repeated SBP measurements to evidence the effect of a chronic drug administration with time and in comparison with vehicle treated animals.

SUMMARIZED... Show more »

OBJECTIVES

Tail cuff is a common and non-invasive method to measure both systolic blood pressure (SBP) and heart rate (HR) in conscious restrained rats. This method allows repeated SBP measurements to evidence the effect of a chronic drug administration with time and in comparison with vehicle treated animals.

SUMMARIZED METHODOLOGY:

Briefly, after training the rat to the tail cuff system, the rat is placed in a restrainer and warmed. A pneumatic pulse sensor is attached to its tail. A cuff is placed around the tail, and slowly inflated above the systolic pressure until it causes pulsations to cease, measured by the piezo-electric pulse sensor. The cuff pressure at which pulsations cease is taken to be the SBP in the tail. HR is determined automatically by counting pulses per unit time.

ENDPOINTS

Systolic blood pressure (SBP)
Heart rate (HR)

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Diuresis Animal Model
Price on request

OBJECTIVES

Urine harvesting using metabolic cages allows the repeated monitoring of diuresis and renal function in rats / mice. Moreover, the urinary excretion rate of a wide variety of substances can be biochemically determined in 24h-urine samples collected on a refrigerated rack (allowing urine collection from +4° down to... Show more »

OBJECTIVES

Urine harvesting using metabolic cages allows the repeated monitoring of diuresis and renal function in rats / mice. Moreover, the urinary excretion rate of a wide variety of substances can be biochemically determined in 24h-urine samples collected on a refrigerated rack (allowing urine collection from +4° down to -15° C) (figure 1), limiting thus the possible degradation of the excreted substance.
SUMMARIZED METHODOLOGY

After a first stay with no urine collection to reduce the stress of the animal on the day of collection, the animals are placed in metabolic cages for a 24 h period to collect urine at a desired temperature from +4° down to -15° C. After recording the total urine volume, 24-h urine samples are centrifuged and supernatant stored for future biochemical determinations.

ENDPOINTS

• 24-h urine volume
• refrigerated urine sample collection

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Micturition Animal Models
Price on request

SUMMARIZED METHODOLOGY FOR SPONTANEOUS MICTURITION EVALUATION:

After a first stay in metabolic cages without data collection to reduce animal stress, the animals are placed in metabolic cages for one or more 24h periods. During each 24h period, the spontaneous micturition is monitored by expelled urine volume weighing by the... Show more »

SUMMARIZED METHODOLOGY FOR SPONTANEOUS MICTURITION EVALUATION:

After a first stay in metabolic cages without data collection to reduce animal stress, the animals are placed in metabolic cages for one or more 24h periods. During each 24h period, the spontaneous micturition is monitored by expelled urine volume weighing by the mean of a weighing device placed underneath, connected to a SartoCollect software (figure 2).

The water/food consumption can also be monitored and 24h-feces or -urine samples can also be collected on ice.
ENDPOINTS:

  • Diuresis
  • Frequency of voiding event per hour
  • Mean volume per voiding event on the overall 24h or per hour
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Cystometry Animal Models
Price on request

OBJECTIVES
* To explore bladder function by measuring intravesical pressure during bladder filling until the point of fullness which elicits a voiding response
* performed in either anaesthetised or conscious animals
* performed in either normal or pathophysiological animal models (OAB, NDO, BPH etc....)

SUMMARIZED... Show more »

OBJECTIVES
* To explore bladder function by measuring intravesical pressure during bladder filling until the point of fullness which elicits a voiding response
* performed in either anaesthetised or conscious animals
* performed in either normal or pathophysiological animal models (OAB, NDO, BPH etc....)

SUMMARIZED METHODOLOGY

A bladder dome catheter is inserted allowing continuous filling of the bladder with saline (50 µl/min for normal rats or 300 µl/min for guinea pigs) and simultaneous measurement of intravesical pressure.

For experiments in conscious animals, the intravesical catheter implantation is performed 48 hours before the cystometry experiment. The free end of the bladder catheter is tunneled subcutaneously, exteriorized at the back of the neck and sutured between the scapulas. Animals are allowed to recover at least for 48 h after surgery until cystometry experiment.

ENDPOINTS

maximal amplitude (MP, mm Hg) and duration of voiding contractions (s)
baseline intravesical pressure (BP, mm Hg)
micturition pressure threshold (PT, mm Hg, intravesical pressure at which voiding is initiated),
intercontraction interval (ICI, s)
voided volume
For chronic pathophysiological models: non-voiding contractions during the bladder filling phase are also analyzed :

amplitude of non-voiding contractions for each micturition cycle(mm Hg),
frequency for each micturition cycle (number of non-voiding contractions per min)

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Animal Behavior Studies
Price on request
  • Unilevel chamber
  • Unilevel pacing chamber
  • Ex copula test
  • Penile reflex
  • Sexual incentive motivation test
  • Bilevel chamber
  • Von Frey behavioural test
  • Unilevel chamber
  • Unilevel pacing chamber
  • Ex copula test
  • Penile reflex
  • Sexual incentive motivation test
  • Bilevel chamber
  • Von Frey behavioural test
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Confocal Microscopy
Price on request

Objectives

Confocal laser scanning microscopy is a technique developed for obtaining high-resolution optical images. This technique is particularly useful to examine double or triple immunofluorescent labelling, developed for example to examine the distribution and localization of different molecules (receptors, peptides,... Show more »

Objectives

Confocal laser scanning microscopy is a technique developed for obtaining high-resolution optical images. This technique is particularly useful to examine double or triple immunofluorescent labelling, developed for example to examine the distribution and localization of different molecules (receptors, peptides, enzymes, etc…) on same tissues.

Summarized methodology

Fluorescent immunocytochemistry using antibodies against molecules of interest is performed on slices of tissue. Then, high-resolution optical images are acquired using confocal laser scanning microscopy which allows the analysis and quantification of the labelling.

Endpoints

  • localisation of molecules of interest
  • number and percentage of double or triple labeled positive cells
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Immunohistochemistry (IHC)
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Objectives

Immunohistochemistry allows evidencing the presence a protein within a tissue section but also its localization within the tissue. This method may help to better characterize the cellular component targeted by an active compound and/or its mechanism of action.

Summarized methodology

Objectives

Immunohistochemistry allows evidencing the presence a protein within a tissue section but also its localization within the tissue. This method may help to better characterize the cellular component targeted by an active compound and/or its mechanism of action.

Summarized methodology

  • Immunohistochemistry can be performed on paraffin-embedded tissue sections or cryosections.
  • Immunostaining can be performed with the use of an enzyme-coupled (HRP or AP) or with a biotinylated secondary antibody when signal amplification (biotin-streptavidin-HRP complex) is needed
  • Immunological reaction is detected using either a colorimetric enzymatic reaction or a fluorescent dye

Endpoints

  • Expression and localization of protein of interest
  • semi-quantitative evaluation of the level of expression of a protein of interest
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Oxidative Stress Assays
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Objectives

Under conditions of oxidative stress, which are associated with aging and a variety of pathological events (including cardiovascular disorders) reactive oxygen species (ROS) production is dramatically increased, resulting in subsequent alteration of membrane lipids, proteins and nucleic acids. In this context, the... Show more »

Objectives

Under conditions of oxidative stress, which are associated with aging and a variety of pathological events (including cardiovascular disorders) reactive oxygen species (ROS) production is dramatically increased, resulting in subsequent alteration of membrane lipids, proteins and nucleic acids. In this context, the balance between superoxide and NO release is of particular interest and could be evaluated using oxidative fluorescence.

The use of fluorescent probes in living tissue constitutes therefore, one of the best way to measure the production of ROS and to localize their formation.

Summarized methodology

Superoxide and NO production is evaluated in freshly harvested unfixed and unfrozen tissue.

Superoxide detection using dihydroethidium (DHE): DHE is a cell-permeable probe which undergoes oxidation in the presence of superoxide leading to the formation of fluorescent ethidium which intercalates within the cell's DNA, staining its nucleus a bright fluorescent red.

NO detection using DAF-FM (4-amino-5-methylamino-2',7'-difluoro fluorescein) diacetate: DAF-FM diacetate is a membrane permeable dye that emits increased fluorescence after reaction with an active intermediate of NO formed during the spontaneous oxidation of NO to NO2- .

Endpoints

  • Baseline superoxide and NO tissue production
  • Stimulated superoxide and NO tissue production using pharmacological agents
  • Identification of the sources of superoxide production by the use of specific inhibitors (i.e. mitochondrial respiratory chain inhibitor rotenone; NADPH oxidase inhibitor diphenylene iodinium (DPI); cyclooxygenase inhibitor indomethacin; or xanthine oxidase inhibitor oxypurinol)
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Histomorphological Analysis
Price on request

Objectives

In addition to organ weighing, the morphological examination of tissue sections gives additional information on the impact of a treatment on the morphology of a tissue.

Summarized methodology

General or specific stains can be applied on various types of tissue and on paraffin sections or cryosections. The... Show more »

Objectives

In addition to organ weighing, the morphological examination of tissue sections gives additional information on the impact of a treatment on the morphology of a tissue.

Summarized methodology

General or specific stains can be applied on various types of tissue and on paraffin sections or cryosections. The stain will be chosen according to the target to be evidenced and the nature of the sample.

Endpoints

  • Qualitative observation of the structure of the tissue.
  • Could be completed by histomorphometric measurements.
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Histomorphometric Analysis
Price on request

Objectives

In addition to organ weighing, and morphological examination, several histomorphometric measurements can be performed to quantitatively evaluate the remodeling of a tissue.

Summarized methodology

Following tissue staining, images are captured using a light microscope (Eclipse E800, Nikon, France) connected... Show more »

Objectives

In addition to organ weighing, and morphological examination, several histomorphometric measurements can be performed to quantitatively evaluate the remodeling of a tissue.

Summarized methodology

Following tissue staining, images are captured using a light microscope (Eclipse E800, Nikon, France) connected to a video camera (Digital camera DXM1200, Nikon, France), and histomorphometric measurements are performed by computerized image analysis system (NIS-elements software®).

Endpoints

Different parameters can be evaluated according to the type of tissue to be examined

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Western Blot
Price on request

Objectives

Western blot allows the determination of the expression of a protein in its native or active form in tissues and helps to better understand the mechanism of action of an active compound.

Summarized methodology

Several steps are to be taken into account in order to optimize the results to be obtained:

-... Show more »

Objectives

Western blot allows the determination of the expression of a protein in its native or active form in tissues and helps to better understand the mechanism of action of an active compound.

Summarized methodology

Several steps are to be taken into account in order to optimize the results to be obtained:

  • Tissue harvesting and homogeneization (proteases and phosphatases inhibitors when necessary).
  • Protein separation on denaturing SDS-polyacrylamide gel
  • Primary and secondary antibodies (HRP-linked or biotinylated followed by the addition of streptavidin-HRP) concentrations and conditions of incubation

Endpoints

The proteins of interest are detected by enhanced chemiluminescence and visualized by immediate exposure to autoradiographic film (figure 1). Each sample is assayed in duplicate or triplicate and densitometric results are averaged per animal, and the results are normalized to the relative density of the internal control.

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Spectrophotometry
Price on request

Objectives

Spectrophotometric assays allows the determination of the concentration of a substance of interest in various biological samples (urine, blood, plasma, tissue) and helps to better understand the mechanism of action of an active compound.

Summarized methodology

A wide range of assays have been validated... Show more »

Objectives

Spectrophotometric assays allows the determination of the concentration of a substance of interest in various biological samples (urine, blood, plasma, tissue) and helps to better understand the mechanism of action of an active compound.

Summarized methodology

A wide range of assays have been validated based on direct colorimetric reactions or immuno-enzymatic detection (EIA / ELISA).

Pelvipharm is used to collect various sample types (urine, blood, plasma, tissue), while taking special care to adopt an appropriate storage of samples and to perform pre-analytical steps (extraction when needed) before any biochemical spectrophotometric assay.

Absorbance reading is performed using a Molecular Devices microplate reader (Spectramax 190) associated to SoftMax® Pro microplate analysis software.

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Animal Urine Collection
Price on request

Objectives

The collection of blood / urine / tissue following compound administration can allow the identification of a mechanism of action performing biochemical and/or histological analysis. Collected samples can also be sent to Pelvipharm’s client if needed.

Summarized methodology

Objectives

The collection of blood / urine / tissue following compound administration can allow the identification of a mechanism of action performing biochemical and/or histological analysis. Collected samples can also be sent to Pelvipharm’s client if needed.

Summarized methodology

  • Plasma / blood collection: blood samples (venous / arterial) are collected either in conscious or anesthetized animals, immediately placed at 4°C and centrifuged to collect plasma samples.
  • Urine collection: Urine harvesting can be performed using metabolic cages and refrigerated collection rack, or by direct vesical collection.
    • Care is particularly taken to appropriately store the blood/plasma and urine samples (liquid nitrogen, -80°C, -20°C, 4°C) and to add appropriate preservatives according the future use of these samples.
  • Tissue collections:
    • Various tissues can be harvested in freshly euthanized animals :
    • central nervous system: brain, spinal cord
    • cardiovascular / pulmonary system: heart, vessels, lungs
    • urinary system: kidney, bladder, urethra, prostate
    • sexual system: corpora cavernosa, seminal vesicle, vagina, clitoris, ...
    • other systems: striated muscle, digestive tract, liver, …
    • Tissues can be prepared for in vitro, biochemical or histological purposes as follows :
    • dissected at 4°C and mounted in organ bath chambers for reactivity studies.
    • snap frozen in liquid nitrogen for future homogeneization and biochemical determinations.
    • embedded in paraffin or cryopreserving milieu (with or without preliminary fixation) for future histological studies.

Endpoints

  • Organ weighing and volume evaluation
  • Sample collection
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Animal Blood Collection
Price on request

Objectives

The collection of blood / urine / tissue following compound administration can allow the identification of a mechanism of action performing biochemical and/or histological analysis. Collected samples can also be sent to Pelvipharm’s client if needed.

Summarized methodology

Objectives

The collection of blood / urine / tissue following compound administration can allow the identification of a mechanism of action performing biochemical and/or histological analysis. Collected samples can also be sent to Pelvipharm’s client if needed.

Summarized methodology

  • Plasma / blood collection: blood samples (venous / arterial) are collected either in conscious or anesthetized animals, immediately placed at 4°C and centrifuged to collect plasma samples.
  • Urine collection: Urine harvesting can be performed using metabolic cages and refrigerated collection rack, or by direct vesical collection.
    • Care is particularly taken to appropriately store the blood/plasma and urine samples (liquid nitrogen, -80°C, -20°C, 4°C) and to add appropriate preservatives according the future use of these samples.
  • Tissue collections:
    • Various tissues can be harvested in freshly euthanized animals :
    • central nervous system: brain, spinal cord
    • cardiovascular / pulmonary system: heart, vessels, lungs
    • urinary system: kidney, bladder, urethra, prostate
    • sexual system: corpora cavernosa, seminal vesicle, vagina, clitoris, ...
    • other systems: striated muscle, digestive tract, liver, …
    • Tissues can be prepared for in vitro, biochemical or histological purposes as follows :
    • dissected at 4°C and mounted in organ bath chambers for reactivity studies.
    • snap frozen in liquid nitrogen for future homogeneization and biochemical determinations.
    • embedded in paraffin or cryopreserving milieu (with or without preliminary fixation) for future histological studies.

Endpoints

  • Organ weighing and volume evaluation
  • Sample collection
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Animal Tissue Procurement
Price on request

Objectives

The collection of blood / urine / tissue following compound administration can allow the identification of a mechanism of action performing biochemical and/or histological analysis. Collected samples can also be sent to Pelvipharm’s client if needed.

Summarized methodology

Objectives

The collection of blood / urine / tissue following compound administration can allow the identification of a mechanism of action performing biochemical and/or histological analysis. Collected samples can also be sent to Pelvipharm’s client if needed.

Summarized methodology

  • Plasma / blood collection: blood samples (venous / arterial) are collected either in conscious or anesthetized animals, immediately placed at 4°C and centrifuged to collect plasma samples.
  • Urine collection: Urine harvesting can be performed using metabolic cages and refrigerated collection rack, or by direct vesical collection.
    • Care is particularly taken to appropriately store the blood/plasma and urine samples (liquid nitrogen, -80°C, -20°C, 4°C) and to add appropriate preservatives according the future use of these samples.
  • Tissue collections:
    • Various tissues can be harvested in freshly euthanized animals :
    • central nervous system: brain, spinal cord
    • cardiovascular / pulmonary system: heart, vessels, lungs
    • urinary system: kidney, bladder, urethra, prostate
    • sexual system: corpora cavernosa, seminal vesicle, vagina, clitoris, ...
    • other systems: striated muscle, digestive tract, liver, …
    • Tissues can be prepared for in vitro, biochemical or histological purposes as follows :
    • dissected at 4°C and mounted in organ bath chambers for reactivity studies.
    • snap frozen in liquid nitrogen for future homogeneization and biochemical determinations.
    • embedded in paraffin or cryopreserving milieu (with or without preliminary fixation) for future histological studies.

Endpoints

  • Organ weighing and volume evaluation
  • Sample collection
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Biospecimens
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Spectroscopy
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Animal Learning, Memory, and Behavior Tests
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Clinical Research
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Imaging & Spectroscopy
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Optical Microscopy
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Veterinary Research & Diagnostics
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Animal Model in vivo Analyses
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Cellular Health & Metabolism Assays
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Reproductive System Animal Models
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Pharmacology & Toxicology
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Biochemistry & Molecular Biology
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Human Biospecimen Processing
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CNS/Neurology Animal Models
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Cells and Tissues
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Animal Cognition & Behavior Tests
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Animal Biospecimen Collection
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Animal Models and Studies
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Biology
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Animal Biospecimens
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Animal Physiology Analyses
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Clinical and Anatomic Pathology
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Urogenital Animal Models
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Fluorescence-Based Microscopy
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Spinal Cord Injury
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Spinal Cord Injury Models
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Contract Research
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Cell-Based Assays
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Clinical Laboratory Services
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Microscopy
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Animal Models of Disease
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Protein Services
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Immunostaining
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Protein Expression Visualization
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Veterinary Laboratory Services
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