Olympic Protein Technologies is a contract research organization focused on providing high quality protein science services for clients and partners. The quality of proteins are vital to the success of experiments that form the foundation of the discovery and development of therapeutics. Our staff has a wealth of collective experience in top-tier biotechnology companies successfully designing, producing and characterizing protein therapeutic candidates as well as critically important antigens and reagents. This experience includes solving problems associated with the expression/purification of challenging proteins. The aim of our team is to leverage this knowledge to provide the best experience and outcomes for our partners and clients by providing quality science delivered with full engagement and the highest standards, integrity, and data security.
We currently offer construct design, expression, protein... Show more »
Olympic Protein Technologies has not listed any services.
Consultation, quantitative binding and kinetic constant determinations, high throughput screens, epitope binning, and assays for discovery work and regulatory submissions for a range of molecular types (small molecules and large molecules including ligands, receptors, mAbs, ADCs, bispecifics, and peptides)
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DNA Construction and Cloning
Encoding Native or Engineered Proteins and Antibodies
New Design
3 weeks
Design and synthesis of new DNA encoding protein of interest in an expression vector
Construction of Expression Vector
2 weeks
Subcloning of existing protein-encoding DNA into an expression vector
Hybridoma Antibody Variable Domain Sequencing
2-3 weeks
Determine nucleotide sequences of variable domains from antibody heavy and light chains derived from hybridomas
Test for Transient Expression in Mammalian Cells
2 weeks
Small scale test for expression of new construct in mammalian cell system
Scale Up Transient Expression in Mammalian Cells
2 weeks
Constructs that pass the expression test are scaled up in ranges spanning culture volumes of 100 ml to 8 liters.
Test for Expression in Bacterial Cells
1 week
Small scale test for expression of new construct in bacterial cell system
Scale Up Expression in Bacterial Cells
1 week
Constructs that pass the expression test are scaled up in ranges spanning culture volumes of 100 ml to several liters
Purification from Mammalian Cells
2 Weeks
Purification and characterization of protein expressed from mammalian cells
Purification from Bacterial Cells
2 weeks
Purification and characterization of protein expressed from bacterial cells
Antibody Panels
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Expression and purification of panels of antibodies -... Show more »
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Modifications include biotinylations and the conjugation of fluorescent dyes and other tags
Structure-Based Analysis
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Analysis incorporating structural and bioinformatic aspects for translating design goal elements to a list of testable protein variants
Single or Multiple Point Mutants and Screening
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Focused exploration of protein variants
Library Design and Screening
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Broad exploration of protein variants
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