Olympic Protein Technologies is a contract research organization focused on providing high quality protein science services for clients and partners. The quality of proteins are vital to the success of experiments that form the foundation of the discovery and development of therapeutics. Our staff has a wealth of collective experience in top-tier biotechnology companies successfully designing, producing and characterizing protein therapeutic candidates as well as critically important antigens and reagents. This experience includes solving problems associated with the expression/purification of challenging proteins. The aim of our team is to leverage this knowledge to provide the best experience and outcomes for our partners and clients by providing quality science delivered with full engagement and the highest standards, integrity, and data security. We currently offer construct design, expression, protein production & characterization, biomolecular interaction analysis, and protein engineering services. We also leverage our network of protein science contacts to provide access to other types of analyses, such as advanced biophysical technologies, for your project. As we grow we will build on our existing capabilities while broadening our offerings to include other protein science services. We are located in Seattle Washington USA.
Transient and Stable (pool) recombinant protein production.
Scale from 30 mls to 30 liters.
Deliverable: purified and characterized protein, sterile filtered supernatant, or cell pellets.
Consultation, quantitative binding and kinetic constant determinations, high throughput screens, epitope binning, and assays for discovery work and regulatory submissions for a range of molecular types (small molecules and large molecules including ligands, receptors, mAbs, ADCs, bispecifics, and peptides)
Affinity Determination by Biacore SPR
Determination of affinity and kinetic parameters for protein-protein and protein-ligand interactions
Custom Biacore Reagent Generation
Custom reagents for SPR and assay studies
Hybridoma Antibody Variable Domain Sequencing
Determine nucleotide sequences of variable domains from antibody heavy and light chains derived from hybridomas
Test for Expression in Bacterial Cells
Small scale test for expression of new construct in bacterial cell system
Scale Up Expression in Bacterial Cells
Constructs that pass the expression test are scaled up in ranges spanning culture volumes of 100 ml to several liters
Modifications include biotinylations and the conjugation of fluorescent dyes and other tags
Test for Transient Expression in Mammalian Cells
Small scale test for expression of new construct in mammalian cell system
Scale Up Transient Expression in Mammalian Cells
Constructs that pass the expression test are scaled up in ranges spanning culture volumes of 100 ml to 8 liters.
Purification from Mammalian Cells
Purification and characterization of protein expressed from mammalian cells
Purification from Bacterial Cells
Purification and characterization of protein expressed from bacterial cells
Expression and purification of panels of antibodies - various scales available
Refolding of proteins from E coli inclusion bodies
DNA Construction and Cloning
Encoding Native or Engineered Proteins and Antibodies
Design and synthesis of new DNA encoding protein of interest in an expression vector
Construction of Expression Vector
Subcloning of existing protein-encoding DNA into an expression vector
Analysis incorporating structural and bioinformatic aspects for translating design goal elements to a list of testable protein variants
Single or Multiple Point Mutants and Screening
Focused exploration of protein variants
Library Design and Screening
Broad exploration of protein variants
Recombinant Protein Expression Services
"My experience with Olympic Protein Technologies was very positive. From the initial interaction, I felt confident in the abilities of this company to effectively research, plan and execute this project which involved cloning, expression and purification of a protein using E. coli for recombinant protein expression. The research plan that was proposed was quite similar to how I would have carried out the work in house, and was based on available references and years of experience. The final results of the project were quite satisfactory, and even with some challenges during the execution phase of the project, OPT kept me informed and updated on progress. I will definitely work with this company again."
Olympic Protein Technologies has not received any endorsements.