Olympic Protein Technologies

DNA Construction and Cloning
Encoding Native or Engineered Proteins and Antibodies

New Design
3 weeks
Design and synthesis of new DNA encoding protein of interest in an expression vector

Construction of Expression Vector
2 weeks
Subcloning of existing protein-encoding DNA into an expression vector

Hybridoma Antibody Variable Domain Sequencing
2-3 weeks
Determine nucleotide sequences of variable domains from antibody heavy and light chains derived from hybridomas

Test for Transient Expression in Mammalian Cells
2 weeks
Small scale test for expression of new construct in mammalian cell system

Scale Up Transient Expression in Mammalian Cells
2 weeks
Constructs that pass the expression test are scaled up in ranges spanning culture volumes of 100 ml to 8 liters.

Test for Expression in Bacterial Cells
1 week
Small scale test for expression of new construct in bacterial cell system

Scale Up Expression in Bacterial Cells
1 week
Constructs that pass the expression test are scaled up in ranges spanning culture volumes of 100 ml to several liters

Purification from Mammalian Cells
2 Weeks
Purification and characterization of protein expressed from mammalian cells

Purification from Bacterial Cells
2 weeks
Purification and characterization of protein expressed from bacterial cells

Antibody Panels
Inquire
Expression and purification of panels of antibodies - various scales available

Protein Refolding
3 weeks
Refolding of proteins from E coli inclusion bodies

Inquire
Modifications include biotinylations and the conjugation of fluorescent dyes and other tags

Various formats available

Structure-Based Analysis
Inquire
Analysis incorporating structural and bioinformatic aspects for translating design goal elements to a list of testable protein variants

Single or Multiple Point Mutants and Screening
Inquire
Focused exploration of protein variants

Library Design and Screening
Inquire
Broad exploration of protein variants