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OcellO

Leiden, NL

OcellO B.V. is a privately owned contract research organisation, with approx. 20 employees, founded in 2011, offering compound testing services using a proprietary high throughput and high content 3D screening platform.

OcellO is an innovative biotech company offering CRO services for the drug discovery industry that can significantly improve the efficiency of lead development. OcellO excels in the development of clinically relevant in vitro human micro-tissue models that can be used for the screening and profiling of small molecules and biologics. The analysis of these models, many of which use patient-derived material, is enabled by OcellO’s unique high throughput 3D imaging and phenotypic profiling technology. As well as providing measurements of cell growth and viability, phenotypic profiling allows the measurement of more complex biology that better correlates with clinical endpoints. Furthermore, compounds... Show more »

OcellO B.V. is a privately owned contract research organisation, with approx. 20 employees, founded in 2011, offering compound testing services using a proprietary high throughput and high content 3D screening platform.

OcellO is an innovative biotech company offering CRO services for the drug discovery industry that can significantly improve the efficiency of lead development. OcellO excels in the development of clinically relevant in vitro human micro-tissue models that can be used for the screening and profiling of small molecules and biologics. The analysis of these models, many of which use patient-derived material, is enabled by OcellO’s unique high throughput 3D imaging and phenotypic profiling technology. As well as providing measurements of cell growth and viability, phenotypic profiling allows the measurement of more complex biology that better correlates with clinical endpoints. Furthermore, compounds with the optimum therapeutic profile or optimum target specific profile can be selected to enable a better ranking of compounds at early stage in the drug development process.

At OcellO we believe that sound collaboration and cutting edge science can speed up selection and development of clients lead compounds. We also welcome opportunities for partnerships, for example in Horizon 2020 projects. We are involved in several grant projects and are happy to work together!

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OcellO has not listed any services.

3D Cell Culture Models
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3D Cell-Based Assays
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OcellO specialises in generating models that closely recapitulate both healthy and disease phenotypes. Healthy tissues show normal functions, such as salt and water transport in kidney epithelial tubules or production of milk proteins by breast epithelial cells – functions that are lost in 2D cultures. In our disease models,... Show more »

OcellO specialises in generating models that closely recapitulate both healthy and disease phenotypes. Healthy tissues show normal functions, such as salt and water transport in kidney epithelial tubules or production of milk proteins by breast epithelial cells – functions that are lost in 2D cultures. In our disease models, tissues also show the expected disruption of phenotype and function that is associated with patient disease; cancer cells demonstrate dysplasia and become invasive, whereas epithelial cells from patients with cystic diseases show abnormal cyst formation. A more accurate simulation of the in vivo disease phenotype allows you to select more better quality molecules to progress through your discovery pipeline.

Using our disease models we can obtain various functional and clinical relevant readouts:

  • Proliferation, Migration and invasion
  • apico-basal polarity, dysplasia, lumen formation
  • Cyst swelling and transporter function

Over 300 models are available for screening of your compounds:

  • It is also possible to screen your compounds in panels of assays:
  • Panel of 30 patient-derived colorectal cancer and wt-organoids
  • Panel of 50 breast cancer cell lines
  • Lung, prostate and kidney carcinomas, osteosarcoma
  • Polycystic Kidney Disease

In these models we can show whether the candidate compounds:

  • Restore the disease phenotype to a healthy state
  • Reduce the malignancy of cancer cells (inhibit invasion, inhibt growth or kill cells)
  • Increase sensitivity to existing anti-cancer therapies (drug combinations)

Advantage of testing compounds in a disease model:

  • Unbiased testing
  • Possibilities to identify new classes of compounds
  • Possibilities to identify new targets and pathways
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Organoids
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OcellO is the only CRO with a license (from Foundation Hubrecht Organoid Technology, founded by Hans Clevers) to offer screening in human gastrointestinal organoids. OcellO has over 100 well characterised organoids, including approximately 10 pairs of normal and colorectal cancer organoids derived from the same patient. Compounds... Show more »

OcellO is the only CRO with a license (from Foundation Hubrecht Organoid Technology, founded by Hans Clevers) to offer screening in human gastrointestinal organoids. OcellO has over 100 well characterised organoids, including approximately 10 pairs of normal and colorectal cancer organoids derived from the same patient. Compounds are evaluated for effects on organoid growth, killing differentiatrion, apoptosos and other phenotypic end-points using OcellO's proprietary high content 3D screening platform.

Unique advantages:
Consistency of cell lines, but retaining characteristics of patient tissues
Compare compound effects on normal and tumor samples to determine therapeutic window/flag toxicity issues
Screen in panels of genetically well characterised patient lines for patient stratification/in vitro pre clinical 'trials'

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Immuno-oncology Assays
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When testing immunotherapies, it is often critical to assess the functional cytotoxicity and infiltration capacity. In addition, immunomodulatory effects of new drugs in combination with already approved ones needs to be carefully assessed before these treatment modalities reach patients. However there are unlimited possibilities... Show more »

When testing immunotherapies, it is often critical to assess the functional cytotoxicity and infiltration capacity. In addition, immunomodulatory effects of new drugs in combination with already approved ones needs to be carefully assessed before these treatment modalities reach patients. However there are unlimited possibilities of new combinations, especially in the view of synergy effect observed with double checkpoint inhibition blockade. OcellO has developed a unique screening platform for immuno-oncology. This allows for efficient and cost effective testing of these combinations in assays that yield functional data on the tumour – immune system interplay.

OcellO’s screening platform is based on 3D co-cultures of cancer and immune cells. This 3D environment allows the different cell types to engage in a more realistic setting than when growing the cells in monolayer or mixing them in a suspension culture. Our proprietary OMiner® software platform enables discrimination of i) immune-tumour cell interactions and ii) drug-effects on the tumour and immune cells. This information, which cannot be obtained using conventional biochemical measurements, enables acquiring and automatic quantification of spatially resolved information that is of a critical importance for immuno-oncology studies.

Key advantages:

  • Automated analysis and robust quantification of T cell activation and invasion
  • Functional read-outs: active migration, association with tumoroids, cancer killing
  • Physiologically relevant micro-environment
  • Visualization of T cell interaction with the cancer
  • HLA-matched cell types
  • Enabling immuno-modulator combinations and synergy studies

OcellO offers cancer immunotherapy developers a new, highly powerful tool to select the most promising drugs at a very early stage in drug development. The availability of this new tool will empower the immunotherapy field, allowing the speedy progression of novel drugs towards clinical development – ultimately benefitting cancer patients.

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Compound Profiling
Price on request

The strength of OcellO’s 3D screening platform lies within the multi-dimensional, multi-parametric profiling of the microtissues by using our proprietary software toolset. By combining up to 900 different feature measurements, we can distinguish within one single assay between multiple different compound targets and address their... Show more »

The strength of OcellO’s 3D screening platform lies within the multi-dimensional, multi-parametric profiling of the microtissues by using our proprietary software toolset. By combining up to 900 different feature measurements, we can distinguish within one single assay between multiple different compound targets and address their specificity, mode of action and off-target effects.

Output

  • Dose-response curves
  • Compound classification
  • Insight into target specificity & MOA
  • Polypharmacology

Example: different compounds project differently in phenotypic space

These anti-cancer compounds are all ‘hits’ in viability assays but actually induce very different phenotypes. Compounds inducing specific phenotypes, such as reduced invasion or growth, can be identified and selected. Compounds that target the same pathway induce the same phenotype.

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Cell-Based Compound Screening
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Biology
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Lead Identification and Validation
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Hit Identification
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Drug Discovery & Development
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Cell and Tissue Culture
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Cell and Tissue Culture Services

Cell and Tissue Culture Services

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Cells and Tissues
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Specialized Cell-Based Assays
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Pharmacology & Toxicology
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In vitro Disease Models
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Immunoassays
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Bioanalysis
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Cell-Based Assays
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Drug Discovery
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Mammalian Cell Culture
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High Content Screening
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High content analysis of 3D tissue culture models

OcellO specialises in generating 3D tissue culture models that closely recapitulate both healthy and disease phenotypes. Healthy tissues show normal functions, such as salt and water transport in kidney epithelial tubules or production of milk proteins by breast epithelial... Show more »

High content analysis of 3D tissue culture models

OcellO specialises in generating 3D tissue culture models that closely recapitulate both healthy and disease phenotypes. Healthy tissues show normal functions, such as salt and water transport in kidney epithelial tubules or production of milk proteins by breast epithelial cells – functions that are lost in 2D cultures. In our disease models, tissues also show the expected disruption of phenotype and function that is associated with patient disease; cancer cells demonstrate dysplasia and become invasive, whereas epithelial cells from patients with cystic diseases show abnormal cyst formation. A more accurate simulation of the in vivo disease phenotype allows you to select more better quality molecules to progress through your discovery pipeline.

Using our disease models we can obtain various functional and clinical relevant readouts:

Proliferation, Migration and invasion
apico-basal polarity, dysplasia, lumen formation
Cyst swelling in a model of Polycystic Kidney Disease
Over 300 models are available for screening of your compounds:

It is also possible to screen your compounds in panels of assays:
ex vivo PDX 3D cultures
Panel of 100 patient-derived colorectal cancer and matching wt-organoids
Panel of 50 breast cancer cell lines
Lung, prostate and kidney carcinomas, osteosarcoma
polycystic kidney disease (PKD) assay using kidney collecting duct cell 3D cyst culture

In these models we can show whether the candidate compounds:
Restore the disease phenotype to a healthy state
Reduce the malignancy of cancer cells (inhibit invasion, inhibt growth or kill cells)
Increase sensitivity to existing anti-cancer therapies (drug combinations)
Advantage of testing compounds in a disease model:

Unbiased testing
Possibilities to identify new classes of compounds
Possibilities to identify new targets and pathways

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3D PDX Models
Price on request

ex vivo PDX

Ex vivo/in vitro patient derived xenograft 3D culture screening. High throughput and high content assays using the Charles River/Oncotest PDX collection. Primary PDX tumour is dissociated and allowed to reform as tumoroids in 3D in natural ECM rich hydrogels. Effects of compounds on tumour growth, killing,... Show more »

ex vivo PDX

Ex vivo/in vitro patient derived xenograft 3D culture screening. High throughput and high content assays using the Charles River/Oncotest PDX collection. Primary PDX tumour is dissociated and allowed to reform as tumoroids in 3D in natural ECM rich hydrogels. Effects of compounds on tumour growth, killing, apoptosis, invasion and other phenotypic endpoints are evaluated using OcellO's high content platform.

Key advantages:
- testing in primary tumour
- direct translation to in vivo PDX model (and follow up in the same PDX model at partner CRO)

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In vitro Polycystic Kidney Disease Models
Price on request

Mouse collecting duct cells are cultured in protein hydrogel in 3D to form cystic structures. The effects of compounds on cyst growth and swelling are evaluated using OcellO's high throughput screening platform. Readouts include average cyst size, cyst wall thickness, cytotoxicity.

Unique advantages:
Evaluates compound... Show more »

Mouse collecting duct cells are cultured in protein hydrogel in 3D to form cystic structures. The effects of compounds on cyst growth and swelling are evaluated using OcellO's high throughput screening platform. Readouts include average cyst size, cyst wall thickness, cytotoxicity.

Unique advantages:
Evaluates compound effects on numerous cysts charactaristics in 3D
Discriminates selective inhibitors of cysts swelling from cytotoxic compounds

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Cell-Based Screening Methods
Price on request

Cell-Based Screening Methods Services

Cell-Based Screening Methods Services

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