OakLabs Bio Inc.

• Min. samples: 8
• Turnaround time: 2 weeks
• Species: Arabidopsis, C. elegans, Drosophila, Human, Human FFPE, Mouse, Rat, Rice, S. scrofa, Yeast, Zebrafish

With our ArrayXS product line, we provide experimentally validated Agilent microarrays for genome-wide gene expression analysis. Customers worldwide appreciate this comprehensive service and have used the data to achieve top ranking publications.

Service package includes: 1. Gene expression analysis - all-in service: processing and hybridization of RNA samples on ArrayXS 8x60K Agilent microarrays, 2. Data analysis including statistics, outlier analysis and visualization of differentially expressed genes. A pathway analysis is available on request.

RNA sample requirements: at least 500ng total RNA per sample is required for RNA quality control and microarray analysis; ideally, total RNA samples have a concentration of 20 – 200 ng/ul; if required, smaller amounts of starting material can also be processed for microarray analysis.

We are looking forward to discussing your gene expression analysis project with you!

• Min. samples: 8
• Turnaround time: from 2 weeks (preconfigured) to 8 weeks (custom)
• No. of genes: from >500 (S package) to 1.500+ (XL package)
• Species: preconfigured sets for human, mouse, rat, all others as custom

Our Array-to-Go service provides you with a comprehensive analysis of relevant genes of a biological process or a disease. It was developed towards highest sensitivity to compete with qPCR.

Are you wondering what’s the optimal mean to access just a few pathways or to study the activity of genes relevant in specific diseases or biological processes? With Array-to-Go we have the perfect solution for you. You can choose from 40 preconfigured Array-to-Go compilations for human, mouse and rat or simply have your Array-to-Go custom-tailored for any species with up to 2,000 of your genes of interest. Send us your samples and receive statistically analysed data two weeks later.

focus on certain pathways or genes relevant in specific diseases
benefit from lower costs and higher information value compared to qPCR-based gene set panels
choose from a broad range of pre-configured Array-to-Go compilations for human, mouse and rat
get most out of your budget with different Array-to-Go formats representing various numbers of genes
have an Array-to-Go custom designed for any genes in any species at no charge
get a comprehensive data analysis for free
measure transcripts as sensitively as with qPCR with just 500ng total RNA

Service package includes: 1. Gene expression analysis - all-in service for up to 2,000 genes of interest 2. Data analysis including statistics, outlier analysis and visualization of differentially expressed genes.

We are looking forward to discussing your gene expression analysis project with you!

• Min. samples: 8
• Turnaround time: from 2 weeks
• Species: Arabidopsis, C. elegans, Drosophila, Human, Mouse, Rat, Rice, S. scrofa, Zebrafish

OakLabs single cell gene expression analysis services starts from 5-10 cells in stabilization buffer and includes a pre-amplification, labeling, a proven Agilent microarray from OakLabs' ArrayXS line, hybridization, scanning, and data extraction. Raw data are provided in text format.

Data analysis is available and includes statistics, outlier analysis and visualization of differentially expressed genes. A pathway analysis is available on request.

We are looking forward to discussing your gene expression analysis project with you!

• Turnaround time: 2 weeks
• cDNA synthesis: 7-12 USD
• qPCR service at technical triplicate: 7-12 USD
• TaqMan Probes: 180 USD (per gene; sufficient for 40 samples)

Our MIQE compliant RT-qPCR workflow is performed using state-of-the-art technologies. For each project, we carefully evaluate the optimal qPCR workflow starting from experimental design and assay design, over validation of assays and reference genes to reaction setup and data analysis.

OakLabs supports your qPCR projects with an all-round care free package:

• advice on experimental design
• RNA isolation
• quality control your total RNA (Nanodrop, Bioanalyzer)
• primer and probe design
• assay validation and optimization
• reverse transcription
• controls (NTC,-RT)
• run your samples in triplicates
• data report
• statistical analysis

We provide miRNA Services using the proven miRNA microarrays from Agilent for human, mouse or rat.

The service starts from 300ng total RNA (enrichment of small RNA is not required) and includes RNA QC with the Agilent Bioanalyzer, the miRNA microarray, RNA labeling, hybridization, scanning, and data extraction. Raw data are provided in Excel format.

Data analysis is available and includes statistics, outlier analysis and visualization of differentially expressed genes.

RNA isolation, RNA extraction and RNA purification using appropriate kits from Qiagen kits including DNase digestion and QC with the Agilent Bioanalyzer and Nanodrop photometer.

RNA Expression Profiling in FFPE and LCM samples using ArrayXS Human FFPE

• available with our gene expression analysis service on ArrayXS Human FFPE
• further details available on our website

With PCRdrive you can access a free online platform which includes all the steps of a PCR:

• primer design
• ordering of reagents
• documentation
• sharing with others

Try out our primer design feature at http://www.pcrdrive.com

OakLabs is a leading provider of gene expression analysis services. With ArrayXS, we offer you a perfect tool for genome-wide gene expression analysis.

maximise the information value by detecting mRNA and ncRNA genome-wide
receive high quality data with an exceptional dynamic range of >5 logs
interprete results easily with visualised differential expression of relevant pathways in maps
available for the major model organisms and even more upon request
benefit from highest reliability, reproducability and sensitivity of our experimentally validated microarrays

The ArrayXS service package includes:

1. Gene expression analysis - all-in service: processing and hybridization of RNA samples on ArrayXS 8x60K Agilent microarray
2. Data analysis: statistical analysis of two groups of 4 replicate samples, pathway realization.

Default turnaround time: 2 weeks

Additional Information:
RNA sample requirements: RNA sample shipping within the next six months after order placement; at least 500ng total RNA per sample is required for RNA quality control and microarray analysis; ideally, total RNA samples have a concentration of 20 – 200 ng/ul; if required, smaller amounts of starting material can also be processed for microarray analysis.

ArrayXS Microarrays are 8x60K Agilent microarrays and offer you the following benefits:

• maximise the information value by detecting mRNA and ncRNA genome-wide
• receive high quality data with an exceptional dynamic range of >5 logs
• interprete results easily with visualised differential expression of relevant pathways in maps
• available for the major model organisms and even more upon request
• benefit from highest reliability, reproducibility and sensitivity of our experimentally validated microarrays

ArrayXS Microarrays are exclusively available at OakLabs for the following species:

• A. thaliana
• C. elegans
• D. melanogaster
• Human
• Mouse
• Rat
• Zebrafish

With OakLabs' gene expression analysis services, you will get all-round carefree packages from tissue samples to visualised data guarantee a straightforward interpretation of the results.
With our fast turn-around times of just two weeks you will get the basis to publish your research sooner.
Our expert team tailors solutions to adapt our service to your specific needs. Regardless of whether you need a custom microarray design for your study species, focus on your few thousand genes of interest in any species, or need an exceptional data analysis or visualisation, we will make it possible.

For every gene expression service, we offer you a number of comprehensive data analyses tailored to your project: from heat maps over pathway analyses to scatter plots. Thus, you can interpret your data quickly and use them in your publication.

• development of an experimentally validated custom microarray (ArrayXS) for any species with available transcript sequences
• from 3,000 USD (ArrayXS for most eukaryotes) - 12,000 USD (ArrayXS for prokaryotes)