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NUSeq Core Facility

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Chicago, Illinois, US

About NUSeq Core Facility

The NUSeq Core Facility at Northwestern University is a shared resource facility that serves investigators at Northwestern University, affiliated institutions, and external academic and commercial organizations.

The facility provides a wide range of genomic/genetic technologies, including next-generation... Show more »

The NUSeq Core Facility at Northwestern University is a shared resource facility that serves investigators at Northwestern University, affiliated institutions, and external academic and commercial organizations.

The facility provides a wide range of genomic/genetic technologies, including next-generation sequencing, microarray, PCR, gene expression profiling, genotyping, methylomics, epigenomics, DNA-protein interaction, 3D genome and DNA accessibility, and metagenomics. Other genomic services include automated DNA extraction, DNA/RNA concentration measurement and quality control.

On the bioinformatics front, the Core provides in-house data analysis and data mining services for genomics data generated in the core or externally.

Our trained staff can assist you with every step of your project, from design to analysis.

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Our Services (33)


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Illumina Sequencing

Starting at $375.00 per library

Sequencing of in-house or externally prepared libraries. For externally prepared libraries, library QC and quantification is required and additional. Turnaround time is two weeks. For users not affiliated with Northwestern/UChicago/UIC, there are two tiers of pricing available, i.e., external academic and industrial. Pricing... Show more »

Sequencing of in-house or externally prepared libraries. For externally prepared libraries, library QC and quantification is required and additional. Turnaround time is two weeks. For users not affiliated with Northwestern/UChicago/UIC, there are two tiers of pricing available, i.e., external academic and industrial. Pricing varies based on run mode and amount of data requested (the starting price is for the lowest amount of sequencing that can be requested). Deliverables are FASTQ files containing Illumina reads passing filter. Bioinformatics data analysis is available upon request.

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Chromatin Immunoprecipitation (ChIP) Assays

Price on request

ChIP-seq is a genomics technology developed to map binding sites of a DNA-interacting protein across the genome. Examples of DNA-interacting proteins include transcription factors, histones, and enzymes for DNA repair and modification. A common application of ChIP-seq is to locate transcription factor binding patterns under... Show more »

ChIP-seq is a genomics technology developed to map binding sites of a DNA-interacting protein across the genome. Examples of DNA-interacting proteins include transcription factors, histones, and enzymes for DNA repair and modification. A common application of ChIP-seq is to locate transcription factor binding patterns under different conditions, such as development stages or pathological conditions.

ChIP-seq starts with covalent cross-linking of DNA with interacting proteins, then shearing of chromatin into fragments, followed by enrichment of the protein of interest with its bound DNA by immunoprecipitation using an antibody specific for the protein. Subsequently, after dissociating the enriched protein-DNA complex, the released DNA fragments are subjected to sequencing. One key experimental factor in the ChIP-Seq process is the quality of the antibody used in the enrichment step, as the use of a poor-quality antibody can lead to high experimental noise due to non-specific precipitation of DNA fragments.

NUSeq accepts ChIP DNA, as well as input DNA, for sequencing library construction and then sequencing. Please see below for sample submission guidelines.

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Low Input/Single Cell RNA Sequencing

Starting at $450.00 per sample

Requires 15 -150 ng total RNA. Turnaround time is around one month. For users not affiliated with Northwestern/UChicago/UIC, there are two tiers of pricing available, i.e., external academic and industrial. Deliverables are FASTQ files containing Illumina reads passing filter. Bioinformatics data analysis is available upon... Show more »

Requires 15 -150 ng total RNA. Turnaround time is around one month. For users not affiliated with Northwestern/UChicago/UIC, there are two tiers of pricing available, i.e., external academic and industrial. Deliverables are FASTQ files containing Illumina reads passing filter. Bioinformatics data analysis is available upon request. The deliverables include list(s) of differentially expressed genes, and significantly enriched Gene Ontologies and biological pathways.

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Truseq RNA Library Preparation

Starting at $250.00 per sample

TruSeq mRNA-seq, total RNA-seq, and small RNA-seq. Total RNA in the range of 150 ng -1.5 ug is required. For single cell or low input RNA analysis, please check our other RNA-seq services. Turnaround time is around a month. For users not affiliated with Northwestern/UChicago/UIC, there are two tiers of pricing available, i.e.,... Show more »

TruSeq mRNA-seq, total RNA-seq, and small RNA-seq. Total RNA in the range of 150 ng -1.5 ug is required. For single cell or low input RNA analysis, please check our other RNA-seq services. Turnaround time is around a month. For users not affiliated with Northwestern/UChicago/UIC, there are two tiers of pricing available, i.e., external academic and industrial. Deliverables are FASTQ files containing Illumina reads passing filter. Bioinformatics data analysis is available upon request. The deliverables include list(s) of differentially expressed genes, and significantly enriched Gene Ontologies and biological pathways.

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Affymetrix RNA Microarray

Starting at $375.00 per sample

Process all GeneChips offered by Affymetrix.

Process all GeneChips offered by Affymetrix.

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Affymetrix Microarray

Starting at $375.00 per sample

Process all GeneChips offered by Affymetrix.

Process all GeneChips offered by Affymetrix.

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Illumina DNA Microarray

Starting at $41.50 per sample

Process all BeadChips offered by Illumina.

Process all BeadChips offered by Illumina.

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Illumina RNA Microarray

Starting at $115.00 per sample

Illumina HT-12 BeadChips. Turnaround time: 2 weeks.

Illumina HT-12 BeadChips. Turnaround time: 2 weeks.

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Global Methylation Analysis

Starting at $45.00 per sample

Illumina Infinium MethylationEPIC BeadChip processing.

Illumina Infinium MethylationEPIC BeadChip processing.

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Bioinformatics

Starting at $150.00 per hour
Request a quote for more information about this service.

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Cell Line Authentication

Starting at $16.00 per sample
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DNA Extraction

Starting at $19.20 per sample

Automated DNA extraction using robotic workstations. Offers genomic DNA extraction from whole blood, buffy coat, buccal swab, and saliva. The extracted high quality and large molecular weight genomic DNA is suitable for downstream analyses such as genotyping, PCR, next-gen sequencing, microarray, or archival storage.

Automated DNA extraction using robotic workstations. Offers genomic DNA extraction from whole blood, buffy coat, buccal swab, and saliva. The extracted high quality and large molecular weight genomic DNA is suitable for downstream analyses such as genotyping, PCR, next-gen sequencing, microarray, or archival storage.

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Protein-DNA Interaction Analysis

Price on request

Protein-DNA Interaction Analysis Services

Protein-DNA Interaction Analysis Services

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Biomolecular Interaction Analysis

Price on request

Biomolecular Interaction Analysis Services

Biomolecular Interaction Analysis Services

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RNA Microarray Platforms

Price on request
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Biology

Price on request
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Cell Lines

Price on request
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Epigenetic Studies

Price on request
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Cells and Tissues

Price on request
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RNA Microarray

Price on request
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Next Generation Sequencing (NGS)

Price on request
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DNA Microarrays

Price on request
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Project Management

Price on request
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RNA Services

Price on request
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RNA Sequencing

Price on request
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Computational Modeling

Price on request
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Biochemistry & Molecular Biology

Price on request
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DNA Sequencing

Price on request
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Biostatistics & Bioinformatics

Price on request
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Nucleic Acid Services

Price on request
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DNA Extraction and Purification

Price on request
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DNA Services

Price on request
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DNA Library Preparation

Price on request
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