Novogene is a leading provider of genomic services and solutions with cutting edge NGS and bioinformatics expertise and the largest sequencing capacity in the world. Novogene utilizes scientific excellence, a commitment to customer service and unsurpassed data quality to help our clients realize their research goals in the rapidly evolving world of genomics. The company has become a world-leader in NGS services, with 1,800 employees and multiple locations across the globe. Novogene’s depth of experience has resulted in the ownership of 49 NGS-related patents, as well as the publishing of over 1850 customer research papers, often in well-respected publications such as Nature and Science.
Human whole genome sequencing enables researchers to catalog the genetic constitution of individuals and capture all the variants present in a single assay. It is applied to the study of cancer and a variety of diseases, as well as human population evolution studies and pharmacogenomics.
Equipped with 30 powerful Illumina HiSeq X and 25 NovaSeq 6000 systems, Novogene is capable of sequencing up to 280,000 human genomes per year at the lowest cost per genome. Being one of the first few companies to adopt HiSeq X Ten since early 2014, we have extensive experience providing whole genome sequencing services, having successfully sequenced hundreds of thousands of genomes with high quality results. With the world’s largest sequencing capacity, deep experience in whole genome sequencing, and advanced bioinformatics capabilities, Novogene is able to expertly meet customer needs for executing large projects with timely turnaround and the highest quality results.
Exome sequencing provides a cost-effective alternative to whole genome sequencing, as it targets only the protein coding region of the human genome responsible for a majority of known disease-related variants. Whether you are conducting studies in rare Mendelian disorders, complex disease, cancer research, or human population studies, Novogene’s comprehensive human whole exome sequencing service provides a high-quality, affordable, and convenient solution.
Novogene’s bioinformatics analysis includes data QC, mapping with reference genome, SNP/InDel, somatic SNP/InDel calling, statistics, and annotation. Novogene utilizes internationally recognized software in bioinformatics analysis, e.g. BWA, SAMtools, GATK, etc.
In particular, Novogene's bioinformatics pipeline includes annotation with the exome aggregation consortium (ExAC). ExAC dataset spans 60,706 unrelated individuals sequenced as part of various disease-specific and population genetic studies. This population scale database greatly facilitates research of disease pathogenesis.
The Novogene Advantage
Compared with microarray analysis, mRNA sequencing (RNA-Seq) provides a more precise and complete snapshot of the transcriptome and enables the identification of novel transcripts, alternative splicing, and gene fusion events. RNA-Seq also provides an alternative and affordable approach for gene expression quantification and differential gene expression analysis among groups of samples. It is widely used in disease research, drug response research, pharmacokinetics, and personalized healthcare research.
Novogene offers complete solutions for transcriptome sequencing and RNA-Seq quantification using state-of-the-art Illumina NovaSeq and HiSeq platforms with paired-end 150 bp (PE 150) sequencing strategy. The longer read length ensures superior data quality and accurate sequence assembly. Our experienced bioinformaticians work closely with customers to provide standard and customized data analysis and publication ready results for species with and without a reference genome.
Small RNAs are a type of non-coding RNA of 50-250 nucleotides in length that regulate the translation of target mRNAs. Small RNA sequencing, including the sequencing of microRNA (miRNA-Seq), is a valuable method for investigating the function of small RNAs and for constructing regulatory networks of miRNAs and their target genes.
Novogene offers comprehensive small RNA sequencing and bioinformatics analysis for miRNA and other types of small RNAs, including siRNA and piRNA. Scientists at Novogene use the cutting-edge Illumina platform, with the SE50 sequencing strategy and widely accepted analysis software, to support RNA research on a wide range of biological systems. Please contact us for more information.
Accurately characterizing the diverse landscape of transcript isoforms generates key information for understanding the functional biology of genomes, but building complete transcripts, has been challenging. Isoform sequencing (Iso-Seq™), based on the extraordinarily long reads available with PacBio SMRT technology, provides full-length reads spanning entire transcript isoforms from the polyA-tail to the 5′ end, with no assembly needed! Iso-Seq is ideal for improving annotations in reference genomes, for characterizing gene isoforms, alternative splicing and gene fusion events, as well as for complementing the short/incomplete transcripts for species without a reference genome.
With the largest Illumina and SMRT sequencing capacity in the world, Novogene offers complete solutions for full-length transcript sequencing using combined Illumina and PacBio Sequel platforms. Illumina short reads are further mapped to the full-length transcripts generated from Sequel for error correction and for more accurate quantification analysis. Our experienced bioinformatics experts work closely with customers to provide standard and customized data analysis and publication-ready results for species with and without a reference genome.
DNA methylation at the C5 position of cytosine plays a crucial role in gene expression and chromatin remodeling, and perturbations in methylation patterns are implicated in the development of cancer, neurodegenerative diseases, and neurological disorders. Therefore, the mapping of methylated bases (the methylome) is critical to understanding gene expression and other processes subject to epigenetic regulation.
Novogene provides whole genome sequencing of bisulfite-converted DNA as an effective method to identify individually methylated cytosines on a genome-wide scale. Methylome analysis is an increasingly valuable research tool with a range of applications, including studies on gene regulation, stem cell differentiation, embryogenesis, aging, cancer and other diseases, and phenotypic diversity and evolution in plants and animals.
ChIP-Seq provides genome-wide profiling of DNA targets for histone modification, transcription factors, and other DNA-associated proteins. It combines the selectivity of chromatin immunoprecipitation (ChIP) for recovering specific protein-DNA complexes with the power of NGS for high-throughput sequencing of the recovered DNA. Additionally, because the protein-DNA complexes are recovered from living cells, binding sites can be compared in different cell types or tissues, or under different conditions.
In ChIP-Seq, enriched DNA regions (protein binding sites) are detected as peaks above background reads, and bioinformatics analyses of these regions can reveal binding motifs. Applications include studies on gene regulation, transcription complex assembly, histone modification, developmental mechanisms, and disease processes. At Novogene, we can provide you with high quality sequencing and comprehensive bioinformatics analysis for your ChIP-Seq project.
Advances in whole-genome amplification and Novogene’s expertise have made single-cell sequencing easily accessible to researchers. Novogene is one of the few NGS providers with extensive experience in single-cell sequencing technology, including single-cell DNA sequencing. We offer the highest quality services in amplification, library construction, sequencing, and bioinformatics analysis to our customers, and our results have been published in leading scientific journals.
With single-cell DNA sequencing, the genomic heterogeneity of cell populations can be explored at the level of the individual cell. Genetic changes, such as point mutations and copy number variation occurring during disease and normal development processes, are profiled using the minute amounts of DNA from single cells. Applications include analysis of genetic heterogeneity within unicellular and multicellular organisms, detection of chromosomal anomalies in germ line cells, preimplantation genomic screening of embryos, and defining the genetic composition of tumors for developing more targeted therapies.
Single-cell sequencing is now readily available to researchers through Novogene’s expertise in this field. Novogene is one of the few NGS providers with extensive experience in single-cell sequencing technology, including single-cell RNA-Seq. We offer the highest quality services in amplification, library construction, sequencing, and bioinformatics analysis to our customers, and our results have been published in leading scientific journals.
Single-cell RNA-Seq enables the high-resolution transcriptome profiling of a single cell, and has broad utility for investigating developmental processes and gene regulatory networks, and for revealing heterogeneous gene expression patterns within cell cultures, tissues, and organs. Applications include profiling gene expression changes during stem cell differentiation, organ development, or tumor progression, and characterizing the response of subpopulations of cells to environmental signals and conditions.
Novogene offers the most advanced techniques for transcriptome analyses for single cells.
Using de novo sequencing to obtain the genomic information of microbes provides a fresh start for exploring the genetic structure and functions, studying the evolutionary origin of microbial populations, as well as developing potential applications of these abundant microbes in medicine, disease, agriculture, and environment.
Novogene is at the forefront of de novo sequencing as it becomes more rapid and affordable. Novogene’s founder, Dr. Ruiqiang Li, is a leading genomics expert and a primary developer of the SOAPdenovo software package for genome assembly. Dr. Li and the Novogene team have contributed to many important publications on novel genome sequences, and we can provide you with the high level of expertise required for your specific project.
For the microbial genome, Novogene offers de novo sequencing service using both PacBio and Illumina platforms. We provide multifaceted sequencing services including genome survey, frame map, complete map, and fine map tailored to different research needs. For each project, our scientists will design the best sequencing strategy utilizing an optimal combination of short reads and long-range sequencing information to achieve the most comprehensive de novo assembly results for your genome of interest.
Amplicon sequencingAmplicon Sequencing is frequently used to identify and differentiate microbial species. Short (<500 bp) hypervariable regions of conserved genes or intergenic regions are amplified by PCR and analyzed using NGS technology, and the resulting sequences are compared against microbial databases.
For bacteria and archaea, the 16S rRNA gene is the most common target for amplicon sequencing. For fungi, three targets are generally used: the 18S rRNA gene and two internal transcribed spacers (ITS) located between rRNA genes. These regions are usually sufficiently divergent to separate even highly related species, and can sometimes differentiate subspecies.
At Novogene, we have sequenced over 170,000 microbial samples for our customers. Our standard bioinformatics analyses include alpha-diversity analysis, OTU analysis, species annotation, beta-diversity analysis, and multi-variate statistical analysis. Applications range from identifying a single species in pure culture to characterizing the microbiota of animals or plants, to comparing species diversity and population structure in various environmental sources or geographic regions. Our specialists can advise you on the appropriate analyses for your project.
In metagenomics, genomes from environmental samples are analyzed without the prior isolation and cultivation of individual species; therefore, it is a powerful technique for studying microbial communities in their natural habitat, with a broad range of applications. At Novogene, our customers can rely on our expertise in NGS to help them explore the rich genetic repertoire of microbial communities, while also benefiting from our bioinformatics expertise to help identify the species, genes, and pathways represented in their samples. Novogene provides metagenomic sequencing service with Illumina HiSeq platform and assembly-first strategy, and our bioinformatics analyses provide gene predictions, function annotations, and taxonomic annotations. Our standard analysis package includes mPATH, heatmaps, PCA, cluster analysis, and other programs, generating high-quality, publication-ready data.
Next Generation Sequencing (NGS) Services
Biochemistry & Molecular Biology Services
Chromatin Immunoprecipitation (ChIP) Assays Services
Protein-DNA Interaction Analysis Services
Biomolecular Interaction Analysis Services
Novogene(HK) Company Limited has not received any endorsements.