Novatein Biosciences is a rapidly growing biological reagents company, specializes in the production and supply of immunoassay kits, reagents and proteins to the researchers worldwide. We supply more than 10,000 various types of proteins and antibodies, and have one of the richest supplies of validated immunoassay kits.
In addition, we function as a contract research organization (CRO) assisting our clients in protein/ antibody production and immunoassay development and setup. As a manufacturer of reliable ELISA kits, our scientists are experts in performing and troubleshooting different types of sandwich assays including ELISAs. Our laboratory in Woburn, MA is equipped with BSL2 cabinet, CO2 incubator, shakers, incubator-shakers, ELISA plate washer, ELISA plate reader, protein spotter, array imager, FRET and alphascreen plate reader, qPCR cycler, gel documentation system, freezers, refrigerators, fume hoods, centrifuges, pH meter, western blot apparatus, PCR machine, FPLC/HPLC instrument, microscope etc. Our mission is to accelerate R&D by providing high quality reagents and timely services.
With decades of experience, we are experts in creating and optimizing stable and transient expression for secreted or intracellular, cytosolic or periplasmic, tagged or untagged proteins. We assess expression levels using the SDS-PAGE, Western, ELISA or functional analysis. We optimize protein expression by modification of, promoter, ribosome binding site, vector copy number, host cell, growth condition including media and additives, time, and temperature.
In addition, we offer the following services:
Inclusion body purification
Refolding with typical or preferred protocols.
Proteins are purified and formulated with highest purity, solubility, and most active form.
Note: For most of the eukaryotic protein production, baculovirus expression system is the first line of choice due to high yield and posttranslational modifications. If the protein activity is dependent on the posttranslational modifications, the mammalian expression system is the choice.
• Ph.D. level scientists experienced in customer support
• Wave-bag and shaking flask for small to medium scale protein production
• AktaXpress, Akta Avant, various columns for purification using affinity, IEX, SEC and combined methods.
• Endotoxin detection and removal
• Protein QC with SDS-PAGE, analytic SEC, etc., imaging with Chemidoc XRS
Mammalian cells: CHO, HEK293 and others (5 – 7 weeks)
With years of experience, we are experts in creating and optimizing stable and transient expression for secreted or intracellular, cytosolic or periplasmic, tagged or untagged proteins. We assess expression levels using the SDS-PAGE, Western, ELISA or functional analysis. We optimize protein expression by modification of, promoter, ribosome binding site, vector copy number, host cell, growth condition including media and additives, time, and temperature
Schematic for Baculovirus Expression:
Gene of interest (GOI) is cloned into a suitable donor plasmid containing a mini-Tn7 element.
The recombinant donor plasmid is then transformed into E. coli cells which contain a bacmid with a mini-attTn7 target site and a helper plasmid. The mini-Tn7 element on the donor plasmid facilitates transposition of the GOI into the target site on the bacmid resulting in insertion of the GOI into the bacmid DNA.
Recombinant bacmid DNA is extracted and purified (for efficient homologous recombination, pure DNA is required).
Insect cells are transfected with the recombinant bacmid DNA to produce recombinant baculovirus particles with GOI (P1 virus generation).
The infectious potency of P1 baculovirus is determined by plaque assay (site specific transposition does not require P1 viral particles purification)
The insect cell cultures are infected with the purified P1 stock to create a P2 stock (100 ml serum free medium).
A high quality high titer working virus stock P3 (~500 ml) is produced by infecting Sf9 cells with a P2 virus at a low multiplicity of infection (MOI=0.1)
Optimization of heterologous protein expression and purification of protein by successive chromatography techniques
Typical turn around time: Depends on the complexity of the project. (Expect the fastest turn around time).
Small to large volume antibody production with low endotoxin level (< 1 EU/mg).
Transient transfection with ExpiCHO or Expi293 cells.
Quick turn around time from cloning, expression testing, to purification.
We offer reliable antibody sequencing, cloning and expression/purification services. With your hybridoma cell line pellet ( 1 x 10^7), we will sequence heavy and light chain variable regions, variable regions plus leader sequences, or full-length heavy and light chain antibodies.
Furthermore, we offer antibody production services, including cloning of heavy and light chain variable regions or full-length antibodies, co-transfection and expression, purification and validation of antigen binding by plate based assay.
• Total RNA extraction from hybidoma or B cells.
• cDNA synthesis from mRNA by reverse transcription.
• 5’ RACE PCR to get DNA fragments corresponding to VH and VL.
• Cloning into a sequencing vectors like pUC118 and sequencing.
• Sequencing analysis ( sequence data, alignment of each chain) for at least 5-10 independent clones: both VH and VL
• Sequencing data summary report, delivery of plasmid.
Variable Domains Sequencing for both Light chain and Heavy chain:
$950, 2 weeks.
Variable domains sequencing for both LV and HV, plus leader sequence.
Cat# CS8-2: $1200, 2 - 3 weeks
Full Length sequencing for variable regions, constant domains and leader sequences:
$1400, 3 weeks
Screening sandwich assay pairs for antibody-antibody, antibody-aptamer, aptamer-aptamer combination in 2-4 weeks based on project requirement.
Establish and validate immunoassay kit for both sandwich and competitive methodology.
Antibody conjugation, protein and small molecule antigen conjuation.
Assay in TR-FRET, AlphaLISA, ELISA, MSD, immuno-PCR format
We will use either user-defined method or develop a new method for the analysis. Our company has developed a powerful non-Luminex, non-microarray platform to handle biomarker panels with analytes number >500.
Alpha technology (Amplified Luminescent Proximity Homogeneous Assay) is a homogenous, no-wash assay method used to study biomolecular interactions in microplate format. The technology is based on Donor and Acceptor beads containing a proprietary mixture of chemicals, which, when excited emit luminescent signal. The Donor bead contains phthalocyanine which converts ambient oxygen (O2) to singlet oxygen when excited at 680 nm. As the singlet oxygen has a very short half-life (4 µsec) it is able to diffuse 200 nm in solution and transfer its energy to the thioxene derivatives within the acceptor beads resulting in luminescent signal production at 520-620 nm (AlphaScreen) or 615 nm (AlphaLISA). If there is no acceptor bead present within 200 nm in solution, singlet oxygen falls back to the ground state and no signal is produced.
Biomarker/ cytokine detection: Immunoassays of secreted analytes from cell Culture or serum/ plasma
Cell signaling- To detect epigenetic/ kinase post-translational modifications
Cell-based assays- For studying pathways in whole cells, or studying endogenous receptors
To study protein—protein and protein—nucleic acid interactions ¬
Second messengers: e.g., GPCR research
Ligand-Receptor Interactions Detection of Epigenetic or Post-Translational Modifications
Examples of Projects Completed at Novatein Biosciences
Typical turnaround time: 5-7 business days
As a manufacturer of reliable ELISA kits, our scientists are experts in performing and troubleshooting different types of ELISAs. We use good laboratory practices, to perform our assays either for assay validation or quality control.
Customers have the flexibility to choose our in-house manufactured ELISA kit, in which case we offer 10%-25% discount on the kit price.
Number of samples processed in a single experiment: 100
Typical turn-around time: 3-5 business days depending on the sample volume (for the kit in stock or provided by the customer)
$200 per 96-well plate (if the kit is purchased from us)
$250 per 96-well plate (if the kit is purchased from outside)
1 96-well plate minimum (multiples of 96 well plates)
Immuno-PCR (iPCR) is a method that combines the advantages of both enzyme-linked immunosorbent assay (ELISA) and PCR, is a powerful method for detecting low quantities of protein antigens. Despite its potential, for a long time iPCR was an underutilized method as evidenced by the low number of publications on its routine application. The introduction of ready-to-use reagents, reduction of protocol time and the development of reliable systems is opening the way for iPCR to become a routine method for use as a way for sensitive antigen/antibody detection in clinical setting, as well as a more popular tool to study the protein target in drug discovery research. Its advantage over traditional ELISA includes but not limits to:
A typical 100–10 000-fold improvement over the detection limit of the ELISA has been obtained in almost all applications.
For medium level targets, a lower than 1 uL of serum, plasma, urine, CSF, supernatant, or 1 ug cell lysate is enough for the detection assay. This will greatly save your precious samples.
Due to low sample volume or high dilution, the matrix effect originated from serum, plasma, CSF, cell lysate or others will disappear or be minimized.
Detection for low abundant targets becomes reality. Since the detection limit is extended from 1- 100 ng/mL to 1 -100 pg/mL, or from to 1 -100 pg/mL to 1 -100 fg/mL, the changes of many biomarkers can be detected for drug efficacy or patient status evaluation.
Novateinbio will deliver the best in-house assay development for your ultrasensitive target detection. In the meantime, we can also deliver validated ultrasensitive kits to you at a reasonable cost and fast turn-around time. Please contact us for details.
We offer a full range of qPCR services for gene expression analysis.
qPCR primer/ probe design
Taqman based assays
SYBR Green based assays
Ph.D. level scientists- experienced in customer support
Agilent Mx3005P qPCR system
Centrifuges/ pipettes/ spectrophotometer
Typical turn-around time: 3-5 business days depending on the sample volume
$8-10 per well/ gene (minimum 96-well plate)
Less than $10 if multiplexing
express and purify protein from E.coli, HEK293/CHO cells from 100 ug to 1000 mg level.
As a manufacturer of reliable antibodies, our scientists are experts in performing and troubleshooting different types of antibody related assays. We use good laboratory practices, to perform our assays either for validation or quality control.
Customers have the flexibility to choose our in-house manufactured antibodies, in which case we offer minimum 10% discount on the antibody price.
We provide plasmid DNA purification service at various scales (mini, midi and maxi). Ask for endotoxin removal, if needed.
Send the samples as bacterial pellet or plasmid DNA (to be amplified) itself , we will perform bacterial transformation and plasmid isolation.
Transformation and mini-prep: $75
Price is dependent on the number of samples.
We provide complete PCR service including primer design, reaction optimization and purification.
We also perform PCR for genotyping.
We provide buffers/ solutions/ common reagents in bulk quantities.
We offer plate coating service for ELISA
"The work Dr. Reddy has done is beautiful. Dr. Reddy has been very responsive and very through in the assay development. We will definitely work with the team again."
A couple of months ago, i had a project in hand which needed lots of Protein purification, antibody purification and optimization. I was expecting them to react in a week or two. But to my surprise, they emailed me within a day. They made a very well organized and reasonable quote for the project. Looking at the results and the way they handled it, i am totally satisfied. Not a single dollar i spent was wasted. I am very much happy for finding this company. Their problem solving approach is totally commendable.
I'm happy with Novatein Biosciences so far. Their product arrived on time and was high quality. Also, they have wonderful customer service and I have personally spoken with members of their team for my requests. They seem to be a small company but they are definitely doing good work there. My experiences with Novatein Bio are as expected from a professional provider and I look forward to working with them in the future!