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NovAliX

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NovAliX provides small molecule drug discovery services; serving pharmaceutical companies via a unique combination of screening, medicinal chemistry & structural biology. Clients from all over the world, in key industrial sectors, rely upon the laboratories of NovAliX to deliver the innovations that future business growth depends upon through the successful completion of challenging projects.

Based upon the initial concept of transferring academic knowledge into valuable industrial applications; NovAliX has become a trusted provider of expert services to clients ranging from virtual companies to the global players. NovAliX provides a valuable extension to any in-house capabilities ensuring that managers, scientists and project leaders meet or exceed their targeted objectives.

NovAliX has not listed any services.

Fragment Screening
Price on request

Traditional high-throughput screening campaigns use high numbers of large and complex compounds. Typically there are a low percentage of hits exhibiting strong affinities to a target protein. Unfortunately, in some cases the hit-to-lead process is hindered by the high complexity of the hits leading to overly complex molecules with... Show more »

Traditional high-throughput screening campaigns use high numbers of large and complex compounds. Typically there are a low percentage of hits exhibiting strong affinities to a target protein. Unfortunately, in some cases the hit-to-lead process is hindered by the high complexity of the hits leading to overly complex molecules with sub-optimal druggability properties.

FBDD starts with simpler lower molecular weight compounds referred to as fragments. Binding is weak but makes efficient use of the molecular structure. FBDD screens therefore require high sensitivity. NovAliX has set up a state of art biophysical platform, which consists of chemical microarray SPR, native MS and NMR spectroscopy. Fragment hits are evolved or linked according to modern rational design principles. This generates novel molecules with high binding efficiency, high affinity & high selectivity for the target protein together with optimal drug-like properties.

NovAliX has become a provider of the key state-of the art capabilities integrating biophysical techniques, including chemical microarray SPR, native MS, X-ray crystallography, NMR spectroscopy and ITC, significantly enhanced by expertize in library design, medicinal chemistry and CADD.

The decision to choose NovAliX to support your FBDD project predestines the achievement of your project goals.

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Affinity Chromatography
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Hundreds of therapeutic proteins are currently in clinical development and on the market. These complex, heterogeneous protein-based products are bio-reaction products that are purified using multiple filtration and chromatographic steps. Affinity chromatography using protein A is first step of most technology platforms for the... Show more »

Hundreds of therapeutic proteins are currently in clinical development and on the market. These complex, heterogeneous protein-based products are bio-reaction products that are purified using multiple filtration and chromatographic steps. Affinity chromatography using protein A is first step of most technology platforms for the purification of some classes of monoclonal antibody. However protein A affinity is not universally applicable to all proteins.

NovAliX offers a unique platform for rapid discovery and development of customized synthetic affinity ligands for cost-effective purification of proteins. The components of this platform are: 1) NovAliX’ proprietary chemical microarray SPR screen; 2) the diverse NovAliB library of immobilized small molecules; and 3) NovAliX’ extensive synthetic-chemical and biophysical services (e.g. Hit progression chemistry and protein crystallography). Potentially useful ligands are characterized against a number of chromatographic performance parameters. The ligands thereby discovered are specific binders, with high chemical stability and submicromolar to lower micromolar affinities; ideal for purification or chromatographic applications. Such small molecule affinity ligands are economical to produce. The discovery process itself establishes the site for attachment of the linker. A functionalization at this given attachment point allows functionalizing the ligands and facilitates immobilization to the preferred chromatographic support.

Workflow

The target protein is screened against the entire NovAliB library via the NovAliX chemical microarray SPR platform. Additionally, a counter-screen with potential feed impurities is performed to verify the required specificity. Hits are selected for immobilization for example on agarose for chromatographic characterization. Miniaturized microtiter plate assays rapidly determine binding behavior, selectivity, isotherms, kinetics and chemical stability of the hit structures. Dynamic binding capacity and preparative column chromatography are investigated. Affinity ligands performance can be further optimized by employing the NovAliX expertise more commonly used to support drug discovery programs; hit to lead and lead optimization and SBDD.

Features

  • Proprietary screening approach for the discovery of high quality affinity ligands
  • Rapid results – within 6 months for full chromatographic characterization of screening hits
  • Universally applicable to all soluble proteins
  • High specificity for defined binding sites can be developed
  • Development of target specific and group specific (e.g Fab) binders possible
  • Screening of immobilized molecules facilitates transfer to chromatography format
  • Fine-tuning of performance is a clearly defined process
  • Variation of linker for the coupling to the chromatographic support matrix of choice
  • Novel affinity systems are characterized by chromatographic performance
  • Leverages expertise developed for medicinal chemistry and SBDD
  • Evaluation and optimization of the best chemical routes

Benefits

  • High probability of success – applies established paradigms
  • High novelty content – strong intellectual property protection – patentable
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Medicinal Chemistry
Price on request

NovAliX provides access to integrated medicinal chemistry services conforming to the highest industrial standards. The state of the art drug discovery processes range from primary screening to the delivery of optimized leads for development via hit to lead (H2L) and lead optimization (LO) programs.

The combination of scientific... Show more »

NovAliX provides access to integrated medicinal chemistry services conforming to the highest industrial standards. The state of the art drug discovery processes range from primary screening to the delivery of optimized leads for development via hit to lead (H2L) and lead optimization (LO) programs.

The combination of scientific knowledge, a broadly based biophysical platform and numerous innovations allows NovAliX to tackle the most challenging discovery projects flexibly, rapidly and cost-effectively. Our expert medicinal chemists steer projects though each phase of the drug discovery process from hit generation, through lead generation to lead optimization.

NovAliX offers invaluable starting points even for the most challenging projects thanks to our proprietary small molecule libraries and chemical micro-array SPR technology. Hits are confirmed via an integrated process encompassing native MS, X-ray crystallography and computer aided drug discovery (CADD). The medicinal chemists strive hard to minimize late-stage attrition of development candidates. Lead generation and LO take account of multiple factors; potency, selectivity, solubility, permeability and metabolic stability in order to identify the most promising development candidates.

Whilst the cost of pharmaceutical research and development is of great concern, in the discovery process merely focusing on budgetary control is insufficient. Investing in skilled experts generating accurate data give the best chance of reaching a successful outcome. Assessments by the NovAliX team rigorously minimize scientific errors in recommending candidates to enter clinical development.

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Hit Identification
Price on request

NovAliX’ diverse and complementary screening technologies are the foundation of a powerful hit generation platform, both in the fragment and lead-like chemical spaces. Chemical microarray SPR, which is our main screening technology, is conducted against a given target using the NovAliB library.

Chemical microarray SPR imaging... Show more »

NovAliX’ diverse and complementary screening technologies are the foundation of a powerful hit generation platform, both in the fragment and lead-like chemical spaces. Chemical microarray SPR, which is our main screening technology, is conducted against a given target using the NovAliB library.

Chemical microarray SPR imaging enables screening in a medium to high throughput fashion. The quality of the data is highest when crystallographic grade protein is used. NovAliX has in-house protein expression and purification facilities.

Next, screening conditions are systematically optimized to identify real hits and to minimize artifacts. The most promising hits for hit to lead progression are determined by use of computer assisted algorithms and expert selection. NovAliX has several workflows for biophysical and in silico screening. Innovations include a proprietary chemistry oriented search engine that is more effective than other tools at interrogating chemical databases making best use of prior knowledge.

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Library Design and Synthesis
Price on request

NovAliX is a highly discovery oriented organization with considerable knowledge of strategies for the design and synthesis of tailor-made libraries for pharmaceutical and biotechnology clients.

The large and strong discovery chemistry team is of considerable assistance to clients that want to expand and diversify a library of... Show more »

NovAliX is a highly discovery oriented organization with considerable knowledge of strategies for the design and synthesis of tailor-made libraries for pharmaceutical and biotechnology clients.

The large and strong discovery chemistry team is of considerable assistance to clients that want to expand and diversify a library of compounds, leveraging the full chemical potential of any scaffolds to design exclusive and useful additions to the collection.

Our target-oriented approach provides exclusive screening libraries that are focused towards any target for drug development.

Exclusive library development has a typical workflow:

  • Development of robust synthetic pathways to access customized building blocks and scaffolds
  • Chemistry or target-driven exploitation of the scaffolds’ full potential
  • Verification studies in preparation for parallel synthesis of the library
  • Parallel synthesis on a multi-milligram scale yielding libraries of 10-1000 members

The most appropriate input and techniques are carefully selected on an individual basis for each project. Library synthesis may utilize solution phase, solid phase or polymer assisted solution phase reactions.

The latest techniques are used for rapid or parallel purification such as solid phase extraction, solid supported liquid-liquid extraction & polymer supported quench and preparative LC with MS detection.

Quality control from the analytical department ensures that all compounds fully meet the required specifications.

Exclusive library development is remunerated on a FTE basis.

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Compound Synthesis
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NovAliX provides reliable re-synthesis of standards & references for research & development. Clients include pharmaceutical, cosmetic, agrochemical and fine chemical companies, contract research organisations, universities and research institutions.

The team can re-synthesize and verify the quality of any chemical... Show more »

NovAliX provides reliable re-synthesis of standards & references for research & development. Clients include pharmaceutical, cosmetic, agrochemical and fine chemical companies, contract research organisations, universities and research institutions.

The team can re-synthesize and verify the quality of any chemical substance in compliance with your specifications.

  • Analytical standards
  • Reference compounds
  • Final target compounds
  • Advanced intermediates
  • Metabolites & impurities
  • PET precursors & standards
  • Combinatorial scaffolds & building blocks
  • Biochemical tools, fluorescent probes & click chemistry

NovAliX compound re-synthesis delivers high quality for a highly competitive fixed price.

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Process and Scale-Up Chemistry
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Innovative scaleable routes for biologically active substances, intermediates & specialty chemicals

The ultimate performance of a chemical manufacture is predetermined by the synthetic route itself. Despite the high probability that a new drug will fail in development, cutting corners by proceeding with a sub-optimal... Show more »

Innovative scaleable routes for biologically active substances, intermediates & specialty chemicals

The ultimate performance of a chemical manufacture is predetermined by the synthetic route itself. Despite the high probability that a new drug will fail in development, cutting corners by proceeding with a sub-optimal process all too often proves costly sooner or later. The systematic creativity of NovAliX chemists transforms complicated lab-scale medicinal chemistry routes into shorter scalable manufacturing processes. Before long the small investment pays for itself many times over, even on the sub-kilogram scale.

Conversely, in the case of mature commercial products, new chemistries raise the threat that competitors will gain advantage through superior technology. NovAliX has a unique way of integrating the experience and creativity of medicinal chemists with the additional discipline of the process chemist. This leads to superior routes that are systematically verified to achieve specific objectives. Contributions include, IP protection, rapid implementation, next generation or breakthrough processes, debottlenecking as well as reduced capital investment and a lower cost of goods.

Whether your process R&D challenge is opportunistic or protective; NovAliX has the right expertize. The innovative NovAliX process R&D platform is engineered to deliver correspondingly innovative solutions.

Offers Deliverables
Paper route design & selection Identification of routes
Process research & route scouting Synthesis lock
Individual step optimization Condition optimization
Full process development Process lock
Scale-up & manufacturing Demo batch supply & production

Projects vary enormously in size and scope and timescale so are executed on a FTE basis against an agreed plan. Backed by over 10 years of operational experience in conducting external collaborations, NovAliX executes projects efficiently, delivers accurate reports and fully supports technology transfer. Throughout the relationship the highest professional industrial management practices are maintained.

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Differential Scanning Calorimetry
Price on request

In addition to the SPR, NMR and native-MS expertize, NovALiX routinely perform other important orthogonal techniques for the characterization of protein ligand association. These are principally isothermal titration calorimetry (ITC) and differential scanning calorimetry (DSC) in order to strengthen the data set relating to any... Show more »

In addition to the SPR, NMR and native-MS expertize, NovALiX routinely perform other important orthogonal techniques for the characterization of protein ligand association. These are principally isothermal titration calorimetry (ITC) and differential scanning calorimetry (DSC) in order to strengthen the data set relating to any set validated binders to a target.

Differential scanning calorimetry

DSC is able to show shifts of protein melting temperature (Tm) that occurs upon ligand binding. DSC quickly establishes if ligand binding to a protein gives a stabilized complex. Unlike ITC, DSC may enable correlation of the thermodynamics that drive binding with conformational changes in the macromolecule caused by the binding reaction. DSC is particularly useful in characterizing very tight binding interactions.

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Isothermal Titration Calorimetry (ITC)
Price on request

In addition to the SPR, NMR and native-MS expertize, NovALiX routinely perform other important orthogonal techniques for the characterization of protein ligand association. These are principally isothermal titration calorimetry (ITC) and differential scanning calorimetry (DSC) in order to strengthen the data set relating to any... Show more »

In addition to the SPR, NMR and native-MS expertize, NovALiX routinely perform other important orthogonal techniques for the characterization of protein ligand association. These are principally isothermal titration calorimetry (ITC) and differential scanning calorimetry (DSC) in order to strengthen the data set relating to any set validated binders to a target.

Isothermal titration calorimetry

ITC tends to be used as a secondary or orthogonal technique providing real-time label-free direct measurements binding constant (kD), stoichiometry (n) of binding and thermodynamic parameters of interactions in solution. ITC is unique in being able to measure changes in the two thermodynamic parameters the enthalpy (ΔH) and entropy (ΔS) that define the binding affinity.

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ProteoPlex MacroDSF
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Structure Determination
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NovAliX structural biology group leads the world in nuclear hormone receptors. This expertise has been applied to numerous protein families including kinases, proteases, phosphatases, epigenetic targets, phosphodiesterases and protein/antibody complexes.

The highly effective protein production and purification and... Show more »

NovAliX structural biology group leads the world in nuclear hormone receptors. This expertise has been applied to numerous protein families including kinases, proteases, phosphatases, epigenetic targets, phosphodiesterases and protein/antibody complexes.

The highly effective protein production and purification and crystallization platform underpin the effectiveness of X-ray structural determinations. Projects range from structure determination of targets already in our crystal gallery to reproduction of published data and de novo structure determination. The majority of structural determinations are of co-crystals with small molecule ligands to support rational drug design approaches to drug discovery.

Crystal gallery

The NovAliX crystal gallery provides a wide choice of targets. Amongst the more than 80 targets, there are a large number of protein families including nuclear hormone receptors, kinases and epigenetic targets. In just 4 – 12 weeks we routinely determine co-crystal structures with novel ligands selected by our clients.

Reproduction of published data

For non-gallery proteins, where prior art exists, structures are obtained within 6 – 14 weeks.

De novo structures

De novo structural determination is usually achieved by designing multiple constructs after extensive sequence analysis with bioinformatics tools. The soluble constructs are used to generate crystals. Using SAD/MAD methods or molecular replacement to solve the phase problem the crystallographers usually achieve structural determinations within 3 – 6 months.

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Protein Production
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The protein factory produces crystallographic grade recombinant protein for investigations using the wide range of NovAliX biophysical techniques: NMR, SPR, ITC, and MS. In addition proteins are produced and supplied directly to clients. De novo protein, labeled protein and off-the-shelf protein from the protein gallery are... Show more »

The protein factory produces crystallographic grade recombinant protein for investigations using the wide range of NovAliX biophysical techniques: NMR, SPR, ITC, and MS. In addition proteins are produced and supplied directly to clients. De novo protein, labeled protein and off-the-shelf protein from the protein gallery are provided with MS, SDS-PAGE and DLS quality control data.

Off-the-shelf protein production

If a target is already in the protein gallery, the protein is freshly prepared to ensure it is of the highest quality and delivered within 2 weeks. The gallery includes a large number of nuclear receptors, kinases and epigenetics targets. Reproduction of literature data is performed with a quick turn-around time.

De novo protein production

Years of experience in protein production are the basis of a reliable gene to protein service for de novo targets. The probability of success within the shortest timeframe is achieved with multiple constructs designed after extensive sequence analysis supported by bioinformatics. Codon optimized constructs are tested simultaneously in bacteria and baculovirus infected insect cells. Purification produces proteins of the highest quality (identity, purity, integrity and monodispersity). Proteins can be produced with any fusion tag including; His, GST, MBP, TRX, Strep. Proteins are available in quantities of 5 to 50 mg and can be supplied according to client specifications – concentration, buffer, size of aliquot and package.

Labeled-protein production

NovAliX produces labeled proteins: seleno-methionine labeled protein for de novo structural determination and 15N and 13C labeled protein for NMR structural studies.

Protein quality control – leaving nothing to chance

Proteins produced by NovAliX are rigorously purified and characterized for identity, purity, and homogeneity. The principal tools are sodium dodecylsulphate polyacrylamide gel electrophoresis (SDS-PAGE), dynamic light scattering (DLS) and mass spectrometry (MS). SDS-PAGE confirms purity and size. DLS measures any aggregation that may be present.

Arguably the most informative technique is MS. MS analysis of whole proteins under denaturing and native conditions will reveal the presence of any adventitious ligands. Other methods would not reveal this potentially vital piece of information.

If greater characterization is required then MS is again particularly powerful. The presence of post-translational modifications such as glycosylation or phosphorylation can be confirmed by their removal by specific chemical or enzymatic reactions. Peptide mapping, i.e. protein cleavage with proteases may be used to confirm the unique identity of the protein or even to confirm the full sequence and most modifications.

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TEM
Transmission Electron Microscopy
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Transmission Electron Microscopy (TEM) is an established technique to analyze the structure of thin samples. TEM projects the image of protein complex particles, viruses or cells deposited on a thin film or embedded in a thin layer of frozen ice (Cryo-TEM) onto a detector (like a photographic film or a camera). The projections of... Show more »

Transmission Electron Microscopy (TEM) is an established technique to analyze the structure of thin samples. TEM projects the image of protein complex particles, viruses or cells deposited on a thin film or embedded in a thin layer of frozen ice (Cryo-TEM) onto a detector (like a photographic film or a camera). The projections of the objects will be reconstructed in silico to obtain a tridimensional structure. Hence, crystallization is not a prerequisite to determine the structure of macromolecular complexes. Optimal results using TEM can currently be performed on complexes that are 250kDa or larger. However, this limit is being pushed downward by new technology such as direct electron detectors. As TEM enables visualization of a wide range of particles in solution, it complements the biophysical toolbox available from NovAliX.

Negative Staining Transmission Electron Microscopy

Single particle analysis (SPA) using Negative Staining Transmission Electron Microscopy is a powerful technique to characterize the morphological features of protein complexes under specific circumstances (like epitope mapping) at intermediate resolution (20Å and above). The sample is deposited on a thin layer of carbon and surrounded by stain. As this staining is usually made of heavy metal salt, it will provide a very high contrast and is less radiation sensitive than biological matter. The sample preparation and data acquisition can be performed at room temperature and the required concentration is one to two orders of magnitude lower than X-Ray crystallography and NMR, although the target molecules need to have a minimum size. There is no limitation in terms of maximum size. Similar particles can be classified in groups and averaged together to improve the signal to noise ratio. If the protein complexes don’t show a preferred orientation, 3D reconstruction can be performed at intermediate resolution to generate an initial model. Such model is usually the starting structure for a more advanced 3D Cryo-TEM Single Particle Analysis (see below).

3D Cryo-Transmission Electron Microscopy Single Particles Analysis

Three Dimensional Cryo TEM Single Particles Analysis (3D cryo-TEM SPA) has been used to resolve tertiary structures of large proteins and quaternary structures of protein complexes down to the molecular and sometimes near-atomic resolution. Since crystallization or a high concentration of the sample are not mandatory prerequisites to obtain a good structure, Cryo-TEM is applicable to most proteins and provides direct observation of the mechanical dynamics and conformation of flexible structures close to their natural state. Because the particles are analyzed separately, the native structure is not distorted by non-natural circumstances. Hence, computational sorting of diverse conformations of proteins or protein complexes in a given preparation enable the visualization of various intermediate states and can provide kinetic information about a specific interaction between individual molecules. As XRD and NMR techniques report an average result based on the summed signal of a large population of individual molecules, such events might be overlooked. The main prerequisite for Cryo-EM is a homogeneous distribution of particles in a thin layer of ice. The potential initial model obtained from negative stain TEM (see above) can be a starting point for the structure refinement.

Recently, Cryo-TEM has moved to near atomic resolution through new developments in detectors (direct electron detection cameras), novel imaging techniques (de-blurring via movie mode of direct electron detectors), contrast enhancement (phase plates), minimal contamination environment, and electron optical aberration correction techniques. NovAlix provides access to such technology through its Cryo-TEM sample analysis program. Our area of expertise includes the stabilization of the biological complexes using advanced screening methods (ProteoPlex), cryo sample preparation suitable for high resolution TEM, acquisition of large data sets, 2D classification of particles and 3D reconstruction of models. If component structures solved by X-Ray or NMR are available, we will fit them into the 3D EM model density map to display structural features that could not be revealed by either technique separately.

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Native MS
Native Mass Spectrometry
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Native mass spectrometry (native MS) is a robust and reliable tool for the analysis of non-covalent complexes. This label-free analytical technique also referred to as non-covalent, non-denaturing or supramolecular MS, utilizes electrospray to ionize and transfer non-covalent assemblies from solution to the gas phase. Accurate... Show more »

Native mass spectrometry (native MS) is a robust and reliable tool for the analysis of non-covalent complexes. This label-free analytical technique also referred to as non-covalent, non-denaturing or supramolecular MS, utilizes electrospray to ionize and transfer non-covalent assemblies from solution to the gas phase. Accurate measurement of the molecular mass of an intact complex provides valuable insights into biomolecule interaction, including stoichiometry, reversibility, site-specificity, affinity and polar contributions to the binding.

Native MS is widely applicable to a variety of biomolecular assemblies including multi-protein and nucleic acid systems, as well as to small molecules complexed to proteins or nucleic acids.

Combining medium throughput screening capability with the delivery of high-information-content, native MS complements other methods SPR, NMR, X-ray crystallography and ITC commonly used in drug discovery programs for hit finding and hit-to-lead optimization. Experimental conditions require optimization so there is no substitute for expertize and experience to routinely produce reliable data.

Reliable native MS data requires:

  1. Sample preparation, QC
  2. Mass spectrometer – operational parameters
  3. Automation

1) Sample preparation and QC

Sample preparation is the key to high quality mass spectra. Proteins stored in cocktails of non-volatile components, salts, buffers and surfactants are incompatible with electrospray ionization. Buffer exchange against a volatile ammonium-based buffer is a prerequisite for protein native MS.

Quality control of the desalted sample under denaturing and non-denaturing conditions provides information of sample homogeneity and molecular mass, conformation (folded versus unfolded), and oligomerization state. Analysis under native conditions may also uncover the presence of natural or fortuitous ligands that happen to have co-purified with the protein sample.

2) The mass spectrometer

Electrospray ionization (ESI) is the best-suited source of non-covalent complex ions from aqueous solutions.

For native MS applications, ESI sources are advantageously coupled to time-of-flight (TOF) mass analyzers which combine speed, resolution, sensitivity and broad mass range.

The analysis of non-covalent complexes using ESI-TOF instruments requires fine tuning of acceleration voltage and gas pressure to provide optimal ion desolvation and transmission while preserving the integrity of non-covalent complexes [Vivat Hannah et al., Future Medicinal Chemistry 2010, 2, 35].

3) Automation

Hyphenation of ESI-TOF mass spectrometers with a Triversa Nanomate® automated chip-based nanoESI robotic interface achieves remarkable throughput allowing daily screening of 384 compounds or cocktails, consuming only 50-200 pmol of protein per well. Subsequent raw data processing has been significantly accelerated by in-house developed programs allowing automated hit compound assignment.

Features and benefits

Requirements

  • Protein, nucleic acid or compound labeling/immobilization: NO
  • Purified, homogeneous and soluble targets: YES

Strengths

  • Sensitivity: detection of nanomolar complex concentrations.
  • Selectivity: species are clearly identified through accurate mass measurement (no data modeling or fitting is required); multiplexing – the ability to provide evidence in a single mass spectrum of several assemblies present together in solution.
  • Speed: analyses take only few minutes of data acquisition per sample affording medium throughput capability.

Limitations

  • Hydrophobic complex: partial or complete in source dissociation (false negatives)
  • Screening for low affinity binders (Kd > 100 µM): false positives due to non-specific aggregation of compounds during the ESI process.
  • Buffer compatibility is limited to volatile ammonium-based buffers
  • Not easily applicable to whole membrane proteins such as GPCRs
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X-ray crystallography
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Access to accurate high-resolution molecular structures is essential for the understanding of biological processes and to support drug discovery programs.
X-ray crystallography is the gold standard for the structural determination of protein complexes with other proteins, nucleic acids and small organic ligands.
X-ray... Show more »

Access to accurate high-resolution molecular structures is essential for the understanding of biological processes and to support drug discovery programs.
X-ray crystallography is the gold standard for the structural determination of protein complexes with other proteins, nucleic acids and small organic ligands.
X-ray crystallography can reliably provide the answer to many structure related questions, from global folds to atomic details of bonding, without any size limitation of the complex. Precise interactions between protein and ligand can be visualized with atomic resolution giving profound insight into the mechanism of molecular recognition, an approach that has revolutionized the rational design of new drugs.

NovAliX scientists are internationally recognized and valued by clients both for their state of the art problem solving skills as well as the efficient delivery of routine services.

Structures are determined for de novo targets and protein-ligand complexes. These are available as a stand-alone service or as part of a drug discovery package.

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NMR
Nuclear magnetic resonance spectroscopy
Price on request

NovAliX supports the in-house needs of the chemical synthesis teams with a dedicated Bruker 400 MHz NMR.

Our NMRTEC subsidiary, co-located with the NovAliX laboratories, provides expert NMR services and software products to industrial and academic clients across sectors spanning physical or material sciences and life sciences.... Show more »

NovAliX supports the in-house needs of the chemical synthesis teams with a dedicated Bruker 400 MHz NMR.

Our NMRTEC subsidiary, co-located with the NovAliX laboratories, provides expert NMR services and software products to industrial and academic clients across sectors spanning physical or material sciences and life sciences. NMRTEC operates within an ISO9001 (2008) quality system accredited by SQS (Switzerland). Some highlights are QC of chemical libraries; fragment screening for fragment based lead generation; quantification in simple or complex matrices including ppm level trace analysis; extensive characterization of hyaluronic acid; polymers, polymorphism, and stability studies.

Solution phase

Includes all standard nuclei, multi-dimensional experiments, qNMR and cryoprobes at 600 & 700 MHz.

Solid-state and HR-MAS NMR

Applied to solids, or for High-Resolution Magic Angle Spinning (HR-MAS), to gels and more generally to solutions where dipole-dipole interactions are insufficiently averaged owing to restricted internal molecular motion. Spinning at the magic angle aims to mimics the orientation averaging in solution that is critical for obtaining sharp signals.

NMR Instruments

Bruker 400, 500, 600 and 700 MHz

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Surface Plasmon Resonance (SPR)
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Surface Plasmon Resonance (SPR) is a versatile technique for the label-free reporter free studies of bio-molecular interactions. Artifacts and interferences commonly observed in fluorescence-based assays are avoided.

NovAliX’ SPR instruments are highly sensitive. Both commercial and proprietary systems are available with... Show more »

Surface Plasmon Resonance (SPR) is a versatile technique for the label-free reporter free studies of bio-molecular interactions. Artifacts and interferences commonly observed in fluorescence-based assays are avoided.

NovAliX’ SPR instruments are highly sensitive. Both commercial and proprietary systems are available with complementary advantages in throughput, format, workflow and information delivered. The high level features of these formats are as follows:

Commercial channel-based Biacore™ systems

  • Medium throughput ligand screening
  • Hit confirmation
  • Characterization of compound/protein binding
  • Detailed analysis protein/ligand Interactions

Proprietary NovAliX chemical microarray SPR

  • Higher throughput primary screening
  • Fragment based drug discovery
  • Affinity ligands for bio-separation (downstream processing)
  • A technology platform for molecular recognition

In each format, one of the potential binding partners is immobilized on a sensor surface whist the other is in solution. Binding of the soluble analyte to the immobilized partner induces a change in refractive index close to the surface that can be read by SPR. The sensor surfaces are gold coated which permits functionalization in conventional systems this enables the coupling of the protein of interest to the sensor while in the microarray format thousands of small molecules are immobilized on a single chip.

SPR assays support many applications in research, development and manufacturing. NovAliX has considerable experience in the use of SPR for drug discovery and bio-separation. Measurements include binding affinity, binding kinetics and selectivity. Modern instruments are even sensitive enough to detect weak binding of low molecular weight analytes, a prerequisite for fragment based drug discovery.

NovAliX uses commercial channel-based GE BiacoreTM instruments but the array-based systems are exclusively designed & produced for in-house use. This combination creates more opportunities to address challenging issues.

Biacore™ Analysis Service

NovAliX utilizes state of the art SPR systems like the Biacore™ 3000 and Biacore™ T200. We offer a comprehensive set of measurements including specificity, kinetics and affinity analysis (kon, koff, Kd). These characterizations of the interactions of biomolecules; antibodies, therapeutic proteins, enzymes and small molecules serve pharmaceutical industrial and academic partners.

Services include experimental design, setup, data acquisition and expert interpretation. All commonly used target immobilization techniques are available. Projects include the analysis of single target/analyte interactions (see also Compound characterization) and screening against NovAliX’ collection of fragments or libraries of soluble compound provided by our partners and clients.

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ESI-MS
Electrospray Ionization Mass Spectrometry
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