Dlgxbjoitf2dfnocgqa8 nova

NovaBioAssays LLC

9 Orders Completed
Woburn, Massachusetts, US

About NovaBioAssays LLC

Founded in 2012, NovaBioAssays LLC is a Greater Boston-based contract research organization (CRO) specialized in providing innovative analytical and bioanalytical solutions to facilitate drug discovery and development. NovaBioAssays LLC occupies the two-story building with 12,000 SF space, and 10,000 SF for future expansion. We offer a full suite of analytical and bioanalytical services for quantitative and qualitative analysis of small molecules, oligonucleotides, peptides, and proteins.

Our Services (21)


ic

Small Molecule Bioanalysis

Price on request
  • Small Molecule Quantification
  • Drug & Metabolites Quantification
  • Small Molecule Biomarker Assays
  • Drug Metabolite Identification and Profiling
  • ADME


ic

Protein Quantification

Price on request

Historically, immunoassays and other ligand-binding assays (LBA) have been the golden standard for quantifying proteins in biological fluids to support preclinical and clinical studies due to their high sensitivity and throughput. However, the resource and time consuming process for reagent production and selection routinely results in lengthy assay development period (>6 months). In addition, the selectivity of LBA is prone to be compromised by presence of endogenous interfering molecules in different matrices, especially in tissue samples. With recent development on mass spectrometry instrumentation and methodology, approaches for protein quantitation based on liquid chromatography mass spectrometry (LC-MS) have matured lately. LC-MS/MS assays have been increasingly used as the alternative for the absolute quantification of the endogenous biomarker proteins, and the therapeutic recombinant proteins or mAbs. LC-MS/MS assays are especially useful for situations when quantifying therapeutic proteins in human serum in the presence of pre-existing anti-drug antibodies; when required to quantify the therapeutic protein and its metabolites simultaneously; or when the reagents for LBA are not available. At NovaBioAssays, we provide a variety of LC-MS/MS-based platforms as well as traditional LBA methods to meet our clients’ bioanalytical needs.

  • Direct UPLC-MRM or UPLC-PRM/SIM
  • 2D UPLC UPLC-MRM or UPLC-HRMS
  • Immunoaffinity UPLC-MRM or UPLC-HRMS
  • Dual immunoaffinity nano UHPLC-MRM or nano UPLC-HRMS
  • Ligand Binding Assays


ic

Protein Characterization

Price on request
  • Intact Molecular Weight Determination
  • Peptide Mapping
  • Post Translational Modification(PTM) Analysis
  • Disulfide Bridge Mapping
  • De Novo Sequencing
  • Glycosylation Analysis and Glycosylation Site Mapping/Occupancy Determination
  • Protein Identification


ic

Epitope Mapping

Price on request

Hydrogen Deuterium eXchange Mass Spectrometry (HDX-MS) is a powerful technique to study protein dynamics, protein folding, protein-protein interactions, protein small molecule interactions, as well as protein-RNA interactions. Novabioassays has built its reputation in providing HDX MS service for epitope mapping, biosimilarity comparison, studying small molecule-protein interactions, peptide-protein interactions, protein-protein interactions, and RNA-protein interactions for many small and large pharmaceutical companies globally in the past five years.

At Novabioassays, we have achieved almost 100% successful rate in performing epitope mapping for more than 200 antibodies targeting more than 40 different antigens (from ~10 kDa to ~200 kDa) to support our clients’ patent applications and/or IND filings.


ic

Metabolite Profiling

Price on request

NovaBioAssays uses advanced liquid chromatographic assays and ultrasensitive ELISA to quantify oligonucleotide therapeutics in biological matrices. While some ELISA assays are more sensitive, the chromatographic based methods are more specific and able to quantify multiple analytes in one assay. The chromatographic based assays can also conduct metabolite identification and/or quantification simultaneously.

  • Quantitative bioanalysis using UPLC-MS/MS assays on triple quadrupole mass spectrometer (Sciex API5000 or Thermo Quantiva) or UPLC-HRAM (Thermo Q Exactive-Plus)
  • Quantitative bioanalysis using ultrasensitive fluorescence ELISA
  • Quantitation and metabolite identification using LC-HRAM on Q Exactive or Q-Tof
  • Quantitation and metabolite profiling using hybridization based UPLC-fluorescence assays
  • Quantitation and metabolite profiling using UPLC-UV(PDA)-MS
  • Structure characterization and purity assessment of CRISPR sgRNA using LC-HRAM on Q Exactive or Q-Tof


ic

Biomarker Analysis

Price on request
  • Small molecule biomarker
    • Neurotransmitters (dopamine, nephrine, serotonin etc.)
    • Lipids
    • Polyamine
    • Amino acids
    • Hormones
  • Protein biomarkers
  • Oligomannose


ic

Antibody-Drug Conjugate (ADC) Analysis

Price on request

NovaBioAssays provides the LC-MS or LC-MS/MS based analysis for Antibody-Drug Conjugates (ADCs) and other conjugates, such as bispecific antibodies, Fc fusion proteins, antibody fragments (Fab, Fc, and Nanobodies), etc.

Services for ADCs and other conjugated products include:

  • Drug-to-Antibody Ratio (DAR) Determination
  • Site Occupancy
  • Linkage site identification
  • Analysis of Free Drug (Payload) and Naked Carrier Protein/Antibody
  • Quantification of Drug-Conjugate Components


ic

Biopharmaceutical Characterization

Price on request

NovaBioAssays emphasizes the Reference Standard and Biosimilarity Characterization via LC-MS and LC-MS/MS technology, including:

  • nIntact Mass measurement
  • Peptide Mapping
  • Glycan Profiling
  • Post-translational Modification Analysis
  • Biosimilarity with HDX MS


ic

ADME/DMPK Studies

Drug Metabolism and Pharmacokinetics
Price on request
  • Whole blood or plasma stability test
  • Microsomal and hepatocyte stability evaluation
  • Metabolite identification/profiling
  • Enzymatic reaction phenotyping
  • Protein binding evaluation using ultracentrifugation or RED (rapid equilibrium dialysis)


ic

Bioanalysis

Price on request
  • Independent Quality Assurance
  • Validated and calibrated equipment
  • Method development and validation
  • Validated Watson LIMS 7.5 software
  • Validated AB Sciex Analyst 1.6.2 software
  • Validated Rees Scientific Environment Temperature and Moisture Monitoring


ic

Drug Discovery & Development

Price on request

Drug Discovery & Development Services


ic

Pharmacology & Toxicology

Price on request

Pharmacology & Toxicology Services


ic

Pharmacology

Price on request

Pharmacology Services


ic

Biology

Price on request

Biology Services


ic

Biochemistry & Molecular Biology

Price on request

Biochemistry & Molecular Biology Services


ic

Protein Services

Price on request

Protein Services


ic

Antibody Services

Price on request

Antibody Services


ic

Biomarkers

Price on request

Biomarkers Services


ic

Omics

Price on request

Omics Services


ic

Metabolomics

Price on request

Metabolomics Services


ic

Protein Purification

Price on request

Protein Purification and Quantification Services


Not finding what you're looking for?

Get info on this provider's capabilities without requesting a quote.
CJ

Chengjie Ji

President

NovaBioAssays LLC has not received any reviews.

NovaBioAssays LLC has not received any endorsements.