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NextGen Sequencing Core (MGH)

Boston, Massachusetts, US

The NextGen Core is a collaboration between the Department of Molecular Biology, the Center for Human Genetics Research, the Center for Computational Biology, and the Executive Committee on Research (ECOR).

Currently, the Core operates using a single Ilumina HiSeq instrument, accompanied by Illumina's cBot for cluster generation. This upgrade from our Genome Analyzer II doubled our capacity and greatly increased the data amount, quality, and stability over extra-long reads.

The Core is located in the state-of-the-art Richard Simches Research Center on Cambridge St. as part of the MGH main campus in Boston. The many multi-investigator groups in the building - including those that study human genetics, stem cells, genomics, and more - make it the perfect location for the Core to service the researchers in those groups. The majority of customers come from MGH, but we also service customers at other academic medical centers and industry.

NextGen Sequencing Core (MGH) has not listed any services.

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DNA Sequencing Data Analysis
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Illumina Sequencing
Starting at $1,860.00 per sample

Price per lane of sequencing includes library quality control, cluster generation, sequencing, standard Illumina barcode reads, and online delivery of sequence and quality data.

Single 50: $1290 (internal); $1860 (external academic); $2060 (industry)
Paired 50: $1820 (internal); $2630 (external academic); $2900... Show more »

Price per lane of sequencing includes library quality control, cluster generation, sequencing, standard Illumina barcode reads, and online delivery of sequence and quality data.

Single 50: $1290 (internal); $1860 (external academic); $2060 (industry)
Paired 50: $1820 (internal); $2630 (external academic); $2900 (industry)
Single 100: $1580 (internal); $2280 (external academic); $2520 (industry)
Paired 100: $2310 (internal); $3330 (external academic); $3680 (industry)
Paired 75: $2110 (internal); $3040 (external academic); $3360 (industry)

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Illumina NGS Library Preparation
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Many researchers prepare their own libraries using unique or experimental protocols. Alternatively, the core facility can prepare the libraries for you. The core can work with you to identify or devise a library construction method that fits your needs. The lab is prepared to create genomic and ChIP-seq libraries, and is gearing... Show more »

Many researchers prepare their own libraries using unique or experimental protocols. Alternatively, the core facility can prepare the libraries for you. The core can work with you to identify or devise a library construction method that fits your needs. The lab is prepared to create genomic and ChIP-seq libraries, and is gearing up to offer mRNA, sRNA, strand-specific mRNA, as well as some target enrichment library construction strategies, such as the Agilent SureSelect system (bait sequences not supplied).

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Agilent SureSelect
NGS data analysis
Next generation sequencing data analysis
Price on request

Illumina's HiSeq software automatically makes base calls and generates FASTQ sequence and quality files, which are delivered to you. As a separate service, the core's experienced bioinformatics team can perform custom analysis and alignments.

Illumina's HiSeq software automatically makes base calls and generates FASTQ sequence and quality files, which are delivered to you. As a separate service, the core's experienced bioinformatics team can perform custom analysis and alignments.

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DNA Sequencing
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Biology
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Sequencing Data Analysis
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Nucleic Acid Analysis
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Nucleic Acid Data Analysis
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Next Generation Sequencing (NGS)
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DNA
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DNA Library Construction Services
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Biochemistry & Molecular Biology
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Nucleic Acid Services
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