NextGen Sequencing Core (MGH)
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Boston, Massachusetts, US
The NextGen Core is a collaboration between the Department of Molecular Biology, the Center for Human Genetics Research, the Center for Computational Biology, and the Executive Committee on Research (ECOR).
Currently, the Core operates using a single Ilumina HiSeq instrument, accompanied by Illumina's cBot for cluster generation. This upgrade from our Genome Analyzer II doubled our capacity and greatly increased the data amount, quality, and stability over extra-long reads.
The Core is located in the state-of-the-art Richard Simches Research Center on Cambridge St. as part of the MGH main campus in Boston. The many multi-investigator groups in the building - including those that study human genetics, stem cells, genomics, and more - make it the perfect location for the Core to service the researchers in those groups. The majority of customers come from MGH, but we also service customers at other academic medical centers and industry.
Price per lane of sequencing includes library quality control, cluster generation, sequencing, standard Illumina barcode reads, and online delivery of sequence and quality data.
Single 50: $1290 (internal); $1860 (external academic); $2060 (industry)
Paired 50: $1820 (internal); $2630 (external academic); $2900 (industry)
Single 100: $1580 (internal); $2280 (external academic); $2520 (industry)
Paired 100: $2310 (internal); $3330 (external academic); $3680 (industry)
Paired 75: $2110 (internal); $3040 (external academic); $3360 (industry)
Many researchers prepare their own libraries using unique or experimental protocols. Alternatively, the core facility can prepare the libraries for you. The core can work with you to identify or devise a library construction method that fits your needs. The lab is prepared to create genomic and ChIP-seq libraries, and is gearing up to offer mRNA, sRNA, strand-specific mRNA, as well as some target enrichment library construction strategies, such as the Agilent SureSelect system (bait sequences not supplied).
Illumina's HiSeq software automatically makes base calls and generates FASTQ sequence and quality files, which are delivered to you. As a separate service, the core's experienced bioinformatics team can perform custom analysis and alignments.
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