Nexomics proudly offers gene-to-structure services to the biopharmaceutical and biological research community. With a strong focus on discovery and analytical R&D services, our core capabilities include target selection and construct design using bioinformatics tools, cloning of genes into protein production vectors, recombinant protein expression, laboratory-scale fermentation, protein purification, crystallization screening, NMR structural studies, X-ray crystallographic structural studies, and functional assay development.
Gold L, Ayers D, Bertino J, Bock C, Bock A, Brody EN, Carter J, Dalby AB, Eaton BE, Fitzwater T, Flather D, Forbes A, Foreman T, Fowler C, Gawande B, Goss M, Gunn M, Gupta S, Halladay D, Heil J, Heilig J, Hicke B, Husar G, Janjic N, Jarvis T, Jennings S, Katilius E, Keeney TR, Kim N, Koch TH, Kraemer S, Kroiss L, Le N, Levine D, Lindsey W, Lollo B, Mayfield W, Mehan M, Mehler R, Nelson SK, Nelson M, Nieuwlandt D, Nikrad M, Ochsner U, Ostroff RM, Otis M, Parker T, Pietrasiewicz S, Resnicow DI, Rohloff J, Sanders G, Sattin S, Schneider D, Singer B, Stanton M, Sterkel A, Stewart A, Stratford S, Vaught JD, Vrkljan M, Walker JJ, Watrobka M, Waugh S, Weiss A, Wilcox SK, Wolfson A, Wolk SK, Zhang C, Zichi D. Aptamer-based multiplexed proteomic technology for biomarker discovery. PLoS One. 2010 Dec 7;5(12):e15004. doi: 10.1371/journal.pone.0015004.
Nexomics offers protein expression services at various scales (small, midi, and large scales, up to 30 L Bioreactor scale).
We support a wide range of expression systems and media types (LB / TB with Animal-Free Soytone, MJ9).
We have unique expertise in preparing stable isotope-enriched media (15N, 15N13C, 15N13C2D-ILV, 19F) for protein specific isotope labeling.
Nexomics uses ÄKTA Protein Purification Systems to obtain the highest purity protein samples.
Large scale protein purification (up to grams range) using affinity or ion exchange chromatography (up to 500 ml column volume).
Molecular weight is confirmed by MALDI-TOF Mass Spectrometry.
Nexomics has deep experience with NMR structural biology techniques. We offer a full range of services from data collection, data processing, data analysis and structure determination.
Backbone Resonance Assignments
Sidechain Resonance Assignments
Protein-Protein and Protein-Ligand Interactions
Protein Structure Determination
Small Molecule Screening
Structure mapping by NMR
Quality control and Quantitative analysis
Nexomics has extensive experience solving protein crystal structures, including protein-protein, and protein-nucleic acid complexes, using X-ray crystallography.
Diffraction Data Collection
Protein Structure Determination
Homology models are useful for pathway modeling, predictive protein-protein / protein-ligand interactive simulations and in general as a guide in the protein structure determination process. Nexomics Biosciences offers homology modeling using our proprietary modeling and structure validation software.
Nexomics utilizes a number of experimental biophysical techniques to ensure quality control as well as for further understanding of the biological system under study.
Designing the correct primer sequence can have a large impact on the success of a PCR reaction.
Through our automated proprietary primer design software we can design and deliver the optimal set of primers to ensure success.
Gene Synthesis is a powerful new technology for delivering difficult-to-isolate genes.
Gene synthesis is particularly useful when applying domain parsing techniques, mutagenesis techniques, or when working with difficult-to-isolate eukaryotic cDNA pools.
MALDI-TOF mass spectroscopy is used as a quality control check for all protein production projects. The technology can also be used to measure regions of disorder within a protein.
Light scattering data are useful for confirming oligomerization states of protein (e.g., monomer, dimer, etc.). And for determining shape-independent molecular weights.
Lights scattering studies are also valuable for construct optimization to enhance crystallization propensities of proteins.
BLI optical analytical technique to evaluate and characterize bimolecular interactions.
It can be used in assay development and to determine protein interaction affinities.
Protein specific stable isotope labeling Services
Nexomics has extensive experience incorporating stable isotopes (e.g. 15N, 13C, 2H, 19F) into native protein to facilitate NMR and mass spectrometry studies.
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