NeuroSolutions Ltd

In vitro slice electrophysiology

Almost all brain regions, spinal cord, naïve and model animals, single/dual recordings

Combined with electrophysiological experiments, Cerebrasol scientists can offer behavioural test to quantify effects of lead compounds on behavioual endpoints

• Field excitatory post-synaptic potentials (fEPSPs)
• Population spikes (PS)
• Long-Term Potentiation (LTP)
• Long-Term Depression (LTD)
• Schaffer-collateral CA1 pathway
• Medial/lateral perforant dentate gyrus pathway
• Dentate gyrus CA3 ‘Mossy Fibre’ pathway
• Transgenic capability

In vivo electrophysiology

Almost all brain regions, spinal cord, nerve fibres single fibre level, naïve and model animals

Neurosolutions has a wealth of experience in providing first class preclinical translational research into the study, treatment and prevention of mental disorders. Our track record can be demonstrated in our manuscript publications and our key strength is in developing precisely tailored research packages to target out client’s needs. Whether it is in vitro or in vivo electrophysiological approaches, whole animal behavior or molecular biology techniques, our research teams will provide expert consultancy in putting together the study proposal you require, and our investigators will then rapidly deliver data of the highest quality to drive forward your research programs.

Disease Areas

• Schizophrenia
• Depression
• Anxiety
• Psychosis

Behavioural Tests

• Barnes maze
• Conflict anxiety model (high level illumination & odour)

Electrophysiological Assays

• In vivo: single unit recordings from hippocampus/ striatum/ substantia nigra/ dorsal raphe/ prefrontal cortex / brainstem Local field potential/population spike recording from hippocampus; CA1/DG/CA3, LTP, MEA
• In vitro: whole-cell patch-clamp recordings from brain slices (current/voltage/blind/visualised)
• Local field potentials – spontaneous and evoked extracellular recordings from brain slices, including LTP/LTD and multi-electrode recording (MEA) Ion channel pharmacology – state-dependency, kinetics, and site of action using cultured and primary dissociated neurones, cell lines

Neurosolutions has established expertise in delivering first class preclinical translational research into pain, particularly around the development of new treatments into neuropathic and inflammatory pain.

Our track record can be demonstrated in our manuscript publications and our key strength is in developing precisely tailored research packages to target out client’s needs. Whether through using in vitro or in vivo electrophysiological approaches to establish mechanism, using whole animal behavior in one of our many pain models to screen compounds, or using molecular biology techniques to better understand function, our research teams will provide expert consultancy in putting together the study proposal you require, and our investigators will then rapidly deliver data of the highest quality to drive forward your research programs.

Diesease Areas

• Chronic constriction injury
• Spinal nerve ligation
• Diabetes-induced
• Chemotherapy-induced
• Fracture pain model
• Carrageenan
• Complete Freund’s adjuvant
• Formalin
• MIA
• UVB
• Surgical pain

Behavioural Tests

• Von Frey hair
• Hargreave’s
• Randall-Selitto
• Weight-bearing
• Paw licking, shaking, lifting
• Plethysmography
• RotaRod test

Electrophysiological Assays

• In vivo: Single fibre recording, spontaneous and evoked, single neurone recording from the spinal cord and brain, all naïve and modelled animals
• In vitro: Compound action potential recording from isolated nerves, naïve and modelled, slice patch-clamp recordings, dissociated DRG patch-clamp recordings from naïve and modelled animals

Neurosolutions has extensive experience in delivering first class preclinical translational research into the study, treatment and prevention of neurodegenerative disorders, particularly around amyloid based animal models of Alzheimer’s disease.

Our track record is demonstrated in our manuscript publications and our key strength is in developing precisely tailored research packages to target out client’s needs. Whether it is in vitro or in vivo electrophysiological approaches, whole animal behavior or molecular biology techniques, our research teams will provide expert consultancy in putting together the study proposal you require, and our investigators will then rapidly deliver data of the highest quality to drive forward your research programs.

Disease Areas

• Alzheimer’s Disease
• Parkinson’s Disease
• Multiple Sclerosis
• Huntington’s Disease

Behavioural Tests

• Open field: e.g. object recognition test (ORT), Y-maze, Barnes maze
• Operant chambers: e.g. Alternating Lever Cyclic Ratio (ALCR), delayed match-to-position (DMTP/DNMTP)
• Beta-amyloid induced deficits (ADDL/APP/oligomers/aggregated) α-synuclein-induced deficits
• Commercially available transgenic rodent models

Electrophysiological Assays

• In vivo: single cells – extra and intracellular recording; iontophoretic, central and parenteral drug administration
• Local field potentials – spontaneous and evoked, including LTP/LTD
• Peripheral nerves – compound and single fibre
• Telemetric and multi-electrode recording
• In vitro: whole-cell patch-clamp recordings from brain slices (current/voltage/blind/visualised)
• Local field potentials – spontaneous and evoked extracellular recordings from brain slices, including LTP/LTD and multi-electrode recording (MEA)
• Ion channel pharmacology – state-dependency, kinetics, and site of action using cultured and primary dissociated neurones, cell lines

Animal Model in vivo Analyses Services

Animal Cognition & Behavior Tests Services

Animal Learning, Memory, and Behavior Tests Services