1365775796520 f5e83205b3df6f6d3023230358dd1003

Neuro-Zone srl

Milan, IT

Neuro-Zone srl supports drug discovery and development by providing physiologically relevant cell systems and multiparametric cell based assays for preclinical projects. We act as an outsourced high quality scientific lab which supports biotech/pharmas in experimental layout, physically running experiments in-house and carrying out data analysis and interpretation.

We develop pathology specific platforms for drug discovery, based on our MicroTISSUE technology.
MicroTISSUE dissects cell-cell contribution in complex inflammatory scenarios. These platforms are utilized in diverse lead optimization screening programs (cancer, neurodegenerative diseases, autoimmune diseases, cardiovascular, skin and ophthalmic diseases).
MicroTISSUE generates rapid and informative data on efficacy, toxicity, metabolism and compound-receptor binding effects as well as understanding of molecular mechanisms associated with response to... Show more »

Neuro-Zone srl supports drug discovery and development by providing physiologically relevant cell systems and multiparametric cell based assays for preclinical projects. We act as an outsourced high quality scientific lab which supports biotech/pharmas in experimental layout, physically running experiments in-house and carrying out data analysis and interpretation.

We develop pathology specific platforms for drug discovery, based on our MicroTISSUE technology.
MicroTISSUE dissects cell-cell contribution in complex inflammatory scenarios. These platforms are utilized in diverse lead optimization screening programs (cancer, neurodegenerative diseases, autoimmune diseases, cardiovascular, skin and ophthalmic diseases).
MicroTISSUE generates rapid and informative data on efficacy, toxicity, metabolism and compound-receptor binding effects as well as understanding of molecular mechanisms associated with response to candidate drugs.

Moreover, it enables effective selection and ranking of the most promising candidates per indication
Drug development is time consuming, costly and inefficient. By increasing the quality of cell-based assays NeuroZone enables more efficient and informative drug screening programs.

www.neuro-zone.com

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Mitochondrial Complex IV Assay
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MITOS PLATFORM - Quantitative evaluation of Cytochrome c oxidase (COX) activity (complex IV).

Assay
Quantitative evaluation of Cytochrome c oxidase (COX) activity (complex IV).

Aim
Cytochrome c oxidase (COX) activity is used to determine the activity of the respiratory Complex IV and can represent an indicator of the... Show more »

MITOS PLATFORM - Quantitative evaluation of Cytochrome c oxidase (COX) activity (complex IV).

Assay
Quantitative evaluation of Cytochrome c oxidase (COX) activity (complex IV).

Aim
Cytochrome c oxidase (COX) activity is used to determine the activity of the respiratory Complex IV and can represent an indicator of the oxidative capacity of the cells.

Description
COX is the terminal enzyme complex of the electron transport system and catalyzes the transfer of four electrons from cytochrome c to molecular oxygen, which is reduced to two molecules of water.
This transmembrane complex is located at the inner mitochondrial membrane and, in mammals, is composed by 13 different subunits.
The catalytic core of this complex is composed by three subunits encoded by mitochondrial-DNA, while the remaining ten subunits encoded by nuclear-DNA are located around the core and have regulatory function.
COX activity is regulated by a wide spectrum of physiological and pathological factors and many dysfunctions are invariably associated with increased mitochondrial reactive oxygen species production and cellular toxicity.
Under normal physiological conditions, COX acts as the rate limiting step of respiratory chain and its activity is an indicator of the oxidative capacity of the cells.
Cytochrome c oxidase activity is measured by following the decrease in absorbance due to the oxidation of ferrocytochrome c (ε = 18.7 mM−1 · cm−1).
KCN is added to inhibit cytochrome c oxidase activity, which is considered as the cyanide-sensitive rate of cytochrome c oxidation.
The reduction in absorbance at 550 nm is measured using a spectrophotometer

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Oxidoreductase Assays
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MITOS PLATFORM - Cyt c oxido-reductase activity.

Assay
Quantitative evaluation of mGPDH: Cyt c oxido-reductase activity.

Aim
mGPDH: Cyt c oxido-reductase activity is used to determine the activity of mytochondrial Glycerol-3-phosphate dehydrogenase.

Description
mGPDH is an enzyme embedded on the outer surface of the... Show more »

MITOS PLATFORM - Cyt c oxido-reductase activity.

Assay
Quantitative evaluation of mGPDH: Cyt c oxido-reductase activity.

Aim
mGPDH: Cyt c oxido-reductase activity is used to determine the activity of mytochondrial Glycerol-3-phosphate dehydrogenase.

Description
mGPDH is an enzyme embedded on the outer surface of the inner mitochondrial membrane that catalyzes the irreversible oxidation of glycerol-3-phosphate to dihydroxyacetone phosphate and concomitantly transfers two electrons from FAD to the electron transport chain.
mGPDH is a very important enzyme of intermediary metabolism and as a component of glycerophosphate shuttle it functions at the crossroads of glycolysis, oxidative phosphorylation and fatty acid metabolism.
Alteration of mGPDH function appears to be associated with several pathological states.

mGPDH: Cyt c oxido-reductase activity was measured as the increase in absorbance at 550 nm due to the ferricytochrome c reduction in the presence of cytochrome c, Glycerol-3-phosphate and KCN.
Antimycin A is added to inhibit complex III activity and the mGPDH activity is considered as the antimycin-sensitive rate of cytochrome c reduction (ε = 18.7 mM−1 · cm−1).
The increase in absorbance at 550 nm is measured using a spectrophotometer

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Mitochondrial Complex I Assay
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MITOS PLATFORM - Mitochondrial Complex I + III Assay

Assay
Quantitative evaluation of complex I+III (NADH: Cyt c oxido-reductase activity).

Aim
Complex I+III activity is used to evaluate the activity of the first complex of the mitochondrial electron transfer chain.

Description
Complex I (NADH dehydrogenase) is an... Show more »

MITOS PLATFORM - Mitochondrial Complex I + III Assay

Assay
Quantitative evaluation of complex I+III (NADH: Cyt c oxido-reductase activity).

Aim
Complex I+III activity is used to evaluate the activity of the first complex of the mitochondrial electron transfer chain.

Description
Complex I (NADH dehydrogenase) is an enzyme of the respiratory chains present in several organisms from bacteria to humans.
The enzyme oxidizes NADH transferring electrons to Ubiquinone (Coenzyme Q, CoQ), a lipid soluble electron carrier embedded in the lipid bilayer of the inner mitochondrial membrane.
Mitochondrial complex I activity (NADH:ubiquinone oxidoreductase) has been considered difficult to assay, largely because of the inaccessibility of the complex in the inner mitochondrial membrane and the insolubility of the ubiquinone.
For this reason it is preferred to evaluate the activity of the I + III complexes (NADH: Cytc oxido-reductase activity).
Complex I dysfunction alone, account for approximately half all defects in oxidative phosphorylation and variations of its activity are described in various pathologies.
NADH: Cyt c oxido-reductase activity is measured as the increase in absorbance at 550 nm due to the ferricytochrome c reduction in the presence of cytochrome c, NADH and KCN.
Rotenone is added to inhibit complex I activity, which is considered as the rotenone-sensitive rate of cytochrome c reduction (ε = 18.7 mM−1 · cm−1).
The increase in absorbance at 550 nm is measured using a spectrophotometer.

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Oxidative Phosphorylation Assay
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MITOS PLATFORM - Oxidative Phosphorylation Assay

Assay
Analyses of the oxidative phosphorylation system.

Aim
Analyses of the oxidative phosphorylation system is used for the characterization of mitochondrial respiratory capacity.

Description
The oxidative phosphorylation system consists of five multimeric complexes... Show more »

MITOS PLATFORM - Oxidative Phosphorylation Assay

Assay
Analyses of the oxidative phosphorylation system.

Aim
Analyses of the oxidative phosphorylation system is used for the characterization of mitochondrial respiratory capacity.

Description
The oxidative phosphorylation system consists of five multimeric complexes embedded in the mitochondrial inner membrane. In addition to the complexes of the electron transport chain, there are other enzymes (dehydrogenases) able to supply electrons directly to ubiquinone. These electrons will then be transferred to complex III and subsequently to complex IV. All these enzymatic complexes work in concert to drive the aerobic synthesis of ATP. Mitochondrial and nuclear DNA mutations affecting the accumulation and function of these enzymes are the most common cause of mitochondrial diseases and have also been associated with neurodegeneration and aging.
The tests that can be done to characterize the mitochondrial respiratory chain enzymes are:
• Complex I+III
• Glycerol-3-phosphate dehydrogenase + III
• Proline dehydrogenase + III
• Complex II + III
• Complex III
• Complex IV
• Complex V
These assays can be performed in mitochondria-enriched fractions prepared from tissues or cultured cells, in tissue homogenates, or in whole cells.

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Mitochondrial Toxicity Studies
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MITOS PLATFORM - Citrate Synthase activity

Assay
Quantitative evaluation of Citrate synthase (CS) activity

Aim
CS activity is used to evaluate the mitochondrial content.

Description
Citrate synthase is the enzyme that catalyzes the first reaction of the Krebs cycle and is localized in the mitochondrial... Show more »

MITOS PLATFORM - Citrate Synthase activity

Assay
Quantitative evaluation of Citrate synthase (CS) activity

Aim
CS activity is used to evaluate the mitochondrial content.

Description
Citrate synthase is the enzyme that catalyzes the first reaction of the Krebs cycle and is localized in the mitochondrial matrix.
Quantitative evaluation of its activity is used to estimate the mitochondrial content which is an important indicator of oxidative capacity.
CS activity is also used to normalize other bioenergetics parameters.
Low CS activity represents a low oxidative capacity.
CS catalyzes the condensation reaction of the two-carbon acetate residue from acetyl coenzyme A and a molecule of four-carbon oxaloacetate to form the six-carbon citrate.
The hydrolysis of the thioester of acetyl CoA results in the formation of CoA with a thiol group (CoA-SH).
The CoASH reacts with the DTNB to form 5-thio-2-nitrobenzoic acid (TNB), a yellow product that is monitored spectrophotometrically at 412 nm using a spectrophotometer

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Oxidative Stress Assays
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MITOS PLATFORM - Acotinase activity

Assay
Quantitative evaluation of Aconitase activity.

Aim
Aconitase activity is used as a biomarker for oxidative damage.

Description
Aconitase is an iron-sulfur containing enzyme that catalyzes the isomerization of citrate to isocitrate via cis-aconitate in the Krebs... Show more »

MITOS PLATFORM - Acotinase activity

Assay
Quantitative evaluation of Aconitase activity.

Aim
Aconitase activity is used as a biomarker for oxidative damage.

Description
Aconitase is an iron-sulfur containing enzyme that catalyzes the isomerization of citrate to isocitrate via cis-aconitate in the Krebs cycle.
Aconitase activity is commonly used as a biomarker for oxidative stress and has been suggested to serve as an intramitochondrial sensor of redox status.
Aconitase enzymatic activity is assayed by measuring the conversion of cis-aconitate to isocitrate.
The assay monitors the disappearance of cis-aconitate as measured spectrophotometrically

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Mitochondrial Stress Tests
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MITOS PLATFORM - Total reduced Thiol test

Assay
Determination of total reduced thiol content.

Aim
Total Thiol assay is used to determine the antioxidant capacity of a cells.

Description
Thiols represent the largest part of the overall antioxidant pool in the cells and therefore play a major role in protecting against... Show more »

MITOS PLATFORM - Total reduced Thiol test

Assay
Determination of total reduced thiol content.

Aim
Total Thiol assay is used to determine the antioxidant capacity of a cells.

Description
Thiols represent the largest part of the overall antioxidant pool in the cells and therefore play a major role in protecting against damage from reactive oxygen species (ROS) and other oxidant compounds such as reactive nitrogen species (RNS).
Then the determination of total thiol reduced of cell extracts may be a useful parameter of assessment of oxidative stress.
In cells, thiol groups exist as free cysteine, glutathione and cysteine residues in proteins. Total reduced thiol content is assayed using the thiol reagent 5-5dithiobis2nitrobenzoic acid which reacts with the thiol groups forming a mixed disulfide and the and 2-nitro-5-thiobenzoic acid (TNB) which is quantified at 412 nm.

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Phagocytosis Assay
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Cells are challenged with appropriate stimuli, then fluorescein-labeled bioparticles are administered to cells, and modulation of phagocytic activity is quantitatively assayed.
An intraexperimental triplicate is carried out. Moreover, three independent experiments are performed.
Prices are comprehensive of scientific support for... Show more »

Cells are challenged with appropriate stimuli, then fluorescein-labeled bioparticles are administered to cells, and modulation of phagocytic activity is quantitatively assayed.
An intraexperimental triplicate is carried out. Moreover, three independent experiments are performed.
Prices are comprehensive of scientific support for the whole duration of the project:
• In depth analysis of scientific literature
• Sample handling and cell isolation
• Scientific operative consultancy throughout the project
• Sharing of detailed protocols used in the project
• Definition of key experimental parameters
• Data analysis and report
• Raw data delivery

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Neuropathic Pain Animal Models
Price on request

CHEMOTHERAPY-INDUCED NEUROPATHIC PAIN

Background

Chemotherapy-induced neuropathic pain is one of the most serious complications associated with anticancer
drugs. It leads to a lower quality of life and dysfunction of the sensory, motor, and autonomic systems, and often causes patients to discontinue chemotherapy.... Show more »

CHEMOTHERAPY-INDUCED NEUROPATHIC PAIN

Background

Chemotherapy-induced neuropathic pain is one of the most serious complications associated with anticancer
drugs. It leads to a lower quality of life and dysfunction of the sensory, motor, and autonomic systems, and often causes patients to discontinue chemotherapy. Chemotherapy-induced neuropathic pain is usually misdiagnosed and undertreated, due to a lack of consensus and unclear pathophysiology, for which many mechanisms have been suggested, including mitochondrial dysfunction and various pain mediators.

To date, no agents have been shown to effectively prevent chemotherapy-induced neuropathic pain, and long-term management of pain is therefore becoming one of the most challenging aspects of treatment for neurologists and oncologists.

Pathology Model

In order to recreate in vitro the chemotherapy induced neuropathic pain model, we will challenge the primary sensory neurons (Primary cultures of DRG neurons from Sprague Dawley rats) with a well-known chemotherapeutic agent, namely vincristine.

Readouts

The following parameters will be taken into consideration:

  • Cell viability
  • Axonal degeneration
  • LDH release
  • IL1beta/TNF-alpha production
  • Morphological modulation
  • Electrophysiological properties

DIABETIC NEUROPATHY

Background

Diabetic painful neuropathy affects over 40% of adult diabetic patients. The pathology has been
associated with a number of modifiable and non-modifiable risk factors, including the degree of
hyperglycemia, lipid and blood pressure indexes, diabetes duration, and height. Diabetic neuropathy
affects all peripheral nerves including pain fibers, motor neurons and the autonomic nervous system.
With the exception of tight glucose control, there are no specific treatments for diabetic neuropathy, and
current therapeutical strategies are rather aimed at reducing pain and other symptoms (Options for
pain control include tricyclic antidepressants (TCAs), serotonin-norepinephrine reuptake inhibitors
(SNRIs), antiepileptic drugs (AEDs), etc.).

Pathology Model

Primary cultures of DRG neurons, harvested from normal Sprague Dawley rats, will be cultured in
presence of high concentration of glucose (e.g. 60mM) in order to recreate diabetic condition in vitro.
Increasing evidence indicates that one of the major causes of diabetic peripheral neuropathy is an overproduction
of reactive oxygen species which leads to oxidative stress, mitochondrial dysfunction,
neuronal damage and finally apoptosis.
Neurons isolated from dorsal root ganglia (DRG) of mammals such as rodents and hamsters rendered
diabetic by treatment with drugs such as streptozotocin and alloxan have been used for study of diabetic
neuropathy.. Primary culture of DRG neurons from normal untreated rodents are now the preferred in
vitro model given they mimic events occurring in vivo and permit detailed molecular analysis.
Furthermore the use of primary cultures of DRG neurons has been adopted to obtain conspicuous data
relative to changes in morphology such as reduction in neurite extension, changes in the activity of
enzymes involved in the tricarboxylic acid cycle, electron transport chain, antioxidant systems and
molecular events involved in mitochondrial dysfunction leading to apoptosis. DRG primary cultures are
also convenient systems to trace the time kinetics of the molecular events occurring during cell death,
due to oxidative stress. Most of these events have been shown to peak between 1-3 h after treatment
with glucose due to sudden increase in ROS and associated stress. These observations concur with in
vivo studies.

Readouts

The following assays will be performed:

  • Cytoskeletal disruption
  • Morphological Analysis- structural test in high content automated microscopy
  • Functional analysis - functional test on neuronal network electrophysiological activity (MEA
    system)
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Rat
Myelination Animal Models
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Oligodendrocyte precursor cells will be isolated from glal feeder layer, then maturation will be induced by selected medium exposure. Oligodendrocytes maturation will be quantified of MPB-positive oligodendrocytes expressing O4. Myelination will be quantified by counting the number of myelinating MBP-positive oligodendrocytes as... Show more »

Oligodendrocyte precursor cells will be isolated from glal feeder layer, then maturation will be induced by selected medium exposure. Oligodendrocytes maturation will be quantified of MPB-positive oligodendrocytes expressing O4. Myelination will be quantified by counting the number of myelinating MBP-positive oligodendrocytes as a percentage of the total number of MBP-positive oligodendrocytes.

10nM Adrenomedulline or 1microM Benzatropine will be used as positive controls

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Mouse
Rat
vc
In vitro Neurotoxicity Testing
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In vitro Cardiovascular Models
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Both cardiogenic as well as septic shock in vitro models available with primary cells

Both cardiogenic as well as septic shock in vitro models available with primary cells

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Mouse
Rat
Artificial Synapse Formation Assay
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Neuronal Assays
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MicroRNA (miRNA) Synthesis
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Stem Cell Assays
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Cell Invasion Assays
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RNA Sequencing
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ILLUMINA based platform

ILLUMINA based platform

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Endothelial Barrier Screening Platform
Price on request

Background
The key mechanism of endothelial dysfunction is the imbalance of endothelium-derived nitric oxide (NO) production and reactive oxygen species (ROS) generation, resulting in a decline in the bioavailability of NO and excessive accumulation of ROS. This finally leads to oxidative stress and cellular injuries.... Show more »

Background
The key mechanism of endothelial dysfunction is the imbalance of endothelium-derived nitric oxide (NO) production and reactive oxygen species (ROS) generation, resulting in a decline in the bioavailability of NO and excessive accumulation of ROS. This finally leads to oxidative stress and cellular injuries.

Pathology Model
Endothelial cells (i.e. Human Umbilical Vein Endothelial Cells HUVEC-2 or aortic endothelial cells HAEC) will be exposed to metabolic stress (i.e. oxygen glucose deprivation) in the presence/absence of the CLIENT’s compound.

Both static and dynamic in vitro microfluidic models will be taken into consideration in order to evaluate endothelial barrier integrity.

Readouts
The following quantitative parameters will be taken into consideration:
1. Molecular Biology
Gene expression profile: endothelial cells or aortic endothelial cells will be cultured in vitro and tested for their angiogenetic properties in the presence/absence of CLIENT’s compound. Ranibizumab will be used as control. The expression of main angiogenic molecules will be quantitatively evaluated.

  1. Biochemical Characterization
    a. Total ROS production (i.e. DCF-DA fluorescent assay)
    b. NADPH-dependent superoxide formation ( i.e. Dihydroethidium (DHE) staining)
    c. UV induced DNA damage: Human RPE cells will be exposed to selected oxidative stress models (UV light irradiation) in the presence/absence of CLIENT’s compounds, and morphological, biochemical, molecular as well as functional parameters will be quantitatively measured.
    d. Cell viability and toxicity: (i.e. MTT assay)
    e. Mitochondrial damage (i.e HCS Mitochondrial Health assay)
    f. Inflammatory profile. A detailed analysis of pro inflammatory and angiogenic factor production will be characterized (i.e. IL-1α, IL-1β, IL-2, IL-4, IL-6, IL-8, IL-10, VEGF, TNFα, IFN-γ, EGF, MCP-1 etc…).
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Blood Brain Barrier Screening Platform
Price on request

Background
The Blood-Brain Barrier (BBB) is a highly selective permeability barrier that separates the circulating blood from the brain extracellular fluid in the central nervous system (CNS). The Blood-Brain Barrier allows the passage of water, some gases, and lipid soluble molecules by passive diffusion, as well as the... Show more »

Background
The Blood-Brain Barrier (BBB) is a highly selective permeability barrier that separates the circulating blood from the brain extracellular fluid in the central nervous system (CNS). The Blood-Brain Barrier allows the passage of water, some gases, and lipid soluble molecules by passive diffusion, as well as the selective transport of molecules such as glucose and amino acids that are crucial to neural function
Chronic inflammatory states, as those typically occurring in aging diseases, lead to tissue degeneration and membrane permeability thus favouring immune cell crosstalk within the central nervous system. This crosstalk is crucial in the onset of neuroinflammatory events which characterize the early steps of neuronal degeneration

Pathology Model
A model of in vitro Blood-Brain Barrier (BBB) using primary rat brain endothelial cells will be used (Maria Deli ref.). BBB will be subjected to oxygen glucose deprivation (OGD) to mimic stroke conditions and beside ROS, cell viability and eNOS functionality, BBB integrity will be evaluated.

Readouts
The following morphological, biochemical and functional parameters will be quantitatively assayed:
1. Biochemical Characterization
a. Cell viability and toxicity: (i.e. MTT assay)
b. Mitochondrial damage (i.e. HCS Mitochondrial Health assay)
c. Total ROS production (i.e. DCF-DA fluorescent assay)
d. NADPH-dependent superoxide formation (i.e. Dihydroethidium (DHE) staining).
e. Inflammatory profile: a detailed analysis of pro inflammatory and angiogenic factor production will be characterized (i.e. IL-1α, IL-1β, IL-2, IL-4, IL-6, IL-8, IL10, VEGF, TNF-α, IFN-γ, EGF, MCP-1 etc…).

  1. Morphological Characterization
    a. The morphological analysis of membrane integrity will be monitored by means of confocal microscopy on immunofluorescent-labeled cells; zona occludens and tight junction will be quantitatively evaluated.
  2. Functional Characterization
    a. BBB permeability: i.e. FITC-dextran tracer.
    b. Trans-endothelial electric resistance.

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Multiple Sclerosis Screening Platform
Price on request

Background
One of the many open questions in multiple sclerosis research is whether inflammation in the CNS is initiated by an autoimmune attack, triggered by unidentified environmental factors, or represents a response to axonal degeneration and myelin degradation secondary to processes that are intrinsic to the CNS. Lesions... Show more »

Background
One of the many open questions in multiple sclerosis research is whether inflammation in the CNS is initiated by an autoimmune attack, triggered by unidentified environmental factors, or represents a response to axonal degeneration and myelin degradation secondary to processes that are intrinsic to the CNS. Lesions characterized by microglial activation and hypoxia-like characteristics, as well as cortical lesions and the slowly progressive chronic phase of the disease, are likely driven by activated myeloid cells.
However, at present it is not clear what keeps the microglial cells activated. It is possible that the T cells found throughout the CNS of patients with multiple sclerosis provide constant stimuli, i.e. by pro-inflammatory cytokines, which activate microglia.

Pathology Platform
In order to recreate an in vitro pathological scenario mimicking MS condition, microglia cells will be exposed to detrimental challenges known to activate microglia in the neuroinflammatory scenarios leading to MS (i.e. hypoxia and LPS exposure) and functional parameters will be quantitatively evaluated.
Moreover, the effect of the CLIENT’s compound in the modulation of the detrimental scenario will be quantitatively monitored in a dose response fashion.

Readouts
The following parameters will be quantitatively evaluated:
1. Biochemical Characterization
a. Cell viability and toxicity: (i.e. MTT assay)
b. Cell migration: (i.e. chemotactic chamber)
c. Total ROS production: (i.e. DCF-DA fluorescent assay)
d. Phagocytic activity: (i.e. Vybrant Assay)
e. Mitochondrial damage: (i.e. HCS Mitochondrial Health assay)
f. Cytokine production by inflammatory panel on multiplex ELISA: (i.e. IL-1α , IL-1β, IL-2, IL-4, IL-6, IL-8, IL-10, VEGF, TNF-α, IFN-γ, EGF, MCP-1)
g. NO production: (i.e. Griess assay)

  1. Functional Characterization
    a. Membrane permeability (i.e. Yo-Pro1 uptake)
    b. Microvesicle shedding
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Cerebral Ischemia Screening Platform
Price on request

Pathology model
Primary cortical neurons will be exposed to oxygen glucose deprivation (OGD) for 1h in presence absence of CLIENT’s compound and to reoxygenation for 24 hours as a model of cerebral ischemia.
Complicating the biological scenario, as cerebral microenvironment plays a crucial role in pathogenesis of cerebral... Show more »

Pathology model
Primary cortical neurons will be exposed to oxygen glucose deprivation (OGD) for 1h in presence absence of CLIENT’s compound and to reoxygenation for 24 hours as a model of cerebral ischemia.
Complicating the biological scenario, as cerebral microenvironment plays a crucial role in pathogenesis of cerebral ischemia, neurons will be put in microfluidic communication with glia from different brain areas (such as cortex or hippocampus) and then subjected to OGD and reoxygenation following the protocol described above, in presence absence of CLIENT’s compound.

Readouts
After reoxygenation the following parameters will be evaluated:
Step 1 - Direct Neuronal Damage:
1. Morphological characterization
a. Qualitative evaluation of neuronal cytoskeletal disruption
b. Quantitative evaluation of dendritic branching
c. Quantitative evaluation of neurite elongation modulation
2. Biochemical characterization
a. Quantitative evaluation of neuronal cell death (i.e. PI/DAPI/Calcein AM)
b. Quantitative evaluation of caspase activation (i.e. 3 or 8 or 9)
c. Quantitative evaluation of DNA degradation (i.e. TUNEL staining)
3. Analysis of oxidative stress
a. Quantitative evaluation of total ROS production
b. Quantitative evaluation of NO production
c. Mitochondrial membrane potential (MMP) (JC-10 dye by flow cytometry)
Step 2 - Glial Pro-Inflammatory Phenotype:
1. Biochemical characterization
a. Quantitative evaluation of metabolic activity
b. Inflammatory cytokine production: (i.e. IL1 beta, TNFalpha, IL6)
c. Total ROS production
d. Quantification of NO production
2. Functional characterization
a. Quantitative evaluation of phagocytic potential
b. Quantitative evaluation of membrane permeability
c. Quantitative evaluation of microvesicle shedding

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Neuropathic Pain Screening Platform
Price on request

Background
Neuropathic pain is caused by damage or disease that affects the somatosensory system. Central neuropathic pain is found in spinal cord injury, multiple sclerosis, and some strokes. Central sensitization is an important mechanism of persistent neuropathic pain.

Pathology Model
The modulatory effect of the CLIENT’s... Show more »

Background
Neuropathic pain is caused by damage or disease that affects the somatosensory system. Central neuropathic pain is found in spinal cord injury, multiple sclerosis, and some strokes. Central sensitization is an important mechanism of persistent neuropathic pain.

Pathology Model
The modulatory effect of the CLIENT’s compound on vincristine challenged trigeminal gangliar neurons will be monitored by functional and morphological parameters. Particular attention will be given to neurite outgrowth assay, performed with a well established model of dissociated primary trigeminal sensory ganglia neurons (TG) in culture. Ganglia from young (P12) C57-Black mice will be dissociated and plated in a series of bottom imaging black 96well plates and kept in culture. At 24h after plating, cells will be pre exposed for to the CLIENT’s compound and then cells will be exposed to damaging action exerted by vincristine at predefined concentration.
At a pre-determined time after compound exposure, cells will be fixed and immuno-stained with antibodies against specific neuronal cytoskeleton marker beta-Tubulin3. Cells will be counterstained with Hoechst 33342 (Invitrogen) prior to image acquisition. The use of specific immunostaining for neuronal cytoskeleton filters neuronal process from the mixed cell populations, so neurite analysis is performed only on neurons. The efficacy of neuro-protective action exerted by the compound will be quantitatively monitored

Readouts
Overall, assay that will be taken into consideration are:
1. Biochemical Characterization
a. Evaluation of neuronal viability
b. Evaluation of total ROS production
c. Evaluation of LDH release

  1. Morphological Evaluation
    a. Neurite outgrowth assay
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Parkinson's Disease Screening Platform
Price on request

Background
The precise cause of Parkinson’s Disease (PD) is unknown, but there is a consensus that an inflammatory event is involved in the initiation of neurodegeneration, and that chronic neuroinflammation is a sustaining and exacerbating reason for the loss of the dopaminergic neurons.
Recent findings have revealed that the... Show more »

Background
The precise cause of Parkinson’s Disease (PD) is unknown, but there is a consensus that an inflammatory event is involved in the initiation of neurodegeneration, and that chronic neuroinflammation is a sustaining and exacerbating reason for the loss of the dopaminergic neurons.
Recent findings have revealed that the functional interaction between astrocytes, microglia and neurons govern both the sequence of inflammatory events (i.e. cascades of inflammatory mediators) and the pathological outcome (damage or absence of damage) to neurons. Among the proinflammatory molecules, cytokines play a central role in the self-propagation of neuroinflammation in PD.
In spite of the evidence indicating that inflammation might influence the pathogenesis of PD, there is considerable debate concerning which molecules are synthesized and released, how astrocytes and microglia interact reciprocally and with neuronal cells within the neurovascular unit, and how the kinetic responses and the precise connectivity of the inflammatory cascades are regulated

Pathology Model

In order to evaluate the CLIENT’s compound modulatory activity of the neuroinflammatory events leading to dopaminergic neuron degeneration, physiologically relevant cell cultures of dopaminergic (DA) neurons will be either directly intoxicated with 6-OHDA or exposed to 6-OHDA primed glial medium. Both the direct effects on dopaminergic neurons as well as the microglial-mediated effects will be taken into consideration.

Readouts

The following parameters will be analyzed:
Step 1 - Direct Neuronal Damage:

  1. Morphological characterization
    a. Qualitative evaluation of neuronal cytoskeletal disruption
    b. Quantitative evaluation of dendritic branching
    c. Quantitative evaluation of neurite elongation modulation

  2. Biochemical characterization
    a. Quantitative evaluation of neuronal cell death (i.e. PI/DAPI/Calcein AM)
    b. Quantitative evaluation of caspase activation (i.e. 3 or 8 or 9)
    c. Quantitative evaluation of DNA degradation (i.e. TUNEL staining)

  3. Analysis of oxidative stress
    a. Quantitative evaluation of total ROS production
    b. Quantitative evaluation of NO production

Step 2 - Glial Pro-Inflammatory Phenotype:

  1. Biochemical Characterization

a. Quantitative evaluation of metabolic activity
b. Inflammatory cytokine production: (i.e. IL1 beta, TNFalpha, IL6)
c. Total ROS production
d. Quantification of NO production

  1. Functional Characterization

a. Quantitative evaluation of phagocytic potential
b. Quantitative evaluation of membrane permeability
c. Quantitative evaluation of microvesicle shedding

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Alzheimer's Disease Screening Platform
Price on request

Background

Alzheimer’s disease (AD) is the most common cause of dementia among ageing population. However, the mechanisms regulating synaptic dysfunction in AD are not fully understood, and require an in depth analysis of the crosstalk mechanisms ongoing during an inflammatory event, between the neuronal and non-neuronal cells... Show more »

Background

Alzheimer’s disease (AD) is the most common cause of dementia among ageing population. However, the mechanisms regulating synaptic dysfunction in AD are not fully understood, and require an in depth analysis of the crosstalk mechanisms ongoing during an inflammatory event, between the neuronal and non-neuronal cells present in the microenvironment, playing a crucial role in disease onset.

Pathology Model

In order to evaluate the CLIENT’s compound modulatory activity of the neuroinflammatory events leading to neurdegeneration in AD, hippocampal neurons will be exposed to either Abeta oligomers, or to glial Abeta- primed glial medium. Both the direct effects on hippocampal neurons as well as the microglial-mediated effects will be taken into consideration.

Readouts

The following parameters will be analyzed:

Step 1 - Direct Neuronal Damage:
Primary neuronal cultures isolated from Sprague Dowley rats will be exposed to Abeta oligomers in the presence / absence of CLIENT’s. The following parameters will be analyzed:
1. Morphological Characterization
a. Qualitative evaluation of neuronal cytoskeletal disruption
b. Quantitative evaluation of dendritic branching
c. Quantitative evaluation of neurite elongation modulation

  1. Biochemical Characterization
    a. Quantitative evaluation of neuronal cell death (i.e. PI/DAPI/Calcein AM)
    b. Quantitative evaluation of caspase activation (i.e. 3 or 8 or 9)
    c. Quantitative evaluation of DNA degradation (i.e. TUNEL staining)

  2. Analysis of Oxidative Stress
    a. Quantitative evaluation of total ROS production
    b. Quantitative evaluation of NO production

Step 2 - Glial Pro-Inflammatory Phenotype:

Microglia cells (i.e. BV2) will be challenged with Abeta oligomers in the presence/absence of CLIENT’s compound. The following parameters will be quantitatively monitored on glial cells:

  1. Biochemical characterization
    a. Quantitative evaluation of metabolic activity
    b. Inflammatory cytokine production: (i.e. IL1 beta, TNFalpha, IL6)
    c. Total ROS production
    d. Quantification of NO production

  2. Functional characterization

a. Quantitative evaluation of phagocytic potential
b. Quantitative evaluation of intracellular calcium dynamics
c. Quantitative evaluation of membrane permeability
d. Quantitative evaluation of microvesicle shedding

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Adhesion Molecules Detection Assay
Price on request

Immunoassay testing platform for the simultaneous multi-detection of 5 soluble adhesion molecules in a single very low volume sample (2.5µl).
• E-Selectin
• P-Selectin
• L-Selectin
• Intercellular Adhesion Molecule-1 (ICAM-1)
• Vascular Cell Adhesion Molecule-1 (VCAM-1)

Immunoassay testing platform for the simultaneous multi-detection of 5 soluble adhesion molecules in a single very low volume sample (2.5µl).
• E-Selectin
• P-Selectin
• L-Selectin
• Intercellular Adhesion Molecule-1 (ICAM-1)
• Vascular Cell Adhesion Molecule-1 (VCAM-1)

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Animal Behavior Studies
Price on request

We offer a comprehensive, multi-step characterization of the anatomical, physiological, and behavioral changes in your newly created genetically engineered models.
Panels generally incorporate:
• In-life analysis with clinical examinations, primary behavioral observations, growth curves, reproductive analysis, and cognitive... Show more »

We offer a comprehensive, multi-step characterization of the anatomical, physiological, and behavioral changes in your newly created genetically engineered models.
Panels generally incorporate:
• In-life analysis with clinical examinations, primary behavioral observations, growth curves, reproductive analysis, and cognitive analysis
• Pathology services in support of phenotypic characterization with clinical pathology, necropsy evaluations, and histopathology
• Molecular phenotyping assays utilizing quantitative PCR (Q-PCR) techniques,
• Biomarker screening via Multi-Analyte Profiles (MAP)

We can breed and maintain your genetically engineered colonies and readily transfer the offspring to our phenotyping program. Alternatively, we can receive animals for phenotyping directly from your facility.

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Cell Migration Assays
Price on request

We provide the following cell migration assays:
- Boyden chamber assay and multiwell migration systems
- Microfluidics technology-based assay

We provide the following cell migration assays:
- Boyden chamber assay and multiwell migration systems
- Microfluidics technology-based assay

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Cell Proliferation Assays
Price on request

We provide evaluation of cell’s clonigenic performance, cell’s DNA synthesis and cell’s metabolic activity through:
- Membrane staining (cristal violet, alamar blu, ecc)
- Fluorescent-based assay
- BrdU staining
- Fluorescence/luminescence assay (BodiPy dUTP)
- MTT assay, etc…

We provide evaluation of cell’s clonigenic performance, cell’s DNA synthesis and cell’s metabolic activity through:
- Membrane staining (cristal violet, alamar blu, ecc)
- Fluorescent-based assay
- BrdU staining
- Fluorescence/luminescence assay (BodiPy dUTP)
- MTT assay, etc…

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Apoptosis Assays
Price on request

We provide evaluation of plasma-membrane permeability and DNA fragmentation and cell’s metabolic activity through:
- Nuclear staining (propidium iodide, tunel)
- Electrochemical gradient across mitochondrial membrane
- Cytocrome C release
- Annexin V
- Fluorescent evaluation of phosphatidyserine
- Protease activity assay (caspase 1,3,8)
- Substrate for peptidase/caspase

We provide evaluation of plasma-membrane permeability and DNA fragmentation and cell’s metabolic activity through:
- Nuclear staining (propidium iodide, tunel)
- Electrochemical gradient across mitochondrial membrane
- Cytocrome C release
- Annexin V
- Fluorescent evaluation of phosphatidyserine
- Protease activity assay (caspase 1,3,8)
- Substrate for peptidase/caspase

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Transfection
Price on request

We offer customized cloning DNA, siRNA, protocol design and implementation support and evaluation of phenotypic changes in transiently transfected cells

We offer customized cloning DNA, siRNA, protocol design and implementation support and evaluation of phenotypic changes in transiently transfected cells

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Biomarker Analysis
Price on request

Cerebral Biomarker Detection Assay

Immunoassay testing platform for the simultaneous multi-detection of 5 cerebral biomarkers in a very low volume sample (35-100µl) with excellent sensitivity, precision and recovery.
The assays can be performed in two specific cerebral array:
Cerebral Array... Show more »

Cerebral Biomarker Detection Assay

Immunoassay testing platform for the simultaneous multi-detection of 5 cerebral biomarkers in a very low volume sample (35-100µl) with excellent sensitivity, precision and recovery.
The assays can be performed in two specific cerebral array:
Cerebral Array I:
• BDNF
• hFABP
• GFAP
• IL-6
Cerebral Array II:
• NSE
• NGAL
• sTNFRI
• D-dimer
• CRP

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Compound Profiling
Price on request

We provide customized solutions to unfold cellular complexity using a proprietary microfluidics technology, WIDE BioSCREEN™ (WBS).
WBS is an innovative multi-parametric platform for unfold cell-to-cell communication on in complex intercellular scenarios (brain, cancer, immune system, blood,liver, etc…). The technology recreates... Show more »

We provide customized solutions to unfold cellular complexity using a proprietary microfluidics technology, WIDE BioSCREEN™ (WBS).
WBS is an innovative multi-parametric platform for unfold cell-to-cell communication on in complex intercellular scenarios (brain, cancer, immune system, blood,liver, etc…). The technology recreates tissue/disease-like environments in vitro enabling a parallel multi-parametric analysis of the cellular response, taking into consideration a wide range of parameters which characterize the biological activity of the cells.
WIDE BioSCREEN™ enables early availability of highly predictive information on toxicology and effectiveness, a more informative biological candidates screenings, an improved lead optimization and validation process, a deep understanding of the functional role of surrounding cells in the biological effects candidate’s mediated and the dissection of molecular mechanisms involved downstream target receptor activation.

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Cytokine Analysis
Price on request

Testing platform for the simultaneous multi-analyte testing in normal and High Sensibility mode. The combination of highly specific antibodies and advanced chemistries enables up to 12 cytokines and growth factors to be detected simultaneously in a single very low volume sample (8.3µl).

Cytokine Immunoassay
Immunoassay... Show more »

Testing platform for the simultaneous multi-analyte testing in normal and High Sensibility mode. The combination of highly specific antibodies and advanced chemistries enables up to 12 cytokines and growth factors to be detected simultaneously in a single very low volume sample (8.3µl).

Cytokine Immunoassay
Immunoassay testing platform for the simultaneous multi-analyte testing in normal and High Sensibility mode. The combination of highly specific antibodies and advanced chemistries enables up to 12 cytokines and growth factors to be detected simultaneously in a single very low volume sample (8.3µl).
The assays can be performed in several specific cytokine array:
Array I:
• Interleukin-1 α (IL-1α)
• Interleukin-1 β (IL-1β)
• Interleukin-2 (IL-2)
• Interleukin-4 (IL-4)
• Interleukin-6 (IL-6)
• Interleukin-8 (IL-8)
• Interleukin-10 (IL-10)
• Epidermal Growth Factor (EGF)
• Interferon-γ (IFN-γ)
• Monocyte Chemotactic Protein-1 (MCP-1)
• Tumor Necrosis Factor-α (TNF-α)
• Vascular Endothelial Growth Factor (VEGF)
Array II:
• Platelet Derived Growth Factor AA (PDGF-AA)
• Insulin-like Growth Factor 1 free (IGF-1)
• RANTES
• Eotaxin
• Interleukin 1 receptor antagonist (IL-1 Ra)
• Platelet Derived Growth Factor BB (PDGF-BB)
• Interferon inducible protein - 10 (IP-10)
• Interleukin 12p40 (IL12-p40)
Array III:
• Interleukin-5 (IL-5)
• Interleukin-15 (IL-15)
• Granulocyte Macrophage Colony Stimulating Factor (GM-CSF)
• Macrophage Inflammatory Protein - (1α MIP-1α)
Array IV:
• Matrix Metalloproteinase-9 (MMP-9)
• Soluble IL-2 Receptor α (sIL-2Rα)
• Soluble IL-6 Receptor (sIL-6R)
• Soluble Tumour Necrosis Factor Receptor I (sTNFRI)
• Soluble Tumour Necrosis Factor Receptor II (sTNFRII)
Array V:
• Interleukin- 3 (IL-3)
• Interleukin- 7 (IL-7)
• Interleukin- 13 (IL-13)
• Interleukin- 12 p70 (IL-12 p70)
• Interleukin- 23 (IL-23)

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ELISA
Enzyme-linked immunosorbent assay
Price on request

Capabilities:
- Kit-based
- Sandwich, Multiplex, ELISpot, in-Cell ELISA (ICE)
- Homogenization and sonication of cell/tissue lysates
- Transfection/Transduction of mammalian
- Analyze serum, plasma, cells, tissues
- Analyze fluorescent/chemiluminescent/chromogenic substrates

Equipment:
- LI-Cor Odissey (In-Cell... Show more »

Capabilities:
- Kit-based
- Sandwich, Multiplex, ELISpot, in-Cell ELISA (ICE)
- Homogenization and sonication of cell/tissue lysates
- Transfection/Transduction of mammalian
- Analyze serum, plasma, cells, tissues
- Analyze fluorescent/chemiluminescent/chromogenic substrates

Equipment:
- LI-Cor Odissey (In-Cell ELISA)
- Ciraplex
- Multi-channel, and automatic pipets

Number of samples processed in a single experiment: 50 in duplicate

Typical turn-around time: 30 days

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Genotyping Services
Price on request
Request a quote for more information about this service.
Immunocytochemistry
Price on request

We provide general staining, membrane staining, nucleic acid staining, organelle and protein staining and immunocytochemistry for:
- Viability and proliferation
- Customized analysis of protein expression and localization in normal or treated conditions
- Customized sections prepared from specific animal models either... Show more »

We provide general staining, membrane staining, nucleic acid staining, organelle and protein staining and immunocytochemistry for:
- Viability and proliferation
- Customized analysis of protein expression and localization in normal or treated conditions
- Customized sections prepared from specific animal models either challenged/unchallenged with specific pharmacological treatments (brain, heart, intestine (small), intestine (large), kidney, liver, lungs, ovary, spleen…)

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In vitro Toxicity Testing
Price on request

We provide in vitro viability and toxicity assays for quantitative evaluation of cell’s death and cell’s vitality metabolism through:
- Nuclear Staining (Trypan blue/ P.I/ Hoechst/ YO-PRO)
- Cellular substance release (i.e. LDH assay)
- Bioluminescent assay (ATP based luciferase reaction)
- Esterase substrate (i.e.... Show more »

We provide in vitro viability and toxicity assays for quantitative evaluation of cell’s death and cell’s vitality metabolism through:
- Nuclear Staining (Trypan blue/ P.I/ Hoechst/ YO-PRO)
- Cellular substance release (i.e. LDH assay)
- Bioluminescent assay (ATP based luciferase reaction)
- Esterase substrate (i.e. Calcein-AM)
- MTT assay
- Glucose Uptake
- Measurement of oxidation/reduction state

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In vitro Neurodegeneration Models
Price on request

We can provide in vitro models for several pathologic conditions:
- BROAD SPECTRUM:
o Cytoskeletal alterations via immunocytochemistry
o Alteration of synaptic plasticity
o Tau phosphosylation analysis
o Synapsin dispersion
o Glutamatergic/GABAergic cell specific contribution
o Ionomycin/Bapta... Show more »

We can provide in vitro models for several pathologic conditions:
- BROAD SPECTRUM:
o Cytoskeletal alterations via immunocytochemistry
o Alteration of synaptic plasticity
o Tau phosphosylation analysis
o Synapsin dispersion
o Glutamatergic/GABAergic cell specific contribution
o Ionomycin/Bapta induced alteration of calcium equilibrium

  • EPILEPSY:
    o Glutamate-induced excitotoxicity in vitro model
    o Kainic acid-induced excitotoxicity in vitro model

  • ISCHEMIA:
    o Oxygen -glucose deprivation in vitro model

  • ALZHEIMER:
    o In vitro exposure to Ab fibrils

  • PARKINSON:
    o MPP+ challenge of neurons
    o Tyrosine OH staining

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In vitro Inflammation Models
Price on request
Request a quote for more information about this service.
Mammalian Cell Culture
Price on request

We provide Rat, Mouse and Human cell cultures in different formats (cryovials, homogenates, mRNA extracts, fixed cells).

BRAIN:
Rat and Mouse
- Primary cortical astrocytes
- Primary hippocampal astrocytes
- Primary cortical neurons
- Primary hippocampal neurons
- Primary microglia
- Primary DRG cultures
- Primary... Show more »

We provide Rat, Mouse and Human cell cultures in different formats (cryovials, homogenates, mRNA extracts, fixed cells).

BRAIN:
Rat and Mouse
- Primary cortical astrocytes
- Primary hippocampal astrocytes
- Primary cortical neurons
- Primary hippocampal neurons
- Primary microglia
- Primary DRG cultures
- Primary Subventricular Zone
Human
- Glioma
- Glioblastoma

CANCER:
Human
- Neuroblastoma
- Rhabdomyosarcoma
- Pancreatic Adenocarcinoma
- Anaplastic Large Cell Lymphoma

IMMUNE SYSTEM:
- Human Monocytes

LIVER:
- Human Hepatocytes

STEM CELL:
Rat, Mouse and Human
- Mesenchymal (Adipose Tissue-derived MSC and Bone Marrow-derived MSC)
- Neural Stem Cells (Human Glioblastoma, Skin)

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Pathology Microenvironment Analysis
Price on request

We have developed a multiparametric microfluidic platform (WIDEBioSCREEN) for unfolding cell-cell communication in complex intercellular scenarios ( CNS, oncology, rheumatoid arthitis, immune diseases, metabolic diseases, etc. ) There are many advantages associated with the use of this platform:
• tissue complexity is recreated ... Show more »

We have developed a multiparametric microfluidic platform (WIDEBioSCREEN) for unfolding cell-cell communication in complex intercellular scenarios ( CNS, oncology, rheumatoid arthitis, immune diseases, metabolic diseases, etc. ) There are many advantages associated with the use of this platform:
• tissue complexity is recreated and finely modulated in-vitro
• cell specific contribution in a pathophysiological context is dissected
• compensatory intracellular mechanisms are identified
• experiments are run on microscale (with very limited cell samples) and on primary cell lines

The use of WIDEBioSCREEN yields main economic benefits at different stages of drug development:
• R&D: enables rapid and informative comprehension of molecular mechanisms associated with response to candidate drug
• Preclinical : enables effective selection and ranking of the most promising candidates per indication
• Repositioning: enables cost effective repurposing of failed candidates through recreation in vitro of tissues complexity and identification of new indications.

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Preclinical PK/PD Studies
in vivo Pharmacokinetics/Pharmacodynamics
Price on request

Our dedicated Pharmacokinetics group works closely with other operational departments to provide pharmacokinetic data for animalclinical studies in a timely fashion.
We can provide the following services:
• Non-compartmental pharmacokinetics
• Compartmental pharmacokinetics/simulations,
• Ascending dose (assessment of dose... Show more »

Our dedicated Pharmacokinetics group works closely with other operational departments to provide pharmacokinetic data for animalclinical studies in a timely fashion.
We can provide the following services:
• Non-compartmental pharmacokinetics
• Compartmental pharmacokinetics/simulations,
• Ascending dose (assessment of dose proportionality)
• Repeat dose (assessment of multiple dose linearity)
• Bioavailability and bioequivalence
• Drug interaction studies
• Pharmacodynamic and PK/PD modeling
• Input into study design, including preclinical-to-clinical considerations (allometric scaling) utilizing information from preclinical toxicokinetic studies
Our pharmacokinetic consultancy service provides advice and support for all aspects of clinical pharmacokinetics and is offered as part of a complete development program, as a full-service single-study package, or as a stand-alone service. Upon request, pharmacokinetic parameter estimation output and reports may include a comprehensive text interpretation of the data.

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Predictive Toxicology
Price on request

We provide customized solutions to unfold cellular complexity using a proprietary microfluidics technology, WIDE BioSCREEN™ (WBS).
WBS is an innovative multi-parametric platform for unfold cell-to-cell communication on in complex intercellular scenarios (brain, cancer, immune system, blood,liver, etc…). The technology recreates... Show more »

We provide customized solutions to unfold cellular complexity using a proprietary microfluidics technology, WIDE BioSCREEN™ (WBS).
WBS is an innovative multi-parametric platform for unfold cell-to-cell communication on in complex intercellular scenarios (brain, cancer, immune system, blood,liver, etc…). The technology recreates tissue/disease-like environments in vitro enabling a parallel multi-parametric analysis of the cellular response, taking into consideration a wide range of parameters which characterize the biological activity of the cells.
WIDE BioSCREEN™ enables early availability of highly predictive information on toxicology and effectiveness, a more informative biological candidates screenings, an improved lead optimization and validation process, a deep understanding of the functional role of surrounding cells in the biological effects candidate’s mediated and the dissection of molecular mechanisms involved downstream target receptor activation.

« Show less
Protein Purification
Price on request

We provide protein purification, sample preparation, gel electrophoresis and immune assays for:
- Customized cells protein isolation and quantification
- Subcellular organelles preparation (exosomes, microvesicles)
- Immuno precipitation
- Co-immunoprecipitation
- SDS Page
- 2D gel electrophoresis
- Protein gel... Show more »

We provide protein purification, sample preparation, gel electrophoresis and immune assays for:
- Customized cells protein isolation and quantification
- Subcellular organelles preparation (exosomes, microvesicles)
- Immuno precipitation
- Co-immunoprecipitation
- SDS Page
- 2D gel electrophoresis
- Protein gel staining
- Multi Cytokine release analysis (ELISA)
- Morphological analysis by immunocytochemical staining

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RNA Extraction
Price on request

gel extraction sequencing, quantification

gel extraction sequencing, quantification

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PAGE
Polyacrylamide Gel Electrophoresis
Price on request
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Signaling Pathway Analysis
Price on request

We provide ad hoc analysis of signalling pathways and smart data/results interpretation to unfold complex intercellular comunication.

We provide ad hoc analysis of signalling pathways and smart data/results interpretation to unfold complex intercellular comunication.

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TUNEL Assay
Terminal deoxynucleotidyl transferase dUTP nick end labeling assay
Price on request

Tunel assay for the detection of apoptotic cells in tissue sections

Tunel assay for the detection of apoptotic cells in tissue sections

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qPCR
Quantitative PCR
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siRNA Synthesis
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Immunoassays
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Cell Signaling Analyses
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Lead Identification and Validation
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Computational Modeling
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ADME/DMPK Studies
Drug Metabolism and Pharmacokinetics
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Electrophoresis
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Cells and Tissues
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Cell and Tissue Culture
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PCR
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Animal Learning, Memory, and Behavior Tests
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Specialized Cell-Based Assays
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Animal Model in vivo Analyses
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CNS/Neurology Animal Models
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Drug Discovery & Development
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Biomarkers
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Pharmacology & Toxicology
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Cell-Based Assays
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Immune Cell Assays
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DNA Damage & Repair Assays
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Cell Invasion & Migration Assays
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Protein Quantification
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Protein Services
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Toxicology
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Protein Expression Visualization
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Animal Cognition & Behavior Tests
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Purification Services
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Protein Purification and Quantification
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Cytotoxicity Assays
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Nucleic Acid Services
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DNA Services
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Animal Models and Studies
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Animal Models of Disease
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Chemistry and Materials
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Functional & Cell Type Specific Assays
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Cell Death Assays
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Biochemistry & Molecular Biology
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In vitro Disease Models
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Gel Electrophoresis
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Immunostaining
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Biology
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RNA Services
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Drug Discovery
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Cell Viability & Proliferation Assays
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Analytical Chemistry Services
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Bioanalysis
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Pain/Neuropathy Animal Models
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Pain/Neuropathy Animal Models Services

Pain/Neuropathy Animal Models Services

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Enzyme Assays
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Enzyme Assays Services

Enzyme Assays Services

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Biochemical Assays
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Biochemical Assays Services

Biochemical Assays Services

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In vivo Toxicity Testing
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In vivo Toxicity Testing Services

In vivo Toxicity Testing Services

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Cellular Health & Metabolism Assays
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Cellular Health & Metabolism Assays Services

Cellular Health & Metabolism Assays Services

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DNA Extraction and Purification
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Induced Pluripotent Stem Cell (iPSC) Culture
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RT-PCR
Reverse transcription polymerase chain reaction
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