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NeoProteomics, Inc.

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Cleveland, Ohio, US

About NeoProteomics, Inc.

NeoProteomics, Inc. offers protein structure characterization services to industry and academic laboratories. NeoProteomics’ team of world-class experts is led by Dr. Mark Chance, Chief Scientific Officer and Founder. Dr. Chance has pioneered covalent labeling mass spectrometry (CL-MS) as a fast, high... Show more »

NeoProteomics, Inc. offers protein structure characterization services to industry and academic laboratories. NeoProteomics’ team of world-class experts is led by Dr. Mark Chance, Chief Scientific Officer and Founder. Dr. Chance has pioneered covalent labeling mass spectrometry (CL-MS) as a fast, high resolution approach to protein characterization by mass spectrometry and has partnered with several major Pharmaceutical and Biotechnology companies to characterize therapeutic proteins at the side chain level, providing enhanced patentability, and have characterized antibody structure to assess conformational variability relevant to re-engineering or manufacturing process quality control.
Services Include:
• Higher Order Structural Analysis
• Epitope Mapping
• Protein-Drug Interaction Mapping
• Crystallography
• Proteomics Services
• Expert Consulting

Publications

  • Kaur, P., Kiselar, J.G., Shi, W., Yang, S., Chance, M.R. “Covalent Labeling Techniques for Characterizing Higher Order Structure of Monoclonal Antibodies”, in State of the Art and Emerging Technologies for Therapeutic Monoclonal Antibody Characterization. Volume 3. Defining the Next Generation of Analytical and Biophysical Techniques. Editors: Darryl Lee Davis, Oleg Borisov, and John Schiel, ACS Symposium Series # 1202, Chapter 3, pp 45- 73, (2015).
  • Kaur P, Tomechko SE, Kiselar J, Shi W, Deperalta G, Wecksler AT, Gokulrangan G, Ling V, Chance MR. Characterizing monoclonal antibody structure by carboxyl group footprinting. MAbs (2015) 7(3):540-52. doi: 10.1080/19420862.2015.1023683. PMID: 25933350 PMCID: PMC4622658
  • Kaur P, Kiselar J, Yang S, Chance MR. Quantitative protein topography analysis and high-resolution structure prediction using hydroxyl radical labeling and tandem-ion mass spectrometry (MS). Molecular & cellular proteomics : MCP. (2015);14(4):1159-68. doi:10.1074/mcp.O114.044362. PubMed PMID: 25687570; PubMed Central PMCID:PMC4390260.
  • Huang W, Ravikumar KM, Chance MR, Yang S. “Quantitative mapping of protein structure by hydroxyl radical footprinting-mediated structural mass spectrometry: a protection factor analysis”. Biophys J (2015); 108(1):107-15. doi: 10.1016/j.bpj.2014.11.013. PMCID: PMC4286602
  • Kaur, P.; Kiselar, J.; Shi, W.; Chance, M. R.; Covalent Labeling Techniques for Characterizing Higher Order Structure of Monoclonal Antibodies, Chapter 21, Current, State of the Art, and Emerging Technologies for the Analysis of Monoclonal Antibodies, ACS Books (2014)
  • Kaur, P., Chance, M.R., Chapter 21: “The Utility of Mass Spectrometry Based Structural Proteomics in Biopharmaceutical Biologics Development”, Integrative Proteomics, ISBN 978-953- 51-0070-6, H-C Leung, Editor, In-Tech, p.395-412, (2012).
  • Xu, G., Chance, M.R., Hydroxyl radical-mediated modification of proteins as probes for structural proteomics, Chem Rev. (2007) Aug;107(8):3514-43. PMID: 17683160 DOI: 10.1021/cr0682047
  • Takamoto, K., Chance, M.R., Radiolytic protein footprinting with mass spectrometry to probe the structure of macromolecular complexes, Annu Rev Biophys Biomol Struct. (2006);35:251-76. DOI: 10.1146/annurev.biophys.35.040405.102050
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Our Services (36)


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X-ray Crystallography

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Epitope Mapping

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NeoProteomics, Inc. offers covalent labeling (CL) and mass spectrometry (MS) services to industry to characterize therapeutic proteins. These services precisely defined epitopes for antigen targets and provide structural characterization of therapeutic proteins suitable for biosimilarity comparisons or any relevant structural... Show more »

NeoProteomics, Inc. offers covalent labeling (CL) and mass spectrometry (MS) services to industry to characterize therapeutic proteins. These services precisely defined epitopes for antigen targets and provide structural characterization of therapeutic proteins suitable for biosimilarity comparisons or any relevant structural definition of the product. Specifically CL-MS (often called protein footprinting) provides a high resolution and sensitive readout of the side chain environment (e.g. solvent accessibility) for virtually all side chains of a protein of interest. Changes in solvent accessibility of side chains, due to ligand binding (e.g. epitope mapping), sequence or process variation, or changes in solvent are easily read out by the technology and are immediately structurally interpretable.

Dr. Mark Chance, NeoProteomics Chief Scientific Officer and Founder, pioneered CL-MS as a fast, high resolution approach to protein characterization by mass spectrometry and has partnered with several major Pharmaceutical and Biotechnology companies to characterize epitopes at the side chain level, providing enhanced patentability, and have characterized antibody structure to assess conformational variability relevant to re-engineering or manufacturing process variation.

Competing technologies for protein characterization, like hydrogen-deuterium exchange (HDX) or NMR may be suitable for assessing conformation variability and/or epitopes. NMR, depending on the technique, (1-D, 2-D or 3-D) may lack specificity or sensitivity, or be prohibitive in cost. HDX is a powerful approach to examining backbone stability, but is more challenging for larger proteins, proteins that have multiple disulfide bonds or proteins that are heavily glycosylated.

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Protein Characterization

Price on request

NeoProteomics, Inc. offers covalent labeling (CL) and mass spectrometry (MS) services to industry to characterize therapeutic proteins. These services precisely defined epitopes for antigen targets and provide structural characterization of therapeutic proteins suitable for biosimilarity comparisons or any relevant structural... Show more »

NeoProteomics, Inc. offers covalent labeling (CL) and mass spectrometry (MS) services to industry to characterize therapeutic proteins. These services precisely defined epitopes for antigen targets and provide structural characterization of therapeutic proteins suitable for biosimilarity comparisons or any relevant structural definition of the product. Specifically CL-MS (often called protein footprinting) provides a high resolution and sensitive readout of the side chain environment (e.g. solvent accessibility) for virtually all side chains of a protein of interest. Changes in solvent accessibility of side chains, due to ligand binding (e.g. epitope mapping), sequence or process variation, or changes in solvent are easily read out by the technology and are immediately structurally interpretable.

Dr. Mark Chance, NeoProteomics Chief Scientific Officer and Founder, pioneered CL-MS as a fast, high resolution approach to protein characterization by mass spectrometry and has partnered with several major Pharmaceutical and Biotechnology companies to characterize epitopes at the side chain level, providing enhanced patentability, and have characterized antibody structure to assess conformational variability relevant to re-engineering or manufacturing process variation.

Competing technologies for protein characterization, like hydrogen-deuterium exchange (HDX) or NMR may be suitable for assessing conformation variability and/or epitopes. NMR, depending on the technique, (1-D, 2-D or 3-D) may lack specificity or sensitivity, or be prohibitive in cost. HDX is a powerful approach to examining backbone stability, but is more challenging for larger proteins, proteins that have multiple disulfide bonds or proteins that are heavily glycosylated.

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Protein Crystallization Screening and Optimization

Price on request

NeoProteomics (Neo), has advanced capabilities for crystallography services. Services from crystallization trials to data collection to structural solution are available including rapid turnaround. Synchrotron-based macromolecular crystallography is one of the most important techniques to uncover the details of molecular structure... Show more »

NeoProteomics (Neo), has advanced capabilities for crystallography services. Services from crystallization trials to data collection to structural solution are available including rapid turnaround. Synchrotron-based macromolecular crystallography is one of the most important techniques to uncover the details of molecular structure at an atomic level.

NeoProteomics (Neo), in partnership with the Case Center for Synchrotron Biosciences (CSB) which has unique resources for macromolecular structure analysis at the National Synchrotron Light Source (NSLS), Brookhaven National Laboratory in New York. Two state-of-the-art beamlines operated by the CSB at the NSLS (X29 Mini-Gap Undulator and X3A Bending Magnet) are dedicated to MAD/SAD macromolecular crystallography.

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LC-MS/MS

Liquid chromatography tandem mass spectrometry
Price on request
  • Short Gradient
  • Medium Gradient
  • Long Gradient
  • Short Gradient
  • Medium Gradient
  • Long Gradient
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Protein Structure Determination

Price on request
  • Global Phosphoproteome Analysis
    • Includes digestion, enrichment, LC/MS/MS and analysis
  • Qualitative Phosphoproteomic Analysis
    • Includes digestion, enrichment, LC/MS/MS and analysis
  • Post-translational Modifications
    • Includes digestion, LC/MS/MS and analysis
  • Global Phosphoproteome Analysis
    • Includes digestion, enrichment, LC/MS/MS and analysis
  • Qualitative Phosphoproteomic Analysis
    • Includes digestion, enrichment, LC/MS/MS and analysis
  • Post-translational Modifications
    • Includes digestion, LC/MS/MS and analysis
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Phosphoproteomics

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Post-Translational Modification Analysis

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Includes digestion, LC/MS/MS and analysis

Includes digestion, LC/MS/MS and analysis

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Quantitative Proteomics

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  • Label Free
  • iTRAQ
  • SILAC
  • Heavy Peptide
  • Multiple Reaction Monitoring (MRM)
  • Label Free
  • iTRAQ
  • SILAC
  • Heavy Peptide
  • Multiple Reaction Monitoring (MRM)
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ELISA

Enzyme-linked immunosorbent assay
Price on request

Includes plate, analysis and report generation

Includes plate, analysis and report generation

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Glycosylation Analysis

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Gel Electrophoresis

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Up to 9 samples per gel

Up to 9 samples per gel

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Proteomics

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Antibody Services

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Spectroscopy

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Imaging & Spectroscopy

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Protein Services

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Liquid Chromatography Coupled Mass Spectrometry Methods

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Computational Systems Biology

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Mass Spectrometry

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Systems Biology

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Biochemistry & Molecular Biology

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Bioconjugation/Chemical Crosslinking

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Proteomics Data Analysis

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LC-MS

Liquid Chromatography-Coupled Mass Spectrometry
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In vivo Protein Binding Assays

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Analytical Chemistry Services

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Electrophoresis

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Custom Proteomics

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Structural Biology

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Computational Modeling

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Protein Sequencing

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Omics

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Biology

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Biomolecular Interaction Analysis

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Data Services

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John Schenkel

President & CEO

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