8van7tybq0idqes9u47z nehirarma üst eng

Nehir Biyoteknoloji

Ankara, TR

Nehir Biyoteknoloji has not listed any services.

Ask A Question Find what you're looking for? If not, you can ask this lab a question:
Single Nucleotide Polymorphism (SNP) Genotyping
Price on request

WE PROVIDE FREE CONSULTANCY FOR PROJECT PROPOSAL APPLICATION, IF YOU GET ONE OF OUR RESEARCH SERVICE IN YOUR PROJECT.

  1. Sanger Sequencing Analysis:

This is as well-known gold standard method for identification of any mutation or polymorphism in target genomic sequence. Process includes the fluorescent labelled ddNTP... Show more »

WE PROVIDE FREE CONSULTANCY FOR PROJECT PROPOSAL APPLICATION, IF YOU GET ONE OF OUR RESEARCH SERVICE IN YOUR PROJECT.

  1. Sanger Sequencing Analysis:

This is as well-known gold standard method for identification of any mutation or polymorphism in target genomic sequence. Process includes the fluorescent labelled ddNTP incorporation during singe strand synthesis and the products are read by using capillary electrophoresis device. The most efficient and maximum length is accepted to be 600 bases read.

You may prefer Sanger sequencing if your application is among the subjects below :

- Sequence analysis of unknown target genomic regions, De-novo sequencing, varification of NGS data, mitochondrial dna sequencing etc.
- HLA typing,
- Identification of unknown genomic variations related to a disease or protein aberrations
- Forensics
- Agriculture and Animal Breeding
- Identification of epigenetics related regions on DNA and methylation analysis
- Identification of microorganisms related pathogenic diseases or environmental conditions by 16S rRNA gene sequence analysis and metagenomics

  1. Restriction Endonuclease Analysis (REA)

Type-2 Restrion Endonuclease enzymes bind on and cut double-stranded DNA sequences with a sequence specific manner. They first discovered as an enzyme of bacterial defence mechanism against phages and then widely accepted in genome engineering. Restriction enzymes are both used in gene cloning and SNP genotyping applications.

Sanger sequencing is required for finding unknown genomic variations at specific locations, however it is expensive and time-consuming for scanning of large number of samples for a specific point. In this case, after amplification of target sequence by PCR, variations can be identified by observation of PCR products as cut or un-cut by specific restriction enzymes.

  1. Allele Specific PCR Analysis

Scanning of specific variations on samples can not be performed by Restriction Enzyme Assay because there is not enough identified type-2 endonucleases for all sequence possibilities. Additionally, same enzyme binding sequences may be present at very close to target variation. In this case REA wont work.

Fast SNP genotyping can also be performed by an alternative method called Allele-Specific PCR. This method uses specific primers of which 3'-end hybridizes on the polymorphic/mutant nucleotide. By making two PCR reactions with 2 forward primers and one cummon reverse primer, one can find the mutation by observing the PCR product lines on gel.

« Show less
Microfluidic Device Fabrication
Price on request

We aim to make the microfluidics technology more accessible and widespread among the various different disciplines. Inside the microchannels, flow dynamics is determined according to Reynault's number and when it is lower than 1, laminar flow is observed. Two different liquids flowing through the microchannel, follow a... Show more »

We aim to make the microfluidics technology more accessible and widespread among the various different disciplines. Inside the microchannels, flow dynamics is determined according to Reynault's number and when it is lower than 1, laminar flow is observed. Two different liquids flowing through the microchannel, follow a laminar flow and do not mix. Microfluidics chips are able to provide an easier way and more efficient data for your experiments of any subjects such as biology, chemistry, genetics, or nanotechnology.

Finding the best strategy and most suitable step to evaluate and ease your experimental work.
Design and simulation of most suitable microfluidics chip design made according to rules from scientific literature and needs of your research and experimental strategy.
Consultancy for the fabrication of your custom design microfluidic chips.

We prefer PDMS, PMMA and Glass as microchannel material.

PDMS microchannels are fabricated by soft lithography from SU-8 molds on Silicon wafers. After spincoating most suitable SU-8 material on Silicon wafers at desired thickness, microstructures are formed by maskless UV lithography. PMMA chips be ready by bonding on glass slides by oxygen plasma treatment.
PMMA microchannels are fabricated also by SU-8 molds on silicon wafers. The monomer of PMMA is applied on SU-8 mold and polimerized by a special technique. PMMA chips be ready by bonding on another PMMA sheet.
Glass microchannel fabrication are performed by isotropic wet etching of chrome and photoresist coated glass wafers. Using maskless UV lithography, features are copied on glass wafers and channels are formed chemically in glass. Chips be ready by bonding on another glass layer thermally at high temperatures.

We use our own designed and fabricated microfluidic fittings and connectors. Please take time to look at our "3D Printing" service in Science-Exchange.

« Show less
3D Printing
Three dimensional printing
Price on request

As the years advance with microfluidics based research projects, we have got experience on connectors and fittings. Many of the applications need tubings for fluidic inlets and syringes for pumping. Below please find our own designs of 3D-printed microfluidic fitting part for various different purposes.

Will be happy to hear... Show more »

As the years advance with microfluidics based research projects, we have got experience on connectors and fittings. Many of the applications need tubings for fluidic inlets and syringes for pumping. Below please find our own designs of 3D-printed microfluidic fitting part for various different purposes.

Will be happy to hear about your needs and can make CAD designs followed by fast 3D printing.

We can also provide the fitting parts in sterilized and packaged. Please also find the user manual of the fitting parts in our company website www.nehirbt.com.tr

« Show less
Laser Lithography
Price on request

Lithography is the term for microstructure formation on chips. Currently most of the microfluidic chips (except 3D printing chips) are fabricated by using UV, focused ion beam, electron beam, or laser beam to form desired shapes on wafers coated by photoresists or similar materials.

We currently use maskless laser lithography... Show more »

Lithography is the term for microstructure formation on chips. Currently most of the microfluidic chips (except 3D printing chips) are fabricated by using UV, focused ion beam, electron beam, or laser beam to form desired shapes on wafers coated by photoresists or similar materials.

We currently use maskless laser lithography system. The device uses a 405nm fiber laser with 1um condensed beam size and apply structures drawn by CAD softwares direcly on photoresists.

Important points during experimental protocol are as below:

The min feature size can be 1um (which is laser beam size) but this is also depends on the photoresist coating thickness. Photoresists thickness higher than 1um causes the higher min. feature sizes.
The application time of laser lithograpy depends on the total size of the CAD design. For longer process times, user should be sure about the endurance of exposed photoresist.

« Show less
Surface Plasmon Resonance (SPR)
Price on request

The theory is based on a physical process of light-metal film interaction. In SPR device, a material that has a high refractive index (with respect to medium) is coated with thin layer gold. When this gold coated material is treated with monochromatic light by a certain angle, some of the energy of light is transferred to free... Show more »

The theory is based on a physical process of light-metal film interaction. In SPR device, a material that has a high refractive index (with respect to medium) is coated with thin layer gold. When this gold coated material is treated with monochromatic light by a certain angle, some of the energy of light is transferred to free electrons of the gold and surface plasmons are formed on the gold. This plasmon will end up with specific refractive index. This phenomenon has been used for any molecular detection.

The application of SPR: On thin film gold surface, the molecule of interest is immobilized. This can be antibody, aptamer, cell, enzyme or etc. When the target molecule is sent to the media on top of molecule of interest and when association or dissociation happens between the immobilized olecule and target analyte, the refractive index will be changed which we can measured with high precision in real time by embedded sensors.

SPR technique has many advantages and label free detection is the most important advantage of it. SPR can be used to analyze biological or chemical materials and their molecular interactions without labeling molecules.

Real-Time analysis can be achieved with SPR techniques. Avidity and affinity of the samples can be detected. SPR technique offers almost at same level sensivity with ELISA and sometimes better.

Quantitave Analysis is an important feature with SPR devices. Every biological and chemical interaction can be monitored in details.

« Show less
Immunofluorescence
Price on request

Please get in contact if you are interested about this method.
Aptamer-fluorescence service is also available.
More information will be available soon here for both technique.

Please get in contact if you are interested about this method.
Aptamer-fluorescence service is also available.
More information will be available soon here for both technique.

« Show less
Aptamer Selection Service
Price on request

SELEX
Please get in contact if you are interested about this method. More information will be available soon here.

SELEX
Please get in contact if you are interested about this method. More information will be available soon here.

« Show less
Epigenomics Sequencing
Price on request

Please get in contact if you are interested about this method. More information will be available soon here.

Please get in contact if you are interested about this method. More information will be available soon here.

« Show less
Genotyping Services
Price on request
Request a quote for more information about this service.
Protein Expression Visualization
Price on request
Request a quote for more information about this service.
Epigenetic Analysis
Price on request
Request a quote for more information about this service.
Lithography
Price on request
Request a quote for more information about this service.
Physical Processing
Price on request
Request a quote for more information about this service.
Protein Services
Price on request
Request a quote for more information about this service.
Biology
Price on request
Request a quote for more information about this service.
Immunostaining
Price on request
Request a quote for more information about this service.
Aptamers
Price on request
Request a quote for more information about this service.
DNA Synthesis and Probe Development
Price on request
Request a quote for more information about this service.
Biomolecular Interaction Analysis
Price on request
Request a quote for more information about this service.
Microfabrication Services
Price on request
Request a quote for more information about this service.
Fabrication Services
Price on request
Request a quote for more information about this service.
Engineering
Price on request
Request a quote for more information about this service.
DNA
Price on request
Request a quote for more information about this service.
Biochemistry & Molecular Biology
Price on request
Request a quote for more information about this service.
Nucleic Acid Services
Price on request
Request a quote for more information about this service.

Nehir Biyoteknoloji has not received any ratings.

Be the first to endorse Nehir Biyoteknoloji

Endorse this lab