B8vkeesjtmsrhkoswpqi ntc

Nature Technology Corporation

Lincoln, NE, US

Nature Technology Corporation is a biotechnology company that provides industry partners with world class technology platforms including safe and efficacious non-viral vectors (plasmids) and processes for gene therapy and DNA vaccination vectore production.

NTC also provides cost effective outsourcing of product development services including synthetic gene design, vector construction, plasmid DNA manufacturing and recombinant proteins.

Recent Publications
- Plasmid Fermentation Process for DNA Immunization Applications. Carnes AE, Williams JA, Process for DNA Immunization Applications, in: Monica Rinaldi et al. (eds.), DNA Vaccines: Methods and Protocols, Methods in Molecular Biology, vol. 1143, p197-217, Springer Science+Business Media New York.
- Development of Antibiotic-free Selection System for Safer DNA Vaccination. Luke JM, Carnes AE, Williams JA, Development of Antibiotic-Free Selection System... Show more »

Nature Technology Corporation is a biotechnology company that provides industry partners with world class technology platforms including safe and efficacious non-viral vectors (plasmids) and processes for gene therapy and DNA vaccination vectore production.

NTC also provides cost effective outsourcing of product development services including synthetic gene design, vector construction, plasmid DNA manufacturing and recombinant proteins.

Recent Publications
- Plasmid Fermentation Process for DNA Immunization Applications. Carnes AE, Williams JA, Process for DNA Immunization Applications, in: Monica Rinaldi et al. (eds.), DNA Vaccines: Methods and Protocols, Methods in Molecular Biology, vol. 1143, p197-217, Springer Science+Business Media New York.
- Development of Antibiotic-free Selection System for Safer DNA Vaccination. Luke JM, Carnes AE, Williams JA, Development of Antibiotic-Free Selection System for Safer DNA Vaccination, in: Monica Rinaldi et al. (eds.), DNA Vaccines: Methods and Protocols, Methods in Molecular Biology, vol. 1143, p 91-111, Springer Science+Business Media New York.
- Improving DNA Vaccine Performance Through Vector Design. Williams, J.A. (2014) Current Gene Ther. 14:170-189.
- Vector optimization and needle-free intradermal application of a broadly protective polyvalent influenza A DNA vaccine for pigs and humans. Borggrena M, Nielsena J, Bragstada K, Karlssona I, Jesper S. Krog JS, Williams JA, Fomsgaardad A DOI:10.1080/21645515.2015.1011987
- Strengthening the Skin with Topical Delivery of Keratinocyte Growth Factor-1 using a Novel DNA Plasmid. Dou C1, Lay F2, Ansari AM2, Rees DJ2, Ahmed AK2, Kovbasnjuk O3, Matsangos AE2, Du J2, Hosseini SM2, Steenbergen C4, Fox-Talbot K4, Tabor AT5, Williams JA6, Liu L2, Marti GP2, Harmon JW2., Mol Ther. 2014 Apr;22(4):752-61. doi: 10.1038/mt.2014.2. Epub 2014 Jan 17.

« Show less

Nature Technology Corporation has not listed any services.

Plasmid DNA Preparation
Price on request

Advantages of NTC’s Plasmid Manufacturing Capabilities

  • Highest yields ( > 2.6 g/L)
  • Improved % supercoiled monomer using reduced metabolic burden process
  • Highest quality purification (>95% supercoiled plasmid DNA)
  • Highest plasmid yields per unit biomass
  • Lowest cost per unit DNA
  • Vector to process matching... Show more »

Advantages of NTC’s Plasmid Manufacturing Capabilities

  • Highest yields ( > 2.6 g/L)
  • Improved % supercoiled monomer using reduced metabolic burden process
  • Highest quality purification (>95% supercoiled plasmid DNA)
  • Highest plasmid yields per unit biomass
  • Lowest cost per unit DNA
  • Vector to process matching for highest overall performance
  • Improved yield of toxic or unstable plasmids
  • Save 20% of large scale production cost with NTC’s high-yield vectors

NTC’s In-house pDNA services

  • Small- and large-scale production and purification of research-grade pDNA (1mg-multi-gram)
  • Quality testing of pDNA products (endotoxin [LAL]; residual RNA [gel], DNA [qPCR], protein [modified BCA testing])
  • Production of plasmid-enriched E. coli cell paste for downstream processing in client facilities
  • E. coli strain engineering, for production hosting of specific toxic plasmids
  • Plasmid backbone engineering for substitution of antibiotic-free (markerless) or high copy plasmid backbones
  • Low metabolic burden technology for stable production of toxic or unstable plasmids
  • Production of Research Cell Banks (RCB) using low metabolic burden technology
  • Transfer of RCB to GMP facility for certification as Master and Working Cell Banks
  • Technology Transfer of HyperGRO™ process to CMOs and end-user facilities for best-in-class performance
« Show less
Large Scale Plasmid Prep
Price on request

Large Scale Plasmid DNA Purification and Production (100mg-multi gram) Low Endotoxin Plasmid DNA Purification and Processing

Large scale DNA plasmids at NTC are grown in a fed-batch fermentation process, permitting control over feed rate, pH, temperature, and dissolved oxygen. NTC’s patented HyperGRO™ fermentation process and... Show more »

Large Scale Plasmid DNA Purification and Production (100mg-multi gram) Low Endotoxin Plasmid DNA Purification and Processing

Large scale DNA plasmids at NTC are grown in a fed-batch fermentation process, permitting control over feed rate, pH, temperature, and dissolved oxygen. NTC’s patented HyperGRO™ fermentation process and media has produced record pDNA yields of >2.6g/L, and typically produce 0.5-1.5g/L using a high copy pUC, or similar origin, and a recA-, relA-, endA- host strain, such as DH5 alpha. Highly concentrated HyperGRO™ plasmid preparations naturally result in cleaner, more highly-purified DNA (due to dramatically reduced biomass), and more cost-effective production as well. (See related molecular services below)

A large-scale certificate of analysis will be provided including gel photo (digested and undigested DNA, ladder), concentration and quantity:

Large Scale Certificate of Analysis, (pdf) including:

  • Spectrophotometric analysis (A260/280)
  • Quantity
  • Concentration (usually 0.5mg/ml, in TE buffer)
  • Volume
  • Gel photo
  • Residual RNA (gel assay)
  • Residual genomic E. coli DNA (qPCR)
« Show less
Endotoxin Testing
Price on request
Request a quote for more information about this service.
qPCR
Quantitative PCR
Price on request
Request a quote for more information about this service.
DNA Vaccine and Gene Therapy Vectors
Price on request

Precision Cloning is a streamlined process whereby NTC provides you with custom-finished DNA vaccine vectors, sequence-validated and ready for animal testing, in record time - a new weapon in the arsenal against infectious diseases, bioterrorism and cancer therapy.

Vector Design:

NTC scientists work with clients for design... Show more »

Precision Cloning is a streamlined process whereby NTC provides you with custom-finished DNA vaccine vectors, sequence-validated and ready for animal testing, in record time - a new weapon in the arsenal against infectious diseases, bioterrorism and cancer therapy.

Vector Design:

NTC scientists work with clients for design optimization

Insert Choices:

  • Client Genes
  • External Sources
  • Optimized Synthetic Genes

Vector Choices:

  • Optimized NTC DNA Vaccine Vectors
  • Optimized NTC Therapeutic Gene Vectors

Technology Advantages:

  • Vectors optimized for: speed (4-5 weeks typical turnaround times); economy ($400 typical cost per antigen, includes sequencing inserts), regulatory compliance (Points to Consider); optimal prokaryotic plasmid yield; optimal mammalian transient expression; choice of markerless (antibiotic-free) or KanR; immunogenicity.
  • Technology Options: targeting, dcm methyla-ion (+/-), RIG-1 activation (interferon)

Clone Validation:

Fully sequenced inserts and junctions

Plasmid DNA Manufacturing:

Highly purified, endotoxin-free, research grade pDNA

  • Small Scale: 1-99mg
  • Large Scale: 100mg-Kg
  • (HyperGRO™) Fermentation, highest attainable yields and purity)
« Show less
Plasmid Detox Services
Price on request

An NTC strain engineering service for improving yields of toxic plasmids.

Plasmid Detox Advantages:

  • Easy – NTC’s Detox shuttle makes it fast and simple to knock down expression of toxic genes in E. coli during plasmid DNA production
  • Economical - $1,249 includes: vector; cloning; strain engineering; and testing
    -... Show more »

An NTC strain engineering service for improving yields of toxic plasmids.

Plasmid Detox Advantages:

  • Easy – NTC’s Detox shuttle makes it fast and simple to knock down expression of toxic genes in E. coli during plasmid DNA production
  • Economical - $1,249 includes: vector; cloning; strain engineering; and testing
  • Effective – 10 fold, or more, improvement in plasmid yield is often possibleNTC’s shuttle for E. coli engineering

Plasmid Detox strain engineering for improved yields of toxic plasmids

Plasmid yields in fermentation culture typically range up to 2.6g/L, or more, depending on the type of plasmid, its origin of replication, the host strain, and whether or not it is toxic to the host cell. Some genes are toxic to E. coli, making it difficult to grow the quantities needed, and resulting in mutations and deletions.

NTC has devised a novel and economical strain engineering service, wherein the host strain chromosome of E. coli is modified to include a gene that is expressed as antisense to the plasmid-encoded toxic gene. Elimination of the toxic gene products allows the plasmid to be grown to the high levels needed for producing larger quantities of plasmid.

« Show less
Biology
Price on request
Request a quote for more information about this service.
Cells and Tissues
Price on request
Request a quote for more information about this service.
Vaccines
Price on request
Request a quote for more information about this service.
Testing of Cell Lines
Price on request
Request a quote for more information about this service.
PCR
Price on request
Request a quote for more information about this service.
DNA
Price on request
Request a quote for more information about this service.
Plasmids and Vectors
Price on request
Request a quote for more information about this service.
Drug Discovery & Development
Price on request
Request a quote for more information about this service.
Biochemistry & Molecular Biology
Price on request
Request a quote for more information about this service.
Drug Discovery
Price on request
Request a quote for more information about this service.
Nucleic Acid Services
Price on request
Request a quote for more information about this service.
Ask A Question Find what you're looking for? If not, you can ask this lab a question:

Nature Technology Corporation has not received any ratings.

Be the first to endorse Nature Technology Corporation

Endorse this lab