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NANOBIOTEC, LLC

ic 7.3/10 Overall Rating 83% Positive
ic 24 Orders Completed
Hanover, New Jersey, US

About NANOBIOTEC, LLC

Founded: 2012 Type: Privately Held Size: 1-10 employees

NANOBIOTEC® provides most sensitive and reliable bioassay services to monitor real-time protein and gene changes in minimal samples from cell culture, animals and patients to accelerate discovery and development of new therapeutics. We provide cost-effective services to the biopharma industry and academic... Show more »

NANOBIOTEC® provides most sensitive and reliable bioassay services to monitor real-time protein and gene changes in minimal samples from cell culture, animals and patients to accelerate discovery and development of new therapeutics. We provide cost-effective services to the biopharma industry and academic investigators to accelerate discovery and development of novel drugs and personalized medicine for cancer, inflammation, and many other unmet diseases.

Our Platforms

NANOBIOTEC® has established a unique infrastructure with cutting-edge technologies and state-of-the-art instruments:
1. Simple Western Assays (automation of western blot; Capillary-based Nano-Proteomic Immunoassay);
2. Luminex-based Multiplex Immunoassay;
3. Flow cytometry (CytoFlex S with 4 lasers and 13 colors)
4. Simoa-based Ultrasensitive Immunoassay;
5. Protein arrays (such as RPPA)
6. 7900HT Fast Real-Time PCR System
7. IncuCyte Zoom live cell imaging
8. LiCor Odyssey Near-Infrared Imaging System
9. LC-MS/MS for Protein Identification, Proteome Profiling & Characterization
10. ELISA / ELISpot
11. Western Blot
12. Invitrogen EVOS FL Auto Cell Imaging System

Publications

  1. Fan AC, Deb-Basu D, Orban MW, et al. Nanofluidic proteomic assay for serial analysis of oncoprotein activation in clinical specimens. Nat Med. 2009; 15(5):566-71.
  2. Nishizuka S, Charboneau L, Young L, et al.. Proteomic profiling of the NCI-60 cancer cell lines using new high-density reverse-phase lysate microarrays. Proc Natl Acad Sci U S A. 2003;100(24):14229-34.
  3. Schafer, P.H., Truzzib, F. Parton, A., Wu, L., Kosek, J., Zhang, L.H., et al., "Phosphodiesterase 4 in inflammatory diseases: effects of apremilast in psoriatic blood and in dermal myofibroblasts through the PDE4/CD271 complex", Cellular Signalling. Vol. 28, pp 53-63, 2016
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Diversity Certificates

Small business

Our Services (192)


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Immunocytochemistry

Price on request
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Immuno-oncology Assays

Price on request
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Immunofluorescence

Price on request
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Live Cell Imaging

Price on request
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Luminex Multiplex Assays

Price on request

We simultaneously examine multiple analytes in a single sample (as little as 10 uL per sample from human, mouse or rats), such as over 60 cytokines/chemokines, and many proteins (expression or phosphorylation) involved in signaling pathways of cancer, apoptosis, acute phase response, inflammation, immunity, diabetes, kidney... Show more »

We simultaneously examine multiple analytes in a single sample (as little as 10 uL per sample from human, mouse or rats), such as over 60 cytokines/chemokines, and many proteins (expression or phosphorylation) involved in signaling pathways of cancer, apoptosis, acute phase response, inflammation, immunity, diabetes, kidney toxicity, etc. We also simultaneously interrogate multiple histone H3 PTMs, transcription factors-specific DNA binding activity, and expression of genes.

Capabilities:
• Kit-based (we can buy or can be provided by the researcher), custom designed Luminex kits
• we can do extraction / homogenization/ Sonication of cell/tissue lysates, if needed
• we do optimization of protocols for commercial kits, if needed
• we analyze serum, plasma, cell lysates, cell culture supernatants
• we measure cytokines /transcription factors /protein phosphorylation/histone acetylation/ mRNA, etc
• we can measure protein and mRNA in a single sample simultaneously
• Number of samples processed in a single experiment: duplicate or triplicate, 96-well plate format

Equipment:
• Bio-Plex Pro wash stations
• BioRad Bio-Plex 200

Sample requirements:
• All samples should be Bio-Safety Level II or below.
• All samples should be submitted in ready-to-assay form.
• Any extraction or preparation procedures are the responsibility of the researchers, unless arrangements have been made in advance with us.
• Samples should be submitted in a minimum-binding 96-well plate following a standard assay design layout and accompanied by a template that outlines the sample order.
• Please provide sufficient volume for all samples to be assayed, 50-200 ul depending on duplicate or triplicate.
• Please ship samples in dry ice.

Turnaround time:
1-2 weeks or sooner, upon receiving test samples/reagent kits

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Luminex®

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Flow Cytometry

Price on request

• Intracellular Cytokine Secretion (ICS) – Identification and enumeration of cells that are secreting factors (cytokines)
• Cell cycle analysis – Identification of cellular division stages.
• Cell proliferation – Identification and enumeration of cellular proliferation levels.
• Cell viability and apoptosis – Identification and... Show more »

• Intracellular Cytokine Secretion (ICS) – Identification and enumeration of cells that are secreting factors (cytokines)
• Cell cycle analysis – Identification of cellular division stages.
• Cell proliferation – Identification and enumeration of cellular proliferation levels.
• Cell viability and apoptosis – Identification and numeration of dead (necrotic) and dying (apoptotic) cells.
• Cell phenotyping – Identification and enumeration of different types of cells in heterogeneous mixture.
• Phosphoprotein analysis
• DNA and RNA analysis
• Translocation assays
• antibody screening
• rare event analysis, such as in dendritic cells, and stem/progenitor cells
• Cell line characterization for any markers interested
• Cell sorting
• calcium flux analysis
• disease-associated biomarker analysis and monitoring
• Multiplexed Bead-Based Immunoassays (such as cytokines, microRNAs)

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Immunology

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T Cell Activation and Proliferation Assays

Price on request
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Mixed Lymphocyte Reactions

Price on request

A common method to assess the cellular immune response is to co-culture lymphocyte populations from two individuals, (e.g. two humans or two mice of different strains) where the T cells recognize the foreign cells from the other strain, activate and proliferate. One population of cells can be restricted to being stimulator cells... Show more »

A common method to assess the cellular immune response is to co-culture lymphocyte populations from two individuals, (e.g. two humans or two mice of different strains) where the T cells recognize the foreign cells from the other strain, activate and proliferate. One population of cells can be restricted to being stimulator cells (e.g. a preparation of tumor cells), by pretreating them with a mitotic poison (mytomycin B), thereby turning the assay into "one-way" MLR.

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ADCC and CDC Assays

Price on request

Antibody-dependent cell-mediated cytotoxicity assay (ADCC)
We provide ADCC assay service to facilitate the selection of antibody drug candidates and compound candidates.

Capabilities:

• We can use PBMC or/and natural killer cells as the effector cells.
• We provide a series of target cells for testing including but not... Show more »

Antibody-dependent cell-mediated cytotoxicity assay (ADCC)
We provide ADCC assay service to facilitate the selection of antibody drug candidates and compound candidates.

Capabilities:

• We can use PBMC or/and natural killer cells as the effector cells.
• We provide a series of target cells for testing including but not limited to

CD20+ cells: Raji, Daudi, Ramos;
Her2+ cells: SK-BR-3, SK-OV-3, MCF-7;
EGFR+ cells: PC-3, A549, HaCaT

• Appropriate antibodies such as rituximab (anti-CD20), trastuzumab (anti-Her2) or cetuximab (anti-EGFR) are included as positive controls.
• You can also submit your target cell lines. We will culture them and test your samples for ADCC activity.
• Cell lysis is accurately quantified based on standard LDH release assay or/and calcein AM assay.
• We can optimize your antibody concentrations and effector/target ratios for testing.
• Assays are performed using a 96-well format. Each sample is tested in duplicate or triplicate.

Equipment:

• Multi-channel pipets
• Automatic pipets
• Tecan florescence and absorbance plate reader

Sample requirements:

• All samples should be Bio-Safety II or below.
• All cell lines sent to us must be shipped frozen on dry ice in cryopreservation vials or as growing cultures in flasks at ambient temperature.
• Please clearly label samples and provide sufficient volume for all samples.
• Small molecule compounds can be provided as powders or prepared as 1000x stock solutions in DMSO and shipped frozen on dry ice.
• Antibodies must be prepared in PBS at 100x – 1000x top concentration and shipped on dry ice.
• Submit a standard assay design layout and a template that outlines the sample order along with your samples.

Typical turn-around time:

• 2-3 weeks or sooner, upon receiving all materials.

CDC Assays
Target cells are suspended in media alone, media with 30-1 % serum from blood samples, or media with heat-inactivated serum. Cells are then treated with 10000-10 ng/ml test antibody at 37C for 1 h
Standard lactate dehydrogenase (LDH) release assay (CytoTox 96 Nonradioactive Cytotoxicity Assay; Promega) to measure CDC.

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Real-Time Cytotoxicity Assay

Price on request

We offer real-time cytotoxicity assays using our IncuCyte Live Cell Analysis System or the Invitrogen EVOS FL Auto Imaging System.

We offer real-time cytotoxicity assays using our IncuCyte Live Cell Analysis System or the Invitrogen EVOS FL Auto Imaging System.

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BioTek Cytation 5

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ADCP Assay

Antibody-Dependent Cell-Mediated Phagocytosis Assay
Price on request

Antibody-Dependent Cell-Mediated Phagocytosis Assay

Antibody-Dependent Cell-Mediated Phagocytosis Assay

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Cytokine Analysis

Price on request

The biological activity of cytokines is measured by cellular proliferation of primary cell cultures
or in vitro adapted cell lines that are dependent and/or responsive to a specific growth factor.

Other aspects of biological activity of cytokines include induction of further cytokine secretion, induction of
killing,... Show more »

The biological activity of cytokines is measured by cellular proliferation of primary cell cultures
or in vitro adapted cell lines that are dependent and/or responsive to a specific growth factor.

Other aspects of biological activity of cytokines include induction of further cytokine secretion, induction of
killing, antiviral activity, degranulation, cytotoxicity, chemotaxis, and promotion of colony formation.

In vitro assays to measure all of these activities are provided.

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Mitochondrial Stress Tests

Price on request
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Cytokine Storm Testing

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Clinical Biomarkers

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Ligand Binding Assays

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In-Cell Western Assay

Price on request

Our assay combines the specificity of Western blot with the reproducibility and throughput of ELISA, is a simple and cost-effective means for quantification of intracellular signaling in whole cells.

This assay involves seeding cells in 96- or 384-well microtiter plates followed by fixation/permeabilization and subsequent... Show more »

Our assay combines the specificity of Western blot with the reproducibility and throughput of ELISA, is a simple and cost-effective means for quantification of intracellular signaling in whole cells.

This assay involves seeding cells in 96- or 384-well microtiter plates followed by fixation/permeabilization and subsequent labeling with activation state-specific or control antibodies, infrared-conjugated secondary antibodies, and/or far-red DNA dye.

The well-level data acquired by the Odyssey® Imaging System permits accurate measurement of signal induction or inhibition, with the potential for multiple treatments, end points, and replicates on each plate.

Our assays quickly, accurately measure relative protein levels in many samples, detect proteins in situ in a relevant cellular context.

This is used for high-through put analysis of: protein phosphorylation (effects of drug compounds on signaling pathways; timing/kinetics of signal transduction), caspase-3 activation, cell surface proteins and receptor internalization, RNAi library screens, cell proliferation, etc.

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On-Cell Western Assay

Price on request

Fixed but unpermeabilized cells are stained with antibodies against extracellular protein domains, so only cell surface antigens are detected. Cell surface receptors are detected with Odyssey Infrared Imaging System.

Our assay can quantify target proteins localized to the cell surface, enabling quantitative monitoring of cell... Show more »

Fixed but unpermeabilized cells are stained with antibodies against extracellular protein domains, so only cell surface antigens are detected. Cell surface receptors are detected with Odyssey Infrared Imaging System.

Our assay can quantify target proteins localized to the cell surface, enabling quantitative monitoring of cell surface protein expression.

This assay can be a high throughput assay without using radioactive labeling, to quantify ligand binding to cell surface receptors, monitor receptor internalization, and evaluate of drug effects on protein trafficking, etc.

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In vitro Immunogenicity Assays

Price on request
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Luminex microRNA (miRNA) Microarray

Price on request
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Western Blot

Price on request

Simple Western - Nanoproteomic Immunoassay – Automation of Western Blot

We offer both traditional western blot and Simple Western (capillary based nanoproteomic immunoassay –- High through-put Western Blot)

We can quantify both absolute and relative amounts of any protein (even rare or low abundance) in samples of limit... Show more »

Simple Western - Nanoproteomic Immunoassay – Automation of Western Blot

We offer both traditional western blot and Simple Western (capillary based nanoproteomic immunoassay –- High through-put Western Blot)

We can quantify both absolute and relative amounts of any protein (even rare or low abundance) in samples of limit amount (as low as 25 cells),

Samples can be those from cells of live animals and patients including fine needle aspirates (FNA), laser capture microdissection (LCM), FFPE samples, sorted cells, as well as serum, plasma, saliva, urine, etc.

Capabilities:
• Most sensitive immunoassay to test proteins in a minimal amount sample;
• quantify protein phosphorylation even without phospho-specific antibodies;
• We can separate proteins based on their sizes (MW) or electric charges (pI values)
• Over 100 cancer cell lines and primary cells available for testing drugs based on clients’ requirements
• we can do homogenization, and preparation of tissue lysates
• we can do protein concentration/ normalization using BCA assay
• Commonly used antibodies available
• Optimize protocols for particular antigen/target

Equipment:
• ProteinSimple Wes
• ProteinSimple Peggy
• Novex® SDS-PAGE gels
• BioRad equipment

Sample requirements:
• All samples should be Bio-Safety Level II or below.
• All samples (1 or 2 mg/ml protein) should be submitted in ready-to-assay form.
• Any extraction or preparation procedures are the responsibility of the researchers, unless arrangements have been made in advance with us.
• Samples should be submitted in a minimum-binding microfuge tubes and accompanied by a template that outlines the sample order.
• Please provide sufficient volume for all samples to be assayed
• Please ship samples in dry ice.
Turnaround time:
• 1 day -2 weeks, upon receiving test samples/ primary antibodies

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Protein Simple WES

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Cell Signaling Analyses

Price on request
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Immune Checkpoint Assays

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Immunophenotyping

Price on request
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Cell Viability Assays

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Cell Death Assays

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ALPHAlisa

Price on request
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Phagocytosis Assay

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ELISpot Assays

Enzyme-Linked ImmunoSpot Assays
Price on request
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Antibody Characterization

Price on request
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Multiplex Gene Expression Analysis

Price on request

Multiplex nucleic acid testing allows multiplexed gene expression analysis (RNA targets) and DNA copy number variation (CNV) analysis, to measure up to 80 genes in a single sample. mRNA data is directly from cell/tissue lysates to avoid biases, sample loss, false results.

Multiplex nucleic acid testing allows multiplexed gene expression analysis (RNA targets) and DNA copy number variation (CNV) analysis, to measure up to 80 genes in a single sample. mRNA data is directly from cell/tissue lysates to avoid biases, sample loss, false results.

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Reverse Phase Protein Array (RPPA)

Starting at $200.00 per sample

A high-throughput antibody-based microarray with the procedures similar to Western blots.
Proteins are extracted from tumor tissue or cells, denatured by SDS, printed on nitrocellulose- coated slides followed by antibody probe.

Capabilities:

• Currently allows for the analysis of >1000 samples using >300 different... Show more »

A high-throughput antibody-based microarray with the procedures similar to Western blots.
Proteins are extracted from tumor tissue or cells, denatured by SDS, printed on nitrocellulose- coated slides followed by antibody probe.

Capabilities:

• Currently allows for the analysis of >1000 samples using >300 different antibodies for human and mouse samples.
• Inexpensive, high-throughput method utilizing automation for increased quality and reliability
• Simple sample preparation, which is similar to Western blots
• Robust quantification due to serial dilution and replicates of samples
• characterization of cellular signaling networks under different conditions
• determination of drug selectivity and Identify therapeutic targets
• Defining regulatory mechanisms in signaling networks, including forward and feedback loops and cross talks
• Classifying patient tumors, determining prognosis and predicting responses to targeted therapies
• Understanding pharmacodynamics and biologically relevant dosing and schedule
• Determination of appropriate handling procedures for clinical samples (based on antigen stability analysis, etc.)

Equipment:

• Multi-channel pipets
• Automatic pipets
• Tecan Plate Reader
• Nano-Plotter NP2.1
• Zeptosens platform
• ZeptoREADER

Sample requirements:

• All samples should be Bio-Safety Level II or below.
• All samples (about 10-100 ul of 1 or 2 mg/ml protein) should be submitted in ready-to-assay form.
• Any extraction or preparation procedures are the responsibility of the researchers.
• Special arrangements can been made in advance with us.
• Samples should be submitted in a minimum-binding microfuge tubes and accompanied by a template that outlines the sample order.
• Please provide sufficient volume for all samples to be assayed
• Please ship samples in dry ice.

Turnaround time:

• 2 months or sooner, upon receiving test samples

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Fluorescence Polarization

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Fluorescence-Based Assays

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Cosmetic and Personal Care Product Testing

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Phosphoflow

Price on request
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Oxidative Stress Assays

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Human Epidermal Keratinocytes

Price on request
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Human Primary Cells

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DNA Damage & Repair Assays

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Cell Cycle Analysis

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Macrophage Activation Assay

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Degranulation Assay

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B Cell Activation Assay

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ELISA

Enzyme-linked immunosorbent assay
Price on request

Capabilities:
• Kit-based (we can buy or can be provided by the researcher), custom designed ELISA
• we can do extraction / homogenization/ Sonication of cell/tissue lysates, if needed
• we do optimization of protocols for commercial kits, if needed
• we analyze serum, plasma, cell lysates, cell culture supernatants
• we... Show more »

Capabilities:
• Kit-based (we can buy or can be provided by the researcher), custom designed ELISA
• we can do extraction / homogenization/ Sonication of cell/tissue lysates, if needed
• we do optimization of protocols for commercial kits, if needed
• we analyze serum, plasma, cell lysates, cell culture supernatants
• we measure cytokines /transcription factors /protein phosphorylation/histone acetylation, etc
• Number of samples processed in a single experiment: duplicate or triplicate, 96-well plate format

Equipment:

• Multi-channel pipets
• Automatic pipets
• Automatic Plate washers
• Tecan Plate Reader

Sample requirements:
• All samples should be Bio-Safety Level II or below.
• All samples should be submitted in ready-to-assay form.
• Any extraction or preparation procedures are the responsibility of the researchers, unless arrangements have been made in advance with us.
• Samples should be submitted in a minimum-binding 96-well plate following a standard assay design layout and accompanied by a template that outlines the sample order.
• Please provide sufficient volume for all samples to be assayed, 50-200 ul depending on duplicate or triplicate.
• Please ship samples in dry ice.

Typical turn-around time:
• 1-2 weeks or sooner, upon receiving all materials

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Cytotoxic T Cell Assays

Price on request
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Biomarker Discovery

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Autophagy Detection Assay

Price on request
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Cytometric Bead Array (CBA) FACS Analysis

Price on request
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Human Bone Marrow

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Mitochondrial Apoptosis Assay

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Transcriptomics Data Analysis

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MTT and MTS Assays

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Autoantibody Testing

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Acute Kidney Failure Models

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MicroRNA (miRNA) Microarray

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Quantitative Western Blot Analysis

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ROS Assays

Reactive Oxygen Species Assays
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Ultra-Sensitive Simoa Assays

Price on request
  • A fully automated, ultra-sensitive, multiplexed immunoassay or Digital ELISA.
  • Detection and quantification of low-abundance biomarkers difficult or impossible to measure with conventional assays.
  • Quantification of proteins as low as femtogram (fg)/ml in the samples
  • The digital nature of the Simoa technology allows an... Show more »
  • A fully automated, ultra-sensitive, multiplexed immunoassay or Digital ELISA.
  • Detection and quantification of low-abundance biomarkers difficult or impossible to measure with conventional assays.
  • Quantification of proteins as low as femtogram (fg)/ml in the samples
  • The digital nature of the Simoa technology allows an average of 1000 x sensitivity increase over conventional ELISA assays with CVs < 10%.
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Lipid Peroxidation Assay

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Phosphorylation Analysis

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Compound IC50 Assays

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Caspase Assays

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Histone Deacetylase (HDAC) Assays

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Histone Acetyltransferase (HAT) Assays

Histone Acetyltransferase Assays
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Paraffin Sectioning

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Biomarker Analysis

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In vitro Wound Healing Assay

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Quantitative Proteomics

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Biochemical Compound Screening

Price on request

Anti-Microtubule Compound Screening

Anti-Microtubule Compound Screening

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Microtubules

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Cancer Cell Line Panels

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Claim Substantiation

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Delayed-Type Hypersensitivity (DTH) Animal Models

Delayed Type Hypersensitivity Animal Models
Price on request
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Stem Cell Assays

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CTL Assay

Cytotoxic T Lymphocyte Assays, CTL and Patient-Matched Tumor Assays, Patient-Specific CTL Assays
Price on request
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Diabetes Animal Models

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Zebrafish Research Consultation

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3D Cell-Based Assays

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In vitro Psoriasis Models

Price on request
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Mast Cell Degranulation Test

Price on request
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Psoriasis Animal Models

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Rheumatoid Arthritis Animal Models

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Autoimmune Disease Animal Models

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Simple Western™

Price on request

We offer both traditional western blot and Simple Western (capillary based nanoproteomic immunoassay –- High through-put Western Blot)

We can quantify both absolute and relative amounts of any protein (even rare or low abundance) in samples of limit amount (as low as 25 cells),

Samples can be those from cells of live animals... Show more »

We offer both traditional western blot and Simple Western (capillary based nanoproteomic immunoassay –- High through-put Western Blot)

We can quantify both absolute and relative amounts of any protein (even rare or low abundance) in samples of limit amount (as low as 25 cells),

Samples can be those from cells of live animals and patients including fine needle aspirates (FNA), laser capture microdissection (LCM), FFPE samples, sorted cells, as well as serum, plasma, saliva, urine, etc.

Capabilities:
• Most sensitive immunoassay to test proteins in a minimal amount sample;
• quantify protein phosphorylation even without phospho-specific antibodies;
• We can separate proteins based on their sizes (MW) or electric charges (pI values)
• Over 100 cancer cell lines and primary cells available for testing drugs based on clients’ requirements
• we can do homogenization, and preparation of tissue lysates
• we can do protein concentration/ normalization using BCA assay
• Commonly used antibodies available
• Optimize protocols for particular antigen/target

Equipment:
• ProteinSimple Wes
• ProteinSimple Peggy
• Novex® SDS-PAGE gels
• BioRad equipment

Sample requirements:
• All samples should be Bio-Safety Level II or below.
• All samples (1 or 2 mg/ml protein) should be submitted in ready-to-assay form.
• Any extraction or preparation procedures are the responsibility of the researchers, unless arrangements have been made in advance with us.
• Samples should be submitted in a minimum-binding microfuge tubes and accompanied by a template that outlines the sample order.
• Please provide sufficient volume for all samples to be assayed
• Please ship samples in dry ice.
Turnaround time:
• 1 day -2 weeks, upon receiving test samples/ primary antibodies

Background:
Nanopro 1000 is a charge-based protein separation instrument using capillary nanoimmunoassay technology, and is used for characterizing and quantifying post-translational protein modifications in cell/ tissue/tumor lysates.

Service Details:
Many post-translational modifications result in changes in pI (isoelectric point). Nanopro 1000 can resolve a wide variety of charge variants in capillary and detect them by phospho-specific or pan-antibodies. It is therefore capable of detecting phosphorylated, acetylated, or methylated proteins. Meanwhile, this system is able to make rapid, quantitative analysis of specific proteins by using as few as 25 cells per assay.

Protein: The methods of phospho-protein detection have been validated by us, and over hundreds of other proteins can be detected upon request.
Plate formats: 96-well plate
Number of doses per compound: 9-10 dose IC50 format, or single dose
Number of replicates: Triplicates, duplicates, etc
Data analysis: % area or % phosphorylation analyzed by Compass Software

Deliverable: Gel picture and graph, analyzed data, QC results, an official final report

Sample Submission: Testing compounds can be submitted as dry powder or in DMSO stock,or cell lysate or tissue/tumor lysate in dry ice.

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ProteinSimple NanoPro 1000

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In vitro Immunotoxicity Testing

Price on request
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In vitro Angiogenesis Assays

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Human Immune Cell Assays

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Cell Migration Assays

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Cell Adhesion Assays

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Nanoproteomic Proximity Ligation Assay

Starting at $10.00 per sample per analyte

we simultaneously quantify 96 proteins in 96 samples in one experiment with our optimized primer-antibody pairs and PCR, currently focusing on cancer biomarkers.



Turnaround time: 1-2 weeks upon receiving test samples/reagent kits

we simultaneously quantify 96 proteins in 96 samples in one experiment with our optimized primer-antibody pairs and PCR, currently focusing on cancer biomarkers.



Turnaround time: 1-2 weeks upon receiving test samples/reagent kits

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High Content Screening

Price on request
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Statistical Analysis

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Phosphoproteomics

Price on request
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Immunoprecipitation

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Assay Development

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Gel Staining

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Cell Viability & Proliferation Assays

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Enzyme Assays

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Proteomics

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Metabolic Animal Models

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Nucleic Acid Services

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Clinical Chemistry

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Immunoassays

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Immunology Animal Models

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Cells and Tissues

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Drug Discovery & Development

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Antibody Services

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Bioanalysis

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Computational Modeling

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Biology

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Cytometry

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Cell-Based Assays

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Protein Quantification

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Human Biospecimens

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Clinical Immunology Tests

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Clinical Research

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Clinical Laboratory Services

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RNA Microarray

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Separation/Purification Services

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Nephrology Animal Models

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Spectroscopy

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Cellular Health & Metabolism Assays

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Biomarkers

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Hit Identification

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RNA Services

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Drug Discovery

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Biochemical Assays

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Analytical Chemistry Services

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Product Testing Services

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Animal Models of Disease

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Project Management

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Cell Invasion & Migration Assays

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Immune Cell Assays

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Cosmetic and Personal Care Product Formulation

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Dermatology Animal Models

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Omics

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Imaging & Spectroscopy

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Angiogenesis & Endothelial Cell Assays

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Toxicology

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Cell-Based Screening Methods

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ADME/DMPK Studies

Drug Metabolism and Pharmacokinetics
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Pharmacology & Toxicology

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Chemistry and Materials

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Cytotoxicity Assays

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Data Services

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Hydrolase Assays

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Antibody/Antigen Detection Based Testing

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Transferase Assays

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Protein Services

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Tissue Processing

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Protein Expression Visualization

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Specialized Cell-Based Assays

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In vitro Disease Models

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Protein-Protein Interaction Analysis

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Inflammation Animal Models

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Biomolecular Interaction Analysis

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Human Tissue

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In vitro Toxicity Testing

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Product Development, Testing, and Packaging

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Animal Models and Studies

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Fluorescence Spectroscopy

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Functional & Cell Type Specific Assays

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Biochemistry & Molecular Biology

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Biospecimens

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Cell Lines

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Tissue Sectioning

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Electrophoresis

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MicroRNA (miRNA) Quantification

Quantitative PCR microRNA quantitation
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Cell-Based Compound Screening

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Protein Purification

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Protein Purification and Quantification Services

Protein Purification and Quantification Services

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Bradford Assay

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RNA Extraction and Purification

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Acute Inflammation Animal Models

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Flow Cytometry Data Analysis

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Cell Line Authentication

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FACS

Fluorescence Activated Cell Sorting
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Bioanalytical Assays

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Bioanalytical Assays Services

Bioanalytical Assays Services

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Chemotaxis Assays

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Time Resolved Fluorescence

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MACS

Magnetic activated cell sorting
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Flow Cytometry Data acquisition

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Testing of Cell Lines

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Testing of Cell Lines Services

Testing of Cell Lines Services

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Endotoxin Testing

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Bicinchoninic Acid Assay

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Lead Identification and Validation

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Lead Identification and Validation Services

Lead Identification and Validation Services

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Compound Profiling

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Genotyping Services

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Genotyping Services

Genotyping Services

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DNA Services

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DNA Services

DNA Services

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Pharmacology

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Pharmacology Services

Pharmacology Services

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Microscopy

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Microscopy Services

Microscopy Services

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Optical Microscopy

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Optical Microscopy Services

Optical Microscopy Services

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Immunostaining

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Immunostaining Services

Immunostaining Services

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L. Harris

Executive Director, Discovery Biology & Translational Medicine
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Fred Hwang

Scientist, Immunology
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Agnes Lonczak

Scientist, Biology
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Weiqing Wong

Director, Proteomics
  • Positive review for Flow Cytometry:

    May 2016

    "Nanobiotec did a great job helping me plan and execute my flow cytometry experiment. Communication was great throughout the entire project and they were very helpful adjusting the data to meet my needs. The cost of my experiment was reasonable and I was completely satisfied with the quality of the data and their ability to meet my timeline for the project."

  • Positive review for Luminex Multiplex Assays:

    December 2015

    "The scientist representative of Nanobiotec was very helpful in our determination of the specific assays that we needed. Also, there was consideration of our small-lab, academic status and consequent adjustments to the costs of the study, which we very much appreciated."

  • Positive review for ADCC and CDC Assays:

    November 2015

    "Shawn is very knowledgeable, and knows what exactly he likes to do. He gives very clear instructions and advice on his project. We are very happy to have opportunities to support his drug discovery effort."

  • Positive review for Western Blot:

    March 2015

    "NANOBIOTEC was very responsive and did an excellent job on the western blots!"

NANOBIOTEC, LLC has not received any endorsements.