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NanoBiotec, LLC

43 Orders Completed
Hanover, New Jersey, US

About NanoBiotec, LLC

Founded: 2012 Type: Privately Held Size: 1-10 employees

NanoBiotec Company provides many ultrasensitive, multiplex and reliable bioassays to monitor real-time protein and gene changes in various miniscule samples from cell culture, animals and patients to accelerate discovery and development of new therapeutics.

 

Our very cost-effective services help biopharma and academic investigators to discover novel drugs and personalized medicine for cancer, inflammation, and many other unmet diseases. To meet various assay performance requirements from our clients, NanoBiotec has established a unique infrastructure with cutting-edge technologies and state-of-the-art instruments and platforms, such as

·         Flow Cytometry (CytoFlex S with 4 lasers and 13 colors)

·         Simple Western (automatic immunoblot; Capillary-based Nano-Proteomic Immunoassay);

·         Luminex-based Multiplex Immunoassay;

·         Simoa-based Ultrasensitive Immunoassay;

·         Forward and Reverse Protein Arrays (such as RPPA)

·         QuantStudio Real-Time PCR System

·         IncuCyte Zoom live cell imaging

·         LiCor Odyssey Near-Infrared Imaging System

·         Invitrogen EVOS FL Auto Cell Imaging System

·         ELISA / ELISpot/DELPHIA

·         QuantiGene/ProQuantum/ProCarta Multiplex Assay

 

Our team has unparalleled expertise in drug discovery and development, bioanalytics and proteomics. Our employees have been trained at prestigious institutes such as MIT, NIH, John Hopkins and worked over decades for multinational biopharma companies, including Novartis, BMS, Celgene, ... Our passionate scientists have extensive hands-on experiences and excellent publication records such as:

Selected Publications:

1)      Schafer, P.H., Truzzib, F. Parton, A., Wu, L., Kosek, J., Zhang, L.H., et al., "Phosphodiesterase 4 in inflammatory diseases: effects of apremilast in psoriatic blood and in dermal myofibroblasts through the PDE4/CD271 complex", Cellular Signalling. Vol. 28, pp 53-63, 2016.

2)      Yang HY, Barbi J, Wu CY, Zheng Y, Park BV, Zhang J, Bandara S, Pardoll DM, Li H, Pan F. The MicroRNA miR-17 modulates regulatory T cell activity by targeting Foxp3 Co-regulators. Immunity. Volume 45, pp83-93, 2016

3)      Henry EK, Sy CB, Inclan-Rico JM, Espinosa V, Ghanny SS, Dwyer DF, Soteropoulos P, Rivera A, Siracusa MC. Carbonic anhydrase enzymes regulate mast cell-mediated inflammation. J Exp Med. Vol.213,1663-73, 2016.

4)      Dang EV, Barbi J, Yang HY, Jinasena D, Yu H, Zheng Y, Bordman Z, Topalian SL, Semenza GL, Dang CV, Pardoll DM, Pan F. Control of Th17/Treg Balance by Hypoxia-inducible Factor 1. Cell.146, 772-84, 2011

Diversity Certificates

Small business Woman owned business

Our Services (233)


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T-Helper Cell Polarization Assays

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Natural Killer Cell Cytotoxicity Assay

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T-Cell Exhaustion Assay

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Immuno-oncology Assays

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Analysis of Tumor Infiltrating Lymphocytes (IL/TIL)

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In vitro Immune Function Testing

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Human Primary Immune Cells

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Basophil Activation Test (BAT)

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Neutrophil Oxidative Burst Assay

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T Cell Activation and Proliferation Assays

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Mixed Lymphocyte Reactions

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A common method to assess the cellular immune response is to co-culture lymphocyte populations from two individuals, (e.g. two humans or two mice of different strains) where the T cells recognize the foreign cells from the other strain, activate and proliferate. One population of cells can be restricted to being stimulator cells (e.g. a preparation of tumor cells), by pretreating them with a mitotic poison (mytomycin B), thereby turning the assay into "one-way" MLR.


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Cytokine Analysis

Price on request

The biological activity of cytokines is measured by cellular proliferation of primary cell cultures
or in vitro adapted cell lines that are dependent and/or responsive to a specific growth factor.

Other aspects of biological activity of cytokines include induction of further cytokine secretion, induction of
killing, antiviral activity, degranulation, cytotoxicity, chemotaxis, and promotion of colony formation.

In vitro assays to measure all of these activities are provided.


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Macrophage Activation Assay

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CTL Assay

Cytotoxic T Lymphocyte Assays, CTL and Patient-Matched Tumor Assays, Patient-Specific CTL Assays
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B Cell Activation Assay

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Cytotoxic T Cell Assays

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Phagocytosis Assay

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Ultra Sensitive Cytokine Detection Services

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ADCP Assay

Antibody-Dependent Cell-Mediated Phagocytosis Assay
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Antibody-Dependent Cell-Mediated Phagocytosis Assay


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Immune Checkpoint Assays

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Cytokine Storm Testing

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In vitro Immunogenicity Assays

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T-Cell Alloactivation Test

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Flow Cytometry

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• Intracellular Cytokine Secretion (ICS) – Identification and enumeration of cells that are secreting factors (cytokines)
• Cell cycle analysis – Identification of cellular division stages.
• Cell proliferation – Identification and enumeration of cellular proliferation levels.
• Cell viability and apoptosis – Identification and numeration of dead (necrotic) and dying (apoptotic) cells.
• Cell phenotyping – Identification and enumeration of different types of cells in heterogeneous mixture.
• Phosphoprotein analysis
• DNA and RNA analysis
• Translocation assays
• antibody screening
• rare event analysis, such as in dendritic cells, and stem/progenitor cells
• Cell line characterization for any markers interested
• Cell sorting
• calcium flux analysis
• disease-associated biomarker analysis and monitoring
• Multiplexed Bead-Based Immunoassays (such as cytokines, microRNAs)

Immunology

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Flow Cytometry Data Analysis

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Flow Cytometry Data Acquisition

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Phospho Flow Cytometry

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Magnetic Activated Cell Sorting (MACS)

Magnetic activated cell sorting
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Fluorescence Activated Cell Sorting (FACS)

Fluorescence Activated Cell Sorting
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Immunophenotyping

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Flow cytometry immunophenotyping is the analysis of cell surface markers using fluorochrome-conjugated monoclonal antibodies. Immunophenotyping of immune cell subsets can be used for assessing the immune status of the patient and monitor effectiveness of immunotherapies. Test results are reported as percent positive cells. Absolute cell counts are available.

Instruments: BD FACSCanto II and CytoFlex S (up to 14 colors) 


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Immunoassays

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Immunofluorescence

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Custom Antibody Arrays

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Membrane Proteome Array

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Single Cell Gel Electrophoresis

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Protein Expression Profiling

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Luminex Multiplex Assays

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We simultaneously examine multiple analytes in a single sample (as little as 10 uL per sample from human, mouse or rats), such as over 60 cytokines/chemokines, and many proteins (expression or phosphorylation) involved in signaling pathways of cancer, apoptosis, acute phase response, inflammation, immunity, diabetes, kidney toxicity, etc. We also simultaneously interrogate multiple histone H3 PTMs, transcription factors-specific DNA binding activity, and expression of genes.

Capabilities:
• Kit-based (we can buy or can be provided by the researcher), custom designed Luminex kits
• we can do extraction / homogenization/ Sonication of cell/tissue lysates, if needed
• we do optimization of protocols for commercial kits, if needed
• we analyze serum, plasma, cell lysates, cell culture supernatants
• we measure cytokines /transcription factors /protein phosphorylation/histone acetylation/ mRNA, etc
• we can measure protein and mRNA in a single sample simultaneously
• Number of samples processed in a single experiment: duplicate or triplicate, 96-well plate format

Equipment:
• Bio-Plex Pro wash stations
• BioRad Bio-Plex 200

Sample requirements:
• All samples should be Bio-Safety Level II or below.
• All samples should be submitted in ready-to-assay form.
• Any extraction or preparation procedures are the responsibility of the researchers, unless arrangements have been made in advance with us.
• Samples should be submitted in a minimum-binding 96-well plate following a standard assay design layout and accompanied by a template that outlines the sample order.
• Please provide sufficient volume for all samples to be assayed, 50-200 ul depending on duplicate or triplicate.
• Please ship samples in dry ice.

Turnaround time:
1-2 weeks or sooner, upon receiving test samples/reagent kits

Luminex®

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Gene Copy Number Analysis

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RNA-Based Immune Profiling Assay

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Ubiquitylation Analysis

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Clinical Immune Monitoring

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BrdU Assay

Bromodeoxyuridine Assay
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LDH Release Assay

Lactate Dehydrogenase Release Assay
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Comet Assay

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Custom Immunoassay Development

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qPCR

Quantitative PCR
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We offer a full range of qPCR services for mRNA and miRNA analysis.

  • Capabilities:
  • RNA isolation
  • cDNA synthesis
  • qPCR primer/ probe design
  • Reaction optimization
  • Taqman based assays
  • SYBR Green based assays
  • Multi-plex Data analysis

Resources: Ph.D. level scientists- experienced in customer support

ThermoFisher QunatStudio 6 qPCR system 


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Immuno-PCR

Price on request

Our Immuno-PCR assay combines the advantages of both ELISA and PCR, is a powerful method for detecting low quantities of protein antigens. 

Ultrasensitivity: A typical 100–10 000-fold improvement over the detection limit of the ELISA has been obtained in almost applications.

Minimal consumption of samples: 1 uL of cell culture supernatant, serum, plasma, urine, CSF, or 1 ug cell lysate is enough for the detection assay.

 


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Thermal Shift Assay (TSA)

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ADCC and CDC Assays

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Antibody-dependent cell-mediated cytotoxicity assay (ADCC)
We provide ADCC assay service to facilitate the selection of antibody drug candidates and compound candidates.

Capabilities:

• We can use PBMC or/and natural killer cells as the effector cells.
• We provide a series of target cells for testing including but not limited to

     CD20+ cells: Raji, Daudi, Ramos; 
     Her2+ cells: SK-BR-3, SK-OV-3, MCF-7; 
     EGFR+ cells: PC-3, A549, HaCaT

• Appropriate antibodies such as rituximab (anti-CD20), trastuzumab (anti-Her2) or cetuximab (anti-EGFR) are included as positive controls.
• You can also submit your target cell lines. We will culture them and test your samples for ADCC activity.
• Cell lysis is accurately quantified based on standard LDH release assay or/and calcein AM assay.
• We can optimize your antibody concentrations and effector/target ratios for testing.
• Assays are performed using a 96-well format. Each sample is tested in duplicate or triplicate.

Equipment:

• Multi-channel pipets
• Automatic pipets
• Tecan florescence and absorbance plate reader

Sample requirements:

• All samples should be Bio-Safety II or below.
• All cell lines sent to us must be shipped frozen on dry ice in cryopreservation vials or as growing cultures in flasks at ambient temperature.
• Please clearly label samples and provide sufficient volume for all samples.
• Small molecule compounds can be provided as powders or prepared as 1000x stock solutions in DMSO and shipped frozen on dry ice.
• Antibodies must be prepared in PBS at 100x – 1000x top concentration and shipped on dry ice.
• Submit a standard assay design layout and a template that outlines the sample order along with your samples.

Typical turn-around time:

• 2-3 weeks or sooner, upon receiving all materials.

CDC Assays
Target cells are suspended in media alone, media with 30-1 % serum from blood samples, or media with heat-inactivated serum. Cells are then treated with 10000-10 ng/ml test antibody at 37C for 1 h
Standard lactate dehydrogenase (LDH) release assay (CytoTox 96 Nonradioactive Cytotoxicity Assay; Promega) to measure CDC.


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Live Cell Imaging

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Compound Profiling in Cancer Cell Panels

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Simple Western™

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We offer both traditional western blot and Simple Western (capillary based nanoproteomic immunoassay –- High through-put Western Blot)

We can quantify both absolute and relative amounts of any protein (even rare or low abundance) in samples of limit amount (as low as 25 cells),

Samples can be those from cells of live animals and patients including fine needle aspirates (FNA), laser capture microdissection (LCM), FFPE samples, sorted cells, as well as serum, plasma, saliva, urine, etc.

Capabilities:
• Most sensitive immunoassay to test proteins in a minimal amount sample;
• quantify protein phosphorylation even without phospho-specific antibodies;
• We can separate proteins based on their sizes (MW) or electric charges (pI values)
• Over 100 cancer cell lines and primary cells available for testing drugs based on clients’ requirements
• we can do homogenization, and preparation of tissue lysates
• we can do protein concentration/ normalization using BCA assay
• Commonly used antibodies available
• Optimize protocols for particular antigen/target

Equipment:
• ProteinSimple Wes
• ProteinSimple Peggy
• Novex® SDS-PAGE gels
• BioRad equipment

Sample requirements:
• All samples should be Bio-Safety Level II or below.
• All samples (1 or 2 mg/ml protein) should be submitted in ready-to-assay form.
• Any extraction or preparation procedures are the responsibility of the researchers, unless arrangements have been made in advance with us.
• Samples should be submitted in a minimum-binding microfuge tubes and accompanied by a template that outlines the sample order.
• Please provide sufficient volume for all samples to be assayed
• Please ship samples in dry ice.
Turnaround time:
• 1 day -2 weeks, upon receiving test samples/ primary antibodies

Background:
Nanopro 1000 is a charge-based protein separation instrument using capillary nanoimmunoassay technology, and is used for characterizing and quantifying post-translational protein modifications in cell/ tissue/tumor lysates.

Service Details:
Many post-translational modifications result in changes in pI (isoelectric point). Nanopro 1000 can resolve a wide variety of charge variants in capillary and detect them by phospho-specific or pan-antibodies. It is therefore capable of detecting phosphorylated, acetylated, or methylated proteins. Meanwhile, this system is able to make rapid, quantitative analysis of specific proteins by using as few as 25 cells per assay.

Protein: The methods of phospho-protein detection have been validated by us, and over hundreds of other proteins can be detected upon request.
Plate formats: 96-well plate
Number of doses per compound: 9-10 dose IC50 format, or single dose
Number of replicates: Triplicates, duplicates, etc
Data analysis: % area or % phosphorylation analyzed by Compass Software

Deliverable: Gel picture and graph, analyzed data, QC results, an official final report

Sample Submission: Testing compounds can be submitted as dry powder or in DMSO stock,or cell lysate or tissue/tumor lysate in dry ice.

ProteinSimple NanoPro 1000

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Western Blot

Price on request

Simple Western - Nanoproteomic Immunoassay – Automation of Western Blot

We offer both traditional western blot and Simple Western (capillary based nanoproteomic immunoassay –- High through-put Western Blot)

We can quantify both absolute and relative amounts of any protein (even rare or low abundance) in samples of limit amount (as low as 25 cells),

Samples can be those from cells of live animals and patients including fine needle aspirates (FNA), laser capture microdissection (LCM), FFPE samples, sorted cells, as well as serum, plasma, saliva, urine, etc.

Capabilities:
• Most sensitive immunoassay to test proteins in a minimal amount sample;
• quantify protein phosphorylation even without phospho-specific antibodies;
• We can separate proteins based on their sizes (MW) or electric charges (pI values)
• Over 100 cancer cell lines and primary cells available for testing drugs based on clients’ requirements
• we can do homogenization, and preparation of tissue lysates
• we can do protein concentration/ normalization using BCA assay
• Commonly used antibodies available
• Optimize protocols for particular antigen/target

Equipment:
• ProteinSimple Wes
• ProteinSimple Peggy
• Novex® SDS-PAGE gels
• BioRad equipment

Sample requirements:
• All samples should be Bio-Safety Level II or below.
• All samples (1 or 2 mg/ml protein) should be submitted in ready-to-assay form.
• Any extraction or preparation procedures are the responsibility of the researchers, unless arrangements have been made in advance with us.
• Samples should be submitted in a minimum-binding microfuge tubes and accompanied by a template that outlines the sample order.
• Please provide sufficient volume for all samples to be assayed
• Please ship samples in dry ice.
Turnaround time:
• 1 day -2 weeks, upon receiving test samples/ primary antibodies

ProteinSimple WES

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Real-Time Cytotoxicity Assay

Price on request

We offer real-time cytotoxicity assays using our IncuCyte Live Cell Analysis System or the Invitrogen EVOS FL Auto Imaging System.

BioTek Cytation 5

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Immunocytochemistry

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3D Cell Culture Models

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Mitochondrial Stress Tests

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Clinical Biomarkers

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In-Cell Western Assay

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Our assay combines the specificity of Western blot with the reproducibility and throughput of ELISA, is a simple and cost-effective means for quantification of intracellular signaling in whole cells.

This assay involves seeding cells in 96- or 384-well microtiter plates followed by fixation/permeabilization and subsequent labeling with activation state-specific or control antibodies, infrared-conjugated secondary antibodies, and/or far-red DNA dye.

The well-level data acquired by the Odyssey® Imaging System permits accurate measurement of signal induction or inhibition, with the potential for multiple treatments, end points, and replicates on each plate.

Our assays quickly, accurately measure relative protein levels in many samples, detect proteins in situ in a relevant cellular context.

This is used for high-through put analysis of: protein phosphorylation (effects of drug compounds on signaling pathways; timing/kinetics of signal transduction), caspase-3 activation, cell surface proteins and receptor internalization, RNAi library screens, cell proliferation, etc.


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Ligand Binding Assays

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On-Cell Western Assay

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Fixed but unpermeabilized cells are stained with antibodies against extracellular protein domains, so only cell surface antigens are detected. Cell surface receptors are detected with Odyssey Infrared Imaging System.

Our assay can quantify target proteins localized to the cell surface, enabling quantitative monitoring of cell surface protein expression.

This assay can be a high throughput assay without using radioactive labeling, to quantify ligand binding to cell surface receptors, monitor receptor internalization, and evaluate of drug effects on protein trafficking, etc.


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Luminex microRNA (miRNA) Microarray

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Cell Signaling Analyses

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Cell Viability Assays

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Multiplex Gene Expression Analysis

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Multiplex nucleic acid testing allows multiplexed gene expression analysis (RNA targets) and DNA copy number variation (CNV) analysis, to measure up to 80 genes in a single sample. mRNA data is directly from cell/tissue lysates to avoid biases, sample loss, false results.


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Cell Death Assays

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ALPHAlisa

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ELISpot Assays

Enzyme-Linked ImmunoSpot Assays
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Antibody Characterization

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Reverse Phase Protein Array (RPPA)

Starting at $200.00 per sample

A high-throughput antibody-based microarray with the procedures similar to Western blots.
Proteins are extracted from tumor tissue or cells, denatured by SDS, printed on nitrocellulose- coated slides followed by antibody probe.

Capabilities:

• Currently allows for the analysis of >1000 samples using >300 different antibodies for human and mouse samples.
• Inexpensive, high-throughput method utilizing automation for increased quality and reliability
• Simple sample preparation, which is similar to Western blots
• Robust quantification due to serial dilution and replicates of samples
• characterization of cellular signaling networks under different conditions
• determination of drug selectivity and Identify therapeutic targets
• Defining regulatory mechanisms in signaling networks, including forward and feedback loops and cross talks
• Classifying patient tumors, determining prognosis and predicting responses to targeted therapies
• Understanding pharmacodynamics and biologically relevant dosing and schedule
• Determination of appropriate handling procedures for clinical samples (based on antigen stability analysis, etc.)

Equipment:

• Multi-channel pipets
• Automatic pipets
• Tecan Plate Reader
• Nano-Plotter NP2.1
• Zeptosens platform
• ZeptoREADER

Sample requirements:

• All samples should be Bio-Safety Level II or below.
• All samples (about 10-100 ul of 1 or 2 mg/ml protein) should be submitted in ready-to-assay form.
• Any extraction or preparation procedures are the responsibility of the researchers.
• Special arrangements can been made in advance with us.
• Samples should be submitted in a minimum-binding microfuge tubes and accompanied by a template that outlines the sample order.
• Please provide sufficient volume for all samples to be assayed
• Please ship samples in dry ice.

Turnaround time:

• 2 months or sooner, upon receiving test samples


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Fluorescence Polarization

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Fluorescence-Based Assays

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Cosmetic and Personal Care Product Testing

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Oxidative Stress Assays

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Human Epidermal Keratinocytes

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Human Primary Cells

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DNA Damage & Repair Assays

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Cell Cycle Analysis

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Degranulation Assay

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Mitochondrial Apoptosis Assay

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ELISA

Enzyme-linked immunosorbent assay
Price on request

Capabilities:
• Kit-based (we can buy or can be provided by the researcher), custom designed ELISA
• we can do extraction / homogenization/ Sonication of cell/tissue lysates, if needed
• we do optimization of protocols for commercial kits, if needed
• we analyze serum, plasma, cell lysates, cell culture supernatants
• we measure cytokines /transcription factors /protein phosphorylation/histone acetylation, etc
• Number of samples processed in a single experiment: duplicate or triplicate, 96-well plate format

Equipment:

• Multi-channel pipets
• Automatic pipets
• Automatic Plate washers
• Tecan Plate Reader

Sample requirements:
• All samples should be Bio-Safety Level II or below.
• All samples should be submitted in ready-to-assay form.
• Any extraction or preparation procedures are the responsibility of the researchers, unless arrangements have been made in advance with us.
• Samples should be submitted in a minimum-binding 96-well plate following a standard assay design layout and accompanied by a template that outlines the sample order.
• Please provide sufficient volume for all samples to be assayed, 50-200 ul depending on duplicate or triplicate.
• Please ship samples in dry ice.

Typical turn-around time:
• 1-2 weeks or sooner, upon receiving all materials


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Biomarker Discovery

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Autophagy Detection Assay

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Cytometric Bead Array (CBA)

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Ultra-Sensitive Simoa Assays

Price on request

Single molecule array (Simoa™) is a powerful technique that is orders of magnitude more sensitive than sandwich based immunoassay techniques. Traditional ELISA measurements are limited to pg/ml levels of detection. Our Ultrasensitive Simoa Services can achieve sensitivity as low as femtogram (fg/ml) levels, allowing the quantification of biomarkers at concentrations previously difficult or impossible to measure.

Simoa is based upon the isolation of individual immunocomplexes on paramagnetic beads using standard ELISA reagents. The main difference between Simoa and conventional immunoassays lies in the ability to trap single molecules in femtoliter-sized wells, allowing for a “digital” readout of each individual bead to determine if it is bound to the target analyte or not.

Our Simoa assay is a fully automated, ultra-sensitive, multiplexed immunoassay or Digital ELISA.  Quantification of proteins as low as femtogram (fg)/ml in the samples. The digital nature of the Simoa technology allows an average of 1000 x sensitivity increase over conventional ELISA assays with CVs < 10%.


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Transcriptomics Data Analysis

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Quantitative Western Blot Analysis

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MTT and MTS Assays

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Human Immune Cell Assays

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Autoantibody Testing

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MicroRNA (miRNA) Microarray

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ROS Assays

Reactive Oxygen Species Assays
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Lipid Peroxidation Assay

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Phosphorylation Analysis

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Compound IC50 Assays

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Caspase Assays

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Histone Deacetylase (HDAC) Assays

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Histone Acetyltransferase (HAT) Assays

Histone Acetyltransferase Assays
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Paraffin Sectioning

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Biomarker Analysis

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In vitro Wound Healing Assay

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Quantitative Proteomics

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Biochemical Compound Screening

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Anti-Microtubule Compound Screening

Microtubules

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Cancer Cell Line Panels

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Claim Substantiation

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Stem Cell Assays

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Mast Cell Degranulation Test

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3D Cell-Based Assays

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In vitro Psoriasis Models

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In vitro Immunotoxicity Testing

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In vitro Angiogenesis Assays

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Cell Migration Assays

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Cell Adhesion Assays

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Nanoproteomic Proximity Ligation Assay

Starting at $10.00 per sample per analyte

we simultaneously quantify 96 proteins in 96 samples in one experiment with our optimized primer-antibody pairs and PCR, currently focusing on cancer biomarkers.



Turnaround time: 1-2 weeks upon receiving test samples/reagent kits


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High Content Screening

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Statistical Analysis

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Phosphoproteomics

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Immunoprecipitation

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Gel Staining

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Bioanalysis

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qPCR Arrays

quantitative PCR array
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Immune Cell Assays

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Protein Quantification

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Immunohistochemistry (IHC)

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RNA Microarray

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Immunostaining

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Spectroscopy

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Cellular Health & Metabolism Assays

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Hit Identification

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Omics

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Cell Lines

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Bradford Assay

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RNA Quantification

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TaqMan Gene Expression Assays
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Arachidonic acid-induced ear edema in ICR mice
Croton oil-induced ear edema in ICR mice
Phorbol ester-induced ear edema in ICR mice
Carrageen-induced paw edema in mice
Testosterone induced benign prostatic hyperplasia
Acetic acid induced pain in mice
Cecal ligation and puncture induced sepsis in mice


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Dermatology Animal Models

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Animal Models of Disease

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Osteoarthritis Animal Models

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Skin Fibrosis Animal Models

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LH

L. Harris

Ph.D. Executive Director, Discovery Biology & Translational Development
EH

Everett Henry

Ph.D. Scientist, Immunology & Cytometry
AL

Agnes Lonczak

BSc, M. Sc., Scientist, Cell Molecular Biology
FP

Frank Pan

Ph.D., M.D. Executive Director, Cellular & Molecular Immuno-Oncology
WW

Weiqing Wong

Ph.D. Executive Director, Proteomics and Immuoassays
WZ

Wanying Zhang

B.Sc. M.Sc, Scientist, Pharmacology
  • Positive review for Fluorescence Activated Cell Sorting (FACS):

    May 2019

    "I was very happy with Nanobiotec for my FACS project. They were very responsive and adaptable to what turned out to be a more challenging project than I had originally anticipated, with quick response times to emails and clearly written reports on ongoing progress."

  • Positive review for Flow Cytometry:

    May 2016

    "Nanobiotec did a great job helping me plan and execute my flow cytometry experiment. Communication was great throughout the entire project and they were very helpful adjusting the data to meet my needs. The cost of my experiment was reasonable and I was completely satisfied with the quality of the data and their ability to meet my timeline for the project."

  • Positive review for Luminex Multiplex Assays:

    December 2015

    "The scientist representative of Nanobiotec was very helpful in our determination of the specific assays that we needed. Also, there was consideration of our small-lab, academic status and consequent adjustments to the costs of the study, which we very much appreciated."

  • Positive review for ADCC and CDC Assays:

    November 2015

    "Shawn is very knowledgeable, and knows what exactly he likes to do. He gives very clear instructions and advice on his project. We are very happy to have opportunities to support his drug discovery effort."

  • Positive review for Western Blot:

    March 2015

    "NANOBIOTEC was very responsive and did an excellent job on the western blots!"

  • ZW

    Zhiyong WangFebruary 2019

    NanoBiotec has supported us for several projects, using their automatic Western blot analyses (simple Western), Luminex-based assay and flow cytometric assay platforms. All projects were successfully completed in an efficient and timely manner. The data summary/report were very well and accurately written. Their pricing were also very reasonable. We are quite happy with their quality of work and would definitely recommend this company to all investigators as a valuable partner.

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