The MUSC Proteomics Center is focused on clinical and translational proteomics and development of centralized diagnostics. It is comprised of independent investigators, two core laboratories (Mass Spectrometry Facility and MS Imaging Research Center), and supported by external funding, as well as the MUSC Provost Office, College of Medicine, Hollings Cancer Center, NIH-COBRE and SmartState funding. Mass spectrometry is the key analytical method for proteomics research, and the Center has recently obtained significant upgrades to an extensive mass spectrometry facility. Current instrumentation includes three ion trap instruments (one Thermo Orbitrap Elite with ETD, two Thermo LTQ's) for analysis of post-translational modifications like phosphorylation and glycosylation. Quantitative proteomics of cultured cells using SILAC and iTRAQ/TMT labeling, as well as characterization of multiple types of post-translational modifications, is the primary emphasis for use of the Orbitrap. Also in the facility are a dual-source Bruker Solarix 7T Fourier Transform-ICR mass spectrometer and two Autoflex MALDI-TOF instruments used primarily for MALDI tissue imaging. The MALDI tissue imaging approaches are histopathology directed, and used for two-dimensional spatial profiling of proteins, lipids, glycans, metabolites and small molecule drugs. The Center leverages high-performance computing resources with modern protein search algorithms, mass spectrometry based quantification analysis, statistical analysis (frequentist or Bayesian techniques), data-driven systems biology, -omic integration techniques and classifier development (machine learning based) to generate actionable and hypothesis driving results.
MALDI FT-ICR Tissue Imaging
o MALDI Fourier Transform Ion Cyclotron Resonance mass spectrometer (7.4 T Solarix, Bruker Daltonics)
o TM-Sprayer (HTX Imaging) – for fine homogenous enzymatic and MALDI matrix application
o The instrument is built with a cooling source to stabilize sialic acids following ionization
o Laser spot size of 25 µm.
• Standard Sample Requirements:
o FFPE tissue sections, 5-6 µm thickness on positively charged microscope slides
o Microscope slides must be (75x25x1mm) to fit into the sample carriers
o A sample is defined as the tissue(s) mounted on one positively charged microscope slide.
o Standard imaging runs use 100-150 µm spatial resolution, but depend on tissue features. Spatial resolution should be discussed at time of service initiation.
• Turnaround times (3-4 weeks for <10 samples, depending on current queue)
• Pricing: Standard N-glycan Imaging
o $1500 per tissue(s) on a single positively charge microscope slide, allowing up to 8 hours instrument run time per sample
o $150/hr for analysis runs over 8 hours
o TMAs $3000 per 150 cores
o 10-20 samples, add $500 startup fee
o >30 samples, please inquire
o All sample preparation including antigen retrieval, dewaxing, scanning, PNGase F treatment, MALDI matrix application and a maximum of 8 hours of instrument run time per sample
o Panel of images corresponding by accurate mass to N-Glycan Database
o H&E stained slide after imaging
o FTP data transfer
o Data storage 1 year
• Pricing Option 1: Statistical Analysis (<10 samples)
o Statistical analysis between target regions or samples $2000
o Turnaround time: 3 weeks
o Deliverables: Principal component analysis, image segmentation, expression ratios, receiver operating curve results
• Pricing Option 2: Structure Information
o MS/MS structure information: $500 per each N-glycan
MUSC Proteomics Center has not received any reviews.
MUSC Proteomics Center has not received any endorsements.